CN103999706B - A kind of method improving pecan tree rooting percent - Google Patents
A kind of method improving pecan tree rooting percent Download PDFInfo
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- CN103999706B CN103999706B CN201410286988.2A CN201410286988A CN103999706B CN 103999706 B CN103999706 B CN 103999706B CN 201410286988 A CN201410286988 A CN 201410286988A CN 103999706 B CN103999706 B CN 103999706B
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Abstract
The invention belongs to plant asexual multiplication seedling technical field, be specifically related to a kind of method improving pecan tree rooting percent.The method comprises the following steps: the cutting sheared on elite stand uses root-inducing powder treatment fluid with ultrasonic cell disruption instrument, cutting is carried out supersound process, by cutting cuttage to Medium Culture after terminating while soaking；The power of described ultrasonic cell disruption instrument is 200 300w；Described supersound process includes: the most ultrasonic 5 7s, interval 5 7s of adjacent twice supersound process, and the total time of supersound process is 2 4min；The temperature of described substrate is 20 28 DEG C, water content of substrate 20% 40%.The pecan tree cuttage root-taking method that the present invention provides has the advantages such as the time is short, rooting rate is high, cuttage batch is many.
Description
Technical field
The invention belongs to plant asexual multiplication seedling technical field, be specifically related to a kind of raising pecan tree cuttage rooting
The method of rate.
Background technology
Pecan tree (Carya illinoinensis Koch), has another name called apocarya or pecan, is Semen Juglandis
Section hickory plant, one of world-renowned dry fruit tree variety.Pecan tree is introduced a fine variety to China existing more than 110 year, in kind of a matter
The aspects such as resource introduction, fine-variety breeding, multiplication technique, high-yield cultivation technique all have accumulated more rich experience, but fails so far
Forming industrialization, one of the main reasons is that supply falls short of demand for breeding Seedling.Going deep into and promoting along with pecan tree industrial research, beautiful
The demand of state's Semen Caryae Cathayensis seed constantly expands, but domestic pecan tree big tree result quantities cannot meet far away needs, so that
International market pecan tree seed price constantly rises, and the market price is soared to 2013 by 20-30 unit/kg several years ago
70-100 unit/kg.
In order to accelerate the popularization paces of pecan tree industry, in recent years about the breeding strong seedling cultivation skill of pecan tree
Art has become study hotspot.The key of pecan tree strong seedling cultivation is just by the asexual propagation such as grafting and cuttage and obtains heredity
A large amount of improved seeds nursery stocks of uniform quality.Research about pecan tree propagation by grafiting technology is the most, to promoting the U.S.
Semen Caryae Cathayensis seedling-raising technique has bigger contribution, but is all to need first to cultivate the rootstock seedling that grows directly from seeds, and is affected with yield by seed quality
Relatively big, cost is high；Need to through seed-soaking, husky hide the multiple working procedures such as lamination, accelerating germination, transplanting, stock nursery stage from winter then to
There is the shortcomings such as cycle length, risk is big, labor intensity is big August in Second Year even to the 3rd year spring.Therefore, in the urgent need to one
Plant the Technique on Cuttage Propagation of pecan tree, the large batch of rootstock seedling of quick obtaining, thus reduce pecan tree seedling cost,
Shorten seedling raise period, and ensure the merit of stock.
" the hard branch of apocarya that Wang Zhengjia et al. invents mainly is had about the research of pecan tree Technique on Cuttage Propagation
Cottage method " (application number: 201310022277.X) and " a kind of promotion apocarya of Liu Yongzhi, Li Yongrong et al. invention
The method that branch adventitious root occurs " (application number: 201310174751.0), " a kind of apocarya hardwood cutting and seedling raising method "
(application number: 201310247748.7), although the former rooting rate reaches 80%, but every year can only cuttage in the last ten-days period in March, the time is shorter,
Cuttage batch is few, the latter two be then winter to spring all can cuttage, but use heating wire to heat, not only energy consumption is too big, with
Shi Yin is by ground hot line embedment wet substrate, and when joint is many, wiring is extremely not easy, and easily open circuit, 1-2 just needs to change
Heating wire, and troublesome poeration and instability.Therefore, for obtaining substantial amounts of cutting seedling, it is necessary to extending cutting time, increasing
On the basis of cuttage lot number, improve cutting plantation, simultaneously the most also it is however emphasized that low-carbon energy-saving, raising stability and guarantee safety.
