BR0315565A - Método para se obter uma linhagem celular de mamìfero adaptada para crescer em meio livre de soro e proteìna, linhagem celular de mamìfero, uso do método, anticorpos humanizados contra o receptor de egf hr3 ou seus fragmentos, contra o antìgeno cd6 t1ht ou seus fragmentos, e contra o antìgeno cd3 t3q ou seus fragmentos - Google Patents
Método para se obter uma linhagem celular de mamìfero adaptada para crescer em meio livre de soro e proteìna, linhagem celular de mamìfero, uso do método, anticorpos humanizados contra o receptor de egf hr3 ou seus fragmentos, contra o antìgeno cd6 t1ht ou seus fragmentos, e contra o antìgeno cd3 t3q ou seus fragmentosInfo
- Publication number
- BR0315565A BR0315565A BR0315565-0A BR0315565A BR0315565A BR 0315565 A BR0315565 A BR 0315565A BR 0315565 A BR0315565 A BR 0315565A BR 0315565 A BR0315565 A BR 0315565A
- Authority
- BR
- Brazil
- Prior art keywords
- fragments
- cell line
- mammalian cell
- antigen
- free medium
- Prior art date
Links
- 239000012634 fragment Substances 0.000 title abstract 8
- 102000004169 proteins and genes Human genes 0.000 title abstract 7
- 108090000623 proteins and genes Proteins 0.000 title abstract 7
- 210000004962 mammalian cell Anatomy 0.000 title abstract 6
- 239000000427 antigen Substances 0.000 title abstract 3
- 102000036639 antigens Human genes 0.000 title abstract 3
- 108091007433 antigens Proteins 0.000 title abstract 3
- 210000002966 serum Anatomy 0.000 title abstract 3
- 210000004027 cell Anatomy 0.000 abstract 2
- 102000001301 EGF receptor Human genes 0.000 abstract 1
- 108060006698 EGF receptor Proteins 0.000 abstract 1
- 239000005862 Whey Substances 0.000 abstract 1
- 102000007544 Whey Proteins Human genes 0.000 abstract 1
- 108010046377 Whey Proteins Proteins 0.000 abstract 1
- 230000006978 adaptation Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2803—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily
- C07K16/2809—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the immunoglobulin superfamily against the T-cell receptor (TcR)-CD3 complex
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2863—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against receptors for growth factors, growth regulators
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2896—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against molecules with a "CD"-designation, not provided for elsewhere
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0693—Tumour cells; Cancer cells
- C12N5/0694—Cells of blood, e.g. leukemia cells, myeloma cells
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2500/00—Specific components of cell culture medium
- C12N2500/90—Serum-free medium, which may still contain naturally-sourced components
- C12N2500/95—Protein-free medium and culture conditions
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2510/00—Genetically modified cells
- C12N2510/02—Cells for production
Abstract
"MéTODO PARA SE OBTER UMA LINHAGEM CELULAR DE MAMìFERO ADAPTADA PARA CRESCER EM MEIO LIVRE DE SORO E PROTEìNA, LINHAGEM CELULAR DE MAMìFERO, USO DO MéTODO, ANTICORPOS HUMANIZADOS CONTRA O RECEPTOR DE EGF HR3 OU SEUS FRAGMENTOS, CONTRA O ANTìGENO CD6 T1HT OU SEUS FRAGMENTOS, E CONTRA O ANTìGENO CD3 T3Q OU SEUS FRAGMENTOS". A presente invenção diz respeito a um método de obtenção de células de mamífero adaptadas a crescer em meio livre de soro e de proteínas, o qual inclui um processo em duas etapas de adaptação em que existe um intervalo crítico de concentração de proteína em que as células devem adquirir a capacidade de sobreviver em meio livre de proteínas. O intervalo crítico da concentração da proteína é especifico para cada linhagem celular. A presente invenção também diz respeito a linhagens celulares de mamíferos que crescem de uma maneira estável em um meio livre de soro e proteínas por pelo menos 40 gerações. A invenção ainda diz respeito a clones que expressam os anticorpos humanizados anti-receptores de EGF hR~ 3~ e anti-CD6 T1hT e o anticorpo quimérico anti-CD~ 3~ T~ 3~Q, assim como os fragmentos dos referidos anticorpos.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CU20020239A CU23097A1 (es) | 2002-10-23 | 2002-10-23 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
| CU239/2002 | 2002-10-23 | ||
| PCT/CU2003/000012 WO2004038010A1 (es) | 2002-10-23 | 2003-10-22 | Método para la obtención de líneas celulares en medio libre de proteína y líneas celulares obtenidas por este método |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| BR0315565A true BR0315565A (pt) | 2005-08-23 |
| BR0315565B1 BR0315565B1 (pt) | 2022-01-25 |
| BRPI0315565B1 BRPI0315565B1 (pt) | 2022-01-25 |
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