BG49049A3 - Method for clonalling and exposure of genetics coding enzyms - Google Patents

Method for clonalling and exposure of genetics coding enzyms

Info

Publication number
BG49049A3
BG49049A3 BG085831A BG8583188A BG49049A3 BG 49049 A3 BG49049 A3 BG 49049A3 BG 085831 A BG085831 A BG 085831A BG 8583188 A BG8583188 A BG 8583188A BG 49049 A3 BG49049 A3 BG 49049A3
Authority
BG
Bulgaria
Prior art keywords
clonalling
enzyms
genetics
coding
exposure
Prior art date
Application number
BG085831A
Other languages
Bulgarian (bg)
English (en)
Inventor
Christiaan A.G. Eekelen
Johannes Cornelis Van Laan
Leonardus Mulleners
Original Assignee
Genencor International Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc. filed Critical Genencor International Inc.
Publication of BG49049A3 publication Critical patent/BG49049A3/xx

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BG085831A 1987-02-27 1988-10-25 Method for clonalling and exposure of genetics coding enzyms BG49049A3 (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
PCT/NL1988/000007 WO1988006624A2 (en) 1987-02-27 1988-02-26 Molecular cloning and expression of genes encoding proteolytic enzymes

Publications (1)

Publication Number Publication Date
BG49049A3 true BG49049A3 (en) 1991-07-15

Family

ID=8197584

Family Applications (1)

Application Number Title Priority Date Filing Date
BG085831A BG49049A3 (en) 1987-02-27 1988-10-25 Method for clonalling and exposure of genetics coding enzyms

Country Status (19)

Country Link
US (1) US5217878A (fi)
JP (1) JP3026567B2 (fi)
KR (1) KR0127560B1 (fi)
CN (1) CN88101681A (fi)
AT (2) ATE181110T1 (fi)
AU (1) AU620026B2 (fi)
BG (1) BG49049A3 (fi)
BR (1) BR8805647A (fi)
CA (1) CA1339100C (fi)
DE (2) DE3856331T2 (fi)
DK (2) DK175738B1 (fi)
FI (3) FI105483B (fi)
HU (1) HU213220B (fi)
LT (1) LT4000B (fi)
LV (1) LV10307B (fi)
NO (1) NO884423D0 (fi)
PT (1) PT86864B (fi)
RU (1) RU2023723C1 (fi)
WO (1) WO1988006624A2 (fi)

Families Citing this family (50)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR0127560B1 (ko) * 1987-02-27 1997-12-29 마가렛트 에이.호른 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현
DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
AU622916B2 (en) * 1988-05-05 1992-04-30 Applied Microbiology, Inc Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products
US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DE69333463D1 (de) * 1992-05-18 2004-05-06 Genencor Int Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
CN1530443A (zh) 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ 枯草杆菌酶变异体和组合物
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6485957B1 (en) * 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6458564B1 (en) * 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
DK2339016T3 (da) 2002-03-29 2017-01-23 Danisco Us Inc Øget produktion af subtilisiner
EP1625217B1 (en) * 2003-05-12 2014-12-17 Danisco US Inc. Novel lipolytic enzyme elip
EP1625202A4 (en) 2003-05-12 2010-10-20 Genencor Int NEW LIPOLYTIC ENZYME LIP1
EP1625208A4 (en) * 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
AU2004277913B2 (en) 2003-09-19 2010-04-08 Bakery Technology Centre Bv Preparation of an edible product from dough
EP1694847B1 (en) * 2003-11-19 2012-06-13 Danisco US Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
EP2129779B2 (en) 2007-03-12 2018-12-26 Danisco US Inc. Modified proteases
US8343735B2 (en) * 2007-05-10 2013-01-01 Danisco Us Inc. Modified secretion system to increase expression of polypeptides in bacteria
ES2562919T3 (es) 2007-08-06 2016-03-09 University Of Chile Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío
RU2515112C2 (ru) 2007-12-21 2014-05-10 ДАНИСКО ЮЭс ИНК. Улучшенная продукция белка в bacillus
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2751266B1 (en) * 2011-09-22 2017-03-29 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
CN103013960A (zh) * 2012-12-21 2013-04-03 青岛蔚蓝生物集团有限公司 一种碱性蛋白酶及其重组表达工程菌
MX2016008595A (es) 2013-12-31 2016-10-28 Danisco Us Inc Expresion mejorada de proteínas.
KR102500121B1 (ko) 2014-12-16 2023-02-20 다니스코 유에스 인크. 향상된 단백질 발현
EP3234149A1 (en) 2014-12-19 2017-10-25 Danisco US Inc. Enhanced protein expression
EP3619309B1 (en) * 2017-05-05 2024-04-10 Bioecho Life Sciences GmbH Rapid purification of high quality nucleic acids from biological samples

