CU23097A1 - Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método - Google Patents
Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este métodoInfo
- Publication number
- CU23097A1 CU23097A1 CU20020239A CU20020239A CU23097A1 CU 23097 A1 CU23097 A1 CU 23097A1 CU 20020239 A CU20020239 A CU 20020239A CU 20020239 A CU20020239 A CU 20020239A CU 23097 A1 CU23097 A1 CU 23097A1
- Authority
- CU
- Cuba
- Prior art keywords
- present
- grow
- protein
- free
- cells
- Prior art date
Links
- 230000001413 cellular effect Effects 0.000 title 1
- 239000004017 serum-free culture medium Substances 0.000 title 1
- 210000004027 cell Anatomy 0.000 abstract 4
- 102000004169 proteins and genes Human genes 0.000 abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 3
- 210000004962 mammalian cell Anatomy 0.000 abstract 2
- 230000006978 adaptation Effects 0.000 abstract 1
- 230000007423 decrease Effects 0.000 abstract 1
- 239000012634 fragment Substances 0.000 abstract 1
- 239000012679 serum free medium Substances 0.000 abstract 1
- 230000035899 viability Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Abstract
La actual invención se relaciona con un método de obtención de células de mamífero adaptadas a crecer en medio libre de suero y proteínas, el peocedimiento incluye un proceso en dos etapas de adaptación para crecer en esa condición. La actual invención revela la existencia de un intervalo crítico de concentración de proteína en el cual las células deban crecer para ganar la capacidad de sobrevivir en medio libre de proteínas, una vez que las células hayan crecido en las concentraciones críticas del intervalo, las disminuciones subsiguientes de la concentración no afecten la viabilidad ni el tiempo de doblaje celular. El intervalo crítico de la concentración de la proteína es específico para cada línea celular. La presente invención también revela líneas celulares de mamíferos que crecen establemente en condiciones de medio libre de suero y proteínas por al menos 40 generaciones. Adicionalmente, los clones revelados en la presente invenci´´on expresan un producto recombinante. Los clones de la presente invención expresan los anticuerpos anti receptor de EGF hR3, el anti CD6 T1hT humanizados y el anticuerpo quimérico anti CD3 T3Q, además de los fragmentos de dichos anticuerpos.
Priority Applications (31)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU20020239A CU23097A1 (es) | 2002-10-23 | 2002-10-23 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
PE2003001035A PE20040552A1 (es) | 2002-10-23 | 2003-10-10 | Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo |
ARP030103776A AR041645A1 (es) | 2002-10-23 | 2003-10-16 | Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo |
MYPI20033959A MY144088A (en) | 2002-10-23 | 2003-10-17 | Procedure for obtaining cell lines in protein-free media and cell line obtained by the method |
BRPI0315565-0A BRPI0315565B1 (pt) | 2002-10-23 | 2003-10-22 | Método para se obter uma linhagem celular de mieloma nso que contém uma sequência que codifica um polipeptídeo recombinante ou uma proteína recombinante adaptada para crescer em meio livre de soro e proteína, e, uso do método |
PT03757654T PT1564285E (pt) | 2002-10-23 | 2003-10-22 | Processo para a obtenção de linhas de células de mieoloma de mamífero recombinantes adaptadas para crescer em meio isento de soro e de proteínas |
