CN103999706A - Method for increasing cutting rooting rate of pecans - Google Patents
Method for increasing cutting rooting rate of pecans Download PDFInfo
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- CN103999706A CN103999706A CN201410286988.2A CN201410286988A CN103999706A CN 103999706 A CN103999706 A CN 103999706A CN 201410286988 A CN201410286988 A CN 201410286988A CN 103999706 A CN103999706 A CN 103999706A
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Abstract
The invention belongs to the technical field of the vegetative propagation and seedling cultivation technique for plants and particularly relates to a method for increasing the cutting rooting rate of pecans. The method for increasing the cutting rooting rate of pecans comprises the steps that a cutting shoot well cut from a seed tree is soaked in rooting powder processing liquid, ultrasonic treatment is conducted on the cutting shoot through an ultrasonic cell crusher at the same time, and then the cutting shoot is inserted into a matrix, wherein the power of the ultrasonic cell crusher ranges from 200 w to 300 w, ultrasonic treatment is conducted for 5 s to 7 s each time, the interval between every two sequential times of ultrasonic treatment is 5 s to 7 s, the total time of ultrasonic treatment is 2 min to 4 min, the temperature of the matrix ranges from 20 DEG C to 28 DEG C, and the water content of the matrix ranges from 20% to 40%. The method for increasing the cutting rooting rate of pecans has the advantages that the time is short, the rooting rate is high, and the number of cottage batches is large.
Description
Technical field
The invention belongs to plant asexual multiplication seedling technical field, be specifically related to a kind of method that improves pecan tree rooting percent.
Background technology
Pecan tree (Carya illinoinensis Koch), has another name called apocarya or pecan, is Juglandaceae hickory plant, one of world-renowned dry fruit tree variety.Pecan tree is introduced a fine variety to China existing more than 110 year, at aspects such as germ plasm resource introduction, fine-variety breeding, multiplication technique, high-yield cultivation techniques, has all accumulated more rich experience, but fails so far to form industrialization, and one of the main reasons is that supply falls short of demand for breeding seedling.Going deep into and promoting along with pecan tree industrial research, the demand of pecan tree seed constantly expands, but the large tree result quantities of domestic pecan tree cannot be satisfied the demand far away, so that international market pecan tree seed price is constantly soaring, and the market price has been soared to the 70-100 unit/kg of 2013 by 20-30 unit/kg several years ago.
In order to accelerate the popularization paces of pecan tree industry, the breeding technology for vigorous seedling cultivation about pecan tree has become study hotspot in recent years.The key of pecan tree strong seedling cultivation is exactly to carry out grafting to obtain with vegetative propagations such as cuttages a large amount of improved seeds nursery stocks that hereditary quality is consistent.Research about pecan tree propagation by grafiting technology is many, has larger contribution, but be all first to cultivate the rootstock seedling that grows directly from seeds to promoting pecan tree seedling growing process, is subject to the impact of seed quality and output larger, and cost is high; Need be through seed-soaking, the husky multiple working procedures such as lamination, vernalization, transplanting of hiding, stock nursery stage from winter then to Second Year August even to the 3rd year spring, the shortcoming such as exist the cycle to grow, have a big risk, labour intensity is large.Therefore, in the urgent need to a kind of Technique on Cuttage Propagation of pecan tree, the large batch of rootstock seedling of quick obtaining, thus reduce pecan tree seedling cost, shorten seedling raise period, and guarantee the merit of stock.
