ES2134186T3 - Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. - Google Patents

Clonaje molecular y expresion de genes que codifican enzimas proteoliticas.

Info

Publication number
ES2134186T3
ES2134186T3 ES88200377T ES88200377T ES2134186T3 ES 2134186 T3 ES2134186 T3 ES 2134186T3 ES 88200377 T ES88200377 T ES 88200377T ES 88200377 T ES88200377 T ES 88200377T ES 2134186 T3 ES2134186 T3 ES 2134186T3
Authority
ES
Spain
Prior art keywords
bacillus
serine protease
expression
molecular cloning
proteolytic enzymes
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES88200377T
Other languages
English (en)
Inventor
Eekelen Christian Albertus Van
Der Laan Johannes Cornelis Van
Leonardus Johannes S Mulleners
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Application granted granted Critical
Publication of ES2134186T3 publication Critical patent/ES2134186T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)

Abstract

LA INVENCION PROPORCIONA NUEVOS METODOS Y NUEVAS CEPAS INDUSTRIALES DE BACILLUS PARA PRODUCCION INCREMENTADA DE PROTEASA SERINA. SE INTRODUCEN BLOQUES ESTRUCTURALES DE PLASMIDOS QUE CONTIENEN EL GEN DE PROTEASA SERINA, EN UN HUESPED COMPATIBLE, GENERALMENTE UN BACILLUS QUE YA ES SUPERPRODUCTOR DE PROTEASA SERINA. LAS CELULAS HUESPED PREFERIDAS SON LAS DE BACILLUS NOVO SP.PB92 Y EL GEN DE PROTEASA SERINA PREFERIDA TAMBIEN SE ORIGINA A PARTIR DE BACILLUS PB92. SE PUEDE CONSEGUIR LA INTEGRACION Y MANTENIMIENTO DEL PLASMIDO EN EL CROMOSOMA DE LA CELULA HUESPED, INCLUYENDO UN ORIGEN DE REPLICACION SENSIBLE A LA TEMPERATURA EN EL BLOQUE ESTRUCTURAL DEL PLASMIDO Y CRECIMIENTO EN CONDICIONES SELECTIVAS.
ES88200377T 1987-02-27 1988-02-29 Clonaje molecular y expresion de genes que codifican enzimas proteoliticas. Expired - Lifetime ES2134186T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
EP88200377A EP0283075B1 (en) 1987-02-27 1988-02-29 Molecular cloning and expression of genes encoding proteolytic enzymes

Publications (1)

Publication Number Publication Date
ES2134186T3 true ES2134186T3 (es) 1999-10-01

Family

ID=26108983

Family Applications (2)

Application Number Title Priority Date Filing Date
ES91203297T Expired - Lifetime ES2135386T3 (es) 1987-02-27 1988-02-29 Transformacion de cepas de bacillus alcalofilas.
ES88200377T Expired - Lifetime ES2134186T3 (es) 1987-02-27 1988-02-29 Clonaje molecular y expresion de genes que codifican enzimas proteoliticas.

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES91203297T Expired - Lifetime ES2135386T3 (es) 1987-02-27 1988-02-29 Transformacion de cepas de bacillus alcalofilas.

Country Status (2)

Country Link
EP (3) EP0896062A3 (es)
ES (2) ES2135386T3 (es)

