BR112016021572A2 - Métodos para preparar um animal não humano geneticamente modificado e para produzir proteínas de ligação, hibridoma, ácido nucleico, célula, e, proteína de ligação - Google Patents

Métodos para preparar um animal não humano geneticamente modificado e para produzir proteínas de ligação, hibridoma, ácido nucleico, célula, e, proteína de ligação

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Publication number
BR112016021572A2
BR112016021572A2 BR112016021572A BR112016021572A BR112016021572A2 BR 112016021572 A2 BR112016021572 A2 BR 112016021572A2 BR 112016021572 A BR112016021572 A BR 112016021572A BR 112016021572 A BR112016021572 A BR 112016021572A BR 112016021572 A2 BR112016021572 A2 BR 112016021572A2
Authority
BR
Brazil
Prior art keywords
genetically modified
light chain
modified non
binding proteins
preparing
Prior art date
Application number
BR112016021572A
Other languages
English (en)
Inventor
Macdonald Lynn
J Murphy Andrew
Gurer Cagan
Mcwhirter John
Original Assignee
Regeneron Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Regeneron Pharma filed Critical Regeneron Pharma
Publication of BR112016021572A2 publication Critical patent/BR112016021572A2/pt

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Classifications

    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K67/00—Rearing or breeding animals, not otherwise provided for; New breeds of animals
    • A01K67/027—New breeds of vertebrates
    • A01K67/0275—Genetically modified vertebrates, e.g. transgenic
    • A01K67/0278—Humanized animals, e.g. knockin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2207/00—Modified animals
    • A01K2207/15—Humanized animals
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/072—Animals genetically altered by homologous recombination maintaining or altering function, i.e. knock in
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/15—Animals comprising multiple alterations of the genome, by transgenesis or homologous recombination, e.g. obtained by cross-breeding
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2227/00—Animals characterised by species
    • A01K2227/10—Mammal
    • A01K2227/105—Murine
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; CARE OF BIRDS, FISHES, INSECTS; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2267/00—Animals characterised by purpose
    • A01K2267/01—Animal expressing industrially exogenous proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/515—Complete light chain, i.e. VL + CL
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/52—Constant or Fc region; Isotype
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/50—Immunoglobulins specific features characterized by immunoglobulin fragments
    • C07K2317/56—Immunoglobulins specific features characterized by immunoglobulin fragments variable (Fv) region, i.e. VH and/or VL
    • C07K2317/569—Single domain, e.g. dAb, sdAb, VHH, VNAR or nanobody®
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/60—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments
    • C07K2317/64—Immunoglobulins specific features characterized by non-natural combinations of immunoglobulin fragments comprising a combination of variable region and constant region components
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/8509—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic
    • C12N2015/8518—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells for producing genetically modified animals, e.g. transgenic expressing industrially exogenous proteins, e.g. for pharmaceutical use, human insulin, blood factors, immunoglobulins, pseudoparticles

Abstract

ANIMAL NÃO HUMANO GENETICAMENTE MODIFICADO, CAMUNDONGO GENETICAMENTE MODIFICADO, MÉTODOS PARA PREPARAR UM ANIMAL NÃO HUMANO GENETICAMENTE MODIFICADO E PARA PRODUZIR PROTEÍNAS DE LIGAÇÃO, HIBRIDOMA, ÁCIDO NUCLEICO, CÉLULA, E, PROTEÍNA DE LIGAÇÃO. Animais não humanos geneticamente modificados e métodos e composições para preparar e usar os mesmos são fornecidos, em que a modificação genética compreende (a) uma deleção num gene da região constante de imunoglobulina CH1 (opcionalmente uma deleção em uma região de dobradiça) de uma sequência de gene da região constante de cadeia pesada e (b) substituição de um ou todos os segmentos de gene VH, DH e JH endógeno com pelo menos um segmento de gene variável de cadeia leve (VL) não rearranjado e pelo menos um segmento de gene de junção de cadeia leve (JL) não rearranjado capazes de se recombinar para formar uma sequência de nucleotídeo de região variável de cadeia leve (VL/JL) rearranjada operavelmente ligada à sequência de gene da região constante de cadeia pesada compreendendo uma deleção no gene CH1 e/ou inserção de uma cadeia leve rearranjada única geneticamente projetada, em que o camundongo é capaz de expressar uma IgM funcional, proteínas de ligação a antígeno de domínio único, por exemplo, proteínas de ligação de domínio único VL, e uma cadeia leve rearranjada geneticamente projetada.
BR112016021572A 2014-03-21 2015-03-20 Métodos para preparar um animal não humano geneticamente modificado e para produzir proteínas de ligação, hibridoma, ácido nucleico, célula, e, proteína de ligação BR112016021572A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201461968986P 2014-03-21 2014-03-21
US201461968905P 2014-03-21 2014-03-21
PCT/US2015/021892 WO2015143414A2 (en) 2014-03-21 2015-03-20 Non-human animals that make single domain binding proteins

Publications (1)

Publication Number Publication Date
BR112016021572A2 true BR112016021572A2 (pt) 2017-10-03

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BR112016021572A BR112016021572A2 (pt) 2014-03-21 2015-03-20 Métodos para preparar um animal não humano geneticamente modificado e para produzir proteínas de ligação, hibridoma, ácido nucleico, célula, e, proteína de ligação

Country Status (14)

Country Link
US (2) US10881085B2 (pt)
EP (2) EP3119194B1 (pt)
JP (1) JP6636498B2 (pt)
KR (4) KR102601491B1 (pt)
CN (1) CN106255410B (pt)
AU (1) AU2015230938C1 (pt)
BR (1) BR112016021572A2 (pt)
CA (2) CA2942697A1 (pt)
ES (1) ES2872475T3 (pt)
IL (1) IL247524A0 (pt)
MX (1) MX2016012274A (pt)
RU (1) RU2016141307A (pt)
SG (2) SG11201607203XA (pt)
WO (1) WO2015143414A2 (pt)

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US10881085B2 (en) 2021-01-05
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CN106255410A (zh) 2016-12-21
KR20220111726A (ko) 2022-08-09
IL247524A0 (en) 2016-11-30
EP3895528A1 (en) 2021-10-20
US20210084872A1 (en) 2021-03-25
EP3119194A2 (en) 2017-01-25
CA3124228A1 (en) 2015-09-24
SG11201607203XA (en) 2016-09-29
KR20210088756A (ko) 2021-07-14
AU2015230938B2 (en) 2021-04-01
CN106255410B (zh) 2020-01-10
ES2872475T3 (es) 2021-11-02
KR102276752B1 (ko) 2021-07-13
JP6636498B2 (ja) 2020-01-29
WO2015143414A2 (en) 2015-09-24
RU2016141307A3 (pt) 2018-10-24
US20150289489A1 (en) 2015-10-15
MX2016012274A (es) 2017-05-23
EP3119194B1 (en) 2021-04-28
KR102601491B1 (ko) 2023-11-13
WO2015143414A3 (en) 2015-12-23
SG10201808225TA (en) 2018-10-30
CA2942697A1 (en) 2015-09-24

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