CN102577877B - Method for quick propagation of cloned seedlings of Oreorchis patens - Google Patents
Method for quick propagation of cloned seedlings of Oreorchis patens Download PDFInfo
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- CN102577877B CN102577877B CN 201210086801 CN201210086801A CN102577877B CN 102577877 B CN102577877 B CN 102577877B CN 201210086801 CN201210086801 CN 201210086801 CN 201210086801 A CN201210086801 A CN 201210086801A CN 102577877 B CN102577877 B CN 102577877B
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Abstract
The invention relates to a method for quick propagation of cloned seedlings of Oreorchis patens, which includes: setting up a plastic tunnel on a land with no waterlogging; making even beds 1-1.5m wide on the land surface in the tunnel, and serving operation ways 20-30 wide among the beds, wherein surface soil of each bed is loose and in a tile back shape; well mixing locally taken loose soil with decayed farm manure according to a proportion 3:0.5-1.5 to obtain seedbed nutritive soil, and spreading the nutritive soil on each bed according to the thickness 5-8cm; spreading a moist river sand layer 10cm thick on each bed, and using disinfectant to disinfect the river sand; selecting Oreorchis patens pseudobulbs roughly in the same size, each containing three to five bud eyes, as breeding objects in the harvesting time; and after sterilizing the Oreorchis patens pseudobulbs for 2-8 days, placing the pseudobulbs in the moist river sand inside the plastic tunnel for accelerating germination, and taking down buds growing to 1-2cm long from the pseudobulbs. The method has the advantages that seedling raising time is short, disease and insect damage are low, large-scale raising is easy, cost is low, benefit is high, and the method is easy to operate.
Description
Technical field
The present invention relates to the planting technology field, relate in particular to the fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe.
Background technology
Pleionebulbocodioides rolfe (
Oreorchis patens (Lindl.) Lindl.)The soccer fraud stem of another name edible tulip, PSEUDOBULBUS CREMASTRAE APPENDICULATAE, the perennial the orchid family herbaceous plant of mud guest subsystem pleione bulbocodioides is a kind of rare Chinese herbal medicine.Have detumescence, dissipating bind, the moistening lung of reducing phlegm, detoxify, nourish heart, effect such as calm the nerves with all worries set aside.Pleionebulbocodioides rolfe is born in height above sea level such as high mountain cogongrass slope more than 1000 meters more.Past artificial cultivation pleionebulbocodioides rolfe mainly adopts traditional seed growing to obtain seedling and cultivates, because though this seed growing can be obtained a spot of seedling, but because of seed tiny, bad grasp of maturing stage, the seed collection rate is low, it is low to germinate, poor growth, many, the seedling lethality height of damage by disease and insect, seriously restricted the rapid expansion that pleionebulbocodioides rolfe is cultivated scale.
Summary of the invention
The objective of the invention is to overcome above-mentioned shortcoming and provide that a kind of tool seedling raise period is short, damage by disease and insect is few, formed scale easily, cost is low, the fast breeding method of high efficiency, the asexual seedling of easy-operating pleionebulbocodioides rolfe.
The fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe of the present invention comprises the steps:
(1) place of growing seedlings: select the physical features of no ponding to have certain slope or level land to build plastic tunnel, wide 1-1.5 rice railway carriage or compartment face is made with uprooting in ground in the canopy, and the railway carriage or compartment face will flatten, and stays 20-30 centimetre wide operation road between the railway carriage or compartment, and railway carriage or compartment face loosing soil is a watt antiform;
(2) preparation of cover soil: get loose soil and well-rotted farmyard manure on the spot and get cover soil in the ratio mixing of 3:0.5-1.5, then nutrition soil is spread on the railway carriage or compartment face, thickness 5-8 centimetre;
(3) river sand sterilization: to spread moistening thickness be river sand about 10cm to the railway carriage or compartment face in the plastic tunnel of building up, and sterilize with disinfectant;
(4) pleionebulbocodioides rolfe kind ball is selected: select big or small basically identical in picking time, contain 3-5 eye pleionebulbocodioides rolfe soccer fraud stem and do the breeding object;
(5) seedling is cultivated: with plant ash and lime in the ratio mixing that is 7:3, clean the earth that pleionebulbocodioides rolfe soccer fraud stem has earlier, use film wrapped then, cover fully with above-mentioned combination ash, after carrying out 2-8 days sterilization, remove film, insert vernalization in the river sand moistening in the plastic tunnel, treat that tender shoots grows when arriving 1-2cm, takes off tender shoots on the pleionebulbocodioides rolfe and namely gets seedling; The sprouting sprouting that will grow when getting bud is located to separate with the soccer fraud stem, has several buds just to be divided into several.
