UY28037A1 - Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo - Google Patents

Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo

Info

Publication number
UY28037A1
UY28037A1 UY28037A UY28037A UY28037A1 UY 28037 A1 UY28037 A1 UY 28037A1 UY 28037 A UY28037 A UY 28037A UY 28037 A UY28037 A UY 28037A UY 28037 A1 UY28037 A1 UY 28037A1
Authority
UY
Uruguay
Prior art keywords
cellular lines
grow
free
free media
obtaining
Prior art date
Application number
UY28037A
Other languages
English (en)
Inventor
Rolando Perez Rodriguez
Adolfo Castillo Vitlloch
Svieta Vicores Sarazola
Tammy Boggiano Ayo
Luis Rojas Del Calvo
Original Assignee
Centro Inmunologia Molecular
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centro Inmunologia Molecular filed Critical Centro Inmunologia Molecular
Publication of UY28037A1 publication Critical patent/UY28037A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
    • C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
    • C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0693—Tumour cells; Cancer cells
    • C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95—Protein-free medium and culture conditions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • Oncology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Cell Biology (AREA)
  • Hematology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)

Abstract

Método de obtención de células de mamífero adaptadas a crecer en medio libre de suero y proteínas, que incluye dos etapas: una, revela el intervalo crítico de concentración de proteína en el cual las células deban crecer para ganar la capacidad de sobrevivir en medio libre de proteínas, y otra las disminuciones subsiguientes de la concentración para que no afecten la viabilidad ni el tiempo de doblaje celular. Se revelan las líneas celulares que crecen establemente por al menos 40 generaciones. Los clones expresan los anticuerpos anti receptor de EGF hR3, el anti CD6 T1hT humanizados y el anticuerpo quimérico anti CD3 T3Q, además de los fragmentos de dichos anticuerpos.
UY28037A 2002-10-23 2003-10-23 Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo UY28037A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CU20020239A CU23097A1 (es) 2002-10-23 2002-10-23 Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método

Publications (1)

Publication Number Publication Date
UY28037A1 true UY28037A1 (es) 2004-04-30

Family

ID=40134843

Family Applications (1)

Application Number Title Priority Date Filing Date
UY28037A UY28037A1 (es) 2002-10-23 2003-10-23 Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo

Country Status (27)

Country Link
US (2) US20060036082A1 (es)
EP (2) EP2363418B1 (es)
JP (1) JP4674087B2 (es)
KR (1) KR101186048B1 (es)
CN (1) CN1714147B (es)
AR (1) AR041645A1 (es)
AT (1) ATE502103T1 (es)
AU (1) AU2003273727B2 (es)
CA (1) CA2503315C (es)
CU (1) CU23097A1 (es)
DE (1) DE60336415D1 (es)
DK (1) DK1564285T3 (es)
EA (1) EA007465B1 (es)
EC (1) ECSP055740A (es)
ES (2) ES2624279T3 (es)
GE (1) GEP20084444B (es)
HK (1) HK1084414A1 (es)
HR (1) HRP20050760A2 (es)
LT (1) LT5354B (es)
LV (1) LV13361B (es)
MY (1) MY144088A (es)
NZ (1) NZ540171A (es)
PE (1) PE20040552A1 (es)
PT (1) PT1564285E (es)
TN (1) TNSN05116A1 (es)
UY (1) UY28037A1 (es)
WO (1) WO2004038010A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104673754B (zh) * 2015-02-14 2018-03-02 百泰生物药业有限公司 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子
CN104651314B (zh) * 2015-02-14 2018-06-19 百泰生物药业有限公司 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子

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US3694263A (en) * 1970-10-23 1972-09-26 Joseph J Korn Sr Swimming pool cleaning methods and apparatus therefor
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US3886616A (en) * 1972-12-06 1975-06-03 Fay A Hayes Hand propelled swimming pool cleaner
US4275474A (en) * 1979-04-30 1981-06-30 Woodard Randle C Vacuum head for swimming pool cleaning system
US4240174A (en) * 1979-07-30 1980-12-23 Scott Jeffrey L Self-contained mobile pool cleaning apparatus
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WO1991005240A1 (en) 1989-09-29 1991-04-18 Atomic Energy Of Canada Limited Infrared-based gas detector
JP2696001B2 (ja) 1991-04-15 1998-01-14 財団法人化学及血清療法研究所 組換え蛋白質産生用培地
EP0531562A1 (de) 1991-09-11 1993-03-17 Doerr, Hans-Wilhelm, Prof. Dr. med. Kultivierung von Säugetierzellen
ATE370235T1 (de) * 1994-09-16 2007-09-15 Cancer Res Fund Of Contra Cost Rekombinantpeptide hergeleitet vom mc3 antikörper gegen ba46, verfahren zu deren verwendung und verfahren zur humanisierung von antikörperpeptiden
ES2378409T3 (es) 1994-11-10 2012-04-12 Baxter Healthcare S.A. Método para producir sustancias biológicas en cultivo sin proteínas
DE69608668T2 (de) 1995-02-23 2001-02-01 Quest Int Peptide für gewebe- und zellkulturmedien
AUPN442295A0 (en) 1995-07-26 1995-08-17 Commonwealth Scientific And Industrial Research Organisation Regulated autocrine growth of mammalian cells
CU22584A1 (es) 1995-11-17 1999-11-03 Centro Inmunologia Molecular Composiciones farmacéuticas que contienen un anticuerpo monoclonal que reconoce el antígeno de diferenciación leucocitario humano cd6 y sus usos para el diagnóstico y tratamiento de la psoriasis
AT407255B (de) * 1997-06-20 2001-02-26 Immuno Ag Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons

Also Published As

Publication number Publication date
EP2363418A2 (en) 2011-09-07
LV13361B (en) 2006-02-20
AU2003273727A1 (en) 2004-05-13
LT5354B (lt) 2006-07-25
ATE502103T1 (de) 2011-04-15
EP2363418B1 (en) 2017-02-08
AU2003273727B2 (en) 2008-04-03
ES2624279T3 (es) 2017-07-13
PT1564285E (pt) 2011-04-20
NZ540171A (en) 2008-04-30
BR0315565B1 (pt) 2022-01-25
WO2004038010A1 (es) 2004-05-06
MY144088A (en) 2011-08-15
HRP20050760A2 (en) 2006-08-31
CA2503315A1 (en) 2004-05-06
EA200500695A1 (ru) 2005-10-27
JP4674087B2 (ja) 2011-04-20
PE20040552A1 (es) 2004-10-26
DK1564285T3 (da) 2011-05-16
EA007465B1 (ru) 2006-10-27
KR101186048B1 (ko) 2012-09-25
BR0315565A (pt) 2005-08-23
ECSP055740A (es) 2005-09-20
US20060036082A1 (en) 2006-02-16
GEP20084444B (en) 2008-08-10
DE60336415D1 (de) 2011-04-28
ES2360049T3 (es) 2011-05-31
HK1084414A1 (en) 2006-07-28
EP1564285A1 (en) 2005-08-17
CU23097A1 (es) 2005-11-18
KR20050088403A (ko) 2005-09-06
CN1714147A (zh) 2005-12-28
US20100197011A1 (en) 2010-08-05
JP2006503879A (ja) 2006-02-02
CA2503315C (en) 2014-02-18
CN1714147B (zh) 2011-01-12
LT2005053A (en) 2006-04-25
EP2363418A3 (en) 2011-11-16
EP1564285B1 (en) 2011-03-16
TNSN05116A1 (en) 2007-05-14
AR041645A1 (es) 2005-05-26

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Effective date: 20160531