DE291194T1 - Immunoassays und vorrichtungen dafuer. - Google Patents
Immunoassays und vorrichtungen dafuer.Info
- Publication number
- DE291194T1 DE291194T1 DE198888303744T DE88303744T DE291194T1 DE 291194 T1 DE291194 T1 DE 291194T1 DE 198888303744 T DE198888303744 T DE 198888303744T DE 88303744 T DE88303744 T DE 88303744T DE 291194 T1 DE291194 T1 DE 291194T1
- Authority
- DE
- Germany
- Prior art keywords
- carrier
- reagent
- specific binding
- housing
- region
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000003018 immunoassay Methods 0.000 title 1
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract 23
- 230000009870 specific binding Effects 0.000 claims abstract 14
- 239000000463 material Substances 0.000 claims abstract 9
- 239000007788 liquid Substances 0.000 claims abstract 8
- 239000012876 carrier material Substances 0.000 claims abstract 6
- 238000004458 analytical method Methods 0.000 claims abstract 3
- 229920003023 plastic Polymers 0.000 claims abstract 3
- 239000011343 solid material Substances 0.000 claims abstract 3
- 239000004033 plastic Substances 0.000 claims abstract 2
- 238000001514 detection method Methods 0.000 claims 11
- 239000000126 substance Substances 0.000 claims 8
- 239000002250 absorbent Substances 0.000 claims 4
- 230000002745 absorbent Effects 0.000 claims 4
- 210000002700 urine Anatomy 0.000 claims 4
- 239000000020 Nitrocellulose Substances 0.000 claims 3
- 239000004816 latex Substances 0.000 claims 3
- 229920000126 latex Polymers 0.000 claims 3
- 229920001220 nitrocellulos Polymers 0.000 claims 3
- 239000002245 particle Substances 0.000 claims 3
- 239000011148 porous material Substances 0.000 claims 3
- 238000000159 protein binding assay Methods 0.000 claims 2
- 238000003556 assay Methods 0.000 claims 1
- 230000016087 ovulation Effects 0.000 claims 1
- 238000009597 pregnancy test Methods 0.000 claims 1
- 239000007787 solid Substances 0.000 claims 1
- 239000012466 permeate Substances 0.000 abstract 2
- 239000012491 analyte Substances 0.000 abstract 1
- 230000035935 pregnancy Effects 0.000 abstract 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54386—Analytical elements
- G01N33/54387—Immunochromatographic test strips
- G01N33/54388—Immunochromatographic test strips based on lateral flow
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/548—Carbohydrates, e.g. dextran
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/549—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic with antigen or antibody entrapped within the carrier
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/558—Immunoassay; Biospecific binding assay; Materials therefor using diffusion or migration of antigen or antibody
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/689—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids related to pregnancy or the gonads
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/74—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving hormones or other non-cytokine intercellular protein regulatory factors such as growth factors, including receptors to hormones and growth factors
- G01N33/76—Human chorionic gonadotropin including luteinising hormone, follicle stimulating hormone, thyroid stimulating hormone or their receptors
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N2800/00—Detection or diagnosis of diseases
- G01N2800/36—Gynecology or obstetrics
- G01N2800/368—Pregnancy complicated by disease or abnormalities of pregnancy, e.g. preeclampsia, preterm labour
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N30/00—Investigating or analysing materials by separation into components using adsorption, absorption or similar phenomena or using ion-exchange, e.g. chromatography or field flow fractionation
- G01N30/90—Plate chromatography, e.g. thin layer or paper chromatography
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/805—Test papers
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/81—Packaged device or kit
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/962—Prevention or removal of interfering materials or reactants or other treatment to enhance results, e.g. determining or preventing nonspecific binding
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/969—Multiple layering of reactants
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/97—Test strip or test slide
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S435/00—Chemistry: molecular biology and microbiology
- Y10S435/975—Kit