The fast breeding method of the above-mentioned asexual seedling of a kind of pleionebulbocodioides rolfe, wherein: the disinfectant of river sand sterilization usefulness refers to carry out disinfection after with 500-1000 times of water dilution with fenaminosulf.
The fast breeding method of the above-mentioned asexual seedling of a kind of pleionebulbocodioides rolfe, wherein: river sand is that the thin river sand of 40 purposes is crossed in the korneforos.
The fast breeding method of the above-mentioned asexual seedling of a kind of pleionebulbocodioides rolfe, wherein seedling management: 1. regularly water: look table soil and turn white and then water immediately, require to water less, duty waters, and keeps soil moisture content about 65%; 2. heat and moisture preserving with take off canopy and ventilate: generally keep 10 ℃-20 ℃ of temperature of shed, humidity about 80%; 3. weeding: duty is cut weeds, and keeps the seedbed not have weeds.
The fast breeding method of the above-mentioned asexual seedling of a kind of pleionebulbocodioides rolfe, wherein, the method that transplant the back of growing seedlings is: tender shoots is pressed strain
Cultivate, compress gently with spades, reserve tender shoots, water evenly irrigates, but can not ponding.
The present invention compared with prior art, has tangible beneficial effect, as can be known from the above technical solutions: the present invention breeds the pleionebulbocodioides rolfe seedling with the soccer fraud stem, have that the seedling cost is low, plant strain growth is fast, seedling raise period is short, damage by disease and insect is few, output forms scale, high efficiency greatly, easily, cost is low, output is high, easy-operating.Therefore the present invention is significant to the industrialization development that promotes pleionebulbocodioides rolfe, and is significant for seeking the road of shaking off poverty and setting out on the road to prosperity for high altitude mountainous area rural economic development especially, the present invention simultaneously also be fit to useful soccer fraud stem breed the plant of seedling.
Embodiment
Embodiment 1
The fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe comprises the steps:
(1) place of growing seedlings: select physical features to have the plot of certain slope (to avoid ponding) to build plastic tunnel, wide 1-1.5 rice railway carriage or compartment face is made with uprooting in ground in the canopy, and the railway carriage or compartment face will flatten, and stays 20-30 centimetre wide operation road between the railway carriage or compartment, and railway carriage or compartment face loosing soil is a watt antiform;
(2) preparation of cover soil: get loose soil and preprepared well-rotted farmyard manure on the spot and get cover soil in the ratio mixing of 3:1, then nutrition soil is spread on the railway carriage or compartment face, thickness 5-8 centimetre;
(3) river sand sterilization: the railway carriage or compartment face is spread the river sand that moistening thickness is 10 left and right sides cm (the thin river sand of 40 purposes is crossed in the korneforos) in the plastic tunnel of building up, and sterilizes with disinfectant (fenaminosulf dilutes with 500 times of water);
(4) pleionebulbocodioides rolfe kind ball is selected: select big or small basically identical in picking time, contain 3-5 eye pleionebulbocodioides rolfe soccer fraud stem and do the breeding object;
(5) seedling is cultivated: with plant ash and lime in the ratio mixing that is 7:3, clean the earth that pleionebulbocodioides rolfe soccer fraud stem has earlier, use film wrapped then, cover fully with above-mentioned combination ash, after carrying out 2 days sterilization, remove film, insert vernalization in the river sand moistening in the plastic tunnel, when treating tender shoots length to 1cm, take off tender shoots on the pleionebulbocodioides rolfe standby; The sprouting sprouting that will grow when getting bud is located to separate with the soccer fraud stem, generally has several buds just to be divided into several.
Seedling management: 1. regularly water: look table soil and turn white and then water immediately, require to water less, duty waters, and keeps soil moisture content about 65%; 2. heat and moisture preserving with take off canopy and ventilate: generally keep 10 ℃-20 ℃ of temperature of shed, humidity about 80%; 3. weeding: duty is cut weeds, and keeps the seedbed not have weeds.
