CN106718721A - A kind of domesticating cultivation method of bletilla tissue culture seedlings - Google Patents
A kind of domesticating cultivation method of bletilla tissue culture seedlings Download PDFInfo
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- CN106718721A CN106718721A CN201611045002.8A CN201611045002A CN106718721A CN 106718721 A CN106718721 A CN 106718721A CN 201611045002 A CN201611045002 A CN 201611045002A CN 106718721 A CN106718721 A CN 106718721A
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- bletilla
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- 241001313855 Bletilla Species 0.000 title claims abstract description 29
- 238000012364 cultivation method Methods 0.000 title claims abstract description 14
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 46
- 239000011159 matrix material Substances 0.000 claims abstract description 29
- 210000003608 fece Anatomy 0.000 claims abstract description 26
- 239000010871 livestock manure Substances 0.000 claims abstract description 26
- 239000000203 mixture Substances 0.000 claims abstract description 21
- 239000002689 soil Substances 0.000 claims abstract description 20
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims abstract description 19
- 235000011613 Pinus brutia Nutrition 0.000 claims abstract description 19
- 241000018646 Pinus brutia Species 0.000 claims abstract description 19
- 241001494479 Pecora Species 0.000 claims abstract description 18
- 241000287828 Gallus gallus Species 0.000 claims abstract description 9
- 244000068988 Glycine max Species 0.000 claims abstract description 8
- 235000010469 Glycine max Nutrition 0.000 claims abstract description 8
- 238000005286 illumination Methods 0.000 claims abstract description 6
- 241001313857 Bletilla striata Species 0.000 claims description 15
- 238000004659 sterilization and disinfection Methods 0.000 claims description 13
- 239000007921 spray Substances 0.000 claims description 12
- 238000003756 stirring Methods 0.000 claims description 10
- 230000001954 sterilising effect Effects 0.000 claims description 9
- TWFZGCMQGLPBSX-UHFFFAOYSA-N Carbendazim Natural products C1=CC=C2NC(NC(=O)OC)=NC2=C1 TWFZGCMQGLPBSX-UHFFFAOYSA-N 0.000 claims description 5
- 239000005842 Thiophanate-methyl Substances 0.000 claims description 5
- 239000006013 carbendazim Substances 0.000 claims description 5
- JNPZQRQPIHJYNM-UHFFFAOYSA-N carbendazim Chemical compound C1=C[CH]C2=NC(NC(=O)OC)=NC2=C1 JNPZQRQPIHJYNM-UHFFFAOYSA-N 0.000 claims description 5
- 238000007789 sealing Methods 0.000 claims description 5
- 239000010802 sludge Substances 0.000 claims description 5
- QGHREAKMXXNCOA-UHFFFAOYSA-N thiophanate-methyl Chemical group COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC QGHREAKMXXNCOA-UHFFFAOYSA-N 0.000 claims description 5
- 239000000645 desinfectant Substances 0.000 claims description 4
- 230000000844 anti-bacterial effect Effects 0.000 claims description 3
- 239000003795 chemical substances by application Substances 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 239000007788 liquid Substances 0.000 claims description 2
- RTKIYNMVFMVABJ-UHFFFAOYSA-L thimerosal Chemical compound [Na+].CC[Hg]SC1=CC=CC=C1C([O-])=O RTKIYNMVFMVABJ-UHFFFAOYSA-L 0.000 claims description 2
- 229940033663 thimerosal Drugs 0.000 claims description 2
- 238000012797 qualification Methods 0.000 abstract description 6
- 238000000034 method Methods 0.000 abstract description 2
- 239000008159 sesame oil Substances 0.000 abstract 2
- 235000011803 sesame oil Nutrition 0.000 abstract 2
- 239000002893 slag Substances 0.000 abstract 2
- 206010027146 Melanoderma Diseases 0.000 description 4
- 239000003337 fertilizer Substances 0.000 description 4
- 239000002435 venom Substances 0.000 description 3
- 210000001048 venom Anatomy 0.000 description 3
- 231100000611 venom Toxicity 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 238000003973 irrigation Methods 0.000 description 2
- 230000002262 irrigation Effects 0.000 description 2
- STECJAGHUSJQJN-GAUPFVANSA-N Hyoscine Natural products C1([C@H](CO)C(=O)OC2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-GAUPFVANSA-N 0.000 description 1
- 208000005168 Intussusception Diseases 0.000 description 1
- 229920000057 Mannan Polymers 0.000 description 1
- STECJAGHUSJQJN-UHFFFAOYSA-N N-Methyl-scopolamin Natural products C1C(C2C3O2)N(C)C3CC1OC(=O)C(CO)C1=CC=CC=C1 STECJAGHUSJQJN-UHFFFAOYSA-N 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 239000000022 bacteriostatic agent Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 238000004140 cleaning Methods 0.000 description 1
