CN104293697A - 一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 - Google Patents
一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 Download PDFInfo
- Publication number
- CN104293697A CN104293697A CN201410476593.9A CN201410476593A CN104293697A CN 104293697 A CN104293697 A CN 104293697A CN 201410476593 A CN201410476593 A CN 201410476593A CN 104293697 A CN104293697 A CN 104293697A
- Authority
- CN
- China
- Prior art keywords
- enterococcus faecalis
- active bacteria
- chicken feed
- probiotic agent
- seed
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 239000006041 probiotic Substances 0.000 title claims abstract description 74
- 235000018291 probiotics Nutrition 0.000 title claims abstract description 74
- 241000287828 Gallus gallus Species 0.000 title claims abstract description 72
- 230000000529 probiotic effect Effects 0.000 title claims abstract description 69
- 241000194032 Enterococcus faecalis Species 0.000 title claims abstract description 66
- 229940032049 enterococcus faecalis Drugs 0.000 title claims abstract description 65
- 239000003795 chemical substances by application Substances 0.000 title claims abstract description 60
- 238000002360 preparation method Methods 0.000 title claims abstract description 37
- 238000002156 mixing Methods 0.000 claims abstract description 32
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims abstract description 30
- 230000006641 stabilisation Effects 0.000 claims abstract description 17
- 238000011105 stabilization Methods 0.000 claims abstract description 17
- 239000011248 coating agent Substances 0.000 claims abstract description 11
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 6
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 6
- 238000001035 drying Methods 0.000 claims abstract description 6
- 241000894006 Bacteria Species 0.000 claims description 95
- 239000002609 medium Substances 0.000 claims description 38
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- 239000007788 liquid Substances 0.000 claims description 33
- 238000000855 fermentation Methods 0.000 claims description 25
- 230000004151 fermentation Effects 0.000 claims description 25
- 239000000463 material Substances 0.000 claims description 19
- 239000000203 mixture Substances 0.000 claims description 14
- 239000002054 inoculum Substances 0.000 claims description 13
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 claims description 12
- 230000001681 protective effect Effects 0.000 claims description 12
- 229940041514 candida albicans extract Drugs 0.000 claims description 11
- 239000012138 yeast extract Substances 0.000 claims description 11
- 239000003094 microcapsule Substances 0.000 claims description 10
- 238000001238 wet grinding Methods 0.000 claims description 10
- 239000001963 growth medium Substances 0.000 claims description 9
- 239000002994 raw material Substances 0.000 claims description 9
- 238000003756 stirring Methods 0.000 claims description 9
- 239000000654 additive Substances 0.000 claims description 7
- YXVFQADLFFNVDS-UHFFFAOYSA-N diammonium citrate Chemical compound [NH4+].[NH4+].[O-]C(=O)CC(O)(C(=O)O)CC([O-])=O YXVFQADLFFNVDS-UHFFFAOYSA-N 0.000 claims description 7
- AIUDWMLXCFRVDR-UHFFFAOYSA-N dimethyl 2-(3-ethyl-3-methylpentyl)propanedioate Chemical compound CCC(C)(CC)CCC(C(=O)OC)C(=O)OC AIUDWMLXCFRVDR-UHFFFAOYSA-N 0.000 claims description 7
- 239000003651 drinking water Substances 0.000 claims description 7
- 235000020188 drinking water Nutrition 0.000 claims description 7
- 238000011218 seed culture Methods 0.000 claims description 7