Utilize solar water heater to heat, just ensure that some requirement above.
Summary of the invention
It is an object of the invention to provide a kind of method improving pecan tree rooting percent, U.S. that the present invention provides
State's Semen Caryae Cathayensis cuttage root-taking method has the advantages such as the time is short, rooting rate is high, cuttage batch is many.
In order to realize the purpose of the present invention, spy by the following technical solutions:
A kind of method improving pecan tree rooting percent, comprises the following steps:
With ultrasonic cell disruption instrument to inserting while using root-inducing powder treatment fluid to soak the cutting sheared on elite stand
Fringe carries out supersound process, by cutting cuttage to Medium Culture after terminating；The power of described ultrasonic cell disruption instrument is 200-300w；
The temperature of described substrate is 20-28 DEG C, water content of substrate 20%-40%；Preferably, described supersound process includes: the most ultrasonic
5-7s, the interval 5-7s of adjacent twice supersound process, the total time of supersound process is 2-4min.It is furthermore preferred that described ultrasonic place
Reason includes: each ultrasonic 5-6s, the interval 5-6s of adjacent twice supersound process, and the total time of supersound process is 3-4min.
The cuttage breeding method of the pecan tree cutting that the present invention provides, has specific temperature controlling substrate and contains
Under conditions of the water yield, under using root-inducing powder functions of hormones, it is aided with specific ultrasonic processing method, it is possible to greatly simultaneously
Stimulate cell division, not only significantly reduce the time taken root, the most also considerably improve rooting rate, moreover it is possible to improve it and support
The ability of imperial natural disaster and promotion growth, have the features such as rooting rate practicality high, simple to operate, low cost, seedling raise period
Short, it is adaptable to extensive cottage propagation.
It addition, for concrete ultrasound wave way choice, each ultrasonic time is long, each sonication times interval
The shortest, stimulate while adding root-inducing powder hormone, easily cause the destruction to cell；Ultrasonic time is too short every time, the most ultrasonic
The time interval of ripple is the longest, and ultrasound wave does not has a fissional effect of ultrasonic stimulation together with root-inducing powder hormone, and the present invention
Ultrasonic frequency, sonication times and the ultrasound wave interval selected, can stimulate the cell division of cutting to greatest extent, be conducive to
Root-inducing powder hormone enters cell interior, promotes the cambium layer in branch and bundle sheath tissue, forms root initial body, thus sends out
Educate and grow adventitious root, and form root system, improve rooting rate.
Preferably, before by cutting cuttage to intramatrical step, also include following preparation: install outside booth
Solar water heater, is placed in steelframe seedbed in booth, lays substrate, sterilization, then lay grid, finally on steel wire
Cover substrate, firing equipment, water circulating pump and hot water pipe net are connected into a hot water cyclesystem, by water circulating pump plug with
Temperature controller is connected, and the temperature probe of temperature controller is inserted Medium Culture.The present invention, by arranging a hot water cyclesystem, controls base
Temperature in the temperature of matter and adjustment booth so that the root of cutting can be always held at the temperature conditions that optimum is taken root
Under, drastically increase rooting rate, and decrease rootage duration.
Preferably, described firing equipment is solar water heater.When using solar water heater as heating source, will too
Sun can be placed in outside booth by water heater.Solar water appliance is used to have low-carbon energy-saving, improve stability and guarantee that safety etc. is excellent
Point；When weather conditions affect the efficiency of heating surface of solar water heater, the electric heater in solar water heater can be opened, can
At low-carbon energy-saving with on the basis of guaranteeing the advantages such as safety, it is ensured that the stability of heating and persistence.
