CN107624394A - A kind of method for inducing hickory nut adventitious root to occur - Google Patents
A kind of method for inducing hickory nut adventitious root to occur Download PDFInfo
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- CN107624394A CN107624394A CN201710732786.XA CN201710732786A CN107624394A CN 107624394 A CN107624394 A CN 107624394A CN 201710732786 A CN201710732786 A CN 201710732786A CN 107624394 A CN107624394 A CN 107624394A
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Abstract
The invention discloses a kind of method for inducing hickory nut adventitious root to occur, including：Prepare cuttings, prepare cuttage hotbed, compound gel root-growing agent, cuttage, post processing.Have the beneficial effect that：After cuttings is soaked in gel root-growing agent, one layer of root-growing agent film can be formed on the surface of cuttings, the gel root-growing agent film of aerial part can reduce the moisture evaporation of cuttings, prevent that cuttings is withered lesion, the gel root-growing agent film of under ground portion absorbs water in cutting medium and can keep moisture state, being sustained out root-growing agent simultaneously stimulates cuttage rooting, number of taking root, root long degree and the sturdy degree of cuttings is improved, so as to improve cuttage survival rate；Film shed is built on seedbed can provide the environment of a heat and moisture preserving for nursery stock, avoid direct sunlight, reduce the moisture evaporation of nursery stock, improve cuttage survival rate, therefore the inventive method is a kind of method that simple, efficient induction hickory nut adventitious root occurs.
Description
Technical field
The invention belongs to plant establishment technical field, more particularly to a kind of method for inducing hickory nut adventitious root to occur.
Technical background
Hickory nut（Carya cathayensis Sarg.）, also known as Juglans mandshurica, juglans mandshurica.It is a kind of deciduous tree, category is recklessly
Peach section hickory, up to 10-20 rice, 30-60 centimetres of the diameter of a cross-section of a tree trunk 1.3 meters above the ground；Bark is smooth, canescence, smooth；Sprig is carefully thin, the close quilt of shoot
Peltate raw orange-yellow body of gland, and later body of gland is gradually sparse, and 1 year raw branch purplish grey, often there are sparse pubescence, skin in upper end
Hole is circular, sparse.The fruit of hickory nut is due to high nutritive value and unique taste flavor, having obtained consumer's
Accreditation, is increasingly becoming a kind of very popular high-grade nut.China is suitable to be born in the foot of a mountain sparse woods or humus one of for original producton location
Abundant mountain valley.Walnut kernel is delicious edible, and containing protein 7.23%, oil content 48%~53%, up to 69%, walnut kernel contains more persons
Unrighted acid necessary to more protein and human nutrition.
The production of China hickory nut nursery stock at present mainly uses seed sowing and propagation by grafiting.Seminal propagation survival rate is high,
But import must be relied on, costly, the nursery stock variation of cultivation is big, solid slow for price, is generally used for that stock is afforested or cultivated with material.
Propagation by grafiting can effectively shorten the time limit of just bearing fruit, but need substantial amounts of stock and scion, and time consuming, cost is higher.Cuttage is numerous
Grow with convenient material drawing, the seedling cycle is short, the advantages that keeping the merit of parent, but hickory is bred in cuttage is difficult
Seeds, research of the China in terms of hickory nut cutting propagation is seldom, and successfully report is few for cuttage, is primarily due to hickory nut and inserts
Fringe, which is not easy to take root, causes its cuttage life rate not high.
The technology occurred on induction hickory nut adventitious root has many methods, and as applied, public number is CN to prior art
103238492 B Chinese invention patent, disclose a kind of method for promoting apocarya branch adventitious root to occur, the invention
Method chooses annual apocarya branch as cuttings, inserts the perlite being layered on above heating wire, peat soil, river
In the matrix that husky, four kinds of matrix of vermiculite mix in proportion, cuttings is soaked with rooting powder solution before cuttage, has cuttage letter
The advantages of single, suitable large-scale production, but the easy photooxidative degradation of main matter of the root-inducing powder in the inventive method, so as to
Make the reduction of its rooting effect, antibiotic and sterilizing processing is not carried out to cuttings wound, easily causes cuttings to be rotted mouldy, reduce cuttage
Survival rate, and in the inventive method, the nursery stock after cuttage does not carry out sun-shading, easily causes the moisture of cuttings to dissipate
Lose, and then influence the growth of nursery stock.