Embodiment 2
The fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe comprises the steps:
(1) place of growing seedlings: select physical features that certain slope is arranged, to avoid ponding, plastic tunnel is built in the plot of (if ponding will be noted in the level land), wide 1-1.5 rice railway carriage or compartment face is made with hoe in the field in the canopy, and the railway carriage or compartment face will flatten, and stays 20-30 centimetre wide operation road between the railway carriage or compartment, railway carriage or compartment face loosing soil is a watt antiform;
(2) preparation of cover soil: get loose soil and preprepared well-rotted farmyard manure on the spot and get cover soil in the ratio mixing of 3:0.5, then nutrition soil is spread on the railway carriage or compartment face, thickness 5-8 centimetre;
(3) river sand sterilization: to spread moistening thickness be river sand (the thin river sand of 40 purposes is crossed in the korneforos) about 10cm to the railway carriage or compartment face in the plastic tunnel of building up, and sterilize with disinfectant (fenaminosulf dilutes with 800 times of water);
(4) pleionebulbocodioides rolfe kind ball is selected: select big or small basically identical in picking time, contain 3-5 eye pleionebulbocodioides rolfe soccer fraud stem and do the breeding object;
(5) seedling is cultivated: with plant ash and lime in the ratio mixing that is 7:3, clean the earth that pleionebulbocodioides rolfe soccer fraud stem has earlier, use film wrapped then, cover fully with above-mentioned combination ash, after carrying out 8 days sterilization, remove film, insert vernalization in the river sand moistening in the plastic tunnel, when treating tender shoots length to 2cm, take off tender shoots on the pleionebulbocodioides rolfe standby; The sprouting sprouting that will grow when getting bud is located to separate with the soccer fraud stem, generally has several buds just to be divided into several.
Seedling management: 1. regularly water: look table soil and turn white and then water immediately, require to water less, duty waters, and keeps soil moisture content about 65%; 2. heat and moisture preserving with take off canopy and ventilate: generally keep 10 ℃-20 ℃ of temperature of shed, humidity about 80%; 3. weeding: duty is cut weeds, and keeps the seedbed not have weeds.
Embodiment 3
The fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe comprises the steps:
(1) place of growing seedlings: select physical features that certain slope is arranged, to avoid ponding, plastic tunnel is built in the plot of (if ponding will be noted in the level land), wide 1-1.5 rice railway carriage or compartment face is made with hoe in the field in the canopy, and the railway carriage or compartment face will flatten, and stays 20-30 centimetre wide operation road between the railway carriage or compartment, railway carriage or compartment face loosing soil is a watt antiform;
(2) preparation of cover soil: get loose soil and preprepared well-rotted farmyard manure on the spot and get cover soil in the ratio mixing of 3:1.5, then nutrition soil is spread on the railway carriage or compartment face, thickness 5-8 centimetre;
(3) river sand sterilization: to spread moistening thickness be river sand (the thin river sand of 40 purposes is crossed in the korneforos) about 10cm to the railway carriage or compartment face in the plastic tunnel of building up, and sterilize with disinfectant (fenaminosulf dilutes with 1000 times of water);
(4) pleionebulbocodioides rolfe kind ball is selected: select big or small basically identical in picking time, contain 3-5 eye pleionebulbocodioides rolfe soccer fraud stem and do the breeding object;
(5) seedling is cultivated: with plant ash and lime in the ratio mixing that is 7:3, clean the earth that pleionebulbocodioides rolfe soccer fraud stem has earlier, use film wrapped then, cover fully with above-mentioned combination ash, after carrying out 2-8 days sterilization, remove film, inserted in the river sand moistening in the plastic tunnel vernalization 15-30 days, when treating tender shoots length to 1-2cm, take off tender shoots on the pleionebulbocodioides rolfe standby; The sprouting sprouting that will grow when getting bud is located to separate with the soccer fraud stem, generally has several buds just to be divided into several.
Seedling management: 1. regularly water: look table soil and turn white and then water immediately, require to water less, duty waters, and keeps soil moisture content about 65%; 2. heat and moisture preserving with take off canopy and ventilate: generally keep 10 ℃-20 ℃ of temperature of shed, humidity about 80%; 3. weeding: duty is cut weeds, and keeps the seedbed not have weeds.
