ES2045081T3 - Amplificacion estable de genes en adn cromosonal procariotica. - Google Patents
Amplificacion estable de genes en adn cromosonal procariotica.Info
- Publication number
- ES2045081T3 ES2045081T3 ES88200376T ES88200376T ES2045081T3 ES 2045081 T3 ES2045081 T3 ES 2045081T3 ES 88200376 T ES88200376 T ES 88200376T ES 88200376 T ES88200376 T ES 88200376T ES 2045081 T3 ES2045081 T3 ES 2045081T3
- Authority
- ES
- Spain
- Prior art keywords
- strains
- polypeptide
- transformed
- hosting
- bacillus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/67—General methods for enhancing the expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
LOS HOSPEDADORES PROCARITAS TRANSFORMADOS SE PROPORCIONAN INCLUYENDO DOS O MAS COPIAS DE LA SECUENCIA DE ADN ESTABLE MANTENIDA EN SU CROMOSOMA, DICHA SECUENCIA COMPRENDE UN GEN QUE CODIFICA UN POLIPEPTIDO DE INTERES, EN DONDE DICHAS COPIAS SE SEPARAN POR SECUENCIAS DE ADN CROMOSOMAL ENDOGENO. LOS METODOS TAMBIEN PROPORCIONAN LA PRODUCCION DE DICHAS CEPAS HOSPEDADORAS TRANSFORMADAS. DICHAS CEPAS HOSPEDADORAS TRANSFORMADAS SON CAPACES DE INCREMENTAR LA PRODUCCION DEL POLIPEPTIDO DE INTERES EN COMPARACION CON LAS CEPAS HOSPEDADORAS QUE YA PRODUCEN DICHO POLIPEPTIDO. LAS CEPAS HOSPEDADORAS PREFERIDAS SON BACILLUS ESPECIE NUEVA PB92 QUE PRODUCE UN ENZIMA PROTEOLITICO MUY ALCALINO Y BACILLUS LICHENIFORMS T5 QUE PRODUCE UNA ALFA-AMILASA TERMOESTABLE Y MUTANTES Y VARIANTES DE DICHAS CEPAS. EL GEN CODIFICADOR DE POLIPEPTIDOS PREFERIDO ES EL GEN CODIFICADOR DE LA PROTEASA ORIGINADA POR BACILLUS PB92 Y GEN CODIFICADOR DE LA ALFA-AMILASA ORIGINADA POR BACILLUS LICHENIFORMIS CEPA T5.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP87200356 | 1987-02-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
ES2045081T3 true ES2045081T3 (es) | 1994-01-16 |
Family
ID=8197582
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ES88200376T Expired - Lifetime ES2045081T3 (es) | 1987-02-27 | 1988-02-29 | Amplificacion estable de genes en adn cromosonal procariotica. |
Country Status (20)
Country | Link |
---|---|
US (2) | US5733723A (es) |
EP (1) | EP0284126B1 (es) |
JP (1) | JP2637532B2 (es) |
KR (1) | KR970000188B1 (es) |
CN (1) | CN1049247C (es) |
AU (1) | AU620326B2 (es) |
BG (1) | BG60920B2 (es) |
BR (1) | BR8805646A (es) |
CA (1) | CA1327175C (es) |
DE (1) | DE3883041T2 (es) |
ES (1) | ES2045081T3 (es) |
FI (1) | FI104326B1 (es) |
HU (1) | HUT50877A (es) |
IE (1) | IE61743B1 (es) |
LT (1) | LT4001B (es) |
LV (1) | LV10791B (es) |
NO (1) | NO300382B1 (es) |
PT (1) | PT86863B (es) |
RU (1) | RU2091487C1 (es) |
WO (1) | WO1988006623A1 (es) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
ES2134186T3 (es) * | 1987-02-27 | 1999-10-01 | Genencor Int | Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. |
JP2637532B2 (ja) * | 1987-02-27 | 1997-08-06 | ギスト ブロカデス ナームローゼ フェンノートチャップ | 原核微生物の染色体dnaにおける安定した遺伝子増幅 |
US4914031A (en) * | 1987-04-10 | 1990-04-03 | Amgen, Inc. | Subtilisin analogs |
US6287841B1 (en) | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
ES2095233T3 (es) * | 1989-08-11 | 1997-02-16 | Gist Brocades Nv | Produccion eficaz de proteasa mutante. |
US5695976A (en) * | 1989-12-18 | 1997-12-09 | Novo Nordisk A/S | Stable integration of DNA in bacterial genomes |
JP3046344B2 (ja) * | 1990-10-24 | 2000-05-29 | 塩野義製薬株式会社 | 新規プロテアーゼ |
WO1993010248A1 (en) * | 1991-11-14 | 1993-05-27 | Novo Nordisk A/S | A PROCESS FOR EXPRESSING GENES IN $i(BACILLUS LICHENIFORMIS) |
DE4216246A1 (de) * | 1992-05-16 | 1993-11-18 | Solvay Enzymes Gmbh & Co Kg | Einfaches Verfahren zur stabilen chromosomalen Genamplifikation |
US5395763A (en) * | 1992-06-24 | 1995-03-07 | Monsanto Company | Chromosomal expression vector |
