CN1714147B - 无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系 - Google Patents
无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系 Download PDFInfo
- Publication number
- CN1714147B CN1714147B CN2003801036918A CN200380103691A CN1714147B CN 1714147 B CN1714147 B CN 1714147B CN 2003801036918 A CN2003801036918 A CN 2003801036918A CN 200380103691 A CN200380103691 A CN 200380103691A CN 1714147 B CN1714147 B CN 1714147B
- Authority
- CN
- China
- Prior art keywords
- protein
- cell
- concentration
- protein concentration
- critical
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 238000000034 method Methods 0.000 title claims abstract description 39
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 99
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 96
- 210000002966 serum Anatomy 0.000 claims abstract description 17
- 230000006978 adaptation Effects 0.000 claims abstract description 14
- 239000012634 fragment Substances 0.000 claims abstract description 3
- 210000004027 cell Anatomy 0.000 claims description 75
- 239000002609 medium Substances 0.000 claims description 40
- 239000006228 supernatant Substances 0.000 claims description 17
- 238000011081 inoculation Methods 0.000 claims description 9
- 230000003833 cell viability Effects 0.000 claims description 8
- 206010035226 Plasma cell myeloma Diseases 0.000 claims description 7
- 238000004113 cell culture Methods 0.000 claims description 7
- 244000309466 calf Species 0.000 claims description 6
- 210000004962 mammalian cell Anatomy 0.000 claims description 6
- 108010013381 Porins Proteins 0.000 claims description 4
- 102000007056 Recombinant Fusion Proteins Human genes 0.000 claims description 4
- 108010008281 Recombinant Fusion Proteins Proteins 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 201000000050 myeloid neoplasm Diseases 0.000 claims description 4
- 102000007739 porin activity proteins Human genes 0.000 claims description 4
- 108090000765 processed proteins & peptides Proteins 0.000 claims description 4
- 239000006143 cell culture medium Substances 0.000 claims description 3
- 238000012258 culturing Methods 0.000 claims description 3
- 229920001184 polypeptide Polymers 0.000 claims description 3
- 102000004196 processed proteins & peptides Human genes 0.000 claims description 3
- 241000124008 Mammalia Species 0.000 abstract description 5
- 235000018102 proteins Nutrition 0.000 description 64
- 230000012010 growth Effects 0.000 description 8
- 230000003044 adaptive effect Effects 0.000 description 6
- 239000012980 RPMI-1640 medium Substances 0.000 description 4
- 238000003745 diagnosis Methods 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 238000013207 serial dilution Methods 0.000 description 4
- 208000034578 Multiple myelomas Diseases 0.000 description 3
- 238000004364 calculation method Methods 0.000 description 3
- 238000010276 construction Methods 0.000 description 3
- 101100230376 Acetivibrio thermocellus (strain ATCC 27405 / DSM 1237 / JCM 9322 / NBRC 103400 / NCIMB 10682 / NRRL B-4536 / VPI 7372) celI gene Proteins 0.000 description 2
- 241000699802 Cricetulus griseus Species 0.000 description 2
- 206010028980 Neoplasm Diseases 0.000 description 2
- 108010009736 Protein Hydrolysates Proteins 0.000 description 2
- 210000001672 ovary Anatomy 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000003531 protein hydrolysate Substances 0.000 description 2