In order to better control over temperature, humidity and the light intensity in booth, it is preferred that cover from sun can be covered outside booth；Will
Cutting cuttage to after Medium Culture, keep temperature of shed less than 18 DEG C, slotting machine upper air humidity 80%-90%；It is furthermore preferred that protect
Hold temperature of shed at 13-18 DEG C.The present invention is by adjusting temperature in booth, humidity, light intensity so that temperature in booth and wet
Degree defines a specific gradient with temperature and the water content of substrate, and under such gradient, will not germinate in the top of cutting, keep away
Exempt from upper and lower competition moisture and the nutrition of cutting, do not resulted in the loss of moisture and nutrition, moisture can be promoted and support
Shunting is taken root to the bottom of cutting, the appearance of the root restriction stimulating pecan tree cutting that is highly advantageous to and growth, promotes
Educated seedling root is luxuriant, drastically increases the cutting plantation of pecan tree cutting, and shortens its rootage duration, carries
High production efficiency.
Preferably, before using root-inducing powder treatment fluid to soak the cutting sheared on elite stand, first cutting is placed in flowing water
After lower flushing 20-30h, then with 40-60%wt acetone soak 1.5-3h, the soaking depth of cutting is 3-5cm.Wherein, 40-60%
Wt acetone is the mixture of acetone and water.
Preferably, described substrate includes turfy soil, perlite and Vermiculitum, and its volume ratio is 4-5:4-5:1.Inventor passes through
A large amount of orthogonal tests, constantly change composition and the content of substrate, finally determine with turfy soil, perlite and Vermiculitum in specific proportions
The substrate of composition, is conducive to shortening the rootage duration of pecan tree cutting, promotes the breeding of its adventitious root.
Preferably, described root-inducing powder treatment fluid is NAA or IBA or ABT or the mixed solution of NAA, IBA and ABT, adopts
The time soaked with root-inducing powder treatment fluid is 2-7h, and in described mixed solution, NAA, IBA are 5:2-10:2-with the mass ratio of ABT
25；The concentration of described root-inducing powder treatment fluid is 400-800mg.L-1.The present invention uses above-mentioned specific root-inducing powder treatment fluid with super
Sonicated method coordinates, and the two has the effect of cooperative compensating, it is possible to strengthen the schizogamy stimulating cutting root cell,
After cuttage 20 days start to take root, and when 40 days, rooting rate reaches more than 62%.
Preferably, the concentration of described root-inducing powder treatment fluid is 400-600mg.L-1, described root-inducing powder treatment fluid be NAA or
NAA, IBA and the mixed solution of ABT, in described mixed solution, NAA, IBA are 5:2-3:2-3 with the mass ratio of ABT, and employing is taken root
The time that powder treatment fluid soaks is 4-7h, and after cuttage 20 days start to take root, and when 40 days, rooting rate reaches more than 81%.
Most preferably, the concentration of described root-inducing powder treatment fluid is 450-550mg.L-1, described root-inducing powder treatment fluid is NAA,
The time using root-inducing powder treatment fluid to soak is 5-6h, and after cuttage 20 days start to take root, and when 40 days, rooting rate reaches more than 86%.
Preferably, described cuttage is directly to insert in mixed-matrix by cutting, and seeding row spacing is 3cm × 5cm, and the cuttage degree of depth is
4-5cm, after then the substrate around cutting being compacted, waters permeable.
Preferably, periodically carrying out disinfection slotting machine, described sterilization is: rotation uses thiophanate methyl or carbendazim wettable
Substrate is carried out disinfection sterilization by property powder.By volume, the consumption of thiophanate methyl is 750-850 times of liquid of thiophanate methyl, many
550-650 times of liquid of the carbendazol wettable powder that consumption is 50%wt of bacterium spirit wettable powder.