DK153992D0 (da) * | 1992-12-22 | 1992-12-22 | Novo Nordisk As | Metode |
US5861273A (en) * | 1993-12-21 | 1999-01-19 | Celtrix Phamraceuticals, Inc. | Chromosomal expression of heterologous genes in bacterial cells |
US6723550B1 (en) | 1997-07-15 | 2004-04-20 | Genencor International, Inc. | Proteases from gram-positive organisms |
GB9724627D0 (en) | 1997-11-20 | 1998-01-21 | Genencor Int Bv | Gram positive microorganism formate pathway |
US6465186B1 (en) | 1997-12-30 | 2002-10-15 | Genecor International, Inc. | Proteases from gram positive organisms |
US6599731B1 (en) | 1997-12-30 | 2003-07-29 | Genencor International, Inc. | Proteases from gram positive organisms |
GB9727470D0 (en) | 1997-12-30 | 1998-02-25 | Genencor Int Bv | Proteases from gram positive organisms |
US6528255B1 (en) | 1997-12-30 | 2003-03-04 | Genencor International, Inc. | Proteases from gram positive organisms |
EP1062318B1 (en) * | 1998-02-12 | 2004-04-28 | Novozymes A/S | A prokaryotic cell comprising two copies of a gene transcribed in different directions |
US6100063A (en) * | 1998-02-12 | 2000-08-08 | Novo Nordisk A/S | Procaryotic cell comprising at least two copies of a gene |
US6156514A (en) * | 1998-12-03 | 2000-12-05 | Sunol Molecular Corporation | Methods for making recombinant cells |
US6544792B1 (en) | 1999-12-21 | 2003-04-08 | Genencor International, Inc. | Production of secreted polypeptides |
JP2004501651A (ja) * | 2000-06-23 | 2004-01-22 | ノボザイムス アクティーゼルスカブ | 遺伝子の安定した染色体多コピー組み込みのための方法 |
BRPI0416797A (pt) | 2003-11-19 | 2007-04-17 | Genencor Int | serina proteases, ácidos nucléicos codificando enzimas de serina e vetores e células hospedeiras incorporando as mesmas |
US7985569B2 (en) | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
US20050227356A1 (en) * | 2004-04-07 | 2005-10-13 | Lessard Philip A | Novel compositions and methods for genetic manipulation of Rhodococcus bacteria |
WO2006065272A2 (en) * | 2004-05-21 | 2006-06-22 | Idaho Research Foundation, Inc. | Methods for altering acetic acid production and enhancing cell death in bacteria |
EP1766002B1 (en) * | 2004-06-21 | 2015-11-18 | Novozymes A/S | Stably maintained multiple copies of at least two orf in the same orientation |
US20080085535A1 (en) | 2004-10-22 | 2008-04-10 | Novozymes A/S | Stable Genomic Integration of Multiple Polynucleotide Copies |
DE102007021001A1 (de) | 2007-05-04 | 2008-11-06 | Ab Enzymes Gmbh | Expressionssystem zur antibiotikafreien Produktion von Polypeptiden |
CN103290048B (zh) * | 2013-06-27 | 2015-07-15 | 天津科技大学 | 一种适用于芽孢杆菌属同源重组的质粒及工程菌 |
US11046732B2 (en) | 2015-12-11 | 2021-06-29 | Wacker Chemie Ag | Microorganism strain and method for antibiotic-free, fermentative preparation of low molecular weight substances and proteins |
CN114032189B (zh) * | 2021-05-10 | 2024-02-06 | 盐城师范学院 | 一种降解木质素的融合子菌株r3及其应用 |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US30602A (en) * | 1860-11-06 | Improvement in revolver fire-arms | ||
GB1519148A (en) * | 1974-11-19 | 1978-07-26 | Gist Brocades Nv | Compositions of matter |
NO159863C (no) * | 1980-01-07 | 1989-02-15 | Univ Rochester | Fremgangsm te for fremstilling og seleksjon av en rant bakteriofag som inneholder et genetisk fragment og koder for alfa-amylase, egnet for bruk i heterolog transformering av en bacillus-verts-mikroorganisme. |
IL61982A (en) * | 1980-02-15 | 1984-01-31 | Cpc International Inc | Genetically engineered microorganisms for massive production of amyloytic enzymes and process for preparing same using the corresponding recombinant dnas containing amylase coding genes |
EP0074553A3 (en) * | 1981-09-11 | 1985-01-09 | The University Of Rochester | Method of increasing the yield of a product by altering a microorganism |