- 239000000084 colloidal system Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 230000023597 hemostasis Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 210000004072 lung Anatomy 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003020 moisturizing effect Effects 0.000 description 1
- 230000001114 myogenic effect Effects 0.000 description 1
- 239000003415 peat Substances 0.000 description 1
- 239000002304 perfume Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- STECJAGHUSJQJN-FWXGHANASA-N scopolamine Chemical compound C1([C@@H](CO)C(=O)O[C@H]2C[C@@H]3N([C@H](C2)[C@@H]2[C@H]3O2)C)=CC=CC=C1 STECJAGHUSJQJN-FWXGHANASA-N 0.000 description 1
- 229960002646 scopolamine Drugs 0.000 description 1
- 235000013599 spices Nutrition 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 239000002562 thickening agent Substances 0.000 description 1
- 230000007704 transition Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/23—Wood, e.g. wood chips or sawdust
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Cultivation Of Plants (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a kind of domesticating cultivation method of bletilla tissue culture seedlings, comprise the following steps：A, matrix prepare, and matrix includes the mixture and sawdust, chicken manure, soya-bean cake, the mixture of sesame oil slag of pine nut shell, fertile soil and sheep manure；B, cultivating pool is dug in greenhouse, C, be layed in cultivating pool the mixture of the mixture and sawdust of pine nut shell, fertile soil and sheep manure, chicken manure, soya-bean cake, sesame oil slag successively；D, selection bletilla tissue culture seedlings, clean, sterilize, dry；E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX to 3000LX, the daily 10h to 13h of light application time；Periodically water；Crop field can be transplanted after 3 months.Bletilla tissue culture seedlings are tamed using this method, ensure that tissue-cultured seedling grows well, met field-transplanting desired ratio and greatly increase, improve the qualification rate of tissue-cultured seedling.
Description
Technical field
The present invention relates to bletilla striata planting technology field, and in particular to a kind of domesticating cultivation method of bletilla tissue culture seedlings.
Background technology
The bletilla striata is one kind of orchid family bletilla striata category.Perennial herb, high 20~50 centimetres, 4~5 pieces of leaf, the mutual intussusception of base portion
Into bulbous, scape is extracted in center out.Raceme has several flowers；Flower purple or pale red, about 5 centimetres of diameter, by 3 pieces of sepals, 2 pieces
Petal and 1 piece of lip composition of specialization；Lip 3 splits, and has typhloscle piece above；Stamen forms gynostemium, gynostemium with style symphysis
There is a flower pesticide on top, and there is a column cap depression front upper place.There are the root-like stock of thick thickness, such as chicken head shape, rich viscosity, containing bletilla in underground
Colloid, i.e. bletilla mannan, can hyoscine, have hemostasis tonifying lung, the effect of myogenic pain relieving, also be available for making thickener.
The artificial cultivation of the bletilla striata generally comprises tissue-cultured seedling culture, domesticating and cultivating and field-transplanting, and domesticating and cultivating is tissue-cultured seedling
To the transition stage between field-transplanting after cultivation, relative to the conventional hardening said, main purpose is that the growth for improving tissue-cultured seedling is fitted
Should be able to power, final raising field production survival rate, usually in the completion in greenhouse.At present, bletilla tissue culture seedlings domesticating and cultivating makes
Matrix be generally in perlite, peat soil, turf, river sand etc. it is several mix, due to the water requirement of bletilla striata growth
Very big, the moisture in matrix will be maintained at 60% to 70%, therefore, each irrigation amount is larger, and the water capacity easily accumulation is in matrix
Bottom, makes the root of the bletilla striata be subject to damage or crop failure caused by waterlogging, so as to influence the normal growth of the bletilla striata.