Compared with prior art, the invention have the benefit that
(1) cuttage breeding method of pecan tree stock that the present invention provides, have practicality simple to operate, low cost,
Rooting rate high, seedling raise period is short, it is adaptable to extensive cottage propagation；
(2) present invention utilizes Scissors used specially for grafting anvil abandoned stock branch to be material, by skills such as temperature control and hormone immersions
Art link has carried out expanding propagation to nursery stock, turns waste into wealth, and can obtain substantial amounts of high-quality rootstock seedling every year, production has higher application
Promotional value；
(3) present invention is by controlling substrate temperature higher than air themperature in hot house, extends cuttage season, simultaneously
Ensure that cutting germinates after taking root, beneficially the growth of root system, promote educated seedling root luxuriant.
Detailed description of the invention
Embodiment 1
(1) slotting machine prepares
First ground is flattened in booth, build wide 0.8-1m, the steelframe seedbed of high 0.4-0.8m thereon, then at Seedling
Surround cuttage pond with plastic plate high for 30cm or plank on bed, then at the gauze of cuttage pond two-layer 20 mesh, gauze is spread one
The mixed-matrix that layer 4-5cm is thick；Mixed-matrix includes turfy soil, perlite and Vermiculitum, and the volume ratio of this three is 4:5:1.?
Laying DN20 hot water pipe net on mixed-matrix, tube pitch is about 10-15cm, a connection water circulating pump water outlet of hot-water line
Mouthful, the water inlet of water circulating pump is then connected to solar water heater outlet, and the other end of solar water heating pipe then connects the sun
Energy water feeding of heater mouth so that it is become path, then spreads the thick mixed-matrix of 15cm on hot water pipe net, many with 50%wt
Substrate is carried out disinfection sterilization by 650 times of liquid of bacterium spirit wettable powder, and flattens stand-by, by water circulating pump plug with temperature controller phase
Even, being inserted in plugging soil by the temperature probe of temperature controller, switch on power, control slotting machine temperature is at 20 DEG C, and sets solar water
Temperature is more than 50 DEG C, it is ensured that in case of cloudy, rainy day cannot utilize solar energy time, can automatically be electrically heated, it is ensured that slotting machine heat
Transmission.
(2) cutting collection
The raw branch in healthy, full for clip 10cm from scion-seed tree 1 year, upper cut straight snips, lower cut is tiltedly cut away from bud about
0.2cm。
(3) cutting processes
After the cutting sheared is placed under flowing water flushing 24h, then by 50%wt acetone soak 2h, soaking depth 3-4cm；So
It is 450mg.L by concentration afterwards-1NAA root-inducing powder treatment fluid soak cutting 6h, soaking depth 3-4cm.Root-inducing powder process same
Time, with supersonic generator, cutting being carried out supersound process, ultrasonic power is 200w, each ultrasonic 7s, adjacent twice ultrasonic place
The interval 7s of reason, the total time of supersound process is 2min.
(4) cottage method
Cuttage is directly to insert in mixed-matrix by cutting, and seeding row spacing is 2-4cm × 4-5cm, and the cuttage degree of depth is 4-5cm,
Then the substrate around cutting is compacted, and irrigates water immediately.Each process cuttage 100 strain, 3 repetitions.
(5) manage after inserting
Covering one layer of shading rate outside booth is the sunshade net of 70%-90%, and according to humiture situation in booth to canopy
Inside carry out nebulizing spray, keep temperature of shed at 13 DEG C, slotting machine upper air humidity 80%, prevent cutting dehydration from drying up；Keep
Slotting machine substrate temperature 20 DEG C, water content of substrate 20%；Every 7 days carbendazol wettable powders 600 times to slotting machine 50%wt
Liquid carries out disinfection, and within after inserting 7-10 days, produces callus, and 20 days start to take root, and after cuttage 40 days, rooting rate reaches 86.33%, averagely
Indefinite radical 3.2, the longest indefinite root length 6cm.