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 6
- 229920002307 Dextran Polymers 0.000 claims description 6
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- 229920000168 Microcrystalline cellulose Polymers 0.000 claims description 6
- 235000011187 glycerol Nutrition 0.000 claims description 6
- 238000011081 inoculation Methods 0.000 claims description 6
- 238000009413 insulation Methods 0.000 claims description 6
- 239000008101 lactose Substances 0.000 claims description 6
- 229940016286 microcrystalline cellulose Drugs 0.000 claims description 6
- 235000019813 microcrystalline cellulose Nutrition 0.000 claims description 6
- 239000008108 microcrystalline cellulose Substances 0.000 claims description 6
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 claims description 5
- AYRXSINWFIIFAE-SCLMCMATSA-N Isomaltose Natural products OC[C@H]1O[C@H](OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C=O)[C@@H](O)[C@@H](O)[C@@H]1O AYRXSINWFIIFAE-SCLMCMATSA-N 0.000 claims description 5
- 239000005913 Maltodextrin Substances 0.000 claims description 5
- 229920002774 Maltodextrin Polymers 0.000 claims description 5
- 230000000996 additive effect Effects 0.000 claims description 5
- 235000010418 carrageenan Nutrition 0.000 claims description 5
- 229920001525 carrageenan Polymers 0.000 claims description 5
- 150000001875 compounds Chemical class 0.000 claims description 5
- DLRVVLDZNNYCBX-RTPHMHGBSA-N isomaltose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1OC[C@@H]1[C@@H](O)[C@H](O)[C@@H](O)C(O)O1 DLRVVLDZNNYCBX-RTPHMHGBSA-N 0.000 claims description 5
- 229940035034 maltodextrin Drugs 0.000 claims description 5
- 239000011236 particulate material Substances 0.000 claims description 5
- 239000001814 pectin Substances 0.000 claims description 5
- 235000010987 pectin Nutrition 0.000 claims description 5
- 229920001277 pectin Polymers 0.000 claims description 5
- 235000020183 skimmed milk Nutrition 0.000 claims description 5
- 235000010413 sodium alginate Nutrition 0.000 claims description 5
- 239000000661 sodium alginate Substances 0.000 claims description 5
- 229940005550 sodium alginate Drugs 0.000 claims description 5
- 239000007921 spray Substances 0.000 claims description 5
- 238000005507 spraying Methods 0.000 claims description 5
- DPDMMXDBJGCCQC-UHFFFAOYSA-N [Na].[Cl] Chemical compound [Na].[Cl] DPDMMXDBJGCCQC-UHFFFAOYSA-N 0.000 claims description 3
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 3
- 239000013530 defoamer Substances 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 claims description 3
- 244000144977 poultry Species 0.000 claims description 3
- 238000002791 soaking Methods 0.000 claims description 3
- 238000009374 poultry farming Methods 0.000 claims description 2
- 230000004913 activation Effects 0.000 claims 2
- 241000235070 Saccharomyces Species 0.000 claims 1
- 241001465754 Metazoa Species 0.000 abstract description 31
- 238000004519 manufacturing process Methods 0.000 abstract description 24
- 230000001580 bacterial effect Effects 0.000 abstract description 13
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 abstract description 7
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 230000001850 reproductive effect Effects 0.000 abstract description 5
- 238000010521 absorption reaction Methods 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000008901 benefit Effects 0.000 abstract description 2
- 230000035764 nutrition Effects 0.000 abstract description 2
- 230000002829 reductive effect Effects 0.000 abstract description 2