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/805—Optical property
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/807—Apparatus included in process claim, e.g. physical support structures
- Y10S436/81—Tube, bottle, or dipstick
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
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- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/811—Test for named disease, body condition or organ function
- Y10S436/814—Pregnancy
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/815—Test for named compound or class of compounds
- Y10S436/817—Steroids or hormones
- Y10S436/818—Human chorionic gonadotropin
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/906—Fertility tests
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Immunology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Urology & Nephrology (AREA)
- General Physics & Mathematics (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Pathology (AREA)
- Endocrinology (AREA)
- Reproductive Health (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gynecology & Obstetrics (AREA)
- Pregnancy & Childbirth (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
- Measurement And Recording Of Electrical Phenomena And Electrical Characteristics Of The Living Body (AREA)
- Cleaning Or Drying Semiconductors (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Measuring Pulse, Heart Rate, Blood Pressure Or Blood Flow (AREA)
- Measurement Of Radiation (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Medicinal Preparation (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
Claims (19)
1. Analytisches Testgerät, dadurch gekennzeichnet, daß es ein hohles
_ Gehäuse (500) aus feuchtigkeitsundurchlässigem festen Material aufweist,
das einen trockenen porösen Träger (510) enthält, der direkt oder indirekt
mit der Außenseite des Gehäuses in Verbindung steht, so daß eine flüssige Testprobe auf den porösen Träger aufgebracht werden kann, daß
das Gerät auch ein markiertes spezifisches Bindungsreagenz für eine
Nachweissubstanz, welches markierte spezifische Bindungsreagenz frei in
15
dem porösen Träger beweglich ist, wenn dieser sich im feuchten Zustand
befindet, und ein unmarkiertes spezifisches Bindungsreagenz für dieselbe Nachweissubstanz enthält, welches unmarkierte Reagenz permanent in
einem Nachweisbereich auf dem Trägermaterial immobilisiert ist und
folglich auch im feuchten Zustand unbeweglich ist, wobei die relative
20
Positionierung des markierten Reagenzes und des Nachweisbereiches so
ist, daß eine Flüssigprobe, die auf das Gerät aufgebracht worden ist, markiertes Reagenz aufnehmen und danach in den Nachweisbereich
wandern kann und daß das Gerät Mittel enthält, die gegebenenfalls das
Ausmaß, in welchem das markierte Reagenz in dem Nachweisbereich 25
gebunden wird, beobachtbar machen.
2. Analytisches Testgerät, dadurch gekennzeichnet, daß es ein hohles
Gehäuse (500) aus feuchtigkeitsundurchlässigem festen Material aufweist,
das einen trockenen porösen Träger (510) enthält, der direkt oder indirekt
30
mit der Außenseite des Gehäuses in Verbindung steht, so daß eine flüssige Testprobe auf den porösen Träger aufgebracht werden kann,
welcher Träger in einem ersten Bereich ein markiertes spezifisches Bindungsreagenz
für eine Nachweissubstanz enthält, welches markierte
spezifische Bindungsreagenz frei in dem porösen Träger beweglich ist,
35
wenn dieser sich im feuchten Zustand befindet, und welcher Träger in
einem zweiten, räumlich von dem ersten Bereich getrennten Bereich ein
unmarkiertes spezifisches Bindungsreagenz für dieselbe Nachweissubstanz
enthält, welches unmarkierte Reagenz permanent auf dem Trägermaterial immobilisiert und folglich auch im feuchten Zustand unbeweglich ist, daß
die beiden Bereiche so angeordnet sind, daß eine Flüssigprobe, die auf den porösen Träger aufgebracht worden ist, über den ersten Bereich in
den zweiten Bereich wandern kann und daß das Gerät Mittel enthält, die gegebenenfalls das Ausmaß, in welchem das markierte Reagenz in dem
zweiten Bereich gebunden wird, beobachtbar machen.
3. Testgerät nach Anspruch 1 oder 2 , dadurch gekennzeichnet, daß die
Markierung an dem ersten spezifischen Bindungsreagenz eine Direktmarkierung ist.
4. Testgerät nach einem der Ansprüche 1 bis 3, dadurch gekennzeichnet,
daß das Gehäuse aus undurchsichtigem oder durchscheinendem Material besteht und mindestens mit einer Öffnung (508) versehen ist, durch die
das analytische Ergebnis beobachtet werden kann.