- 238000001890 transfection Methods 0.000 description 2
- 208000023275 Autoimmune disease Diseases 0.000 description 1
- 101100264195 Caenorhabditis elegans app-1 gene Proteins 0.000 description 1
- 102100022641 Coagulation factor IX Human genes 0.000 description 1
- 229920000858 Cyclodextrin Polymers 0.000 description 1
- 238000002965 ELISA Methods 0.000 description 1
- 102000003951 Erythropoietin Human genes 0.000 description 1
- 108090000394 Erythropoietin Proteins 0.000 description 1
- 108010076282 Factor IX Proteins 0.000 description 1
- CEAZRRDELHUEMR-URQXQFDESA-N Gentamicin Chemical compound O1[C@H](C(C)NC)CC[C@@H](N)[C@H]1O[C@H]1[C@H](O)[C@@H](O[C@@H]2[C@@H]([C@@H](NC)[C@@](C)(O)CO2)O)[C@H](N)C[C@@H]1N CEAZRRDELHUEMR-URQXQFDESA-N 0.000 description 1
- 229930182566 Gentamicin Natural products 0.000 description 1
- 102000002265 Human Growth Hormone Human genes 0.000 description 1
- 108010000521 Human Growth Hormone Proteins 0.000 description 1
- 239000000854 Human Growth Hormone Substances 0.000 description 1
- 102000013266 Human Regular Insulin Human genes 0.000 description 1
- 108010090613 Human Regular Insulin Proteins 0.000 description 1
- 102000004877 Insulin Human genes 0.000 description 1
- 108090001061 Insulin Proteins 0.000 description 1
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 description 1
- 108090000190 Thrombin Proteins 0.000 description 1
- 102000003978 Tissue Plasminogen Activator Human genes 0.000 description 1
- 108090000373 Tissue Plasminogen Activator Proteins 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 239000003708 ampul Substances 0.000 description 1
- 229960000182 blood factors Drugs 0.000 description 1
- 150000004648 butanoic acid derivatives Chemical class 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 230000004663 cell proliferation Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 229940105423 erythropoietin Drugs 0.000 description 1
- DNJIEGIFACGWOD-UHFFFAOYSA-N ethyl mercaptane Natural products CCS DNJIEGIFACGWOD-UHFFFAOYSA-N 0.000 description 1
- 229960004222 factor ix Drugs 0.000 description 1
- 239000006052 feed supplement Substances 0.000 description 1
- 238000000855 fermentation Methods 0.000 description 1
- 230000004151 fermentation Effects 0.000 description 1
- 229960002518 gentamicin Drugs 0.000 description 1
- ZDXPYRJPNDTMRX-UHFFFAOYSA-N glutamine Natural products OC(=O)C(N)CCC(N)=O ZDXPYRJPNDTMRX-UHFFFAOYSA-N 0.000 description 1
- 229940038563 human regular insulin Drugs 0.000 description 1
- 210000004408 hybridoma Anatomy 0.000 description 1
- 239000000413 hydrolysate Substances 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- 229910052744 lithium Inorganic materials 0.000 description 1
- -1 lymphokine Proteins 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 230000010412 perfusion Effects 0.000 description 1
- OXCMYAYHXIHQOA-UHFFFAOYSA-N potassium;[2-butyl-5-chloro-3-[[4-[2-(1,2,4-triaza-3-azanidacyclopenta-1,4-dien-5-yl)phenyl]phenyl]methyl]imidazol-4-yl]methanol Chemical compound [K+].CCCCC1=NC(Cl)=C(CO)N1CC1=CC=C(C=2C(=CC=CC=2)C2=N[N-]N=N2)C=C1 OXCMYAYHXIHQOA-UHFFFAOYSA-N 0.000 description 1
- 230000001185 psoriatic effect Effects 0.000 description 1