- 239000010802 sludge Substances 0.000 abstract 3
- 238000000576 coating method Methods 0.000 abstract 1
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract 1
- 238000005453 pelletization Methods 0.000 abstract 1
- 239000003223 protective agent Substances 0.000 abstract 1
- 235000013330 chicken meat Nutrition 0.000 description 50
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 description 40
- 238000012360 testing method Methods 0.000 description 27
- 239000000243 solution Substances 0.000 description 25
- 235000014655 lactic acid Nutrition 0.000 description 20
- 235000013601 eggs Nutrition 0.000 description 17
- 241000194031 Enterococcus faecium Species 0.000 description 14
- 239000000843 powder Substances 0.000 description 14
- 238000000034 method Methods 0.000 description 12
- 239000004310 lactic acid Substances 0.000 description 10
- 235000014590 basal diet Nutrition 0.000 description 9
- 235000013372 meat Nutrition 0.000 description 9
- 230000008569 process Effects 0.000 description 9
- 238000009395 breeding Methods 0.000 description 8
- 230000001488 breeding effect Effects 0.000 description 8
- 241000272525 Anas platyrhynchos Species 0.000 description 7
- 241000272517 Anseriformes Species 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 7
- 102000004169 proteins and genes Human genes 0.000 description 7
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 6
- 239000008103 glucose Substances 0.000 description 6
- 230000036039 immunity Effects 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 230000012447 hatching Effects 0.000 description 5
- 244000144972 livestock Species 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 4
- 108010062877 Bacteriocins Proteins 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000003242 anti bacterial agent Substances 0.000 description 4
- 229940088710 antibiotic agent Drugs 0.000 description 4
- 230000000968 intestinal effect Effects 0.000 description 4
- 244000005700 microbiome Species 0.000 description 4
- 230000004584 weight gain Effects 0.000 description 4
- 235000019786 weight gain Nutrition 0.000 description 4
- 208000035240 Disease Resistance Diseases 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 244000052616 bacterial pathogen Species 0.000 description 3
- 230000035558 fertility Effects 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 108090000765 processed proteins & peptides Proteins 0.000 description 3
- 241000607528 Aeromonas hydrophila Species 0.000 description 2
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000193830 Bacillus <bacterium> Species 0.000 description 2
- 241000726221 Gemma Species 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 2
- 241000606856 Pasteurella multocida Species 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 244000046052 Phaseolus vulgaris Species 0.000 description 2
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 2
- 241000589517 Pseudomonas aeruginosa Species 0.000 description 2
- 241000607142 Salmonella Species 0.000 description 2
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 2
- 241000191967 Staphylococcus aureus Species 0.000 description 2
- 229930006000 Sucrose Natural products 0.000 description 2
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 description 2
- 240000008042 Zea mays Species 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000003042 antagnostic effect Effects 0.000 description 2
- 230000000845 anti-microbial effect Effects 0.000 description 2
- 230000002180 anti-stress Effects 0.000 description 2