5. Testgerät nach einem der vorhergehenden Ansprüche, dadurch
gekennzeichnet, daß der poröse Träger (510) mit der Außenseite des
Gerätes über ein saugfähiges Probenaufnahmeteil (506) in Verbindung steht, das über das Gehäuse hinaussteht und das als Reservoir für die
Aufnahme der Flüssigprobe dient und diese in den porösen Träger abgibt.
6. Testgerät nach Anspruch 5, dadurch gekennzeichnet, daß es einen
abnehmbaren und wieder aufsetzbaren feuchtigkeitsundurchlässigen Deckel (503) für das hinausstehende saugfähige Probenaufnahmeteil (506)
aufweist.
"^ 7. Testgerät nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet,
daß das Gehäuse und gegebenenfalls der Deckel aus Kunststoffmaterial geformt ist.
8. Testgerät nach einem der vorhergehenden Ansprüche, dadurch gekenn-
^5 zeichnet, daß der poröse Träger einen Streifen oder eine Schicht aus
porösem Material (510) umfaßt, das mit einer Schicht (511) aus transparentem feuchtigkeitsundurchlässigem Material verstärkt ist, wobei
die transparente Schicht mit der Innenseite des Gehäuses an der (die)
Öffnung(en) angrenzt, um das Eintreten von Feuchtigkeit oder Probe zu
verhindern.
9. Testgerät nach Anspruch 7, dadurch gekennzeichnet, daß das poröse
Trägermaterial Nitrozellulose, vorzugsweise mit einer Porengröße von größer als etwa 1 Mikrometer, ist.
10. Testgerät nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet,
daß die Markierung farbige Latexpartikel mit einer maximalen Abmessung, die nicht größer als etwa 0,5 Mikrometer ist,
aufweist.
11. Testgerät nach einem der vorhergehenden Ansprüche, dadurch gekennzeichnet,
daß es einen Kontrollbereich stromabwärts von dem zweiten Bereich in dem porösen Träger aufweist, um anzuzeigen, daß die
Flüssigprobe über den zweiten Bereich hinaus gewandert ist, welcher Kontrollbereich ebenfalls von der Außenseite des Gehäuses beobachtbar
ist.
20
20
12. Schwangerschaftstestgerät, dadurch gekennzeichnet, daß es ein hohles, längliches Gehäuse aufweist, das einen trockenen porösen Nitrozelluloseträger
enthält, der indirekt mit der Außenseite des Gehäuses über ein saugfähiges Urinaufnahmeteil in Verbindung steht, das über das
Gehäuse hinaussteht und als Reservoir, von dem Urin in den porösen
Träger abgegeben wird, dienen kann, daß der Träger in einem ersten Bereich einen hochspezifischen Anti-hCG-Antikörper enthält, der eine
farbige Direktmarkierung trägt, welcher markierte Antikörper frei beweglich in dem porösen Träger ist, wenn sich dieser im feuchten
Zustand befindet, daß der Träger in einem zweiten, räumlich von dem ersten Bereich getrennten Bereich einen hochspezifischen unmarkierten
Anti-hCG-Antikörper enthält, der permanent auf dem Trägermaterial immobilisiert ist und daher im feuchten Zustand nicht beweglich ist, daß
die markierten und unmarkierten Antikörper Spezifitäten für
" unterschiedliche hCG-Epitope aufweisen, daß die beiden Bereiche so
angeordnet sind, daß eine Urinprobe, die auf den porösen Träger aufgebracht
worden ist, über den ersten Bereich in den zweiten Bereich
wandern kann, daß das Gehäuse aus undurchsichtigem oder durchscheinendem Material besteht und mindestens eine Öffnung aufweist,
Ji durch die das analytische Ergebnis beobachtet werden kann, und daß das
Gehäuse einen entfernbaren und wieder aufsetzbaren Deckel für das hinausstehende saugfähige Urinaufnahmeteil aufweist.
13. Ovulationstestgerät, das alle Merkmale des Gerätes nach Anspruch
12 aufweist, wobei jedoch die Nachweissubstanz LH ist.
14. Trockener poröser Träger zur Verwendung in einem spezifischen Bindungsassay, der einen Streifen oder eine Schicht aus Nitrozellulose
mit einer Porengröße von mindestens etwa 1 Mikrometer, vorzugsweise von etwa 8 bis 12 Mikrometern, aufweist und der in einem Bereich ein
immobilisiertes spezifisches Bindungsreagenz für eine Nachweissubstanz
trägt.