About the research of pecan tree Technique on Cuttage Propagation, mainly contain " apocarya hardwood cuttage method " (application number: 201310022277.X) and Liu Yongzhi of people's inventions such as Wang Zhengjia, " a kind of method that promotes that apocarya branch adventive root occurs " (application number: 201310174751.0) of people's inventions such as Li Yongrong, " a kind of apocarya hardwood cutting and seedling raising method " (application number: 201310247748.7), although the former reaches 80% by rooting rate, but every year can only cuttage in the last ten-days period in March, time is shorter, cuttage batch is lacked, the latter two are that winter to spring all can cuttage, but use heating wire to heat, not only energy consumption is too large, simultaneously because ground hot line is imbedded in moist matrix, when joint is many, wiring is extremely not easy, and easily open circuit, 1-2 just needs to change a heating wire, and troublesome poeration and unstable.Therefore, for obtaining a large amount of cuttage seeding, must extend cutting time, increase on the basis of cuttage lot number, improve cuttage root-taking rate, also be eager to excel simultaneously and adjust low-carbon energy-saving, improve stability and guarantee safety.Utilize solar water heater to heat and just in time guaranteed above some requirement.
Summary of the invention
The object of the present invention is to provide a kind of method that improves pecan tree rooting percent, pecan tree cuttage root-taking method provided by the invention has that the time is short, rooting rate is high, the advantage such as more than cuttage batch.
In order to realize object of the present invention, spy by the following technical solutions:
A method that improves pecan tree rooting percent, comprises the following steps:
When adopting root-inducing powder treatment fluid to soak the cuttings that shears from elite stand, with ultrasonic cell disruption instrument, cuttings is carried out to ultrasonic processing, after finishing by cuttings cuttage to matrix; The power of described ultrasonic cell disruption instrument is 200-300w; The temperature of described matrix is 20-28 ℃, water content of substrate 20%-40%; Preferably, described ultrasonic processing comprises: each ultrasonic 5-7s, and the interval 5-7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 2-4min.Preferred, described ultrasonic processing comprises: each ultrasonic 5-6s, and the interval 5-6s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 3-4min.
The cuttage breeding method of pecan tree cuttings provided by the invention, in control matrix, have under the condition of specific temperature and water content, under the functions of hormones of employing root-inducing powder, be aided with specific ultrasonic processing method simultaneously, greatly irritation cell division, not only greatly reduced the time of taking root, also improved significantly rooting rate simultaneously, can also improve its ability of withstanding natural calamities and promote growth, there is the features such as high, the simple to operate practicality of rooting rate, cost be low, seedling raise period is short, is applicable to extensive cottage propagation.
In addition, for the selection of concrete ultrasonic mode, each ultrasonic time is long, and each ultrasonic time interval is shorter, when adding root-inducing powder hormone, stimulates, and easily causes the destruction to cell; Each ultrasonic time is too short, each hyperacoustic time interval is longer, ultrasonic does not have the fissional effect of ultrasonic stimulation together with root-inducing powder hormone, and the ultrasonic frequency that the present invention selects, ultrasonic time and ultrasonic interval, can stimulate to greatest extent the cell division of cuttings, be conducive to root-inducing powder hormone and enter cell interior, impel formation layer and bundle sheath tissue in branch, form root initial body, thereby development growth goes out adventive root, and form root system, improve rooting rate.
Preferably, by cuttings cuttage to before intramatrical step, also comprise following preparation: at booth, solar water heater is installed outward, steelframe seedbed is placed in booth, on steel wire, lay matrix, sterilization, then lay grid, finally cover matrix, firing equipment, water circulating pump and hot water pipe net are connected into a hot water cyclesystem, water circulating pump plug is connected with temperature controller, the temperature probe of temperature controller is inserted in matrix.The present invention is by a hot water cyclesystem is set, and controls the temperature of matrix and adjusts the temperature in booth, and the root of cuttings can be remained under the temperature condition that optimum takes root always, greatly improved rooting rate, and reduced rootage duration.
Preferably, described firing equipment is solar water heater.When adopting solar water heater as heating source, solar water heater is placed in outside booth.Adopt solar water appliance to have low-carbon energy-saving, improve stability and guarantee the advantages such as safety; When weather conditions affect the efficiency of heating surface of solar water heater, can open the electric heater in solar water heater, can and guarantee on the basis of the advantages such as safety at low-carbon energy-saving, guarantee stability and the continuation of heating.