- 230000003115 biocidal effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 230000007850 degeneration Effects 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 210000002969 egg yolk Anatomy 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000003203 everyday effect Effects 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 230000000813 microbial effect Effects 0.000 description 2
- 244000000010 microbial pathogen Species 0.000 description 2
- 235000013379 molasses Nutrition 0.000 description 2
- 231100000252 nontoxic Toxicity 0.000 description 2
- 230000003000 nontoxic effect Effects 0.000 description 2
- 229940051027 pasteurella multocida Drugs 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- 230000001766 physiological effect Effects 0.000 description 2
- 235000010482 polyoxyethylene sorbitan monooleate Nutrition 0.000 description 2
- 229920000053 polysorbate 80 Polymers 0.000 description 2
- 235000013594 poultry meat Nutrition 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000000452 restraining effect Effects 0.000 description 2
- 230000028327 secretion Effects 0.000 description 2
- 210000000582 semen Anatomy 0.000 description 2
- 238000004088 simulation Methods 0.000 description 2
- 239000001632 sodium acetate Substances 0.000 description 2
- 229960004249 sodium acetate Drugs 0.000 description 2
- 235000017281 sodium acetate Nutrition 0.000 description 2
- 239000005720 sucrose Substances 0.000 description 2
- 238000003786 synthesis reaction Methods 0.000 description 2
- 238000009423 ventilation Methods 0.000 description 2
- NWXMGUDVXFXRIG-WESIUVDSSA-N (4s,4as,5as,6s,12ar)-4-(dimethylamino)-1,6,10,11,12a-pentahydroxy-6-methyl-3,12-dioxo-4,4a,5,5a-tetrahydrotetracene-2-carboxamide Chemical compound C1=CC=C2[C@](O)(C)[C@H]3C[C@H]4[C@H](N(C)C)C(=O)C(C(N)=O)=C(O)[C@@]4(O)C(=O)C3=C(O)C2=C1O NWXMGUDVXFXRIG-WESIUVDSSA-N 0.000 description 1
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 241000186000 Bifidobacterium Species 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 102000016938 Catalase Human genes 0.000 description 1
- 108010053835 Catalase Proteins 0.000 description 1
- 241001478240 Coccus Species 0.000 description 1
- 208000004232 Enteritis Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 201000008225 Klebsiella pneumonia Diseases 0.000 description 1
- 241000588747 Klebsiella pneumoniae Species 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000237502 Ostreidae Species 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 206010035717 Pneumonia klebsiella Diseases 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000607758 Shigella sp. Species 0.000 description 1
- 241001052560 Thallis Species 0.000 description 1
- 241001331543 Veillonella sp. Species 0.000 description 1
- 229930003270 Vitamin B Natural products 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000001070 adhesive effect Effects 0.000 description 1
- 150000001408 amides Chemical class 0.000 description 1
- 235000001014 amino acid Nutrition 0.000 description 1
- 235000019789 appetite Nutrition 0.000 description 1
- 230000036528 appetite Effects 0.000 description 1
- 238000009360 aquaculture Methods 0.000 description 1