- HFHDHCJBZVLPGP-UHFFFAOYSA-N schardinger α-dextrin Chemical compound O1C(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC(C(O)C2O)C(CO)OC2OC(C(C2O)O)C(CO)OC2OC2C(O)C(O)C1OC2CO HFHDHCJBZVLPGP-UHFFFAOYSA-N 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 229960004072 thrombin Drugs 0.000 description 1
- 229960000187 tissue plasminogen activator Drugs 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Abstract
Description
Claims (8)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU20020239A CU23097A1 (es) | 2002-10-23 | 2002-10-23 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
CU239/2002 | 2002-10-23 | ||
PCT/CU2003/000012 WO2004038010A1 (es) | 2002-10-23 | 2003-10-22 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
Publications (2)
Publication Number | Publication Date |
---|---|
CN1714147A CN1714147A (zh) | 2005-12-28 |
CN1714147B true CN1714147B (zh) | 2011-01-12 |
Family
ID=40134843
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN2003801036918A Expired - Lifetime CN1714147B (zh) | 2002-10-23 | 2003-10-22 | 无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系 |
Country Status (27)
Country | Link |
---|---|
US (2) | US20060036082A1 (zh) |
EP (2) | EP2363418B1 (zh) |
JP (1) | JP4674087B2 (zh) |
KR (1) | KR101186048B1 (zh) |
CN (1) | CN1714147B (zh) |
AR (1) | AR041645A1 (zh) |
AT (1) | ATE502103T1 (zh) |
AU (1) | AU2003273727B2 (zh) |
CA (1) | CA2503315C (zh) |
CU (1) | CU23097A1 (zh) |
DE (1) | DE60336415D1 (zh) |
DK (1) | DK1564285T3 (zh) |
EA (1) | EA007465B1 (zh) |
EC (1) | ECSP055740A (zh) |
ES (2) | ES2624279T3 (zh) |
GE (1) | GEP20084444B (zh) |
HK (1) | HK1084414A1 (zh) |
HR (1) | HRP20050760A2 (zh) |
LT (1) | LT5354B (zh) |
LV (1) | LV13361B (zh) |
MY (1) | MY144088A (zh) |
NZ (1) | NZ540171A (zh) |
PE (1) | PE20040552A1 (zh) |
PT (1) | PT1564285E (zh) |
TN (1) | TNSN05116A1 (zh) |
UY (1) | UY28037A1 (zh) |
WO (1) | WO2004038010A1 (zh) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104673754B (zh) * | 2015-02-14 | 2018-03-02 | 百泰生物药业有限公司 | 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子 |
CN104651314B (zh) * | 2015-02-14 | 2018-06-19 | 百泰生物药业有限公司 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1130193A (zh) * | 1994-11-18 | 1996-09-04 | 分子免疫中心 | 识别表皮生长因子受体(egf-r)的人源化和嵌合体的单克隆抗体及其诊断和治疗用途 |
Family Cites Families (18)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3694263A (en) * | 1970-10-23 | 1972-09-26 | Joseph J Korn Sr | Swimming pool cleaning methods and apparatus therefor |
US3886616A (en) * | 1972-12-06 | 1975-06-03 | Fay A Hayes | Hand propelled swimming pool cleaner |
US4275474A (en) * | 1979-04-30 | 1981-06-30 | Woodard Randle C | Vacuum head for swimming pool cleaning system |
US4240174A (en) * | 1979-07-30 | 1980-12-23 | Scott Jeffrey L | Self-contained mobile pool cleaning apparatus |
US4430214A (en) * | 1982-09-15 | 1984-02-07 | Baker Marvin E | Strainer mill for swimming pool pump intake |
AU2986484A (en) * | 1983-06-27 | 1985-01-03 | Monash University | Leptospira strains grown in protein free medium |
US4558479A (en) * | 1984-01-26 | 1985-12-17 | Alopex Industries, Inc. | Pool cleaner |
US4683599A (en) * | 1984-03-30 | 1987-08-04 | Fahet Nv | Automatic pool cleaner fitting |
US4581075A (en) * | 1985-03-15 | 1986-04-08 | Maxi-Sweep, Inc. | Self-propelled water borne pool cleaner |
AU6291090A (en) | 1989-09-29 | 1991-04-28 | Atomic Energy Of Canada Limited | Infrared-based gas detector |
JP2696001B2 (ja) | 1991-04-15 | 1998-01-14 | 財団法人化学及血清療法研究所 | 組換え蛋白質産生用培地 |