Families Citing this family (70)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5185258A (en) 1984-05-29 1993-02-09 Genencor International, Inc. Subtilisin mutants
BR8805647A (pt) * 1987-02-27 1989-10-31 Gist Brocades Nv Processo para clonagem molecular e expressao de genes que codificam para enzimas proteoliticas
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
US6287841B1 (en) 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用
JPH03505976A (ja) * 1989-05-17 1991-12-26 アムジエン・インコーポレーテツド 多重突然変異スブチリシン
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK0414297T3 (da) * 1989-08-11 1997-07-07 Genencor Int Effektiv fremstilling af mutantproteaser
JPH07108228B2 (ja) * 1990-10-15 1995-11-22 味の素株式会社 温度感受性プラスミド
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
DE10121463A1 (de) * 2001-05-02 2003-02-27 Henkel Kgaa Neue Alkalische Protease-Varianten und Wasch- und Reinigungsmittel enthaltend diese neuen Alkalischen Protease-Varianten
DE10162728A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14393) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10162727A1 (de) 2001-12-20 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus gibsonii (DSM 14391) und Wasch-und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10163884A1 (de) 2001-12-22 2003-07-10 Henkel Kgaa Neue Alkalische Protease aus Bacillus sp. (DSM 14392) und Wasch- und Reinigungsmittel enthaltend diese neue Alkalische Protease
DE10360805A1 (de) 2003-12-23 2005-07-28 Henkel Kgaa Neue Alkalische Protease und Wasch- und Reinigungsmittel, enthaltend diese neue Alkalische Protease
DE102004019751A1 (de) 2004-04-23 2005-11-17 Henkel Kgaa Neue Alkalische Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Alkalischen Proteasen
DE102007032111B4 (de) 2007-07-09 2017-07-20 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102007036756A1 (de) 2007-08-03 2009-02-05 Henkel Ag & Co. Kgaa Neue Proteasen und Wasch- und Reinigungsmittel, enthaltend diese neuen Proteasen
DE102007038031A1 (de) 2007-08-10 2009-06-04 Henkel Ag & Co. Kgaa Mittel enthaltend Proteasen
DE102007044415A1 (de) 2007-09-17 2009-03-19 Henkel Ag & Co. Kgaa Leistungsverbesserte Proteasen und Wasch- und Reinigungsmittel enthaltend diese Proteasen
DE102014223296A1 (de) 2014-11-14 2016-05-19 Henkel Ag & Co. Kgaa Wasch- und Reinigungsmittel, enthaltend mindestens zwei Proteasen
DE102014223969A1 (de) 2014-11-25 2016-05-25 Henkel Ag & Co. Kgaa Verwendung von Molkenproteinisolat in enzymhaltigen Wasch- oder Reinigungsmitteln zur Erhöhung der Stabilität von Enzymen
DE102014226251A1 (de) 2014-12-17 2016-06-23 Henkel Ag & Co. Kgaa Verwendung anorganischer Oxide, Hydroxide oder Oxidhydroxide in enzymhaltigen Wasch- oder Reinigungsmitteln zur Erhöhung der Stabilität von Enzymen
DE102015217816A1 (de) 2015-09-17 2017-03-23 Henkel Ag & Co. Kgaa Verwendung hochkonzentrierter Enzymgranulate zur Erhöhung der Lagerstabilität von Enzymen
DE102015223269A1 (de) 2015-11-25 2017-06-01 Henkel Ag & Co. Kgaa Verwendung von Polyoxyalkylenaminen in enzymhaltigen Wasch- oder Reinigungsmitteln zur Erhöhung der Stabilität von Enzymen
EP3205392A1 (en) 2016-02-12 2017-08-16 Basf Se Microcapsules and process for preparation of microcapsules
EP3205393A1 (en) 2016-02-12 2017-08-16 Basf Se Process for preparation of microcapsules
WO2017182295A1 (en) 2016-04-18 2017-10-26 Basf Se Liquid cleaning compositions
RU2019106488A (ru) 2016-08-08 2020-09-14 Басф Се Жидкая композиция для стирки
KR20190086540A (ko) 2016-12-01 2019-07-22 바스프 에스이 조성물 중 효소의 안정화
KR20200088433A (ko) 2017-11-29 2020-07-22 바스프 에스이 보관 안정한 효소 제제, 이것의 제조 및 용도
EP3784779A1 (en) 2018-04-26 2021-03-03 Basf Se Lipase enzymes
US20210115422A1 (en) 2018-05-03 2021-04-22 Basf Se Amylase enzymes
WO2019238761A1 (en) 2018-06-15 2019-12-19 Basf Se Water soluble multilayer films containing wash active chemicals and enzymes
WO2020030623A1 (en) 2018-08-10 2020-02-13 Basf Se Packaging unit comprising a detergent composition containing an enzyme and at least one chelating agent
WO2020069914A1 (en) 2018-10-05 2020-04-09 Basf Se Compounds stabilizing amylases in liquids
US20210395650A1 (en) 2018-10-05 2021-12-23 Basf Se Compounds stabilizing hydrolases in liquids
EP3677676A1 (en) 2019-01-03 2020-07-08 Basf Se Compounds stabilizing amylases in liquids
CN112805376A (zh) 2018-10-05 2021-05-14 巴斯夫欧洲公司 在液体中稳定水解酶的化合物
WO2020104231A1 (en) 2018-11-19 2020-05-28 Basf Se Powders and granules containing a chelating agent and an enzyme
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
MX2021010110A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de medio definido y alimentacion de magnesio.
WO2020229480A1 (en) 2019-05-14 2020-11-19 Basf Se Compounds stabilizing hydrolases in liquids
EP3983425A1 (en) 2019-06-13 2022-04-20 Basf Se Method of recovering a protein from fermentation broth using a divalent cation
BR112021023457A2 (pt) 2019-07-01 2022-01-25 Basf Se Composto, uso do composto, composição, e, métodos para preparar a composição e um composto
US20220340865A1 (en) 2019-07-02 2022-10-27 Basf Se Method for preparing a fermentation medium
PL3959326T3 (pl) 2019-07-05 2023-10-09 Basf Se Przemysłowy sposób fermentacji dla komórek bakteryjnych z wykorzystaniem kultury wstępnej okresowej z zasilaniem
BR112022006082A2 (pt) 2019-10-18 2022-06-21 Basf Se Preparação enzimática, formulação de detergente, e, uso de pelo menos um diol
CN113891931A (zh) 2019-11-29 2022-01-04 巴斯夫欧洲公司 组合物和可以用于该类组合物的聚合物
WO2021115912A1 (en) 2019-12-09 2021-06-17 Basf Se Formulations comprising a hydrophobically modified polyethyleneimine and one or more enzymes
MX2022009968A (es) 2020-02-14 2022-09-19 Basf Se Variantes de mananasa.
BR112022025593A2 (pt) 2020-06-18 2023-01-03 Basf Se Composições e seu uso
JP2023537833A (ja) 2020-07-09 2023-09-06 ビーエーエスエフ ソシエタス・ヨーロピア 組成物及びその用途
WO2022008732A1 (en) 2020-07-10 2022-01-13 Basf Se Enhancing the activity of antimicrobial preservatives
BR112023005106A2 (pt) 2020-09-22 2023-04-18 Basf Se Composição líquida, formulação de detergente líquida, e, usos de pelo menos um diol (exceto 1,2-proanodiol) e das formulações de detergente
WO2022083949A1 (en) 2020-10-20 2022-04-28 Basf Se Compositions and their use
EP4015629A1 (en) 2020-12-18 2022-06-22 Basf Se Polymer mixtures for increasing stability and performance of hydrolase-containing detergents
WO2022178432A1 (en) 2021-02-22 2022-08-25 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
CN118202030A (zh) 2021-10-13 2024-06-14 巴斯夫欧洲公司 包含聚合物的组合物、聚合物及其用途
WO2023066741A1 (en) 2021-10-20 2023-04-27 Basf Se Phosphate-free composition and methods for their manufacture and use
CN118284680A (zh) 2021-11-22 2024-07-02 巴斯夫欧洲公司 包含聚合物的组合物、聚合物及其用途
WO2023088761A1 (en) 2021-11-22 2023-05-25 Basf Se Compositions comprising polymers, polymers, and their use
WO2023088777A1 (en) 2021-11-22 2023-05-25 Basf Se Compositions comprising polymers, polymers, and their use
WO2023110599A2 (en) 2021-12-17 2023-06-22 Basf Se Compositions and their applications
WO2023137264A1 (en) 2022-01-13 2023-07-20 Danisco Us Inc. Compositions and methods for enhanced protein production in gram‑positive bacterial cells
WO2023148086A1 (en) 2022-02-04 2023-08-10 Basf Se Compositions comprising polymers, polymers, and their use
WO2024012894A1 (en) 2022-07-15 2024-01-18 Basf Se Alkanolamine formates for enzyme stabilization in liquid formulations
WO2024083589A1 (en) 2022-10-18 2024-04-25 Basf Se Detergent compositions, polymers and methods of manufacturing the same
WO2024115754A1 (en) 2022-12-02 2024-06-06 Basf Se Aqueous compositions containing polyalkoxylates, polyalkoxylates, and use
EP4389864A1 (en) 2022-12-20 2024-06-26 Basf Se Cutinases