The content of the invention
It is an object of the invention to provide a kind of domesticating cultivation method of bletilla tissue culture seedlings, it is improved by matrix,
Both enough fertilizer efficiency had been ensure that, the root system of the bletilla striata can have been avoided to be subject to damage or crop failure caused by waterlogging again, so as to improve the qualification rate of bletilla tissue culture seedlings.
The object of the present invention is achieved like this：A kind of domesticating cultivation method of bletilla tissue culture seedlings, comprises the following steps：
A, matrix prepare：
A1, by pine nut shell medicining liquid dipping 40min to 65min, pull out and dry；
A2, by fertile soil and sheep manure according to mass ratio（3~5）:1 be well mixed, then more than 120 degrees Celsius under conditions of sterilization
60min to 90min, finally adds water and stirs, and the mass fraction for making water is 60% to 70%；
A3, by sawdust, chicken manure, soya-bean cake, fragrant bottom sludge and water according to（8~10）：（3~5）:1:1：5 mass ratio uniformly mixes, dress
In the container of sealing, the retting 30 days to 45 days under conditions of 30 DEG C to 45 DEG C, taking-up rubs broken, then is exposed to the sun in the sun 3 days
To 5 days, then spray disinfectant carried out disinfection, and finally adds water and stirs, and the mass fraction for making water is 60% to 70%；
Dig multiple cultivating pools on B, the ground in greenhouse, the width of cultivating pool is 80cm to 120cm, depth be 30cm extremely
40cm；
C, addition cultivation matrix：Cultivating pool bottom lay one layer of pine nut shell obtained through step A1, laying depth be 6cm extremely
12cm；The mixture of the fertile soil obtained through step A2 in the top laying of pine nut shell and sheep manure, laying depth be 10cm extremely
20cm；The mixture obtained through step A3 is laid above the mixture of fertile soil and sheep manure, laying depth is 5cm to 10cm；
D, selection protocorm are sturdy, well-grown bletilla tissue culture seedlings, are cleaned its root with water, then its root is placed on antibacterial
45min to 60min is soaked in agent, taking-up is dried；
E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；
F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX is extremely
3000LX, the daily 10h to 13h of light application time；Periodically water, the mass fraction of matrix reclaimed water is maintained at 60% to 70%；3 months
Crop field can be transplanted afterwards.
Further, in step A1 and A3, thimerosal use mass concentration for 0.3% liquor potassic permanganate.
Further, in step D, bacteriostatic agent uses carbendazim.
Further, in step C, after matrix all lays, spray disinfectant carries out secondary sterilization.
Further, in step F, every two weeks, use mass concentration for 0.7% thiophanate methyl spray solution
Bletilla striata blade, for preventing and treating black spot.
The beneficial effects of the invention are as follows：
1st, pine nut shell volume is larger, and each other with gap, when irrigation amount is excessive, water flows into cultivating pool by pine nut shell
Bottom, it is to avoid the root of the bletilla striata is subject to damage or crop failure caused by waterlogging, it is ensured that bletilla tissue culture seedlings normal growth.
2nd, sawdust has the function of loose, ventilative water suction moisturizing, can reduce water consumption.Sawdust and chicken manure, soya-bean cake, perfume (or spice)
There is very strong fertilizer efficiency after dregs of fat mixing retting, meanwhile, the soil is porous for fertile soil, and sheep manure also has stronger fertilizer efficiency, San Zhe
Bletilla tissue culture seedlings provide enough fertility during taming, it is not necessary to apply the growth that other organic fertilizers just disclosure satisfy that the bletilla striata again
Need, so that final-period management more facilitates.
3rd, various matrix and bletilla tissue culture seedlings are processed by sterilizing, it is ensured that the harmless growing environment of cleaning.