The content of the invention
The purpose of this method is to provide a kind of method for inducing hickory nut adventitious root to occur, and this method is simple to operate, fits
Large-scale production is closed, adventitious root incidence is higher, and plant percent is higher.
The present invention is directed to the problem of being mentioned in background technology, and the technical scheme taken is：One kind induction hickory nut adventitious root
The method of generation, including：Prepare cuttings, prepare cuttage hotbed, compound gel root-growing agent, cuttage, post processing, specifically include following
Step：
Prepare cuttings：Annual hickory nut branch is taken, the cuttings of 10-15cm length is cut into, is put down on otch oblique, it is ensured that each section is inserted
There are 2-3 full buds on fringe, upper cut at 2-4cm, crack-free, the bundled the moon of cuttings is being hidden above bud；Annual cuttings
Rooting rate is higher, and lucifuge preservation is advantageous to prevent the water loss of cuttings, improves cuttage survival rate；
Prepare cuttage hotbed：Thick 2-3cm microlith is spread after slotting machine bottom is smooth, is then covered with thick 1-2cm straw, and uniformly
Thick 2-3cm perlite is spread, ground hot line is uniformly laid in U-typed on perlite, spacing 10-12cm, then lays
Plugging soil thick 8.0-10.0cm, matrix used are degerming by sterilizing；The setting of cuttage hotbed can provide one for cuttings
The growing environment of heat and moisture preserving, accelerate cuttage rooting；
Compound gel root-growing agent：Accurately weigh heteroauxin 0.2-0.3 parts, indolebutyric acid 0.6-0.7 parts, methyl α-naphthyl acetate 0.9-1.0
Part, functional polypeptide 0.01-0.02 parts, vitamin B12 0.01-0.02 parts, aspirin 0.10-0.15 parts, potassium dihydrogen phosphate
0.20-0.25 parts, the stirring of distilled water 20-25 parts lucifuge are thoroughly mixed, and obtain cuttage root-taking agent；6-8wt.% is equipped with normal temperature condition
Polyvinyl alcohol water solution 850-950 parts, add glycerine 1.2-2.2 parts 30-50 minutes, temperature are reacted at a temperature of 40-50 DEG C
30-40 DEG C is down to, forms hydrogel；Then cuttage root-taking agent is added under slow stirring, treats that solution reaction is uniformly made solidifying
Glue root-growing agent；The amino acid sequence of functional polypeptide is：SCASRCFKSCRAKSRCRARRCRARRCGCRARRCGYYVSVFRC；
With heteroauxin, indolebutyric acid, methyl α-naphthyl acetate complex reaction can occur for functional polypeptide, complex compound be generated, so as to avoid
Auxin is indoles and aldehyde by photooxidative degradation, ensure that the realization of auxin growth-promoting root growth promotion function；Root-growing agent can be to prevent
The only withered lesion of cuttings, the disease-resistant antibacterial ability of cuttings tissue is improved, rooting rate and quality of rooting are improved, so as to improve cuttage
Success rate；
Cuttage：Cuttings training is put into gel root-growing agent takes out after the immersion 5-8 seconds, repeats that cuttage can be implemented 2-3 times；Before cuttage
With wooden stick exploratory heading, cuttage depth is the 2/3-3/4 of cutting length, and strain spacing is 10-12cm, line space 15-18cm, after cuttage
It is real with hand pressure, pour water；After cuttings is soaked in gel root-growing agent, one layer of root-growing agent film can be formed on the surface of cuttings, on the ground
Partial gel root-growing agent film can reduce the moisture evaporation of cuttings, prevent that cuttings is withered lesion, the gel of under ground portion is taken root
Agent film absorbs water in cutting medium can keep moisture state, while be sustained out root-growing agent stimulation cuttage rooting, improve the life of cuttings
Radical, root long degree and sturdy degree, so as to improve cuttage survival rate；
Post processing：Circular membrane shed is built after cuttage above seedbed, was watered 1 time every 3-5 days after inserting, it is every after cuttage 20 days
Bacteria-treating is carried out every 7-8 days spraying 0.08-0.10wt.% carbendazim, film, and daily injection spray pipe are raised after cutting lamina
Reason, transplantation of seedlings can be played within 50-60 days after inserting；Film shed is built on seedbed can provide the ring of a heat and moisture preserving for nursery stock
Border, direct sunlight is avoided, reduce the moisture evaporation of nursery stock, accelerated nursery stock and take root, improve cuttage survival rate.