EP0531562A1 (de) | 1991-09-11 | 1993-03-17 | Doerr, Hans-Wilhelm, Prof. Dr. med. | Kultivierung von Säugetierzellen |
PT784684E (pt) * | 1994-09-16 | 2007-11-23 | Cancer Res Fund Of Contra Cost | ''péptidos recontinantes derivados do anticorpo mc3 anti-ba46, métodos para a sua utilização e métodos de hmnanização de péptidos do anticorpo'' |
JP3158157B2 (ja) | 1994-11-10 | 2001-04-23 | バクスター・アクチエンゲゼルシャフト | 無蛋白培養における生物製剤の製造法 |
AU703484B2 (en) | 1995-02-23 | 1999-03-25 | Quest International Services B.V. | Peptides for tissue and cell culture media |
AUPN442295A0 (en) | 1995-07-26 | 1995-08-17 | Commonwealth Scientific And Industrial Research Organisation | Regulated autocrine growth of mammalian cells |
CU22584A1 (es) | 1995-11-17 | 1999-11-03 | Centro Inmunologia Molecular | Composiciones farmacéuticas que contienen un anticuerpo monoclonal que reconoce el antígeno de diferenciación leucocitario humano cd6 y sus usos para el diagnóstico y tratamiento de la psoriasis |
AT407255B (de) * | 1997-06-20 | 2001-02-26 | Immuno Ag | Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons |
-
2002
- 2002-10-23 CU CU20020239A patent/CU23097A1/es unknown
-
2003
- 2003-10-10 PE PE2003001035A patent/PE20040552A1/es not_active Application Discontinuation
- 2003-10-16 AR ARP030103776A patent/AR041645A1/es active IP Right Grant
- 2003-10-17 MY MYPI20033959A patent/MY144088A/en unknown
- 2003-10-22 CA CA2503315A patent/CA2503315C/en not_active Expired - Lifetime
- 2003-10-22 DE DE60336415T patent/DE60336415D1/de not_active Expired - Lifetime
- 2003-10-22 EP EP11155076.0A patent/EP2363418B1/en not_active Expired - Lifetime
- 2003-10-22 GE GEAP20038815A patent/GEP20084444B/en unknown
- 2003-10-22 AT AT03757654T patent/ATE502103T1/de active
- 2003-10-22 ES ES11155076.0T patent/ES2624279T3/es not_active Expired - Lifetime
- 2003-10-22 NZ NZ540171A patent/NZ540171A/en not_active IP Right Cessation
- 2003-10-22 EP EP03757654A patent/EP1564285B1/en not_active Expired - Lifetime
- 2003-10-22 PT PT03757654T patent/PT1564285E/pt unknown
- 2003-10-22 ES ES03757654T patent/ES2360049T3/es not_active Expired - Lifetime
- 2003-10-22 WO PCT/CU2003/000012 patent/WO2004038010A1/es active IP Right Grant
- 2003-10-22 CN CN2003801036918A patent/CN1714147B/zh not_active Expired - Lifetime
- 2003-10-22 DK DK03757654.3T patent/DK1564285T3/da active
- 2003-10-22 AU AU2003273727A patent/AU2003273727B2/en not_active Expired
- 2003-10-22 KR KR1020057006995A patent/KR101186048B1/ko active IP Right Grant
- 2003-10-22 US US10/532,269 patent/US20060036082A1/en not_active Abandoned
- 2003-10-22 JP JP2004545689A patent/JP4674087B2/ja not_active Expired - Lifetime
- 2003-10-22 EA EA200500695A patent/EA007465B1/ru not_active IP Right Cessation
- 2003-10-23 UY UY28037A patent/UY28037A1/es not_active Application Discontinuation
-
2005
- 2005-04-22 EC EC2005005740A patent/ECSP055740A/es unknown
- 2005-04-22 TN TNP2005000116A patent/TNSN05116A1/en unknown
- 2005-05-19 LT LT2005053A patent/LT5354B/lt not_active IP Right Cessation
- 2005-05-23 LV LVP-05-61A patent/LV13361B/en unknown
- 2005-09-02 HR HR20050760A patent/HRP20050760A2/xx not_active Application Discontinuation
-
2006
- 2006-04-13 HK HK06104515.2A patent/HK1084414A1/xx not_active IP Right Cessation
-
2010
- 2010-04-13 US US12/759,208 patent/US20100197011A1/en not_active Abandoned