15. Träger nach Anspruch 14, der durch eine Schicht aus festem wasserundurchlässigem
Material, vorzugsweise transparentem Kunststoffmaterial, verstärkt ist.
16* Träger nach Anspruch 15, der in einem anderen Bereich ein
markiertes spezifisches Bindungsreagenz für die gleiche Nachweissubstanz trägt, wobei das markierte spezifische Bindungsreagenz innerhalb des
Trägermaterials frei beweglich ist, wenn dieses sich im feuchten Zustand
befindet.
17. Träger nach Anspruch 16, worin die Markierung eine Direktmarkierung,
vorzugsweise ein farbiges Latexpartikel mit einer maximalen Abmessung von nicht größer als etwa 0,5 Mikrometern, ist.
18. Träger nach Anspruch 17, worin der Bereich, der das markierte
Reagenz enthält, vor dem Auftragen des markierten Reagenzes mit einem Glasurmaterial, wie z.B. Zucker, vorbehandelt worden ist.
19. Spezifisches Bindungsassay, das die Verwendung eines markierten
Reagenzes umfaßt, das durch ein poröses Trägermaterial wandern kann, das durch Aufbringen einer wäßrigen Probe, die möglicherweise eine
Nachweissubstanz enthält, befeuchtet worden ist, dadurch gekennzeichnet,
daß die Markierung eine Direktmarkierung, vorzugsweise ein farbiges Latexpartikel, ist.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
GB878709873A GB8709873D0 (en) | 1987-04-27 | 1987-04-27 | Assays |
GB878725457A GB8725457D0 (en) | 1987-10-30 | 1987-10-30 | Assays |
Publications (1)
Publication Number | Publication Date |
---|---|
DE291194T1 true DE291194T1 (de) | 1992-03-19 |
Family
ID=26292175
Family Applications (7)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE1248112T Pending DE1248112T1 (de) | 1987-04-27 | 1988-04-26 | Immunochromatographische Spezifischebindungsassayvorrichtung |
DE3856542T Expired - Lifetime DE3856542T2 (de) | 1987-04-27 | 1988-04-26 | Testgerät zur Durchführung von spezifischen Bindungsprüfungen |
DE3856421T Expired - Lifetime DE3856421T2 (de) | 1987-04-27 | 1988-04-26 | Spezifische Bindungstestverfahren |
DE0560410T Pending DE560410T1 (de) | 1987-04-27 | 1988-04-26 | Testgerät zur Durchführung von spezifischen Bindungsprüfungen. |
DE198888303744T Pending DE291194T1 (de) | 1987-04-27 | 1988-04-26 | Immunoassays und vorrichtungen dafuer. |
DE3887771T Expired - Lifetime DE3887771C5 (de) | 1987-04-27 | 1988-04-26 | Immunoassays und Vorrichtungen dafür. |
DE8805565U Expired DE8805565U1 (de) | 1987-04-27 | 1988-04-27 | Analytisches Testgerät |
Family Applications Before (4)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE1248112T Pending DE1248112T1 (de) | 1987-04-27 | 1988-04-26 | Immunochromatographische Spezifischebindungsassayvorrichtung |
DE3856542T Expired - Lifetime DE3856542T2 (de) | 1987-04-27 | 1988-04-26 | Testgerät zur Durchführung von spezifischen Bindungsprüfungen |
DE3856421T Expired - Lifetime DE3856421T2 (de) | 1987-04-27 | 1988-04-26 | Spezifische Bindungstestverfahren |
DE0560410T Pending DE560410T1 (de) | 1987-04-27 | 1988-04-26 | Testgerät zur Durchführung von spezifischen Bindungsprüfungen. |
Family Applications After (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE3887771T Expired - Lifetime DE3887771C5 (de) | 1987-04-27 | 1988-04-26 | Immunoassays und Vorrichtungen dafür. |
DE8805565U Expired DE8805565U1 (de) | 1987-04-27 | 1988-04-27 | Analytisches Testgerät |
Country Status (12)
Country | Link |
---|---|
US (6) | US5602040A (de) |