In order to control better temperature, humidity and the light intensity in booth, preferred, can be outside booth covered with sunshade thing; To after in matrix, keep temperature of shed lower than 18 ℃, slotting machine top air humidity 80%-90% cuttings cuttage; Preferred, keep temperature of shed at 13-18 ℃.The present invention is by adjusting the temperature in booth, humidity, light intensity, make temperature and humidity in booth and temperature and the water content of matrix form a specific gradient, under such gradient, can not germinate in the top of cuttings, upper and lower competition moisture and the nutrition of cuttings have been avoided, can not cause the loss of moisture and nutrition, can impel the bottom of moisture and Nutrition flows cuttings to take root, appearance and the growth of the former base of root of the stimulation pecan tree cuttings that is highly advantageous to, impel educated seedling root luxuriant, greatly improved the cuttage root-taking rate of pecan tree cuttings, and shortened its rootage duration, improved production efficiency.
Preferably, before the cuttings shearing is adopted to the immersion of root-inducing powder treatment fluid, first cuttings is placed under flowing water and is rinsed after 20-30h, then soak 1.5-3h with 40-60%wt acetone from elite stand, the soaking depth of cuttings is 3-5cm.Wherein, 40-60%wt acetone is the mixture of acetone and water.
Preferably, described matrix comprises turfy soil, perlite and vermiculite, and its volume ratio is 4-5:4-5:1.Inventor, through a large amount of orthogonal experiments, constantly changes composition and the content of matrix, finally determines the matrix forming in specific proportions with turfy soil, perlite and vermiculite, has been conducive to shorten the rootage duration of pecan tree cuttings, promotes the breeding of its adventive root.
Preferably, described root-inducing powder treatment fluid is the mixed solution of NAA or IBA or ABT or NAA, IBA and ABT, and the time that adopts root-inducing powder treatment fluid to soak is 2-7h, and in described mixed solution, the mass ratio of NAA, IBA and ABT is 5:2-10:2-25; The concentration of described root-inducing powder treatment fluid is 400-800mg.L
-1.The present invention adopts above-mentioned specific root-inducing powder treatment fluid to coordinate with ultrasonic facture, and the two has the effect of cooperative compensating, can strengthen the schizogamy that stimulates cuttings root cell, and after cuttage 20 days start to take root, and in the time of 40 days, rooting rate reaches more than 62%.
Preferably, the concentration of described root-inducing powder treatment fluid is 400-600mg.L
-1described root-inducing powder treatment fluid is the mixed solution of NAA or NAA, IBA and ABT, and in described mixed solution, the mass ratio of NAA, IBA and ABT is 5:2-3:2-3, and the time that adopts root-inducing powder treatment fluid to soak is 4-7h, after cuttage 20 days start to take root, and in the time of 40 days, rooting rate reaches more than 81%.
Most preferred, the concentration of described root-inducing powder treatment fluid is 450-550mg.L
-1, described root-inducing powder treatment fluid is NAA, and the time that adopts root-inducing powder treatment fluid to soak is 5-6h, and after cuttage 20 days start to take root, and in the time of 40 days, rooting rate reaches more than 86%.
Preferably, described cuttage is directly cuttings to be inserted in mixed-matrix, and seeding row spacing is 3cm * 5cm, and the cuttage degree of depth is 4-5cm, then, by after cuttings matrix compacting around, waters permeable.
Preferably, regularly slotting machine is carried out disinfection, described sterilization is: rotation adopts thiophanate methyl or carbendazol wettable powder to the matrix sterilization that carries out disinfection.By volume, the consumption of thiophanate methyl is thiophanate methyl 750-850 times liquid, the carbendazol wettable powder 550-650 times liquid that the consumption of carbendazol wettable powder is 50%wt.