- 244000144974 aquaculture Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- FFBHFFJDDLITSX-UHFFFAOYSA-N benzyl N-[2-hydroxy-4-(3-oxomorpholin-4-yl)phenyl]carbamate Chemical compound OC1=C(NC(=O)OCC2=CC=CC=C2)C=CC(=C1)N1CCOCC1=O FFBHFFJDDLITSX-UHFFFAOYSA-N 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000037396 body weight Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229960005069 calcium Drugs 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- MKJXYGKVIBWPFZ-CEOVSRFSSA-L calcium;(2s)-2-hydroxypropanoate Chemical compound [Ca+2].C[C@H](O)C([O-])=O.C[C@H](O)C([O-])=O MKJXYGKVIBWPFZ-CEOVSRFSSA-L 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 238000006555 catalytic reaction Methods 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 230000000739 chaotic effect Effects 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 230000002354 daily effect Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 235000019621 digestibility Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 201000006549 dyspepsia Diseases 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000012631 food intake Nutrition 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 210000002540 macrophage Anatomy 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 235000021590 normal diet Nutrition 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 235000020636 oyster Nutrition 0.000 description 1
- 230000036961 partial effect Effects 0.000 description 1
- 238000011056 performance test Methods 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 230000035479 physiological effects, processes and functions Effects 0.000 description 1
- 235000002949 phytic acid Nutrition 0.000 description 1
- 230000002335 preservative effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 239000004460 silage Substances 0.000 description 1
- 235000000053 special nutrition Nutrition 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005728 strengthening Methods 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 235000021195 test diet Nutrition 0.000 description 1
- 238000012546 transfer Methods 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000019156 vitamin B Nutrition 0.000 description 1
- 239000011720 vitamin B Substances 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
- 235000015099 wheat brans Nutrition 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Mycology (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Botany (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Fodder In General (AREA)
Abstract
Description
组别 | 对照组 | 试验组 |
产蛋率(%) | 77.14±2.08a | 78.53±2.03a |
蛋重(g/枚) | 60.25±1.46a | 60.93±2.31a |
采食量(g/只/天) | 163.52±6.78a | 161.24±7.89a |
料蛋比 | 3.91±0.19b | 2.94±0.03a |
死淘率(%) | 0.07±0.00b | 0.03±0.00a |
种蛋合格率(%) | 92.66±1.46a | 94.53±2.07b |
组别 | 对照组 | 试验组 |
种蛋受精率(%) | 90.12±1.27a | 92.37±1.74b |
种蛋孵化率(%) | 93.41±1.46a | 94.03±2.01a |
健雏率(%) | 92.49±2.89a | 94.79±2.47b |
组别 | 对照组 | 试验组 |
总耗料(Kg) | 8393.56±58.02 | 8481.33±79.08 |
出栏总重(Kg) | 5019.76±11.45 | 5205.24±20.64 |
出栏只均重(Kg) | 2.67±0.24a | 2.94±0.31b |
只均耗料(Kg) | 4.34±0.03 | 4.29±0.04 |
料重比 | 1.88±0.03a | 1.71±0.08b |
进栏数(只) | 2000 | 2000 |
出栏数(只) | 1934 | 1977 |
成活率(%) | 96.7% | 98.85% |
Claims (10)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410476593.9A CN104293697B (zh) | 2014-09-16 | 2014-09-16 | 一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201410476593.9A CN104293697B (zh) | 2014-09-16 | 2014-09-16 | 一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN104293697A true CN104293697A (zh) | 2015-01-21 |
CN104293697B CN104293697B (zh) | 2016-11-16 |
Family
ID=52313655
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201410476593.9A Active CN104293697B (zh) | 2014-09-16 | 2014-09-16 | 一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN104293697B (zh) |
Cited By (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN104996740A (zh) * | 2015-08-25 | 2015-10-28 | 中农颖泰林州生物科园有限公司 | 一种闪蒸干燥制备饲料用复合微生态粉的方法 |