Embodiment 2
The present embodiment uses the method for embodiment 1, and the difference of embodiment 1 is: the temperature controlling substrate is 25
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L-1, use root-inducing powder treatment fluid to soak cutting 5.5h.
Described supersound process includes: each ultrasonic 6s, the interval 6s of adjacent twice supersound process, and the total time of supersound process is 3min.
Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches 91.67%.Average indefinite radical 3.4
Root, the longest indefinite root length 15.5cm.
Embodiment 3
The present embodiment uses the method for embodiment 1, and the difference of embodiment 1 is: the temperature controlling substrate is 28
DEG C, water content of substrate 40%.The concentration of root-inducing powder treatment fluid is 550mg.L-1, use root-inducing powder treatment fluid to soak cutting 5h.Institute
State supersound process to include: ultrasonic power is 300w, each ultrasonic 5s, the interval 5s of adjacent twice supersound process, supersound process
Total time is 4min.Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches 88.33%.Flat
The most indefinite radical 3.3, the longest indefinite root length 5.3cm.
Embodiment 4
(1) slotting machine prepares
First ground is flattened in booth, build wide 0.8-1m, the steelframe seedbed of high 0.4-0.8m thereon, then at Seedling
Surround cuttage pond with plastic plate high for 30cm or plank on bed, then at the gauze of cuttage pond two-layer 20 mesh, gauze is spread one
The mixed-matrix that layer 4-5cm is thick；Mixed-matrix includes turfy soil, perlite and Vermiculitum, and the volume ratio of this three is 5:4:1.?
Laying DN20 hot water pipe net on mixed-matrix, tube pitch is about 12cm, a connection water circulating pump outlet of hot-water line, follows
The water inlet of ring water pump is then connected to solar water heater outlet, and the other end of solar water heating pipe then connects solar water
Device water inlet so that it is become path, then spreads the thick mixed-matrix of 10-15cm, with many bacterium of 50%wt on hot water pipe net
Substrate is carried out disinfection sterilization by spirit 600 times of liquid of wettable powder, and flattens stand-by, by water circulating pump plug with temperature controller phase
Even, being inserted in plugging soil by the temperature probe of temperature controller, switch on power, control slotting machine temperature is between 25 DEG C, and sets solar heat
Coolant-temperature gage is more than 50 DEG C, it is ensured that in case of cloudy, rainy day cannot utilize solar energy time, can automatically be electrically heated, it is ensured that slotting machine heat
Amount transmission.
(2) cutting collection
The raw branch in healthy, full for clip 10cm from scion-seed tree 1 year, upper cut straight snips, lower cut is tiltedly cut away from bud about
0.2cm。
(3) cutting processes
After the cutting sheared is placed under flowing water flushing 30h, then by 60%wt acetone soak 1.5h, soaking depth 3-5cm；
Then it is 500mg.L by concentration-1The mixed solution of NAA, IBA and ABT soak cutting 7h, wherein, the matter of NAA, IBA and ABT
Amount ratio is 5:3:2.While root-inducing powder processes, with supersonic generator, cutting being carried out supersound process, ultrasonic power is
200w, each ultrasonic 7s, the interval 7s of adjacent twice supersound process, the total time of supersound process is 4min.
(4) cottage method
Cuttage is directly to insert in mixed-matrix by cutting, and seeding row spacing is 2-4cm × 4-6cm, and the cuttage degree of depth is 4-5cm,
Then the substrate around cutting is compacted, and irrigates water immediately.Each process cuttage 100 strain, 3 repetitions.
(5) manage after inserting
Covering one layer of shading rate outside booth is the sunshade net of 70%-90%, and according to humiture situation in booth to canopy
Inside carry out nebulizing spray, keep temperature of shed at 18 DEG C, slotting machine upper air humidity 90%, prevent cutting dehydration from drying up；Keep
Slotting machine substrate temperature 25 DEG C, water content of substrate 40%；Every 7 days, 800 times of liquid of slotting machine sterling thiophanate methyl are carried out disinfection,
Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches 81.67%.Average indefinite radical 2.8
Root, the longest indefinite root length 10.2cm.