(6) transplant after growing seedlings: with tender shoots by strain
Cultivate, compress gently with spades, reserve tender shoots, water evenly irrigates, but can not ponding.
Claims (4)
1. the fast breeding method of the asexual seedling of pleionebulbocodioides rolfe comprises the steps:
(1) place of growing seedlings: select the physical features of no ponding to have certain slope or level land to build plastic tunnel, wide 1-1.5 rice railway carriage or compartment face is made on the interior ground of canopy, and the railway carriage or compartment face will flatten, and stays 20-30 centimetre wide operation road between the railway carriage or compartment, and railway carriage or compartment face loosing soil is a watt antiform;
(2) preparation of cover soil: get loose soil and well-rotted farmyard manure on the spot and get cover soil in the ratio mixing of 3:0.5-1.5, then nutrition soil is spread on the railway carriage or compartment face, thickness 5-8 centimetre;
(3) river sand sterilization: spread the river sand that moistening thickness is 10cm at the railway carriage or compartment face, and sterilize with disinfectant;
(4) pleionebulbocodioides rolfe kind ball is selected: select big or small basically identical in picking time, contain 3-5 eye pleionebulbocodioides rolfe soccer fraud stem and do the breeding object;
(5) seedling is cultivated: with plant ash and the lime ratio mixing in 7:3, clean the earth that pleionebulbocodioides rolfe soccer fraud stem has earlier, use film wrapped then, cover fully with above-mentioned combination ash, after carrying out 2-8 days sterilization, remove film, insert vernalization in the river sand moistening in the plastic tunnel, treat that tender shoots grows when arriving 1-2cm, takes off tender shoots on the pleionebulbocodioides rolfe and namely gets seedling;
Seedling management: 1. regularly water: look table soil and turn white and then water immediately, require to water less, duty waters, and keeps soil moisture content about 65%; 2. heat and moisture preserving with take off canopy and ventilate: generally keep 10 ℃-20 ℃ of temperature of shed, humidity about 80%; 3. weeding: duty is cut weeds, and keeps the seedbed not have weeds;
(6) transplant after growing seedlings: with tender shoots by strain
Cultivate, compress gently with spades, reserve tender shoots, water evenly irrigates, but can not ponding.
2. the fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe as claimed in claim 1, wherein: will grow when getting bud and sprout the sprouting place and separate with the soccer fraud stem, and have several buds just to be divided into several.
3. the fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe as claimed in claim 1 or 2, wherein: the disinfectant of river sand sterilization usefulness refers to carry out disinfection after with 500-1000 times of water dilution with fenaminosulf.
4. the fast breeding method of the asexual seedling of a kind of pleionebulbocodioides rolfe as claimed in claim 3, wherein river sand is that the thin river sand of 40 purposes is crossed in the korneforos.
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| CN102577877B true CN102577877B (en) | 2013-08-21 |
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| CN103749032B (en) * | 2014-01-07 | 2015-07-08 | 陕西师范大学 | Bletilla striata germination accelerating method |
| CN110856470A (en) * | 2018-08-23 | 2020-03-03 | 贵州省台江县西南苗药原生态研究开发有限公司 | Rapid breeding method of bletilla striata asexual seedlings |
| CN114902950A (en) * | 2021-02-06 | 2022-08-16 | 李有荣 | Sexual propagation breeding method for wild ice hockey in northeast region |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147446A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Iphigenia indica kunth et benth seed grow seedling method |
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Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101147446A (en) * | 2007-10-18 | 2008-03-26 | 云南省农业科学院高山经济植物研究所 | Iphigenia indica kunth et benth seed grow seedling method |
Non-Patent Citations (4)
| Title |
|---|
| 丽江山慈菇栽培技术研究初报;袁理春;《中国中药杂志》;20030630;全文 * |
| 丽江山慈菇种子繁殖技术初报;甄莉等;《中国农学通报》;20060228;全文 * |
| 甄莉等.丽江山慈菇种子繁殖技术初报.《中国农学通报》.2006,全文. |
| 袁理春.丽江山慈菇栽培技术研究初报.《中国中药杂志》.2003,全文. |
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