CN101743829A - Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment - Google Patents
Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment Download PDFInfo
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- CN101743829A CN101743829A CN200910175340A CN200910175340A CN101743829A CN 101743829 A CN101743829 A CN 101743829A CN 200910175340 A CN200910175340 A CN 200910175340A CN 200910175340 A CN200910175340 A CN 200910175340A CN 101743829 A CN101743829 A CN 101743829A
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000004083 survival effect Effects 0.000 title claims abstract description 11
- 238000009210 therapy by ultrasound Methods 0.000 title abstract description 3
- 238000007598 dipping method Methods 0.000 claims abstract description 8
- 238000011282 treatment Methods 0.000 claims abstract description 7
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims abstract description 4
- 241000238631 Hexapoda Species 0.000 claims abstract description 4
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000006013 carbendazim Substances 0.000 claims abstract description 4
- 238000009333 weeding Methods 0.000 claims abstract description 4
- 241000607479 Yersinia pestis Species 0.000 claims abstract description 3
- 239000011159 matrix material Substances 0.000 claims description 13
- 238000012545 processing Methods 0.000 claims description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 239000007788 liquid Substances 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 3
- 239000000243 solution Substances 0.000 claims description 3
- 238000004659 sterilization and disinfection Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 2
- 238000010790 dilution Methods 0.000 claims description 2
- 230000001976 improved effect Effects 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract description 7
- 201000010099 disease Diseases 0.000 abstract description 2
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- 239000002689 soil Substances 0.000 abstract 2
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 241000196324 Embryophyta Species 0.000 description 14
- 230000000694 effects Effects 0.000 description 12
- 102000018997 Growth Hormone Human genes 0.000 description 10
- 108010051696 Growth Hormone Proteins 0.000 description 10
- 239000000122 growth hormone Substances 0.000 description 8
- 239000005556 hormone Substances 0.000 description 8
- 229940088597 hormone Drugs 0.000 description 8
- 230000010496 root system development Effects 0.000 description 8
- 241000124033 Salix Species 0.000 description 7
- 241000249899 Populus tomentosa Species 0.000 description 6
- 210000005069 ears Anatomy 0.000 description 4
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- 230000035699 permeability Effects 0.000 description 2
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- 230000021749 root development Effects 0.000 description 2
- QJZYHAIUNVAGQP-UHFFFAOYSA-N 3-nitrobicyclo[2.2.1]hept-5-ene-2,3-dicarboxylic acid Chemical compound C1C2C=CC1C(C(=O)O)C2(C(O)=O)[N+]([O-])=O QJZYHAIUNVAGQP-UHFFFAOYSA-N 0.000 description 1
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- 238000004945 emulsification Methods 0.000 description 1
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Abstract
A method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment aims at realizing simple operation and capability of treating large quantity of cuttage. The method of the invention comprises: clipping strong 1 to 2-year-old branches of woody plant without disease and insect pest, and bundling up the clipped branches; placing the clipped branch bundle into ABT solution, and dipping with a treatment method of dipping root; treating the clipped branches with a ultrasonic wave generating unit, wherein the ultrasonic intensity is 200W to 400W, and the treating time is 30s to 60s; timely watering and weeding after cutting; preparing a seedbed, and laying base material and garden soil which are mixed on the bottom of the seedbed, wherein the weight percent of the base material and the garden soil is 50 percent; sterilizing the base material with 500 times solution diluted by 30 percent of carbendazim; watering the seedbed thoroughly; cutting after 2 to 3h when the treatment of the base material is completed; and sticking the treated branches in the prepared seedbed.
Description
Technical field
The present invention relates to a kind ofly handle the new method that shrub woody plant improves its survival rate and promotes root system development jointly with ultrasonic and hormone
Technical background
In the vegetative propagation of trees, mainly be several modes of nourishing and generating such as cuttage, grafting and tissue culture at present. from the economy and the easy angle of technology, cottage propagation is comparatively ideal technology.In the cuttage process, taking root of plugged ear not only is subjected to the influence of endogenous hormones, and be subjected to the adjusting of exogenous hormone, this is because exogenous growth hormone can provide the required growth helping matter of taking root for plugged ear, promote the plugged ear endogenous hormones synthetic, and can promote a root original hase to be differentiated to form a plurality of tips of a root, to induce the morphogenesis of cutting adventive root.So promote that traditionally the method for cuttage root-taking generally is to improve the rooting rate and the root system development situation of plugged ear by the way of using exogenous growth hormone to handle plugged ear, the exogenous hormone that uses on producing generally has NAA, IAA, IBA and ABT (root-inducing powder) etc., they are the effect of its promotion cuttage root-taking of competence exertion under suitable concentration, soak time condition only, otherwise be difficult to show its effect, even also have negative interaction, and also be not quite similar for the root effect of urging of different plant plugged ear exogenous growth hormones.Ultrasonic has cavitation, and suitable ultrasonic can produce emulsification or activate bubble when propagating in liquid, and the structural form of material molecule is changed. and increase the permeability of cell membrane, cell wall, be beneficial to the interchange of the inside and outside material of born of the same parents.Willow is the very wide fast growing tree of a kind of planting range, also is to study the idealized model plant of how to improve arbor woody plant cuttage survival rate simultaneously.