Compared with prior art, beneficial effect of the present invention is:
(1) cuttage breeding method of pecan tree stock provided by the invention, has that practicality simple to operate, cost are low, rooting rate high, and seedling raise period is short, is applicable to extensive cottage propagation;
(2) the present invention utilizes Scissors used specially for grafting anvil to abandon stock branch for material, by sport technique segments such as temperature control and hormone immersions, nursery stock has been carried out to expansion numerous, turns waste into wealth, and can obtain every year a large amount of high-quality rootstock seedlings, has higher application and popularization value in production;
(3) the present invention is by controlling substrate temperature higher than air themperature in plastic tunnel, extended cuttage season, guaranteed that cuttings germinates after taking root simultaneously, is conducive to the growth of root system, impels educated seedling root luxuriant.
Embodiment
Embodiment 1
(1) slotting machine is prepared
In booth, first ground is flattened, build the steelframe seedbed of wide 0.8-1m, high 0.4-0.8m thereon, then on seedbed, with the high plastic plate of 30cm or plank, surround cuttage pond, two-layer 20 object gauzes in cuttage pond then, the thick mixed-matrix of layer overlay 4-5cm on gauze; Mixed-matrix comprises turfy soil, perlite and vermiculite, and this three's volume ratio is 4:5:1.On mixed-matrix, lay DN20 hot water pipe net, tube pitch is 10-15cm left and right, a connection water circulating pump delivery port of hot-water line, the water inlet of water circulating pump is connected to solar water heater delivery port, the other end of solar water heating pipe connects solar water heater inlet mouth, become path, then on hot water pipe net, spread the mixed-matrix that 15cm is thick, with 650 times of liquid of carbendazol wettable powder of 50%wt to the matrix sterilization that carries out disinfection, and flatten stand-by, by water circulating pump plug be connected with temperature controller, the temperature probe of temperature controller is inserted in plugging soil, switch on power, control slotting machine temperature at 20 ℃, and set solar heat coolant-temperature gage more than 50 ℃, guarantee as the moon, rainy day is in the time of cannot utilizing solar energy, can automatically carry out electrical heating, guarantee the transmission of slotting machine heat.
(2) cuttings collection
Clip 10cm health, full 1 year raw branch from scion-seed tree, upper cut straight snips, lower cut is tiltedly cut apart from the about 0.2cm of bud.
(3) cuttings is processed
The cuttings shearing is placed under flowing water and is rinsed after 24h, then soak 2h, soaking depth 3-4cm with 50%wt acetone; Then by concentration, be 450mg.L
-1nAA root-inducing powder treatment fluid soak cuttings 6h, soaking depth 3-4cm.When root-inducing powder is processed, with supersonic generator, cuttings is carried out to ultrasonic processing, ultrasonic power is 200w, each ultrasonic 7s, the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 2min.
(4) cottage method
Cuttage is directly cuttings to be inserted in mixed-matrix, and seeding row spacing is 2-4cm * 4-5cm, and the cuttage degree of depth is 4-5cm, then by cuttings matrix compacting around, and irrigates immediately water.Each processes cuttage 100 strains, 3 repetitions.
(5) insert rear management
At booth, cover the sunshade net that one deck shading rate is 70%-90% outward, and according to temp. and humidity situation in booth to carrying out vaporific water spray in canopy, keep temperature of shed at 13 ℃, slotting machine top air humidity 80%, prevents that cuttings dehydration is withered; Keep 20 ℃ of slotting machine substrate temperature, water content of substrate 20%; Every 7 days, slotting machine is carried out disinfection with 600 times of liquid of carbendazol wettable powder of 50%wt, within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 86.33%, 3.2 of average indefinite radicals, the long 6cm of the longest adventive root.
Embodiment 2
The present embodiment adopts the method for embodiment 1, and the difference of embodiment 1 is: the temperature of controlling matrix is 25 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L
-1, adopt root-inducing powder treatment fluid to soak cuttings 5.5h.Described ultrasonic processing comprises: each ultrasonic 6s, and the interval 6s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 3min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 91.67%.3.4 of average indefinite radicals, the long 15.5cm of the longest adventive root.