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1130193A (zh) * | 1994-11-18 | 1996-09-04 | 分子免疫中心 | 识别表皮生长因子受体(egf-r)的人源化和嵌合体的单克隆抗体及其诊断和治疗用途 |
Non-Patent Citations (2)
Title |
---|
Serum-Free and Protein-Free Media for Hybridoma Culture.GIBCO invitrogen 公司在线数据.2002,http://ivitrogen.com/contents/sfs/manuals/3913.pdf. * |
Sinacore 等人.Adaption of Mammlian cells to serum-free Media.Mol.Biotechology15.2000,15253-254. * |
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
BAZIN | Production of rat monoclonal antibodies with the LOU rat non secreting IR983F myeloma cell line | |
Brown et al. | Process development for the production of recombinant antibodies using the glutamine synthetase (GS) system | |
Kim et al. | Characterization of chimeric antibody producing CHO cells in the course of dihydrofolate reductase‐mediated gene amplification and their stability in the absence of selective pressure | |
CN105378086B (zh) | 用于控制重组蛋白质中c端赖氨酸、半乳糖和唾液酸含量的制造方法 | |
CN104024423B (zh) | 用于降低抗体异质性的方法和产生其抗体的方法 | |
EP0043718A2 (en) | Improvements in or relating to cell lines | |
JP2023126248A (ja) | 細胞培養培地 | |
TW200831669A (en) | Cell culture improvements | |
EP0014519A2 (en) | Cell lines, process for preparing them and process for producing antibodies | |
BR112012002151A2 (pt) | Métodos para expressar uma desintegrina e metaloproteinase com proteína de motivo trombospondina, para produzir uma composição de desintegrina e metaloproteinase com proteína de motivo trombospondina, para produzir uma composição de desintegrina e metaloproteinase com proteína de motivo trombospondina,e, composição de proteína de desintegrina e metaloproteinase com proteína de motivo trombospondina. | |
JP2011518790A5 (zh) | ||
CN110418846A (zh) | 细胞培养物中产生的抗体的总去岩藻糖基化糖型的控制 | |
CN109153716A (zh) | 调节单克隆抗体组合物中的非岩藻糖基化物类 | |
Siraganian et al. | [2] Methods of enhancing the frequency of antigen-specific hybridomas | |
JP2017516484A (ja) | タンパク質生産のための細胞培養工程 | |
CN1714147B (zh) | 无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系 | |
US20200323958A1 (en) | Methods for producing protein products | |
CN87102823A (zh) | 生产肽的方法,用于生产肽的重组质粒和用该重组质粒转化的动物细胞 | |
CN111373028B (zh) | 蛋白质的生产方法 | |
CN1328157A (zh) | 新的基因工程抗体真核高效表达***、其制备方法及其应用 | |
CA2039168A1 (en) | Neutralizing monoclonal antibody to infectious pancreatic necrosis virus | |
BRPI0315565B1 (pt) | Método para se obter uma linhagem celular de mieloma nso que contém uma sequência que codifica um polipeptídeo recombinante ou uma proteína recombinante adaptada para crescer em meio livre de soro e proteína, e, uso do método | |
TH69081A (th) | วิธีการสำหรับทำให้ได้สายพันธุ์เซลในอาหารเลี้ยงที่ปราศจากโปรตีนและสายพันธุ์เซลที่ได้จากวิธีการนั้น |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: DE Ref document number: 1084414 Country of ref document: HK |
|
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
REG | Reference to a national code |
Ref country code: HK Ref legal event code: GR Ref document number: 1084414 Country of ref document: HK |
|
TR01 | Transfer of patent right |
Effective date of registration: 20180726 Address after: Cuba Havana Co-patentee after: BIOTECH PHARMACEUTICAL Co.,Ltd. Patentee after: CENTRO DE INMUNOLOGIA MOLECULAR Address before: Cuba Havana Patentee before: Centro De Inmunologia Molecular |
|
TR01 | Transfer of patent right | ||
CX01 | Expiry of patent term |
Granted publication date: 20110112 |
|
CX01 | Expiry of patent term |