Summary of the invention
The objective of the invention is for overcoming the deficiency of above-mentioned prior art, is model plant with the willow, provide a kind of simple to operate and once can handle a large amount of plugged ears handle to improve the method for shrub woody plant cuttage root-taking and survival rate with ultrasonic.
The present invention adopts ultrasonic and somatotropin to handle the plant plugged ear jointly, and concrete technical scheme is:
(1) spring the leaf bud smooth surface, still do not have greenery and period occurs, 1～2 year living branch of the healthy and strong anosis insect pest woody plant of clip is bundled into wisp with the plugged ear that shears.Branch length 10～15cm is with 2～3 buds to be advisable;
(2) will cut the plugged ear that cuts in ABT (root-inducing powder) solution, adopt the processing mode of dipping in root to soak;
(3) adopt ultrasonic generator to handle plugged ear, ultrasonic intensity is 200W-400W, and the processing time is 30s-60s; In time water and weeding after the cuttage;
(4) be ready to the seedbed, mix being laid on the bottom, seedbed earlier with matrix and garden mould, matrix and garden mould weight respectively account for 50%; With 500 times of liquid of 30% carbendazim dilution matrix is carried out disinfection; Water irrigates the seedbed; 2～3h carries out cuttage after the matrix treatments; With the branch cutting handled on ready seedbed.
The plugged ear of woody plant of the present invention is the willow plugged ear.Choosing has the plugged ear of 2～3 leaf buds, in the leaf bud smooth surface, does not still have to advance when greenery occur plugged ear is handled, because the leaf bud of this moment is in cellular metabolism vigorous period, can synthesize endogenous growth hormone in a large number.
Adopt the 200W-400W ultrasonic intensity to handle plugged ear, divides that to carry out effect for 7 times relatively good, the working time of each processing is 7s, and be 7s blanking time.
The present invention has great importance to the woody plant of the difficulty of taking root, regeneration period length, particularly the method that can utilize ultrasonic and hormone to handle plugged ear jointly for those precious seeds improves the plugged ear survival rate, thereby solves the seedling propagation difficult problem of rare tree.Operating technology is comparatively simple, and the unskilled labor author just can operate, and once can handle a large amount of plugged ears.Utilize ultrasonic can increase the effect of cell permeability, adopt the ultrasonic processing willow plugged ear of suitable power just might produce certain spread effect to it, thereby help exogenous growth hormone and enter cell interior, induce the differentiation of root original hase and the morphogenesis of adventive root, improve early stage growth rate after its root of hair rate, transplanting survival rate and the cuttage.Through experiment confirm, adopt ultrasonic and somatotropin to handle the plugged ear of woody plant jointly after, compare with only adopting HORMONE TREATMENT or be left intact, improved the rooting rate and the root system development situation of woody plant plugged ear greatly.The present invention has carried out test and has obtained success on willow, the present invention provides efficient, an economic approach for the cuttage root-taking of arbor woody plant, particularly willow.
Embodiment
Material therefor of the present invention is Chinese white poplar (Populus tomentosa Carr), is provided by Shanxi Province Academy of Agricultural Sciences biotech research center.1～2 year livings lignification branch choosing healthy and strong no damage by disease and insect in early and middle ten days in March (local different weather conditions also different) is cut section, and plugged ear is about 10～15cm to be grown, and is advisable to be with 2～3 buds.The plugged ear that shears is bundled into wisp.Mix to be laid on the bottom, seedbed with matrix and garden mould, matrix accounts for 50%, and the effect of the insulation that this matrix tool is good, heat insulation, ventilation, water conservation, guarantor's fertilizer is good cutting medium, with 500 times of liquid of 30% carbendazim matrix is carried out disinfection again.First water irrigates the seedbed before the cuttage, and 2～3h carries out cuttage after the matrix treatments.Experimental field be located at the green house of Shanxi Province Academy of Agricultural Sciences biotech research center.