Embodiment 3
The present embodiment adopts the method for embodiment 1, and the difference of embodiment 1 is: the temperature of controlling matrix is 28 ℃, water content of substrate 40%.The concentration of root-inducing powder treatment fluid is 550mg.L
-1, adopt root-inducing powder treatment fluid to soak cuttings 5h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 5s, and the interval 5s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 88.33%.3.3 of average indefinite radicals, the long 5.3cm of the longest adventive root.
Embodiment 4
(1) slotting machine is prepared
In booth, first ground is flattened, build the steelframe seedbed of wide 0.8-1m, high 0.4-0.8m thereon, then on seedbed, with the high plastic plate of 30cm or plank, surround cuttage pond, two-layer 20 object gauzes in cuttage pond then, the thick mixed-matrix of layer overlay 4-5cm on gauze; Mixed-matrix comprises turfy soil, perlite and vermiculite, and this three's volume ratio is 5:4:1.On mixed-matrix, lay DN20 hot water pipe net, tube pitch is 12cm left and right, a connection water circulating pump delivery port of hot-water line, the water inlet of water circulating pump is connected to solar water heater delivery port, the other end of solar water heating pipe connects solar water heater inlet mouth, become path, then on hot water pipe net, spread the mixed-matrix that 10-15cm is thick, with 600 times of liquid of carbendazol wettable powder of 50%wt to the matrix sterilization that carries out disinfection, and flatten stand-by, by water circulating pump plug be connected with temperature controller, the temperature probe of temperature controller is inserted in plugging soil, switch on power, control slotting machine temperature between 25 ℃, and set solar heat coolant-temperature gage more than 50 ℃, guarantee as the moon, rainy day is in the time of cannot utilizing solar energy, can automatically carry out electrical heating, guarantee the transmission of slotting machine heat.
(2) cuttings collection
Clip 10cm health, full 1 year raw branch from scion-seed tree, upper cut straight snips, lower cut is tiltedly cut apart from the about 0.2cm of bud.
(3) cuttings is processed
The cuttings shearing is placed under flowing water and is rinsed after 30h, then soak 1.5h, soaking depth 3-5cm with 60%wt acetone; Then by concentration, be 500mg.L
-1nAA, IBA and the mixed solution of ABT soak cuttings 7h, wherein, the mass ratio of NAA, IBA and ABT is 5:3:2.When root-inducing powder is processed, with supersonic generator, cuttings is carried out to ultrasonic processing, ultrasonic power is 200w, each ultrasonic 7s, the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.
(4) cottage method
Cuttage is directly cuttings to be inserted in mixed-matrix, and seeding row spacing is 2-4cm * 4-6cm, and the cuttage degree of depth is 4-5cm, then by cuttings matrix compacting around, and irrigates immediately water.Each processes cuttage 100 strains, 3 repetitions.
(5) insert rear management
At booth, cover the sunshade net that one deck shading rate is 70%-90% outward, and according to temp. and humidity situation in booth to carrying out vaporific water spray in canopy, keep temperature of shed at 18 ℃, slotting machine top air humidity 90%, prevents that cuttings dehydration is withered; Keep 25 ℃ of slotting machine substrate temperature, water content of substrate 40%; Every 7 days, slotting machine is carried out disinfection with 800 times of liquid of sterling thiophanate methyl, within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 81.67%.2.8 of average indefinite radicals, the long 10.2cm of the longest adventive root.
Embodiment 5
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 400mg.L
-1, wherein, the mass ratio of NAA, IBA and ABT is 5:2:3, adopts root-inducing powder treatment fluid to soak cuttings 7h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 82.66%.2.5 of average indefinite radicals, the long 8.2cm of the longest adventive root.
Embodiment 6
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 800mg.L
-1nAA liquid, adopt root-inducing powder treatment fluid to soak cuttings 2h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 64.33%.2 of average indefinite radicals, the long 4.8cm of the longest adventive root.