Embodiment 5
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 400mg.L-1, wherein, NAA, IBA are 5 with the mass ratio of ABT:
2:3, uses root-inducing powder treatment fluid to soak cutting 7h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s, phase
The interval 7s of adjacent twice supersound process, the total time of supersound process is 4min.Within after inserting 7-10 days, producing callus, 20 days start
Taking root, after cuttage 40 days, rooting rate reaches 82.66%.Average indefinite radical 2.5, the longest indefinite root length 8.2cm.
Embodiment 6
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 800mg.L-1NAA liquid, use root-inducing powder treatment fluid soak insert
Fringe 2h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice supersound process is ultrasonic
The total time processed is 4min.Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches
64.33%.Average indefinite radical 2, the longest indefinite root length 4.8cm.
Embodiment 7
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L-1IBA liquid, use root-inducing powder treatment fluid soak insert
Fringe 6h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice supersound process is ultrasonic
The total time processed is 4min.Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches
62.33%.Average indefinite radical 2, the longest indefinite root length 6.9cm.
Embodiment 8
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L-1ABT liquid, use root-inducing powder treatment fluid soak insert
Fringe 6h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s, and the interval 7s of adjacent twice supersound process is ultrasonic
The total time processed is 4min.Within after inserting 7-10 days, producing callus, 20 days start to take root, and after cuttage 40 days, rooting rate reaches
68.33%.Average indefinite radical 2.2, the longest indefinite root length 10.1cm.
Embodiment 9
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L-1, wherein, NAA, IBA are 5 with the mass ratio of ABT:
10:25, uses root-inducing powder treatment fluid to soak cutting 6h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s,
The interval 7s of adjacent twice supersound process, the total time of supersound process is 4min.Within after inserting 7-10 days, produce callus, within 20 days, open
Beginning takes root, and after cuttage 40 days, rooting rate reaches 68.66%.Average indefinite radical 2, the longest indefinite root length 6.9cm.
Embodiment 10
The present embodiment uses the method for embodiment 4, and the difference of embodiment 4 is: the temperature controlling substrate is 28
DEG C, water content of substrate 30%.The concentration of root-inducing powder treatment fluid is 500mg.L-1, wherein, NAA, IBA are 5 with the mass ratio of ABT:
2:2, uses root-inducing powder treatment fluid to soak cutting 7h.Described supersound process includes: ultrasonic power is 300w, each ultrasonic 7s, phase
The interval 7s of adjacent twice supersound process, the total time of supersound process is 4min.Within after inserting 7-10 days, producing callus, 20 days start
Taking root, after cuttage 40 days, rooting rate reaches 81.67%.Average indefinite radical 2.3, the longest indefinite root length 7.3cm.
Test example 1
This test example compares the rooting method of the embodiment of the present invention and the rooting method of corresponding comparable group to U.S. mountain
The impact of Semen Juglandis rooting percent.
Comparable group 1-10 is respectively adopted the rooting method numbering corresponding embodiment 1-10 with it, and difference is ratio
Sample in relatively group does not the most use supersound process.
Clear water matched group uses the rooting method of embodiment 1, and difference is: do not use root-inducing powder treatment fluid to soak,
And using clear water to soak 6h, the rooting rate after using ultrasonic Treatment and not using ultrasonic Treatment is 0.