EP (5) | EP1248112A3 (de) |
JP (4) | JPH0746107B2 (de) |
AT (2) | ATE195022T1 (de) |
AU (4) | AU626207B2 (de) |
DE (7) | DE1248112T1 (de) |
ES (3) | ES2150428T3 (de) |
FR (1) | FR2614423B3 (de) |
GB (1) | GB2204398B (de) |
HK (1) | HK140995A (de) |
IT (1) | IT214285Z2 (de) |
WO (1) | WO1988008534A1 (de) |
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-
1988
- 1988-04-26 EP EP02008106A patent/EP1248112A3/de not_active Withdrawn
- 1988-04-26 DE DE1248112T patent/DE1248112T1/de active Pending
- 1988-04-26 DE DE3856542T patent/DE3856542T2/de not_active Expired - Lifetime
- 1988-04-26 ES ES93108764T patent/ES2150428T3/es not_active Expired - Lifetime
- 1988-04-26 AT AT93108764T patent/ATE195022T1/de not_active IP Right Cessation
- 1988-04-26 WO PCT/GB1988/000322 patent/WO1988008534A1/en unknown
- 1988-04-26 DE DE3856421T patent/DE3856421T2/de not_active Expired - Lifetime
- 1988-04-26 DE DE0560410T patent/DE560410T1/de active Pending
- 1988-04-26 ES ES88303744T patent/ES2050704T5/es not_active Expired - Lifetime
- 1988-04-26 DE DE198888303744T patent/DE291194T1/de active Pending
- 1988-04-26 GB GB8809867A patent/GB2204398B/en not_active Expired - Lifetime
- 1988-04-26 EP EP93108763A patent/EP0560410B1/de not_active Expired - Lifetime
- 1988-04-26 EP EP93108764A patent/EP0560411B1/de not_active Expired - Lifetime
- 1988-04-26 AU AU16228/88A patent/AU626207B2/en not_active Expired
- 1988-04-26 AT AT93108763T patent/ATE225509T1/de not_active IP Right Cessation
- 1988-04-26 ES ES93108763T patent/ES2184734T3/es not_active Expired - Lifetime
- 1988-04-26 EP EP88303744A patent/EP0291194B8/de not_active Expired - Lifetime
- 1988-04-26 DE DE3887771T patent/DE3887771C5/de not_active Expired - Lifetime
- 1988-04-26 JP JP63503518A patent/JPH0746107B2/ja not_active Expired - Lifetime
- 1988-04-26 EP EP05007050A patent/EP1555529A3/de not_active Withdrawn
- 1988-04-27 DE DE8805565U patent/DE8805565U1/de not_active Expired
- 1988-04-27 IT IT8853100U patent/IT214285Z2/it active
- 1988-04-27 FR FR888805600A patent/FR2614423B3/fr not_active Expired
-
1993
- 1993-06-22 JP JP5150881A patent/JP2705767B2/ja not_active Expired - Lifetime
- 1993-06-22 JP JP5150882A patent/JP2705768B2/ja not_active Expired - Lifetime
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1994
- 1994-05-12 US US08/241,675 patent/US5602040A/en not_active Expired - Lifetime
- 1994-12-16 AU AU80490/94A patent/AU679279B2/en not_active Withdrawn - After Issue
- 1994-12-16 AU AU80489/94A patent/AU682071B2/en not_active Expired
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1995
- 1995-06-07 US US08/474,192 patent/US6228660B1/en not_active Expired - Lifetime
- 1995-06-07 US US08/487,319 patent/US6187598B1/en not_active Expired - Lifetime
- 1995-09-07 HK HK140995A patent/HK140995A/xx not_active IP Right Cessation
-
1996
- 1996-10-25 JP JP8284355A patent/JP2919392B2/ja not_active Expired - Lifetime
-
1997
- 1997-11-07 AU AU44386/97A patent/AU4438697A/en not_active Abandoned
-
2001
- 2001-02-12 US US09/780,351 patent/US7109042B2/en not_active Expired - Fee Related
- 2001-02-28 US US09/794,061 patent/US6818455B2/en not_active Expired - Fee Related
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2004
- 2004-11-16 US US10/990,946 patent/US20050244986A1/en not_active Abandoned
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