Embodiment 7
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L
-1iBA liquid, adopt root-inducing powder treatment fluid to soak cuttings 6h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 62.33%.2 of average indefinite radicals, the long 6.9cm of the longest adventive root.
Embodiment 8
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L
-1aBT liquid, adopt root-inducing powder treatment fluid to soak cuttings 6h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 68.33%.2.2 of average indefinite radicals, the long 10.1cm of the longest adventive root.
Embodiment 9
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L
-1, wherein, the mass ratio of NAA, IBA and ABT is 5:10:25, adopts root-inducing powder treatment fluid to soak cuttings 6h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 68.66%.2 of average indefinite radicals, the long 6.9cm of the longest adventive root.
Embodiment 10
The present embodiment adopts the method for embodiment 4, and the difference of embodiment 4 is: the temperature of controlling matrix is 28 ℃, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L
-1, wherein, the mass ratio of NAA, IBA and ABT is 5:2:2, adopts root-inducing powder treatment fluid to soak cuttings 7h.Described ultrasonic processing comprises: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 4min.Within after inserting 7-10 days, produce callus, 20 days start to take root, cuttage after 40 days rooting rate reach 81.67%.2.3 of average indefinite radicals, the long 7.3cm of the longest adventive root.
Test example 1
The impact of the rooting method of the rooting method that this test example has compared the embodiment of the present invention and corresponding comparative group on pecan tree rooting percent.
Comparative group 1-10 adopts respectively the rooting method of the embodiment 1-10 corresponding with its numbering, and difference is that the sample in comparative group does not all adopt ultrasonic processing.
Clear water control group adopts the rooting method of embodiment 1, and difference is: do not adopt root-inducing powder treatment fluid to soak, and adopt clear water to soak 6h, adopt ultrasonic to process and do not adopt the rooting rate after ultrasonic is processed to be 0.
Table 1 hormon and the impact of ultrasonic processing on pecan tree cuttage root-taking rate
| ? | The rooting rate of embodiment | The rooting rate of comparative example |
| Numbering 1 | 86.33％ | 57.0％ |
| Numbering 2 | 91.67％ | 58.33％ |
| Numbering 3 | 88.33％ | 57.66％ |
| Numbering 4 | 81.67％ | 52.67％ |
| Numbering 5 | 82.66％ | 53％ |
| Numbering 6 | 64.33％ | 47.67％ |
| Numbering 7 | 62.33％ | 31.11％ |
| Numbering 8 | 68.33％ | 38.67％ |
| Numbering 9 | 68.66％ | 46％ |
| Numbering 10 | 81.67％ | 52.67％ |
As can be seen from Table 1: after ultrasonic processing, pecan tree rooting percent can improve 16.66%-33.34%.The rooting percent of different root-growing agent combined treatment changes along with the difference of hormone concentration and soak time.When adopting concentration, be 450-550mg.L
-1nAA root-inducing powder treatment fluid soak 5-6 hour, and process in conjunction with ultrasonic, rooting rate reaches more than 86%.When adopting concentration, be 400-600mg.L
-1nAA or the mixed solution of NAA, IBA and ABT as root-inducing powder treatment fluid, soak 4-7 hour, in conjunction with ultrasonic, process, rooting rate reaches more than 81%.When adopting concentration, be 400-800mg.L
-1nAA or the mixed solution of IBA or ABT or NAA, IBA and ABT as root-inducing powder treatment fluid, soak 2-7 hour, in conjunction with ultrasonic, process, rooting rate reaches more than 62%.And while soaking 2h with clear water, no matter whether pass through ultrasonic processing, cuttings all cannot be taken root in hotbed.
Claims (10)
1. a method that improves pecan tree rooting percent, is characterized in that, comprises the following steps:
When adopting root-inducing powder treatment fluid to soak the cuttings that shears from elite stand, with ultrasonic cell disruption instrument, cuttings is carried out to ultrasonic processing, after finishing by cuttings cuttage to matrix; The power of described ultrasonic cell disruption instrument is 200-300w; Described ultrasonic processing comprises: each ultrasonic 5-7s, and the interval 5-7s of adjacent twice ultrasonic processing, the total time of ultrasonic processing is 2-4min; The temperature of described matrix is 20-28 ℃, water content of substrate 20%-40%.