Table 1 hormon and the supersound process impact on pecan tree cutting plantation
| The rooting rate of embodiment | The rooting rate of comparative example | |
| Numbering 1 | 86.33% | 57.0% |
| Numbering 2 | 91.67% | 58.33% |
| Numbering 3 | 88.33% | 57.66% |
| Numbering 4 | 81.67% | 52.67% |
| Numbering 5 | 82.66% | 53% |
| Numbering 6 | 64.33% | 47.67% |
| Numbering 7 | 62.33% | 31.11% |
| Numbering 8 | 68.33% | 38.67% |
| Numbering 9 | 68.66% | 46% |
| Numbering 10 | 81.67% | 52.67% |
As can be seen from Table 1: sonicated rear pecan tree rooting percent can improve 16.66%-33.34%.
The rooting percent of different root-growing agent combined treatment changes along with hormone concentration and the difference of soak time.When using concentration it is
450-550mg.L-1NAA root-inducing powder treatment fluid soak 5-6 hour, and combine ultrasonic Treatment, rooting rate reaches more than 86%.When
Employing concentration is 400-600mg.L-1NAA or NAA, IBA with ABT mixed solution as root-inducing powder treatment fluid soak 4-7 little
Time, in conjunction with ultrasonic Treatment, rooting rate reaches more than 81%.It is 400-800mg.L when using concentration-1NAA or IBA or ABT,
Or the mixed solution of NAA, IBA and ABT soaks 2-7 hour as root-inducing powder treatment fluid, in conjunction with ultrasonic Treatment, rooting rate reaches
More than 62%.And when soaking 2h with clear water, in spite of through supersound process, cutting all cannot be taken root in hotbed.
Claims (8)
1. the method improving pecan tree rooting percent, it is characterised in that comprise the following steps:
The cutting sheared on elite stand use root-inducing powder treatment fluid enter cutting with ultrasonic cell disruption instrument while soaking
Row supersound process, by cutting cuttage to Medium Culture after terminating；The power of described ultrasonic cell disruption instrument is 200-300w；Described
Supersound process includes: each ultrasonic 5-7s, the interval 5-7s of adjacent twice supersound process, and the total time of supersound process is 2-
4min；
Described root-inducing powder treatment fluid is NAA, or the mixed solution of NAA, IBA and ABT, NAA, IBA and ABT in described mixed solution
Mass ratio be 5:2-3:2-3, use root-inducing powder treatment fluid soak time be 4-7h, the concentration of described root-inducing powder treatment fluid is
400-600mg.L-1；
The temperature of described substrate is 20-28 DEG C, water content of substrate 20%-40%；After cutting cuttage to Medium Culture, in keeping canopy
Temperature at 13-18 DEG C, slotting machine upper air humidity 80%-90%.
Method the most according to claim 1, it is characterised in that before by cutting cuttage to intramatrical step, also wrap
Include following preparation: solar water heater is installed outside booth, steelframe seedbed is placed in booth, steel wire is laid base
Matter, sterilization, then lay grid, finally cover substrate, firing equipment, water circulating pump and hot water pipe net are connected into a heat
Water circulation system, is connected water circulating pump plug with temperature controller, and the temperature probe of temperature controller is inserted Medium Culture.
Method the most according to claim 2, it is characterised in that described firing equipment is solar water heater.
Method the most according to claim 1, it is characterised in that the cutting sheared on elite stand is used root-inducing powder treatment fluid
Before immersion, after first cutting is placed under flowing water flushing 20-30h, then with 40-60%wt acetone soak 1.5-3h, the leaching of cutting
The bubble degree of depth is 3-5cm.
Method the most according to claim 1, it is characterised in that described substrate includes turfy soil, perlite and Vermiculitum, its body
Long-pending ratio is 4-5:4-5:1.
Method the most according to claim 1, it is characterised in that the concentration of described root-inducing powder treatment fluid is 450-550mg.L-1, described root-inducing powder treatment fluid is NAA, and the time using root-inducing powder treatment fluid to soak is 5-6h.
Method the most according to claim 1, it is characterised in that described cuttage is directly to insert in mixed-matrix by cutting,
Seeding row spacing is 2-4cm × 4-6cm, and the cuttage degree of depth is 4-5cm, after then the substrate around cutting being compacted, waters permeable.