To sum up, bletilla tissue culture seedlings are tamed using this method, ensure that tissue-cultured seedling grows well, meet crop field
Transplant desired ratio to greatly increase, improve the qualification rate of tissue-cultured seedling.
Specific embodiment
With reference to embodiment, the present invention is further described.
Embodiment one
A kind of domesticating cultivation method of bletilla tissue culture seedlings of the invention, comprises the following steps：
A, matrix prepare：
A1, liquor potassic permanganate that pine nut shell mass concentration is 0.3% is soaked into 40min, pull out and dry；
A2, by fertile soil and sheep manure according to mass ratio 3:1 is well mixed, then the 60min that sterilized under conditions of 120 degrees Celsius, most
After add water and stir, the mass fraction for making water is 60% to 70%；
A3, by sawdust, chicken manure, soya-bean cake, fragrant bottom sludge and water according to 8：3:1:1：5 mass ratio uniformly mixes, mounted in the appearance of sealing
In device, retting 30 days under conditions of 30 DEG C, taking-up rubs broken, then is exposed to the sun in the sun 3 days, and it is 0.3% then to spray mass concentration
Liquor potassic permanganate carry out disinfection, finally add water and stir, make water mass fraction be 60% to 70%；
Multiple cultivating pools are dug on B, the ground in greenhouse, the width of cultivating pool is 80cm, and depth is 35cm；
C, addition cultivation matrix：One layer of pine nut shell obtained through step A1 is laid in the bottom of cultivating pool, laying depth is 6cm；
The fertile soil and the mixture of sheep manure obtained through step A2 in the top laying of pine nut shell, laying depth is 20cm；In fertile soil
The mixture obtained through step A3 with the mixture top laying of sheep manure, laying depth is 5cm；After all laying, sprinkling disappears
Venom carries out secondary sterilization.
D, selection protocorm are sturdy, well-grown bletilla tissue culture seedlings, are cleaned its root with water, then its root is placed on
45min is soaked in carbendazim solution, taking-up is dried；
E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；
F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX is extremely
3000LX, the daily 10h to 13h of light application time；Periodically water, the mass fraction of matrix reclaimed water is maintained at 60% to 70%；Every
Two weeks, use mass concentration for 0.7% thiophanate methyl spray solution bletilla striata blade, to prevent and treat black spot.After 3 months i.e.
Crop field can be transplanted.
The present embodiment, 10000 tissue-cultured seedling of selection carry out domesticating and cultivating, are met the tissue-cultured seedling of field-transplanting requirement
8895, qualification rate is about 89%.
Embodiment two
A kind of domesticating cultivation method of bletilla tissue culture seedlings of the invention, comprises the following steps：
A, matrix prepare：
A1, liquor potassic permanganate that pine nut shell mass concentration is 0.3% is soaked into 50min, pull out and dry；
A2, by fertile soil and sheep manure according to mass ratio 4:1 be well mixed, then more than 140 degrees Celsius under conditions of sterilization
75min, finally adds water and stirs, and the mass fraction for making water is 60% to 70%；
A3, by sawdust, chicken manure, soya-bean cake, fragrant bottom sludge and water according to 9：4:1:1：5 mass ratio uniformly mixes, mounted in the appearance of sealing
In device, retting 40 days under conditions of 40 DEG C, taking-up rubs broken, then is exposed to the sun in the sun 4 days, and it is 0.3% then to spray mass concentration
Liquor potassic permanganate carry out disinfection, finally add water and stir, make water mass fraction be 60% to 70%；
Multiple cultivating pools are dug on B, the ground in greenhouse, the width of cultivating pool is 100cm, and depth is 30cm；
C, addition cultivation matrix：One layer of pine nut shell obtained through step A1 is laid in the bottom of cultivating pool, laying depth is 8cm；
The fertile soil and the mixture of sheep manure obtained through step A2 in the top laying of pine nut shell, laying depth is 15cm；In fertile soil
The mixture obtained through step A3 with the mixture top laying of sheep manure, laying depth is 7cm；After all laying, sprinkling disappears
Venom carries out secondary sterilization.