With different hormones (IAA, NAA and ABT) with soak duration (dipping in root, 6h and 12h) two factors and handle plugged ear determining relatively more suitable somatotropin and to soak duration, by the ultrasonic Processing Test that trial test determines to adopt ABT solution, the processing mode of dipping in root is carried out next step.With growth hormone is ABT, soaks duration for dipping in root as quantitatively, adopts 3 kinds of ultrasonic intensities (200W, 400W and 600W) to handle plugged ear respectively, 3 repetitions, and every processing 30 strain plugged ears contrast the totally 90 strain plugged ears that are left intact.Note after the cuttage observing, in time water and weeding.The rooting rate of statistics plugged ear, take root number, root length and the longest root length after 30 days.
ABT (root-inducing powder) is produced by Anhui Province inaction humic acid factory.Ningbo new sesame JY92-II type ultrasonic cell disruptor is adopted in experiment.Except that the intensity difference, remaining parameter was all identical during ultrasonic was handled.Promptly in three kinds of different ultrasonic intensities, the work number of times is 7 times.Each working time is 7s, is 7s blanking time.
Use ultrasonic to handle as can be seen from Table 1 to the influence of Chinese white poplar rooting percent and root growth and development situation, after Chinese white poplar being used the ultrasonic processing of suitable intensity (this research is 400W), the rooting rate of plugged ear and root system development situation have all obviously surpassed does not use hyperacoustic processing (promptly only use growth hormone handle and be left intact), and it is carried out the result of multiple ratio after shows, difference between various different disposal is extremely remarkable, so, select the ultrasonic of suitable power to handle (this research is 400W) to promoting willow and take root and root system development having good effect.
As can be seen from Table 1, be ABT with growth hormone, soak duration for dipping in root as quantitatively, and use simultaneously under the ultrasonic processing Chinese white poplar plugged ear condition, different ultrasonic between Chinese white poplar rooting rate and the root system development situation to influence difference extremely remarkable.It is carried out multiple comparative analysis learn, best with 200W and 400W ultrasonic treatment effect, rooting rate is respectively 90%T and 100%, and the root system development situation is also good, and short root effect is apparently higher than contrast, the two with contrast between difference extremely remarkable.The treatment effect of 600W is the poorest, and rooting rate only has 20%, and the root system development situation is the poorest, and effect significantly is lower than contrast.
Claims (3)
1. one kind handle to be improved the method for shrub woody plant cuttage root-taking and survival rate with ultrasonic, it is characterized in that:
(1) spring the leaf bud smooth surface, still do not have greenery and period occurs, 1～2 year living branch of the healthy and strong anosis insect pest woody plant of clip is bundled into wisp with the plugged ear that shears;
(2) will cut the plugged ear that cuts in ABT solution, adopt the processing mode of dipping in root to soak;
(3) adopt ultrasonic generator to handle plugged ear, ultrasonic intensity is 200W-400W, and the processing time is 30s-60s; In time water and weeding after the cuttage;
(4) be ready to the seedbed, mix being laid on the bottom, seedbed earlier with matrix and garden mould, matrix and garden mould weight respectively account for 50%; With 500 times of liquid of 30% carbendazim dilution matrix is carried out disinfection; Water irrigates the seedbed; 2～3h carries out cuttage after the matrix treatments; With the branch cutting handled on ready seedbed.
2. the method with ultrasonic processing raising shrub woody plant cuttage root-taking and survival rate as claimed in claim 1 is characterized in that branch length 10～15cm, is with 2～3 buds to be advisable.
3. the method that improves shrub woody plant cuttage root-taking and survival rate of handling with ultrasonic as claimed in claim 1, it is characterized in that adopting the 200W-400W ultrasonic intensity to handle plugged ear, divide and carry out for 7 times, each working time of handling is 7s, and be 7s blanking time.