ES11155076.0T ES2624279T3 (es) | 2002-10-23 | 2003-10-22 | Procedimiento para obtener líneas celulares de mieloma de mamífero recombinantes adaptadas al crecimiento en medios sin suero ni proteínas |
JP2004545689A JP4674087B2 (ja) | 2002-10-23 | 2003-10-22 | 蛋白質非含有培地中での細胞株の取得方法およびこうして取得された細胞株 |
EP11155076.0A EP2363418B1 (en) | 2002-10-23 | 2003-10-22 | Method of obtaining recombinant mammalian myeloma cell lines adapted to growth in serum- and protein-free media |
KR1020057006995A KR101186048B1 (ko) | 2002-10-23 | 2003-10-22 | 무단백질 배지에서 세포주의 수득 방법 및 수득된 세포주 |
DE60336415T DE60336415D1 (de) | 2002-10-23 | 2003-10-22 | Verfahren zur gewinnung einer rekombinanten säuger- myeloma zelllinie adaptiert an wachstum in serum- und proteinfreien medien |
AT03757654T ATE502103T1 (de) | 2002-10-23 | 2003-10-22 | Verfahren zur gewinnung einer rekombinanten säuger- myeloma zelllinie adaptiert an wachstum in serum- und proteinfreien medien |
AU2003273727A AU2003273727B2 (en) | 2002-10-23 | 2003-10-22 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
CN2003801036918A CN1714147B (zh) | 2002-10-23 | 2003-10-22 | 无蛋白培养基中获得细胞系的方法以及用此方法获得的细胞系 |
ES03757654T ES2360049T3 (es) | 2002-10-23 | 2003-10-22 | Procedimiento para obtener líneas celulares reombinantes de mieloma de mamiífero adaptadas al crecimiento en medios sin suero ni proteínas. |
EP03757654A EP1564285B1 (en) | 2002-10-23 | 2003-10-22 | Method of obtaining a recombinant mammalian myeloma cell lines adapted to growth in serum- and protein-free media |
NZ540171A NZ540171A (en) | 2002-10-23 | 2003-10-22 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
US10/532,269 US20060036082A1 (en) | 2002-10-23 | 2003-10-22 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
CA2503315A CA2503315C (en) | 2002-10-23 | 2003-10-22 | Procedure for obtaining cell lines in a protein-free media and cell line obtained by the method |
EA200500695A EA007465B1 (ru) | 2002-10-23 | 2003-10-22 | Процедура получения клеточных линий в безбелковых средах и клеточные линии, полученные этим способом |
DK03757654.3T DK1564285T3 (da) | 2002-10-23 | 2003-10-22 | Fremgangsmåde til opnåelse af en rekombinant pattedyr myelomacellelinje tilpasset at gro i serum- og proteinfri medier |
GEAP20038815A GEP20084444B (en) | 2002-10-23 | 2003-10-22 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
PCT/CU2003/000012 WO2004038010A1 (es) | 2002-10-23 | 2003-10-22 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
UY28037A UY28037A1 (es) | 2002-10-23 | 2003-10-23 | Metodo para la obtencion de lineas celulares en medio libre de proteina y lineas celulares obtenidas por este metodo |
EC2005005740A ECSP055740A (es) | 2002-10-23 | 2005-04-22 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método. |
TNP2005000116A TNSN05116A1 (en) | 2002-10-23 | 2005-04-22 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
LT2005053A LT5354B (lt) | 2002-10-23 | 2005-05-19 | Ląstelių linijų išskyrimo baltymų neturinčioje terpėje būdas ir šiuo būdu išskirtos ląstelių linijos |
LVP-05-61A LV13361B (en) | 2002-10-23 | 2005-05-23 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
HR20050760A HRP20050760A2 (en) | 2002-10-23 | 2005-09-02 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
HK06104515.2A HK1084414A1 (en) | 2002-10-23 | 2006-04-13 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