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
EP0108301B2 (en) * 1982-11-01 1993-09-01 SOLVAY ENZYMES, INC. (a Delaware corporation) Method of heterologous cloning of gene in bacillus microorganism
IE81141B1 (en) * 1983-06-24 2000-04-05 Genencor Int Procaryotic carbonyl hydrolases
EP0134048B2 (en) * 1983-07-06 1996-08-14 Gist-Brocades N.V. Molecular cloning and expression in industrial microorganism species
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
EP0254735B2 (en) * 1986-01-15 1998-06-17 Amgen Inc. METHOD FOR PRODUCTION OF THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS
SG30639G (en) * 1986-04-30 1995-09-01 Genencor Int Non-human carbonyl hydrolase mutants DNA sequences and vectors encoding same and hosts transformed with said vectors
HUT50877A (en) * 1987-02-27 1990-03-28 Gist Brocades Nv Process for producing stable gene amplification in chromosomal dna of procaryote microorganisms

Also Published As

Publication number Publication date
EP0283075B1 (en) 1999-05-12
EP0896062A2 (en) 1999-02-10
EP0479396A3 (en) 1992-05-13
EP0283075A3 (en) 1988-12-14
EP0479396A2 (en) 1992-04-08
EP0479396B1 (en) 1999-06-09
ES2135386T3 (es) 1999-11-01
EP0896062A3 (en) 1999-04-07
EP0283075A2 (en) 1988-09-21

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