JPS59205983A (ja) * | 1983-04-28 | 1984-11-21 | ジエネツクス・コ−ポレイシヨン | 異種遺伝子を原核微生物で発現させる方法 |
US4617266A (en) * | 1983-04-28 | 1986-10-14 | Genex Corporation | Production of Protein A |
NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
EP0134048B2 (en) * | 1983-07-06 | 1996-08-14 | Gist-Brocades N.V. | Molecular cloning and expression in industrial microorganism species |
FR2563533B1 (fr) * | 1984-04-27 | 1986-08-22 | Centre Nat Rech Scient | Procede d'amplification de l'expression d'un gene determine chez bacillus subtilis et souches obtenues |
US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
FR2582316B1 (fr) * | 1985-05-22 | 1988-12-02 | Centre Nat Rech Scient | Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase |
EP0254735B2 (en) * | 1986-01-15 | 1998-06-17 | Amgen Inc. | METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS |
JP2637532B2 (ja) * | 1987-02-27 | 1997-08-06 | ギスト ブロカデス ナームローゼ フェンノートチャップ | 原核微生物の染色体dnaにおける安定した遺伝子増幅 |
-
1988
- 1988-02-26 JP JP63502471A patent/JP2637532B2/ja not_active Expired - Lifetime
- 1988-02-26 BR BR888805646A patent/BR8805646A/pt not_active Application Discontinuation
- 1988-02-26 RU SU884356795A patent/RU2091487C1/ru active
- 1988-02-26 HU HU881833A patent/HUT50877A/hu unknown
- 1988-02-26 WO PCT/NL1988/000006 patent/WO1988006623A1/en active IP Right Grant
- 1988-02-26 AU AU13981/88A patent/AU620326B2/en not_active Expired
- 1988-02-26 PT PT86863A patent/PT86863B/pt not_active IP Right Cessation
- 1988-02-27 CN CN88101680A patent/CN1049247C/zh not_active Expired - Lifetime
- 1988-02-29 CA CA000560122A patent/CA1327175C/en not_active Expired - Lifetime
- 1988-02-29 EP EP88200376A patent/EP0284126B1/en not_active Revoked
- 1988-02-29 DE DE88200376T patent/DE3883041T2/de not_active Revoked
- 1988-02-29 ES ES88200376T patent/ES2045081T3/es not_active Expired - Lifetime
- 1988-02-29 IE IE55888A patent/IE61743B1/en not_active IP Right Cessation
- 1988-10-05 NO NO884422A patent/NO300382B1/no unknown
- 1988-10-21 KR KR88701325A patent/KR970000188B1/ko active IP Right Grant
- 1988-10-24 FI FI884903A patent/FI104326B1/fi active IP Right Grant
- 1988-10-25 BG BG85832A patent/BG60920B2/bg unknown
-
1993
- 1993-12-08 LV LVP-93-1313A patent/LV10791B/en unknown
-
1994
- 1994-01-28 LT LTIP1826A patent/LT4001B/lt not_active IP Right Cessation
- 1994-08-24 US US08/295,082 patent/US5733723A/en not_active Expired - Lifetime
-
1998
- 1998-03-27 US US09/049,867 patent/US6124097A/en not_active Expired - Fee Related
Also Published As
Publication number | Publication date |
---|---|
EP0284126B1 (en) | 1993-08-11 |
JP2637532B2 (ja) | 1997-08-06 |
KR970000188B1 (en) | 1997-01-06 |
JPH01502398A (ja) | 1989-08-24 |
WO1988006623A1 (en) | 1988-09-07 |
DE3883041T2 (de) | 1994-01-20 |
EP0284126A1 (en) | 1988-09-28 |
KR890700664A (ko) | 1989-04-26 |
CA1327175C (en) | 1994-02-22 |
RU2091487C1 (ru) | 1997-09-27 |
IE61743B1 (en) | 1994-11-30 |
NO884422D0 (no) | 1988-10-05 |
DE3883041D1 (de) | 1993-09-16 |
LV10791B (en) | 1995-12-20 |
US6124097A (en) | 2000-09-26 |
PT86863B (pt) | 1992-05-29 |
FI884903A (fi) | 1988-10-24 |
AU620326B2 (en) | 1992-02-20 |
BR8805646A (pt) | 1989-10-17 |
LT4001B (en) | 1996-06-25 |
IE880558L (en) | 1988-08-27 |
FI104326B (fi) | 1999-12-31 |
PT86863A (pt) | 1988-03-01 |
HUT50877A (en) | 1990-03-28 |
CN1030787A (zh) | 1989-02-01 |
FI884903A0 (fi) | 1988-10-24 |
US5733723A (en) | 1998-03-31 |
NO300382B1 (no) | 1997-05-20 |
AU1398188A (en) | 1988-09-26 |
BG60920B2 (bg) | 1996-06-28 |
NO884422L (no) | 1988-10-05 |
FI104326B1 (fi) | 1999-12-31 |
LV10791A (lv) | 1995-08-20 |
LTIP1826A (en) | 1995-08-25 |
CN1049247C (zh) | 2000-02-09 |
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