CN101365722A - 纯化抗Aβ抗体的方法 - Google Patents
纯化抗Aβ抗体的方法 Download PDFInfo
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- CN101365722A CN101365722A CNA2006800216391A CN200680021639A CN101365722A CN 101365722 A CN101365722 A CN 101365722A CN A2006800216391 A CNA2006800216391 A CN A2006800216391A CN 200680021639 A CN200680021639 A CN 200680021639A CN 101365722 A CN101365722 A CN 101365722A
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/395—Antibodies; Immunoglobulins; Immune serum, e.g. antilymphocytic serum
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
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Abstract
Description
梯度时间 | %A | %B |
0-1 | 95 | 5 |
2 | 70 | 30 |
54 | 60 | 40 |
55.1-70 | 95 | 5 |
运行号 | 条件 | %LMW | %IRT |
1 | 对照(无洗涤1) | 4.4 | 2.5 |
2 | 20%丙二醇,pH7.5 | 4.7 | 2.5 |
3 | 50mM Tris,2.0M氯化镁,pH7.5 | 1.6 | 1.5 |
4 | 50mM Tris,2.5M氯化镁,pH7.5 | 1.5 | 1.3 |
5 | 50mM 乙酸酯,2.0M氯化镁,pH4.5 | 0.9 | 0.8 |
6 | 50mM Tris,4.0M氯化钠,pH7.5 | 4.4 | 2.5 |
7 | 50mM Tris,2.0M氯化钙,pH7.5 | 1.8 | 1.4 |
8 | 50mM Tris,2.5M氯化钙,pH7.5 | 0.8 | 0.8 |
运行号 | 洗涤1的缓冲液 | % IRT |
1 | 50mM Tris,2MCaCl2,pH7.5 | 0.8 |
2 | 对照(无) | 1.9 |
运行号 | 洗涤1 | 洗涤2 | DNA(ng/mL) | DNA(ppm) |
1 | 无(对照) | 20mM Tris,1M NaCl,pH 7.5 | 3.6 | 0.37 |
2 | 50mM Tris,2MCaCl2,pH 7.5 | 无 | 0.9 | 0.09 |
3 | 50mM Tris,2MCaCl2,pH 7.5 | 20mM Tris,1M NaCl,pH 7.5 | 0.3 | 0.03 |
运行号 | 样品 | %UDB |
1 | 50mM醋酸盐,2.0M CaCl2,pH 5.0 | 9.5 |
2 | 无(对照) | 20.8 |
运行号 | 洗涤1的条件 | HCP(PPM) |
1 | 50mM Tris,1.0mM NaCl,pH 7.5 | 17,600 |
2 | 50mM乙酸钠,1.5mM MnCl2,pH 5.0* | 10,600 |
3 | 50mM乙酸钠,1.5mM NiCl2,pH 5.0* | 4.700 |
4 | 50mM Tris,2.0mM CaCl2,pH 7.5 | 6,500 |
运行号 | 洗涤1的条件 | IRT(%) |
1 | 50mM Tris,1.0mM NaCl,pH 7.5 | 2.78 |
2 | 50mM乙酸钠,1.5mM MnCl2,pH 5.0* | 0.77 |
3 | 50mM乙酸钠,1.5mM NiCl2,pH 5.0* | 0.47 |
4 | 50mM Tris,2.0mM CaCl2,pH 7.5 | 0.87 |
Claims (45)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US69182105P | 2005-06-17 | 2005-06-17 | |
US60/691,821 | 2005-06-17 |
Publications (1)
Publication Number | Publication Date |
---|---|
CN101365722A true CN101365722A (zh) | 2009-02-11 |
Family
ID=37433887
Family Applications (3)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510211982.3A Active CN104926938B (zh) | 2005-06-17 | 2006-06-16 | 纯化含Fc区蛋白的方法 |
CNA200680021590XA Pending CN101213211A (zh) | 2005-06-17 | 2006-06-16 | 纯化含Fc区蛋白的方法 |
CNA2006800216391A Pending CN101365722A (zh) | 2005-06-17 | 2006-06-16 | 纯化抗Aβ抗体的方法 |
Family Applications Before (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201510211982.3A Active CN104926938B (zh) | 2005-06-17 | 2006-06-16 | 纯化含Fc区蛋白的方法 |
CNA200680021590XA Pending CN101213211A (zh) | 2005-06-17 | 2006-06-16 | 纯化含Fc区蛋白的方法 |
Country Status (26)
Country | Link |
---|---|
US (3) | US7825223B2 (zh) |
EP (3) | EP2388274A1 (zh) |
JP (2) | JP5150488B2 (zh) |
KR (2) | KR101307614B1 (zh) |
CN (3) | CN104926938B (zh) |
AR (2) | AR053632A1 (zh) |
AU (2) | AU2006259298B2 (zh) |
BR (2) | BRPI0611600B8 (zh) |
CA (2) | CA2611816C (zh) |
CR (2) | CR9576A (zh) |
EA (2) | EA015148B1 (zh) |
EC (1) | ECSP088086A (zh) |
ES (2) | ES2405079T3 (zh) |
HK (1) | HK1117174A1 (zh) |
IL (2) | IL187642A (zh) |
MA (1) | MA29609B1 (zh) |
MX (2) | MX2007015952A (zh) |
MY (1) | MY144484A (zh) |
NI (1) | NI200700329A (zh) |
NO (2) | NO20076082L (zh) |
NZ (1) | NZ565044A (zh) |
RU (1) | RU2415865C2 (zh) |
SG (1) | SG162806A1 (zh) |
TW (2) | TWI372763B (zh) |
WO (2) | WO2006138553A2 (zh) |
ZA (2) | ZA200710331B (zh) |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN106519029A (zh) * | 2016-10-31 | 2017-03-22 | 山东泰邦生物制品有限公司 | 一种Aβ寡聚体抗体的制备工艺 |
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CN106519029A (zh) * | 2016-10-31 | 2017-03-22 | 山东泰邦生物制品有限公司 | 一种Aβ寡聚体抗体的制备工艺 |
CN106519029B (zh) * | 2016-10-31 | 2020-03-17 | 山东泰邦生物制品有限公司 | 一种Aβ寡聚体抗体的制备工艺 |
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