Preferably, it is 3-4 that plugging soil used, which is volume ratio,：4-5:1-3 furrow soil, vermiculite, turfy soil mixed-matrix；Insert
Contain the nutriment such as middle trace element, humus, organic matter necessary to seedling growth in earth, coordinate root-growing agent to promote nursery stock
Growth and breeding.
Compared with prior art, the advantage of the invention is that：1）Functional polypeptide in root-growing agent can avoid auxin
By photooxidative degradation, the somatotrophic effect of auxin growth-promoting root ensure that, root-growing agent can prevent that cuttings is withered lesion, is improved and is inserted
The disease-resistant antibacterial ability of fringe tissue, rooting rate and quality of rooting are improved, so as to improve cuttage success rate；2）Cuttings is given birth in gel
After being soaked in root agent, one layer of root-growing agent film can be formed on the surface of cuttings, the gel root-growing agent film of aerial part can reduce slotting
The moisture evaporation of fringe, prevent that cuttings is withered lesion, the gel root-growing agent film of under ground portion absorbs water in cutting medium and can keep wet
Profit state, while root-growing agent stimulation cuttage rooting is sustained out, number of taking root, root long degree and the sturdy degree of cuttings are improved, so as to improve
Cuttage survival rate；3）Film shed is built on seedbed can provide the environment of a heat and moisture preserving for nursery stock, avoid sunlight straight
Penetrate, reduce the moisture evaporation of nursery stock, improve cuttage survival rate, therefore the inventive method is a kind of simple, efficient induction mountain core
The method that peach adventitious root occurs.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of method for inducing hickory nut adventitious root to occur, comprises the following steps：
1）Annual hickory nut branch is taken, the cuttings of 10cm length is cut into, is put down on otch oblique, it is ensured that there are 2 in each section of cuttings
Full bud, upper cut at 2cm, crack-free, the bundled the moon of cuttings are being hidden above bud；Spread thick 2cm's after slotting machine bottom is smooth
Microlith, is then covered with thick 1cm straw, and uniformly spreads thick 2cm perlite, uniformly lays ground in U-typed on perlite
Hot line, spacing 10cm, the thick plugging soils of 8.0cm are then laid, plugging soil is that volume ratio is 3：5:1 furrow soil, vermiculite, turfy soil mix
Close matrix；Matrix used is degerming by sterilizing；The setting of cuttage hotbed can provide the life of a heat and moisture preserving for cuttings
Long environment, accelerate cuttage rooting；
2）Accurately weigh 0.2 part of heteroauxin, 0.6 part of indolebutyric acid, 0.9 part of methyl α-naphthyl acetate, 0.01 part of functional polypeptide, vitamin
20 parts of 0.01 part of B12,0.10 part of aspirin, 0.20 part of potassium dihydrogen phosphate, distilled water lucifuge stirrings are thoroughly mixed, and obtain cuttage
Root-growing agent；6wt.% 850 parts of polyvinyl alcohol water solution is equipped with normal temperature condition, 1.2 parts of glycerine is added and is reacted at a temperature of 40 DEG C
30 minutes, temperature was down to 30 DEG C, formed hydrogel；Then cuttage root-taking agent is added under slow stirring, treats that solution reaction is uniform
Gel root-growing agent is made；The amino acid sequence of functional polypeptide is：
SCASRCFKSCRAKSRCRARRCRARRCGCRARRCGYYVSVFRC；Functional polypeptide can with heteroauxin, indolebutyric acid,
Complex reaction occurs for methyl α-naphthyl acetate, generates complex compound, by photooxidative degradation is indoles and aldehyde so as to avoid auxin, ensure that
The realization of auxin growth-promoting root growth promotion function；
3）Cuttings training be put into soaked 5 seconds in gel root-growing agent after take out, cuttage can be implemented by being repeated 2 times；With wooden stick before cuttage
Exploratory heading, cuttage depth are the 2/3 of cutting length, and strain spacing is 10cm, line space 15cm, real with hand pressure after cuttage, pour water；
4）Circular membrane shed is built after cuttage above seedbed, was watered 1 time every 3 days after inserting, cuttage was sprayed after 20 days every 7 days
Mist 0.08wt.% carbendazim carries out bacteria-treating, film, and spraying management daily is raised after cutting lamina, 50 days after inserting
Play transplantation of seedlings；Film shed is built on seedbed can provide the environment of a heat and moisture preserving for nursery stock, avoid direct sunlight, drop
The moisture evaporation of low nursery stock, accelerate nursery stock and take root, improve cuttage survival rate.