US12/759,208 US20100197011A1 (en) | 2002-10-23 | 2010-04-13 | Method of obtaining cell lines in a protein-free medium and cell lines thus obtained |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CU20020239A CU23097A1 (es) | 2002-10-23 | 2002-10-23 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
Publications (1)
Publication Number | Publication Date |
---|---|
CU23097A1 true CU23097A1 (es) | 2005-11-18 |
Family
ID=40134843
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CU20020239A CU23097A1 (es) | 2002-10-23 | 2002-10-23 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
Country Status (27)
Country | Link |
---|---|
US (2) | US20060036082A1 (es) |
EP (2) | EP2363418B1 (es) |
JP (1) | JP4674087B2 (es) |
KR (1) | KR101186048B1 (es) |
CN (1) | CN1714147B (es) |
AR (1) | AR041645A1 (es) |
AT (1) | ATE502103T1 (es) |
AU (1) | AU2003273727B2 (es) |
CA (1) | CA2503315C (es) |
CU (1) | CU23097A1 (es) |
DE (1) | DE60336415D1 (es) |
DK (1) | DK1564285T3 (es) |
EA (1) | EA007465B1 (es) |
EC (1) | ECSP055740A (es) |
ES (2) | ES2360049T3 (es) |
GE (1) | GEP20084444B (es) |
HK (1) | HK1084414A1 (es) |
HR (1) | HRP20050760A2 (es) |
LT (1) | LT5354B (es) |
LV (1) | LV13361B (es) |
MY (1) | MY144088A (es) |
NZ (1) | NZ540171A (es) |
PE (1) | PE20040552A1 (es) |
PT (1) | PT1564285E (es) |
TN (1) | TNSN05116A1 (es) |
UY (1) | UY28037A1 (es) |
WO (1) | WO2004038010A1 (es) |
Families Citing this family (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104673754B (zh) * | 2015-02-14 | 2018-03-02 | 百泰生物药业有限公司 | 重组骨髓瘤细胞克隆的筛选方法及由此获得的抗体分子 |
CN104651314B (zh) * | 2015-02-14 | 2018-06-19 | 百泰生物药业有限公司 | 获得高产稳定表达细胞克隆的方法及由此获得的抗体分子 |
Family Cites Families (19)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3694263A (en) * | 1970-10-23 | 1972-09-26 | Joseph J Korn Sr | Swimming pool cleaning methods and apparatus therefor |
CU22545A1 (es) * | 1994-11-18 | 1999-03-31 | Centro Inmunologia Molecular | Obtención de un anticuerpo quimérico y humanizado contra el receptor del factor de crecimiento epidérmico para uso diagnóstico y terapéutico |
US3886616A (en) * | 1972-12-06 | 1975-06-03 | Fay A Hayes | Hand propelled swimming pool cleaner |
US4275474A (en) * | 1979-04-30 | 1981-06-30 | Woodard Randle C | Vacuum head for swimming pool cleaning system |
US4240174A (en) * | 1979-07-30 | 1980-12-23 | Scott Jeffrey L | Self-contained mobile pool cleaning apparatus |
US4430214A (en) * | 1982-09-15 | 1984-02-07 | Baker Marvin E | Strainer mill for swimming pool pump intake |
AU2986484A (en) * | 1983-06-27 | 1985-01-03 | Monash University | Leptospira strains grown in protein free medium |
US4558479A (en) * | 1984-01-26 | 1985-12-17 | Alopex Industries, Inc. | Pool cleaner |
US4683599A (en) * | 1984-03-30 | 1987-08-04 | Fahet Nv | Automatic pool cleaner fitting |
US4581075A (en) * | 1985-03-15 | 1986-04-08 | Maxi-Sweep, Inc. | Self-propelled water borne pool cleaner |
WO1991005240A1 (en) | 1989-09-29 | 1991-04-18 | Atomic Energy Of Canada Limited | Infrared-based gas detector |
JP2696001B2 (ja) | 1991-04-15 | 1998-01-14 | 財団法人化学及血清療法研究所 | 組換え蛋白質産生用培地 |
EP0531562A1 (de) | 1991-09-11 | 1993-03-17 | Doerr, Hans-Wilhelm, Prof. Dr. med. | Kultivierung von Säugetierzellen |
ATE370235T1 (de) * | 1994-09-16 | 2007-09-15 | Cancer Res Fund Of Contra Cost | Rekombinantpeptide hergeleitet vom mc3 antikörper gegen ba46, verfahren zu deren verwendung und verfahren zur humanisierung von antikörperpeptiden |