2. method according to claim 1, it is characterized in that, by cuttings cuttage to before intramatrical step, also comprise following preparation: at booth, solar water heater is installed outward, steelframe seedbed is placed in booth, on steel wire, lay matrix, sterilization, then lays grid, finally cover matrix, firing equipment, water circulating pump and hot water pipe net are connected into a hot water cyclesystem, water circulating pump plug is connected with temperature controller, the temperature probe of temperature controller is inserted in matrix.
3. method according to claim 2, is characterized in that, described firing equipment is solar water heater.
4. method according to claim 2, is characterized in that, to after in matrix, keeps temperature of shed lower than 18 ℃, slotting machine top air humidity 80%-90% cuttings cuttage; Preferably, keep temperature of shed at 13-18 ℃.
5. method according to claim 1, it is characterized in that, before the cuttings shearing is adopted to the immersion of root-inducing powder treatment fluid, first cuttings is placed under flowing water and is rinsed after 20-30h from elite stand, with 40-60%wt acetone, soak 1.5-3h again, the soaking depth of cuttings is 3-5cm.
6. method according to claim 1, is characterized in that, described matrix comprises turfy soil, perlite and vermiculite, and its volume ratio is 4-5:4-5:1.
7. method according to claim 1, it is characterized in that, described root-inducing powder treatment fluid is the mixed solution of NAA or IBA or ABT or NAA, IBA and ABT, the time that adopts root-inducing powder treatment fluid to soak is 2-7h, and in described mixed solution, the mass ratio of NAA, IBA and ABT is 5:3-10:2-25; The concentration of described root-inducing powder treatment fluid is 400-800mg.L
-1.
8. method according to claim 7, is characterized in that, the concentration of described root-inducing powder treatment fluid is 400-600mg.L
-1, described root-inducing powder treatment fluid is the mixed solution of NAA or NAA, IBA and ABT, and in described mixed solution, the mass ratio of NAA, IBA and ABT is 5:2-3:2-3, and the time that adopts root-inducing powder treatment fluid to soak is 4-7h; Preferably, the concentration of described root-inducing powder treatment fluid is 450-550mg.L
-1, described root-inducing powder treatment fluid is NAA, the time that adopts root-inducing powder treatment fluid to soak is 5-6h.
9. method according to claim 1, is characterized in that, described cuttage is directly cuttings to be inserted in mixed-matrix, and seeding row spacing is 2-4cm * 4-6cm, and the cuttage degree of depth is 4-5cm, then, by after cuttings matrix compacting around, waters permeable.
10. method according to claim 4, is characterized in that, regularly slotting machine is carried out disinfection, and described sterilization is: rotation adopts thiophanate methyl or carbendazol wettable powder to the matrix sterilization that carries out disinfection.
