MX355048B - Metodo de separacion para anticuerpos fucosilados. - Google Patents

Metodo de separacion para anticuerpos fucosilados.

Info

Publication number
MX355048B
MX355048B MX2014004604A MX2014004604A MX355048B MX 355048 B MX355048 B MX 355048B MX 2014004604 A MX2014004604 A MX 2014004604A MX 2014004604 A MX2014004604 A MX 2014004604A MX 355048 B MX355048 B MX 355048B
Authority
MX
Mexico
Prior art keywords
antibodies
separation method
fucosylated antibodies
separation
fucosylation
Prior art date
Application number
MX2014004604A
Other languages
English (en)
Other versions
MX2014004604A (es
Inventor
Umana Pablo
Freimoser-Grundschober Anne
Jaeger Christiane
Sondermann Peter
Original Assignee
Roche Glycart Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Glycart Ag filed Critical Roche Glycart Ag
Publication of MX2014004604A publication Critical patent/MX2014004604A/es
Publication of MX355048B publication Critical patent/MX355048B/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/705—Receptors; Cell surface antigens; Cell surface determinants
    • C07K14/70503—Immunoglobulin superfamily
    • C07K14/70535—Fc-receptors, e.g. CD16, CD32, CD64 (CD2314/705F)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
    • C07K16/065—Purification, fragmentation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/21—Immunoglobulins specific features characterized by taxonomic origin from primates, e.g. man
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/40—Immunoglobulins specific features characterized by post-translational modification
    • C07K2317/41—Glycosylation, sialylation, or fucosylation

Abstract

La presente invención se refiere a un método para la separación de anticuerpos, específicamente anticuerpos que tienen diferentes grados de fucosilación. El método se basa en la afinidad de unión de los anticuerpos a los receptores Fc. La invención se refiere adicionalmente al uso de receptores Fc para la separación de anticuerpos que tienen diferentes grados de fucosilación.
MX2014004604A 2011-10-19 2012-10-16 Metodo de separacion para anticuerpos fucosilados. MX355048B (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP11185798 2011-10-19
PCT/EP2012/070439 WO2013057078A1 (en) 2011-10-19 2012-10-16 Separation method for fucosylated antibodies

Publications (2)

Publication Number Publication Date
MX2014004604A MX2014004604A (es) 2014-05-27
MX355048B true MX355048B (es) 2018-04-03

Family

ID=47019025

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2014004604A MX355048B (es) 2011-10-19 2012-10-16 Metodo de separacion para anticuerpos fucosilados.

Country Status (12)

Country Link
US (2) US9994610B2 (es)
EP (1) EP2768845B1 (es)
JP (2) JP6356605B2 (es)
KR (1) KR20140077178A (es)
CN (2) CN108314729A (es)
BR (1) BR112014009178A2 (es)
CA (1) CA2851053A1 (es)
ES (1) ES2619677T3 (es)
HK (2) HK1258766A1 (es)
MX (1) MX355048B (es)
RU (1) RU2650873C2 (es)
WO (1) WO2013057078A1 (es)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8911964B2 (en) 2006-09-13 2014-12-16 Abbvie Inc. Fed-batch method of making human anti-TNF-alpha antibody
AU2007294731B2 (en) 2006-09-13 2014-04-17 Abbvie Inc. Cell culture improvements
NZ592097A (en) 2008-10-20 2013-01-25 Abbott Lab Viral inactivation during purification of il-12 and il-18 antibodies
CA2911256A1 (en) 2008-10-20 2010-12-09 Robert K. Hickman Isolation and purification of antibodies using protein a affinity chromatography
AU2012283858C1 (en) 2011-07-20 2020-01-23 Zepteon, Incorporated Polypeptide separation methods
JP6451119B2 (ja) * 2014-07-17 2019-01-16 東ソー株式会社 抗体依存性細胞傷害活性の強さに基づき抗体を分離する方法
WO2017079637A1 (en) 2015-11-04 2017-05-11 Biogen Ma Inc. Conjugation methods for modifying or immobilizing proteins
SG11202003765RA (en) * 2017-04-06 2020-05-28 Flodesign Sonics Inc Acoustic perfusion devices
CN114096560A (zh) * 2019-07-03 2022-02-25 默克专利股份公司 糖型纯化
JPWO2021010349A1 (es) * 2019-07-12 2021-01-21
GB202204016D0 (en) * 2022-03-22 2022-05-04 Ucl Business Plc Affinity chromatography ligands for antibody glycovariant separation

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1988007089A1 (en) * 1987-03-18 1988-09-22 Medical Research Council Altered antibodies
US6410690B1 (en) * 1995-06-07 2002-06-25 Medarex, Inc. Therapeutic compounds comprised of anti-Fc receptor antibodies
PT1071700E (pt) 1998-04-20 2010-04-23 Glycart Biotechnology Ag Modificação por glicosilação de anticorpos para melhorar a citotoxicidade celular dependente de anticorpos
EP1006183A1 (en) * 1998-12-03 2000-06-07 Max-Planck-Gesellschaft zur Förderung der Wissenschaften e.V. Recombinant soluble Fc receptors
CN1555411A (zh) 2001-08-03 2004-12-15 ���迨�����＼���ɷݹ�˾ 抗体-依赖性细胞毒性增大的抗体糖基化变体
NZ541503A (en) 2003-01-22 2008-09-26 Glycart Biotechnology Ag Fusion constructs and use of same to produce antibodies with increased Fc receptor binding affinity and effector function
RU2412200C2 (ru) * 2004-11-12 2011-02-20 Ксенкор, Инк. Fc-ВАРИАНТЫ С ИЗМЕНЕННЫМ СВЯЗЫВАНИЕМ С FcRn
US7699979B2 (en) * 2005-01-07 2010-04-20 Board Of Trustees Of The University Of Arkansas Separation system and efficient capture of contaminants using magnetic nanoparticles
EP2388274A1 (en) * 2005-06-17 2011-11-23 Janssen Alzheimer Immunotherapy Methods of purifying anti A Beta antibodies
WO2010048313A2 (en) * 2008-10-22 2010-04-29 Biogen Idec Ma Inc. Recombinant fcrn and variants thereof for purification of fc-containing fusion proteins
EP2483693B1 (en) 2009-10-02 2014-12-03 Roche Glycart AG A-fucosylation detection in antibodies
AU2012283858C1 (en) * 2011-07-20 2020-01-23 Zepteon, Incorporated Polypeptide separation methods

Also Published As

Publication number Publication date
US9994610B2 (en) 2018-06-12
RU2650873C2 (ru) 2018-04-17
CN103890002A (zh) 2014-06-25
RU2014118552A (ru) 2015-11-27
JP2014530829A (ja) 2014-11-20
US20180265544A1 (en) 2018-09-20
CN108314729A (zh) 2018-07-24
WO2013057078A1 (en) 2013-04-25
JP2018184406A (ja) 2018-11-22
MX2014004604A (es) 2014-05-27
HK1258766A1 (zh) 2019-11-22
HK1199272A1 (en) 2015-06-26
JP6356605B2 (ja) 2018-07-11
ES2619677T3 (es) 2017-06-26
EP2768845B1 (en) 2017-01-18
CA2851053A1 (en) 2013-04-25
BR112014009178A2 (pt) 2018-09-04
EP2768845A1 (en) 2014-08-27
US20140255399A1 (en) 2014-09-11
KR20140077178A (ko) 2014-06-23

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