D, selection protocorm are sturdy, well-grown bletilla tissue culture seedlings, are cleaned its root with water, then its root is placed on
50min is soaked in carbendazim solution, taking-up is dried；
E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；
F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX is extremely
3000LX, the daily 10h to 13h of light application time；Periodically water, the mass fraction of matrix reclaimed water is maintained at 60% to 70%；Every
Two weeks, use mass concentration for 0.7% thiophanate methyl spray solution bletilla striata blade, to prevent and treat black spot.After 3 months i.e.
Crop field can be transplanted.
The present embodiment, 10000 tissue-cultured seedling of selection carry out domesticating and cultivating, are met the tissue-cultured seedling of field-transplanting requirement
9107, qualification rate is about 91%.
Embodiment three
A kind of domesticating cultivation method of bletilla tissue culture seedlings of the invention, comprises the following steps：
A, matrix prepare：
A1, liquor potassic permanganate that pine nut shell mass concentration is 0.3% is soaked into 65min, pull out and dry；
A2, by fertile soil and sheep manure according to mass ratio 5:1 is well mixed, then the 90min that sterilized under conditions of 150 degrees Celsius, most
After add water and stir, the mass fraction for making water is 60% to 70%；
A3, by sawdust, chicken manure, soya-bean cake, fragrant bottom sludge and water according to 10：5:1:1：5 mass ratio uniformly mixes, mounted in the appearance of sealing
In device, retting 45 days under conditions of 45 DEG C, taking-up rubs broken, then is exposed to the sun in the sun 5 days, and it is 0.3% then to spray mass concentration
Liquor potassic permanganate carry out disinfection, finally add water and stir, make water mass fraction be 60% to 70%；
Multiple cultivating pools are dug on B, the ground in greenhouse, the width of cultivating pool is 120cm, and depth is 40cm；
C, addition cultivation matrix：One layer of pine nut shell obtained through step A1 is laid in the bottom of cultivating pool, laying depth is 12cm；
The fertile soil and the mixture of sheep manure obtained through step A2 in the top laying of pine nut shell, laying depth is 10cm；In fertile soil
The mixture obtained through step A3 with the mixture top laying of sheep manure, laying depth is 10cm；After all laying, sprinkling disappears
Venom carries out secondary sterilization.
D, selection protocorm are sturdy, well-grown bletilla tissue culture seedlings, are cleaned its root with water, then its root is placed on
60min is soaked in carbendazim solution, taking-up is dried；
E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；
F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX is extremely
3000LX, the daily 10h to 13h of light application time；Periodically water, the mass fraction of matrix reclaimed water is maintained at 60% to 70%；Every
Two weeks, use mass concentration for 0.7% thiophanate methyl spray solution bletilla striata blade, to prevent and treat black spot.After 3 months i.e.
Crop field can be transplanted.
The present embodiment, 10000 tissue-cultured seedling of selection carry out domesticating and cultivating, are met the tissue-cultured seedling of field-transplanting requirement
8956, qualification rate is about 89.5%.
The preferred embodiments of the present invention are the foregoing is only, is not intended to limit the invention, for the skill of this area
For art personnel, the present invention can have various modifications and variations.It is all within the spirit and principles in the present invention, made any repair
Change, equivalent, improvement etc., should be included within the scope of the present invention.