Embodiment 2：
A kind of method for inducing hickory nut adventitious root to occur, comprises the following steps：
1）Prepare cuttings：Annual hickory nut branch is taken, the cuttings of 15cm length is cut into, is put down on otch oblique, it is ensured that each section is inserted
There are 3 full buds on fringe, upper cut at 4cm, crack-free, the bundled the moon of cuttings is being hidden above bud；Annual cuttings is taken root
Rate is higher, and lucifuge preservation is advantageous to prevent the water loss of cuttings, improves cuttage survival rate；
2）Prepare cuttage hotbed：Thick 3cm microlith is spread after slotting machine bottom is smooth, is then covered with thick 2cm straw, and uniformly spread
Upper thick 3cm perlite, ground hot line is uniformly laid in U-typed on perlite, spacing 12cm, then lays 10.0cm thickness
Plugging soil, plugging soil are that volume ratio is 4：4:3 furrow soil, vermiculite, turfy soil mixed-matrix；Matrix used is degerming by sterilizing；
The setting of cuttage hotbed can provide the growing environment of a heat and moisture preserving for cuttings, accelerate cuttage rooting；
3）Compound gel root-growing agent：It is more accurately to weigh 0.3 part of heteroauxin, 0.7 part of indolebutyric acid, 1.0 parts of methyl α-naphthyl acetate, feature
25 parts of 0.02 part of peptide, 0.02 part of vitamin B12,0.15 part of aspirin, 0.25 part of potassium dihydrogen phosphate, distilled water lucifuge stirrings
It is thoroughly mixed, obtains cuttage root-taking agent；8wt.% 950 parts of polyvinyl alcohol water solution is equipped with normal temperature condition, adds 2.2 parts of glycerine
Reacted 50 minutes at a temperature of 50 DEG C, temperature is down to 40 DEG C, forms hydrogel；Then cuttage root-taking is added under slow stirring
Agent, treat that gel root-growing agent is uniformly made in solution reaction；Root-growing agent can prevent that cuttings is withered lesion, improve the anti-of cuttings tissue
Sick antibacterial ability, rooting rate and quality of rooting are improved, so as to improve cuttage success rate；
4）Cuttage：Cuttings training be put into soaked 8 seconds in gel root-growing agent after take out, cuttage can be implemented by being repeated 3 times；Before cuttage with
Wooden stick exploratory heading, cuttage depth are the 3/4 of cutting length, and strain spacing is 12cm, line space 18cm, real with hand pressure after cuttage, are poured
Sufficient water；Circular membrane shed is built after cuttage above seedbed, was watered 1 time every 5 days after inserting, cuttage was sprayed after 20 days every 8 days
Mist 0.10wt.% carbendazim carries out bacteria-treating, film, and spraying management daily is raised after cutting lamina, 60 days after inserting
Play transplantation of seedlings；Film shed is built on seedbed can provide the environment of a heat and moisture preserving for nursery stock, avoid direct sunlight, drop
The moisture evaporation of low nursery stock, accelerate nursery stock and take root, improve cuttage survival rate.