ATE542891T1 (de) | 1994-11-10 | 2012-02-15 | Baxter Healthcare Sa | Verfahren zur herstellung von biologischen produkten in protein-freier kultur |
CA2211630A1 (en) | 1995-02-23 | 1996-08-29 | Quest International B.V. | Peptides for tissue and cell culture media |
AUPN442295A0 (en) | 1995-07-26 | 1995-08-17 | Commonwealth Scientific And Industrial Research Organisation | Regulated autocrine growth of mammalian cells |
CU22584A1 (es) * | 1995-11-17 | 1999-11-03 | Centro Inmunologia Molecular | Composiciones farmacéuticas que contienen un anticuerpo monoclonal que reconoce el antígeno de diferenciación leucocitario humano cd6 y sus usos para el diagnóstico y tratamiento de la psoriasis |
AT407255B (de) * | 1997-06-20 | 2001-02-26 | Immuno Ag | Rekombinanter zellklon mit erhöhter stabilität in serum- und proteinfreiem medium und verfahren zur gewinnung des stabilen zellklons |
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2002
- 2002-10-23 CU CU20020239A patent/CU23097A1/es unknown
-
2003
- 2003-10-10 PE PE2003001035A patent/PE20040552A1/es not_active Application Discontinuation
- 2003-10-16 AR ARP030103776A patent/AR041645A1/es active IP Right Grant
- 2003-10-17 MY MYPI20033959A patent/MY144088A/en unknown
- 2003-10-22 DE DE60336415T patent/DE60336415D1/de not_active Expired - Lifetime
- 2003-10-22 NZ NZ540171A patent/NZ540171A/en not_active IP Right Cessation
- 2003-10-22 ES ES03757654T patent/ES2360049T3/es not_active Expired - Lifetime
- 2003-10-22 ES ES11155076.0T patent/ES2624279T3/es not_active Expired - Lifetime
- 2003-10-22 AU AU2003273727A patent/AU2003273727B2/en not_active Expired
- 2003-10-22 AT AT03757654T patent/ATE502103T1/de active
- 2003-10-22 GE GEAP20038815A patent/GEP20084444B/en unknown
- 2003-10-22 CN CN2003801036918A patent/CN1714147B/zh not_active Expired - Lifetime
- 2003-10-22 US US10/532,269 patent/US20060036082A1/en not_active Abandoned
- 2003-10-22 EP EP11155076.0A patent/EP2363418B1/en not_active Expired - Lifetime
- 2003-10-22 WO PCT/CU2003/000012 patent/WO2004038010A1/es active IP Right Grant
- 2003-10-22 PT PT03757654T patent/PT1564285E/pt unknown
- 2003-10-22 EP EP03757654A patent/EP1564285B1/en not_active Expired - Lifetime
- 2003-10-22 EA EA200500695A patent/EA007465B1/ru not_active IP Right Cessation
- 2003-10-22 DK DK03757654.3T patent/DK1564285T3/da active
- 2003-10-22 KR KR1020057006995A patent/KR101186048B1/ko active IP Right Grant
- 2003-10-22 JP JP2004545689A patent/JP4674087B2/ja not_active Expired - Lifetime
- 2003-10-22 CA CA2503315A patent/CA2503315C/en not_active Expired - Lifetime
- 2003-10-23 UY UY28037A patent/UY28037A1/es not_active Application Discontinuation
-
2005
- 2005-04-22 EC EC2005005740A patent/ECSP055740A/es unknown
- 2005-04-22 TN TNP2005000116A patent/TNSN05116A1/en unknown
- 2005-05-19 LT LT2005053A patent/LT5354B/lt not_active IP Right Cessation
- 2005-05-23 LV LVP-05-61A patent/LV13361B/en unknown
- 2005-09-02 HR HR20050760A patent/HRP20050760A2/xx not_active Application Discontinuation
-
2006
- 2006-04-13 HK HK06104515.2A patent/HK1084414A1/xx not_active IP Right Cessation
-
2010
- 2010-04-13 US US12/759,208 patent/US20100197011A1/en not_active Abandoned
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