Claims (5)
1. a kind of domesticating cultivation method of bletilla tissue culture seedlings, it is characterised in that comprise the following steps：
A, matrix prepare：
A1, by pine nut shell medicining liquid dipping 40min to 65min, pull out and dry；
A2, by fertile soil and sheep manure according to mass ratio（3~5）:1 be well mixed, then more than 120 degrees Celsius under conditions of sterilization
60min to 90min, finally adds water and stirs, and the mass fraction for making water is 60% to 70%；
A3, by sawdust, chicken manure, soya-bean cake, fragrant bottom sludge and water according to（8~10）：（3~5）:1:1：5 mass ratio uniformly mixes, dress
In the container of sealing, the retting 30 days to 45 days under conditions of 30 DEG C to 45 DEG C, taking-up rubs broken, then is exposed to the sun in the sun 3 days
To 5 days, then spray disinfectant carried out disinfection, and finally adds water and stirs, and the mass fraction for making water is 60% to 70%；
Dig multiple cultivating pools on B, the ground in greenhouse, the width of cultivating pool is 80cm to 120cm, depth be 30cm extremely
40cm；
C, addition cultivation matrix：Cultivating pool bottom lay one layer of pine nut shell obtained through step A1, laying depth be 6cm extremely
12cm；The mixture of the fertile soil obtained through step A2 in the top laying of pine nut shell and sheep manure, laying depth be 10cm extremely
20cm；The mixture obtained through step A3 is laid above the mixture of fertile soil and sheep manure, laying depth is 5cm to 10cm；
D, selection protocorm are sturdy, well-grown bletilla tissue culture seedlings, are cleaned its root with water, then its root is placed on antibacterial
45min to 60min is soaked in agent, taking-up is dried；
E, the tissue-cultured seedling that will be obtained through step D are planted in the matrix in cultivating pool, line-spacing 2cm to 4cm, spacing in the rows 1cm to 2cm；
F, management：By the temperature control in greenhouse in 22 DEG C to 25 DEG C, humidity 60% to 70%；Intensity of illumination 2500LX is extremely
3000LX, the daily 10h to 13h of light application time；Periodically water, the mass fraction of matrix reclaimed water is maintained at 60% to 70%；3 months
Crop field can be transplanted afterwards.
2. the domesticating cultivation method of a kind of bletilla tissue culture seedlings according to claim 1, it is characterised in that：In step A1 and
In A3, thimerosal use mass concentration for 0.3% liquor potassic permanganate.
3. the domesticating cultivation method of a kind of bletilla tissue culture seedlings according to claim 1, it is characterised in that：It is antibacterial in step D
Agent uses carbendazim.
4. the domesticating cultivation method of a kind of bletilla tissue culture seedlings according to claim 1, it is characterised in that：In step C, base
After matter all lays, spray disinfectant carries out secondary sterilization.
5. the domesticating cultivation method of a kind of bletilla tissue culture seedlings according to claim 1,2,3 or 4, it is characterised in that：In step
In rapid F, every two weeks, use mass concentration for 0.7% thiophanate methyl spray solution bletilla striata blade.
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| CN201611045002.8A CN106718721B (en) | 2016-11-24 | 2016-11-24 | Domestication cultivation method of bletilla striata tissue culture seedlings |
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| CN201611045002.8A CN106718721B (en) | 2016-11-24 | 2016-11-24 | Domestication cultivation method of bletilla striata tissue culture seedlings |
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| CN106718721B CN106718721B (en) | 2020-02-11 |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107211676A (en) * | 2017-05-26 | 2017-09-29 | 黄山峰源生物科技有限公司 | The acclimation method of bletilla tissue culture seedlings |
| CN107241998A (en) * | 2017-06-28 | 2017-10-13 | 金寨县金绿源特色中药材种植农场 | A kind of greenhouse gardening bletilla striata final-period management method |
| CN107548914A (en) * | 2017-09-29 | 2018-01-09 | 道真自治县百草谷旅游和农业开发有限责任公司 | A kind of implantation methods of the bletilla striata |
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| CN113951118A (en) * | 2021-10-18 | 2022-01-21 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
| CN113951118B (en) * | 2021-10-18 | 2023-02-17 | 浙江省农业科学院 | Simple cultivation method for rapid seedling emergence of bletilla ochracea |
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Denomination of invention: A domestication method of Bletilla striata tissue culture seedlings Effective date of registration: 20220614 Granted publication date: 20200211 Pledgee: China Construction Bank Corporation Xuanwei sub branch Pledgor: XUANWEI NONGSHUO AGRICULTURAL SPECIAL PRODUCTS DEVELOPMENT Co.,Ltd. Registration number: Y2022980007778 |
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Denomination of invention: A method of domestication and cultivation of tissue culture seedlings of white hyacinth Granted publication date: 20200211 Pledgee: China Construction Bank Corporation Xuanwei sub branch Pledgor: XUANWEI NONGSHUO AGRICULTURAL SPECIAL PRODUCTS DEVELOPMENT Co.,Ltd. Registration number: Y2024980012954 |