Embodiment 3：
A kind of method for inducing hickory nut adventitious root to occur, including：Prepare cuttings, prepare cuttage hotbed, compound gel root-growing agent,
Cuttage, post processing, specifically include following steps：
Prepare cuttings：Annual hickory nut branch is taken, the cuttings of 15cm length is cut into, is put down on otch oblique, it is ensured that each section of cuttings
On have 3 full buds, upper cut is above bud at 3cm, crack-free, cloudy hides cuttings is bundled；The rooting rate of annual cuttings
Higher, lucifuge preservation is advantageous to prevent the water loss of cuttings, improves cuttage survival rate；
Prepare cuttage hotbed：Thick 3cm microlith is spread after slotting machine bottom is smooth, is then covered with thick 2cm straw, and uniformly spread
Thick 2cm perlite, ground hot line is uniformly laid in U-typed on perlite, spacing 12cm, then lays inserting for 10.0cm thickness
Earth, plugging soil are that volume ratio is 3：5:1 furrow soil, vermiculite, turfy soil mixed-matrix；Matrix used is degerming by sterilizing；Skewer
The growing environment of a heat and moisture preserving can be provided for cuttings by inserting the setting of hotbed, accelerate cuttage rooting；
Compound gel root-growing agent：Accurately weigh 0.2 part of heteroauxin, 0.7 part of indolebutyric acid, 1.0 parts of methyl α-naphthyl acetate, functional polypeptide
0.01 part, 0.01 part of vitamin B12,0.10 part of aspirin, 25 parts of 0.20 part of potassium dihydrogen phosphate, distilled water lucifuges have stirred
Full mixing, obtains cuttage root-taking agent；8wt.% 950 parts of polyvinyl alcohol water solution is equipped with normal temperature condition, 2.2 parts of glycerine is added and exists
Reacted 50 minutes at a temperature of 50 DEG C, temperature is down to 40 DEG C, forms hydrogel；Then cuttage root-taking agent is added under slow stirring,
Treat that gel root-growing agent is uniformly made in solution reaction；The amino acid sequence of functional polypeptide is：
SCASRCFKSCRAKSRCRARRCRARRCGCRARRCGYYVSVFRC；Functional polypeptide can with heteroauxin, indolebutyric acid,
Complex reaction occurs for methyl α-naphthyl acetate, generates complex compound, by photooxidative degradation is indoles and aldehyde so as to avoid auxin, ensure that
The realization of auxin growth-promoting root growth promotion function；Root-growing agent can prevent that cuttings is withered lesion, improve the disease-resistant anti-of cuttings tissue
Bacterium ability, rooting rate and quality of rooting are improved, so as to improve cuttage success rate；
Cuttage：Cuttings training be put into soaked 7 seconds in gel root-growing agent after take out, cuttage can be implemented by being repeated 2 times；With wood before cuttage
Rod exploratory heading, cuttage depth are the 2/3 of cutting length, and strain spacing is 12cm, line space 15cm, real with hand pressure after cuttage, are poured
Water；After cuttings is soaked in gel root-growing agent, one layer of root-growing agent film can be formed on the surface of cuttings, the gel of aerial part is taken root
Agent film can reduce the moisture evaporation of cuttings, prevent that cuttings is withered lesion, the gel root-growing agent film of under ground portion is in cutting medium
Middle water suction can keep moisture state, while be sustained out root-growing agent and stimulate cuttage rooting, improve the number of taking root of cuttings, root long degree with it is thick
Strong degree, so as to improve cuttage survival rate；
Post processing：Circular membrane shed is built after cuttage above seedbed, was watered 1 time every 4 days after inserting, after cuttage 20 days every
Spraying 0.10wt.% carbendazim carries out bacteria-treating within 7 days, film, and spraying management daily is raised after cutting lamina, 60 after inserting
It can play transplantation of seedlings；Film shed is built on seedbed can provide the environment of a heat and moisture preserving for nursery stock, avoid sunlight
Direct projection, the moisture evaporation of nursery stock is reduced, accelerate nursery stock and take root, improve cuttage survival rate.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail embodiment described above, it should be understood that it is described above only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should be included in the scope of the protection.
Sequence table
<110>Close big vast gardening research and development Co., Ltd in Pujiang County
<120>A kind of method for inducing hickory nut adventitious root to occur
<160> 1
<170> SIPOSequenceListing 1.0
<210> 1
<211> 42
<212> PRT
<213>Artificial synthesized (Mytilus coruscus)
<400> 1
Ser Cys Ala Ser Arg Cys Phe Lys Ser Cys Arg Ala Lys Ser Arg Cys
1 5 10 15
Arg Ala Arg Arg Cys Arg Ala Arg Arg Cys Gly Cys Arg Ala Arg Arg
20 25 30
Cys Gly Tyr Tyr Val Ser Val Phe Arg Cys
35 40
Claims (8)
1. a kind of method for inducing hickory nut adventitious root to occur, including：Cuttings, preparation cuttage hotbed, compound gel is prepared to take root
Agent, cuttage, post processing, it is characterised in that：Described compound gel root-growing agent step is：It is equipped with 6-8wt.%'s under normal temperature condition
Polyvinyl alcohol water solution 850-950 parts, add glycerine 1.2-2.2 parts and reacted at a temperature of 40-50 DEG C 30-50 minutes, temperature drop
To 30-40 DEG C, hydrogel is formed；Then cuttage root-taking agent is added under slow stirring, treats that gel is uniformly made in solution reaction
Root-growing agent.