CN105002155A (zh) * | 2015-05-26 | 2015-10-28 | 北京好实沃生物技术有限公司 | 一种猪用益生菌剂及其制备方法 |
CN105029099A (zh) * | 2015-08-28 | 2015-11-11 | 河南牧业经济学院 | 肉鸡生长后期用营养型降死亡率制剂 |
CN105985916A (zh) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | 一种复合微生物制剂及其制备方法 |
CN106047769A (zh) * | 2016-08-04 | 2016-10-26 | 北京好实沃生物技术有限公司 | 一种含凝结芽孢杆菌的复合菌剂及其制备方法 |
CN106306364A (zh) * | 2016-07-20 | 2017-01-11 | 洛阳欧科拜克生物技术股份有限公司 | 一种用于饲料添加剂的粪肠球菌产品及其生产工艺 |
CN106509471A (zh) * | 2016-11-29 | 2017-03-22 | 岳从军 | 一种促进肉鸡育肥期肠道发育用饲料 |
CN106798159A (zh) * | 2017-02-07 | 2017-06-06 | 山东省科学院生物研究所 | 饲料添加剂及在制备提高肉鸡双糖酶活性饲料中的应用 |
CN107279469A (zh) * | 2017-07-03 | 2017-10-24 | 青岛玛斯特生物技术有限公司 | 一种复合微生物制剂及其在水产饲料后喷涂中应用 |
CN108391758A (zh) * | 2018-04-25 | 2018-08-14 | 广西康佳龙农牧集团有限公司 | 哺乳母猪复方合生素 |
CN109292984A (zh) * | 2018-10-09 | 2019-02-01 | 浙江海洋大学 | 水产养殖用水质改良剂及其制备方法 |
CN109418506A (zh) * | 2017-08-23 | 2019-03-05 | 北京农博微生物科技有限公司 | 一种肠溶性饲用生物维生素的稳定包被技术 |
CN110628683A (zh) * | 2019-10-22 | 2019-12-31 | 江苏恒丰强生物技术有限公司 | 一种用于防治鸡腹泻的益生菌复合活菌制剂及其制备方法 |
CN111018133A (zh) * | 2019-12-20 | 2020-04-17 | 深圳清谷环境科技有限公司 | 一种环保微生物污水处理剂及其制备方法和应用 |
CN113462620A (zh) * | 2021-08-26 | 2021-10-01 | 福建傲农生物科技集团股份有限公司 | 一种饲用复合菌剂的制备方法及其应用 |
CN114231445A (zh) * | 2021-11-30 | 2022-03-25 | 唐山仟客莱生物科技有限公司 | 一种复合益生菌的混合发酵培养基及其应用 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1771831A (zh) * | 2004-11-12 | 2006-05-17 | 浙江大舜恒益生物技术有限公司 | 一种饲料级微生物添加剂及其制备与应用 |
CN102277325A (zh) * | 2011-08-12 | 2011-12-14 | 北京金泰得生物科技股份有限公司 | 一株饲用粪肠球菌及其应用 |
CN102409015A (zh) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | 一种复合微生态制剂及其在饲料添加剂中的应用和预混料 |
CN102742728A (zh) * | 2012-06-15 | 2012-10-24 | 北京大北农科技集团股份有限公司 | 畜禽用复合益生菌饲料添加剂及预混料、浓缩料和配合料 |
-
2014
- 2014-09-16 CN CN201410476593.9A patent/CN104293697B/zh active Active
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN1771831A (zh) * | 2004-11-12 | 2006-05-17 | 浙江大舜恒益生物技术有限公司 | 一种饲料级微生物添加剂及其制备与应用 |
CN102277325A (zh) * | 2011-08-12 | 2011-12-14 | 北京金泰得生物科技股份有限公司 | 一株饲用粪肠球菌及其应用 |
CN102409015A (zh) * | 2011-12-06 | 2012-04-11 | 北京大北农科技集团股份有限公司 | 一种复合微生态制剂及其在饲料添加剂中的应用和预混料 |
CN102742728A (zh) * | 2012-06-15 | 2012-10-24 | 北京大北农科技集团股份有限公司 | 畜禽用复合益生菌饲料添加剂及预混料、浓缩料和配合料 |
Non-Patent Citations (2)
Title |
---|
LAKSHMIKANTHA H. CHANNAIAH ET AL: "Survival of Enterococcus faecalis OG1RF:pCF10 in poultry and cattle feed: vector competence of the red flour beetle, Tribolium castaneum(Herbst)", 《JOURNAL OF FOOD PROTECTION》 * |
杜志琳等: "1株产细菌素粪肠球菌的分离鉴定", 《饲料研究》 * |
Cited By (21)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105985916A (zh) * | 2015-01-28 | 2016-10-05 | 河南惠通天下动物药业有限公司 | 一种复合微生物制剂及其制备方法 |
CN105002155A (zh) * | 2015-05-26 | 2015-10-28 | 北京好实沃生物技术有限公司 | 一种猪用益生菌剂及其制备方法 |
CN105002155B (zh) * | 2015-05-26 | 2018-06-29 | 北京好实沃生物技术有限公司 | 一种猪用益生菌剂及其制备方法 |
CN104996740B (zh) * | 2015-08-25 | 2018-05-25 | 林州中农颖泰生物肽有限公司 | 一种闪蒸干燥制备饲料用复合微生态粉的方法 |
CN104996740A (zh) * | 2015-08-25 | 2015-10-28 | 中农颖泰林州生物科园有限公司 | 一种闪蒸干燥制备饲料用复合微生态粉的方法 |
CN105029099A (zh) * | 2015-08-28 | 2015-11-11 | 河南牧业经济学院 | 肉鸡生长后期用营养型降死亡率制剂 |
CN106306364A (zh) * | 2016-07-20 | 2017-01-11 | 洛阳欧科拜克生物技术股份有限公司 | 一种用于饲料添加剂的粪肠球菌产品及其生产工艺 |
CN106047769A (zh) * | 2016-08-04 | 2016-10-26 | 北京好实沃生物技术有限公司 | 一种含凝结芽孢杆菌的复合菌剂及其制备方法 |
CN106047769B (zh) * | 2016-08-04 | 2020-01-31 | 北京好实沃生物技术有限公司 | 一种含凝结芽孢杆菌的复合菌剂及其制备方法 |
CN106509471A (zh) * | 2016-11-29 | 2017-03-22 | 岳从军 | 一种促进肉鸡育肥期肠道发育用饲料 |
CN106798159A (zh) * | 2017-02-07 | 2017-06-06 | 山东省科学院生物研究所 | 饲料添加剂及在制备提高肉鸡双糖酶活性饲料中的应用 |
CN107279469A (zh) * | 2017-07-03 | 2017-10-24 | 青岛玛斯特生物技术有限公司 | 一种复合微生物制剂及其在水产饲料后喷涂中应用 |