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| CN201410286988.2A CN103999706B (en) | 2014-06-24 | 2014-06-24 | A kind of method improving pecan tree rooting percent |
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| CN103999706B CN103999706B (en) | 2017-01-04 |
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| CN104521672A (en) * | 2014-12-24 | 2015-04-22 | 普定县四海农业发展有限公司 | Method for planting walnut trees |
| CN104823790A (en) * | 2015-04-29 | 2015-08-12 | 河池学院 | Rapid juglans sigillata cutting propagation method |
| CN104945139A (en) * | 2015-06-26 | 2015-09-30 | 江苏水木农景股份有限公司 | Substrate for culturing pecans and method for preparing the same |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106342582A (en) * | 2016-08-25 | 2017-01-25 | 合肥市田然农业科技园有限公司 | High-quality chrysanthemum flower planting method |
| CN106577237A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Method of cutting in winter for saniodendron subaequalum |
| CN106718536A (en) * | 2016-12-01 | 2017-05-31 | 南京绿宙薄壳山核桃科技有限公司 | It is a kind of to heat the method that bed carries out apocarya cuttage using fermentation |
| CN106718515A (en) * | 2016-11-26 | 2017-05-31 | 防城港市林木良种繁育中心苗圃 | A kind of method for accelerating the germination of cuttage banyan |
| CN107223506A (en) * | 2017-05-25 | 2017-10-03 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of thin skin hickory nut |
| CN107251797A (en) * | 2017-06-19 | 2017-10-17 | 四川森迪科技发展股份有限公司 | A kind of fig tree method for fast asexual propagation |
| CN107624394A (en) * | 2017-08-24 | 2018-01-26 | 浦江县合洪园艺研发有限公司 | A kind of method for inducing hickory nut adventitious root to occur |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN110637622A (en) * | 2019-11-05 | 2020-01-03 | 中国林业科学研究院亚热带林业实验中心 | Method for asexual cuttage propagation of vernicia fordii |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
| CN113303114A (en) * | 2021-05-18 | 2021-08-27 | 郑州市农林科学研究所 | Method for reducing total tannin content of cyclobalanopsis glauca cutting slips |
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Cited By (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104521672A (en) * | 2014-12-24 | 2015-04-22 | 普定县四海农业发展有限公司 | Method for planting walnut trees |
| CN104823790B (en) * | 2015-04-29 | 2018-03-20 | 河池学院 | A kind of bulla walnut cuttage fast seedling-cultivating method |
| CN104823790A (en) * | 2015-04-29 | 2015-08-12 | 河池学院 | Rapid juglans sigillata cutting propagation method |
| CN104945139A (en) * | 2015-06-26 | 2015-09-30 | 江苏水木农景股份有限公司 | Substrate for culturing pecans and method for preparing the same |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106342582A (en) * | 2016-08-25 | 2017-01-25 | 合肥市田然农业科技园有限公司 | High-quality chrysanthemum flower planting method |
| CN106718515A (en) * | 2016-11-26 | 2017-05-31 | 防城港市林木良种繁育中心苗圃 | A kind of method for accelerating the germination of cuttage banyan |
| CN106718536A (en) * | 2016-12-01 | 2017-05-31 | 南京绿宙薄壳山核桃科技有限公司 | It is a kind of to heat the method that bed carries out apocarya cuttage using fermentation |
| CN106577237A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Method of cutting in winter for saniodendron subaequalum |
| CN107223506A (en) * | 2017-05-25 | 2017-10-03 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of thin skin hickory nut |
| CN107251797A (en) * | 2017-06-19 | 2017-10-17 | 四川森迪科技发展股份有限公司 | A kind of fig tree method for fast asexual propagation |
| CN107624394A (en) * | 2017-08-24 | 2018-01-26 | 浦江县合洪园艺研发有限公司 | A kind of method for inducing hickory nut adventitious root to occur |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN110637622A (en) * | 2019-11-05 | 2020-01-03 | 中国林业科学研究院亚热带林业实验中心 | Method for asexual cuttage propagation of vernicia fordii |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
| CN113303114A (en) * | 2021-05-18 | 2021-08-27 | 郑州市农林科学研究所 | Method for reducing total tannin content of cyclobalanopsis glauca cutting slips |
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Application publication date: 20140827 Assignee: Beijing Huamei Wanxiang Technology Co., Ltd. Assignor: Nanjing Forestry University Contract record no.: 2018320000235 Denomination of invention: Method for increasing cutting rooting rate of pecans Granted publication date: 20170104 License type: Common License Record date: 20181024 Application publication date: 20140827 Assignee: Nanjing city Pukou District moon farm Assignor: Nanjing Forestry University Contract record no.: 2018320000234 Denomination of invention: Method for increasing cutting rooting rate of pecans Granted publication date: 20170104 License type: Common License Record date: 20181024 |
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