Method the most according to claim 1, it is characterised in that periodically carry out disinfection slotting machine, described sterilization is: rotation
Thiophanate methyl or carbendazol wettable powder is used substrate to be carried out disinfection sterilization.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201410286988.2A CN103999706B (en) | 2014-06-24 | 2014-06-24 | A kind of method improving pecan tree rooting percent |
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| CN201410286988.2A CN103999706B (en) | 2014-06-24 | 2014-06-24 | A kind of method improving pecan tree rooting percent |
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| CN104521672B (en) * | 2014-12-24 | 2017-01-04 | 普定县四海农业发展有限公司 | Walnut planting method |
| CN104823790B (en) * | 2015-04-29 | 2018-03-20 | 河池学院 | A kind of bulla walnut cuttage fast seedling-cultivating method |
| CN104945139B (en) * | 2015-06-26 | 2018-06-05 | 江苏水木农景股份有限公司 | A kind of pecan tree culture substrate and preparation method thereof |
| CN105532294A (en) * | 2015-12-08 | 2016-05-04 | 北京农学院 | Cutting breeding method for improving cutting rooting percentage of Liriodendron Chinese |
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| CN106718536A (en) * | 2016-12-01 | 2017-05-31 | 南京绿宙薄壳山核桃科技有限公司 | It is a kind of to heat the method that bed carries out apocarya cuttage using fermentation |
| CN106577237A (en) * | 2016-12-23 | 2017-04-26 | 江苏农林职业技术学院 | Method of cutting in winter for saniodendron subaequalum |
| CN107223506B (en) * | 2017-05-25 | 2018-07-13 | 马鞍山市绿营林业有限公司 | A kind of method for culturing seedlings of thin skin hickory nut |
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| CN107624394A (en) * | 2017-08-24 | 2018-01-26 | 浦江县合洪园艺研发有限公司 | A kind of method for inducing hickory nut adventitious root to occur |
| CN108781805A (en) * | 2018-04-11 | 2018-11-13 | 霍山县庆中农林开发有限公司 | A kind of cuttage processing method improving safflower oil tea cuttage radication capability |
| CN110637622A (en) * | 2019-11-05 | 2020-01-03 | 中国林业科学研究院亚热带林业实验中心 | Method for asexual cuttage propagation of vernicia fordii |
| CN111345163A (en) * | 2020-03-18 | 2020-06-30 | 吉林省林业科学研究院 | Rapid rooting method for tilia amurensis cutting slips |
| CN113303114A (en) * | 2021-05-18 | 2021-08-27 | 郑州市农林科学研究所 | Method for reducing total tannin content of cyclobalanopsis glauca cutting slips |
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| CN101743829A (en) * | 2009-12-08 | 2010-06-23 | 山西省农业生物技术研究中心 | Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment |
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| JP4868812B2 (en) * | 2005-09-30 | 2012-02-01 | 日本製紙株式会社 | Cutting method of cuttings |
| RU2332838C1 (en) * | 2007-01-31 | 2008-09-10 | Государственное общеобразовательное учреждение высшего профессионального образования "Алтайский государственный технический университет им. И.И. Ползунова" (АлтГТУ) | Method of reproduction of grafts of grapes |
| CN101578957A (en) * | 2009-06-02 | 2009-11-18 | 侯金波 | Ultrasound quick-breeding system |
| CN102138485A (en) * | 2011-03-22 | 2011-08-03 | 中国科学院昆明植物研究所 | Full-exposure cuttage and propagation method of camellia reticulata |
| CN102657014A (en) * | 2012-05-20 | 2012-09-12 | 何寒 | Method for raising golden camellia seedlings by cutting |
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| CN101743829A (en) * | 2009-12-08 | 2010-06-23 | 山西省农业生物技术研究中心 | Method for improving cuttage rooting and survival rate of shrub woody plant with ultrasonic treatment |
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