CN109418506A (zh) * | 2017-08-23 | 2019-03-05 | 北京农博微生物科技有限公司 | 一种肠溶性饲用生物维生素的稳定包被技术 |
CN108391758A (zh) * | 2018-04-25 | 2018-08-14 | 广西康佳龙农牧集团有限公司 | 哺乳母猪复方合生素 |
CN109292984A (zh) * | 2018-10-09 | 2019-02-01 | 浙江海洋大学 | 水产养殖用水质改良剂及其制备方法 |
CN110628683A (zh) * | 2019-10-22 | 2019-12-31 | 江苏恒丰强生物技术有限公司 | 一种用于防治鸡腹泻的益生菌复合活菌制剂及其制备方法 |
CN111018133A (zh) * | 2019-12-20 | 2020-04-17 | 深圳清谷环境科技有限公司 | 一种环保微生物污水处理剂及其制备方法和应用 |
CN111018133B (zh) * | 2019-12-20 | 2022-03-29 | 深圳清谷环境科技有限公司 | 一种环保微生物污水处理剂及其制备方法和应用 |
CN113462620A (zh) * | 2021-08-26 | 2021-10-01 | 福建傲农生物科技集团股份有限公司 | 一种饲用复合菌剂的制备方法及其应用 |
CN114231445A (zh) * | 2021-11-30 | 2022-03-25 | 唐山仟客莱生物科技有限公司 | 一种复合益生菌的混合发酵培养基及其应用 |
CN114231445B (zh) * | 2021-11-30 | 2024-03-26 | 唐山仟客莱生物科技有限公司 | 一种复合益生菌的混合发酵培养基及其应用 |
Also Published As
Publication number | Publication date |
---|---|
CN104293697B (zh) | 2016-11-16 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN104293697B (zh) | 一种含粪肠球菌的鸡饲料益生菌剂及其制备方法 | |
CN102168045B (zh) | 一种枯草芽孢杆菌制剂及其制备方法 | |
CN103598447B (zh) | 一种无抗乳猪教槽料及其制备方法 | |
CN104293696B (zh) | 一株粪肠球菌hew-a131及其应用 | |
CN102409015B (zh) | 一种复合微生态制剂及其在饲料添加剂中的应用和预混料 | |
CN101485402B (zh) | 一种早期断奶肉羊育肥复合生物饲料添加剂 | |
CN102226162B (zh) | 复合微生物饲料添加剂的制备方法及其应用 | |
CN103783315B (zh) | 一种微生态添加剂及其饲料 | |
CN102907563B (zh) | 一种畜牧养殖用高活性益生菌制剂的制备方法 | |
CN106962609A (zh) | 一种直投式生物发酵饲料添加剂及其制备方法和用途 | |
CN103907749A (zh) | 生物发酵活性载体型复合预混合饲料及其制备方法和应用 | |
CN101019609A (zh) | 利用豆粕生产纳豆芽孢杆菌饲料添加剂的固态发酵方法 | |
CN103039754A (zh) | 一种提高蛋鸡生产性能和鸡蛋品质的配合饲料 | |
CN102965316B (zh) | 含类细菌素与乳酸片球菌的饲料添加剂,预混料及配合料 | |
CN105002155A (zh) | 一种猪用益生菌剂及其制备方法 | |
CN101209088A (zh) | 一种促进仔猪生长和发育的微生物多酶添加剂 | |
CN102696860A (zh) | 基于醋糟和杂粕的一种高效低成本的微生物饲料蛋白 | |
CN105010770A (zh) | 一种羔羊教槽料及其羔羊教槽方法 | |
CN106721261A (zh) | 一种用于猪饲养用混合发酵纤维饲料及其制备方法 | |
CN106173361A (zh) | 以牧草为原料二次发酵生产牛羊全价饲料的方法 | |
CN106721026A (zh) | 一种无抗蛋鸡产蛋期复合预混合饲料及其制备方法与应用 | |
CN102517227B (zh) | 粪肠球菌及其应用与其饲料添加剂和发酵剂 | |
CN108835444A (zh) | 种鹅饲料、其制备方法、应用以及禽用食品 | |
CN102919557A (zh) | 一种生长育肥猪用益生素复合预混料及配合料 | |
CN102907585A (zh) | 一种中猪用益生素维生素预混料及配合料 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
C06 | Publication | ||
PB01 | Publication | ||
C10 | Entry into substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
C14 | Grant of patent or utility model | ||
GR01 | Patent grant | ||
C41 | Transfer of patent application or patent right or utility model | ||
TR01 | Transfer of patent right |
Effective date of registration: 20161228 Address after: 100044 Beijing city Haidian District sorghum Bridge Street No. 59, No. 1 Building 9 layer 903 Patentee after: Beijing Haoshiwo Biological Technology Co., Ltd. Address before: 100044 Beijing city Haidian District sorghum Bridge Street No. 59 building 903 Kun Patentee before: Li Xueping |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: Chicken feed probiotic agent containing enterococcus faecalis and preparation method of chicken feed probiotic agent Effective date of registration: 20180319 Granted publication date: 20161116 Pledgee: Beijing ustron Tongsheng financing Company limited by guarantee Pledgor: Beijing Haoshiwo Biological Technology Co., Ltd. Registration number: 2018990000202 |
|
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PC01 | Cancellation of the registration of the contract for pledge of patent right |
Date of cancellation: 20210608 Granted publication date: 20161116 Pledgee: Beijing ustron Tongsheng financing Company limited by guarantee Pledgor: BEIJING HESWOF BIOTECHNOLOGY Co.,Ltd. Registration number: 2018990000202 |
|
PC01 | Cancellation of the registration of the contract for pledge of patent right |