A kind of 2. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described preparation
Cuttings step is：Annual hickory nut branch is taken, the cuttings of 10-15cm length is cut into, is put down on otch oblique, it is ensured that each section of cuttings
On have a full buds of 2-3, upper cut is above bud 2-4cm at, crack-free, by the bundled cloudy Tibetan of cuttings.
A kind of 3. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described preparation
Cuttage hotbed step is：Thick 2-3cm microlith is spread after slotting machine bottom is smooth, is then covered with thick 1-2cm straw, and uniformly spread
Upper thick 2-3cm perlite, ground hot line is uniformly laid in U-typed on perlite, spacing 10-12cm, then lays 8.0-
Plugging soil thick 10.0cm, matrix used are degerming by sterilizing.
A kind of 4. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described preparation
Plugging soil in cuttage hotbed step is：Volume ratio is 3-4：4-5:1-3 furrow soil, vermiculite, turfy soil mixed-matrix.
A kind of 5. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described compounding
Gel root-growing agent step is：It is accurate weigh heteroauxin 0.2-0.3 parts, indolebutyric acid 0.6-0.7 parts, methyl α-naphthyl acetate 0.9-1.0 parts,
Functional polypeptide 0.01-0.02 parts, vitamin B12 0.01-0.02 parts, aspirin 0.10-0.15 parts, potassium dihydrogen phosphate
0.20-0.25 parts, the stirring of distilled water 20-25 parts lucifuge are thoroughly mixed, and obtain cuttage root-taking agent；6-8wt.% is equipped with normal temperature condition
Polyvinyl alcohol water solution 850-950 parts, add glycerine 1.2-2.2 parts 30-50 minutes, temperature are reacted at a temperature of 40-50 DEG C
30-40 DEG C is down to, forms hydrogel；Then cuttage root-taking agent is added under slow stirring, treats that solution reaction is uniformly made solidifying
Glue root-growing agent.
A kind of 6. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described compounding
The amino acid sequence of functional polypeptide in gel root-growing agent step is：SCASRCFKSCRAKSRCRARRCRARRCGCRARRCG
YYVSVFRC。
A kind of 7. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described cuttage
Step is：Cuttings training is put into gel root-growing agent takes out after the immersion 5-8 seconds, repeats that cuttage can be implemented 2-3 times；Before cuttage with
Wooden stick exploratory heading, cuttage depth be cutting length 2/3-3/4, strain spacing is 10-12cm, line space 15-18cm, after cuttage with
Hand pressure is real, pours water.
A kind of 8. method for inducing hickory nut adventitious root to occur according to claim 1, it is characterised in that：Described rear place
Managing step is：Circular membrane shed is built after cuttage above seedbed, was watered 1 time every 3-5 days after inserting, after cuttage 20 days every
Spraying 0.08-0.10wt.% carbendazim carries out bacteria-treating within 7-8 days, and film, and spraying management daily are raised after cutting lamina,
Transplantation of seedlings can be played after inserting within 50-60 days.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108207367A (en) * | 2018-02-10 | 2018-06-29 | 宜州市横山泰顺种养专业合作社 | A kind of cuttage and seedling culture method of passion fruit |
| CN108966879A (en) * | 2018-08-09 | 2018-12-11 | 兴义市天瑞核桃种植农民专业合作社 | A kind of method of hickory nut cuttage and seedling culture |
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| CN103651366A (en) * | 2013-12-10 | 2014-03-26 | 凉山华森林业科技有限公司 | Additive capable of resisting bacteria and preserving moisture, and method used for processing cutting branches with additive |
| CN103999706A (en) * | 2014-06-24 | 2014-08-27 | 南京林业大学 | Method for increasing cutting rooting rate of pecans |
| CN105052563A (en) * | 2015-08-05 | 2015-11-18 | 浙江农林大学 | Composite seedling culturing method of cutting and grafting hard branches of carya illinoensis k. koch at the same period |
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| CN108207367A (en) * | 2018-02-10 | 2018-06-29 | 宜州市横山泰顺种养专业合作社 | A kind of cuttage and seedling culture method of passion fruit |
| CN108966879A (en) * | 2018-08-09 | 2018-12-11 | 兴义市天瑞核桃种植农民专业合作社 | A kind of method of hickory nut cuttage and seedling culture |
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