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| Application Number | Priority Date | Filing Date | Title |
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| CN200910175340A CN101743829A (en) | 2009-12-08 | 2009-12-08 | Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment |
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| CN200910175340A CN101743829A (en) | 2009-12-08 | 2009-12-08 | Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment |
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Cited By (16)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960190A (en) * | 2012-11-30 | 2013-03-13 | 赵永圣 | Ultrasonic plant growth-promoting and yield-increasing machine |
| CN103999706A (en) * | 2014-06-24 | 2014-08-27 | 南京林业大学 | Method for increasing cutting rooting rate of pecans |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN105830716A (en) * | 2016-04-08 | 2016-08-10 | 常州润达科技信息咨询有限公司 | Cutting seedling raising method for Antiaris toxicaria |
| CN106171742A (en) * | 2016-07-13 | 2016-12-07 | 阜南县猛发工艺品有限公司 | A kind of implantation methods of the purple willow for braiding |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106576781A (en) * | 2016-11-30 | 2017-04-26 | 枞阳县横山生态农业有限公司 | Cuttage seedling-culturing method of cape jasmine flower |
| CN106665267A (en) * | 2016-11-26 | 2017-05-17 | 阜阳金满地生态农业科技股份有限公司 | Cultivation method of drought resistant grape cutting seedlings |
| CN106718515A (en) * | 2016-11-26 | 2017-05-31 | 防城港市林木良种繁育中心苗圃 | A kind of method for accelerating the germination of cuttage banyan |
| CN107231998A (en) * | 2017-05-27 | 2017-10-10 | 马鞍山市永辉园林绿化有限公司 | A kind of cuttage and seedling culture method of trident maple |
| CN107409890A (en) * | 2017-03-23 | 2017-12-01 | 杨军 | A kind of processing method of willow cutting |
| CN107455225A (en) * | 2017-09-06 | 2017-12-12 | 河南省农村科学技术开发中心有限责任公司 | A kind of cutting propagation root promoting method of crape myrtle |
| CN107950573A (en) * | 2017-12-20 | 2018-04-24 | 马鞍山市双飞葡萄专业合作社 | A kind of grape cuttage bar processing method for improving grape seed procyanidine content |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN109220280A (en) * | 2017-08-24 | 2019-01-18 | 中国科学院寒区旱区环境与工程研究所 | Grafting method for promoting asexual propagation of populus euphratica |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
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2009
- 2009-12-08 CN CN200910175340A patent/CN101743829A/en active Pending
Cited By (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102960190A (en) * | 2012-11-30 | 2013-03-13 | 赵永圣 | Ultrasonic plant growth-promoting and yield-increasing machine |
| CN103999706A (en) * | 2014-06-24 | 2014-08-27 | 南京林业大学 | Method for increasing cutting rooting rate of pecans |
| CN103999706B (en) * | 2014-06-24 | 2017-01-04 | 南京林业大学 | A kind of method improving pecan tree rooting percent |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
| CN105830716A (en) * | 2016-04-08 | 2016-08-10 | 常州润达科技信息咨询有限公司 | Cutting seedling raising method for Antiaris toxicaria |
| CN106171742A (en) * | 2016-07-13 | 2016-12-07 | 阜南县猛发工艺品有限公司 | A kind of implantation methods of the purple willow for braiding |
| CN106305035A (en) * | 2016-07-22 | 2017-01-11 | 阜南县永盛工艺品有限公司 | Rapid cutting seedling raising method for hedera helix |
| CN106665267A (en) * | 2016-11-26 | 2017-05-17 | 阜阳金满地生态农业科技股份有限公司 | Cultivation method of drought resistant grape cutting seedlings |
| CN106718515A (en) * | 2016-11-26 | 2017-05-31 | 防城港市林木良种繁育中心苗圃 | A kind of method for accelerating the germination of cuttage banyan |
| CN106576781A (en) * | 2016-11-30 | 2017-04-26 | 枞阳县横山生态农业有限公司 | Cuttage seedling-culturing method of cape jasmine flower |
| CN107409890A (en) * | 2017-03-23 | 2017-12-01 | 杨军 | A kind of processing method of willow cutting |
| CN107231998A (en) * | 2017-05-27 | 2017-10-10 | 马鞍山市永辉园林绿化有限公司 | A kind of cuttage and seedling culture method of trident maple |
| CN109220280A (en) * | 2017-08-24 | 2019-01-18 | 中国科学院寒区旱区环境与工程研究所 | Grafting method for promoting asexual propagation of populus euphratica |
| CN107455225A (en) * | 2017-09-06 | 2017-12-12 | 河南省农村科学技术开发中心有限责任公司 | A kind of cutting propagation root promoting method of crape myrtle |
| CN107950573A (en) * | 2017-12-20 | 2018-04-24 | 马鞍山市双飞葡萄专业合作社 | A kind of grape cuttage bar processing method for improving grape seed procyanidine content |
| CN107950573B (en) * | 2017-12-20 | 2021-01-22 | 安徽省松邦农业科技有限公司 | Grape cutting processing method for improving procyanidine content of grapes |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
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