Family Cites Families (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (fi) 1967-10-03 1971-06-03
GB1205403A (en) 1967-11-10 1970-09-16 Novo Terapeutisk Labor As Preparation of proteolytic enzyme preparations
GB1519148A (en) * 1974-11-19 1978-07-26 Gist Brocades Nv Compositions of matter
FR2482980A1 (fr) 1980-05-20 1981-11-27 Biomerieux Sa Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues
JPS6055118B2 (ja) 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
DE3380878D1 (en) * 1982-11-01 1989-12-28 Miles Inc Method of heterologous cloning of gene in bacillus microorganism
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
DE3480411D1 (en) * 1983-07-06 1989-12-14 Gist Brocades Nv Molecular cloning and expression in industrial microorganism species
EP0138075A1 (en) * 1983-09-16 1985-04-24 Synergen Associates, Inc. A method for the generation and detection of enzyme variants with enhanced themostability and activity
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
FR2582316B1 (fr) * 1985-05-22 1988-12-02 Centre Nat Rech Scient Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase
DE3527913A1 (de) * 1985-08-03 1987-02-12 Henkel Kgaa Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen
JP2599946B2 (ja) * 1986-01-15 1997-04-16 アムジェン，インコーポレイテツド ズブチリシン類似体
ATE116365T1 (de) * 1986-04-30 1995-01-15 Genencor Int Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte.
KR0127560B1 (ko) * 1987-02-27 1997-12-29 마가렛트 에이.호른 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현
ES2135386T3 (es) * 1987-02-27 1999-11-01 Genencor Int Transformacion de cepas de bacillus alcalofilas.

Also Published As

Publication number Publication date
DK593988D0 (da) 1988-10-26
DE3856331T2 (de) 1999-10-14
ATE181110T1 (de) 1999-06-15
DK200401262A (da) 2004-08-20
FI884904A (fi) 1988-10-24
BR8805647A (pt) 1989-10-31
KR0127560B1 (ko) 1997-12-29
KR890700665A (ko) 1989-04-26
LV10307A (lv) 1994-10-20
PT86864A (pt) 1988-03-01
FI20001152A (fi) 2000-05-15
WO1988006624A2 (en) 1988-09-07
CN88101681A (zh) 1988-11-09
FI925413A (fi) 1992-11-27
FI925413A0 (fi) 1992-11-27
DK593988A (da) 1988-10-26
NO884423L (no) 1988-10-05
DE3856339D1 (de) 1999-07-15
DE3856339T2 (de) 1999-11-04
AU1398088A (en) 1988-09-26
AU620026B2 (en) 1992-02-13
DK175738B1 (da) 2005-02-07
DK175852B1 (da) 2005-04-04
JPH01502876A (ja) 1989-10-05
LV10307B (en) 1995-08-20
WO1988006624A3 (en) 1988-11-17
ATE180016T1 (de) 1999-05-15
HU213220B (en) 1997-03-28
FI119028B (fi) 2008-06-30
US5217878A (en) 1993-06-08
CA1339100C (en) 1997-07-29
JP3026567B2 (ja) 2000-03-27
FI105483B (fi) 2000-08-31
FI106726B (fi) 2001-03-30
LTIP1820A (en) 1995-08-25
NO884423D0 (no) 1988-10-05
LT4000B (en) 1996-06-25
PT86864B (pt) 1992-05-29
RU2023723C1 (ru) 1994-11-30
FI884904A0 (fi) 1988-10-24
DE3856331D1 (de) 1999-06-17

Similar Documents

Publication Publication Date Title
BG49049A3 (en) Method for clonalling and exposure of genetics coding enzyms
BG89942A (bg) Метод за получаване на протеолитични ензими
EP0214435A3 (en) Alkaline protease, method for the production of hybrid vectors and genetically transformed microorganisms
EP0283075A3 (en) Molecular cloning and expression of genes encoding proteolytic enzymes
DK0493398T3 (da) Alkalisk, proteolytisk enzym og fremgangsmåde til fremstilling deraf
DK81193D0 (da) Enzym
HK99293A (en) Bacillus incapable of excreting subtilisin or neutral protease
IE880558L (en) Stable gene amplification in prokaryotic chromosomal dna
HU9302252D0 (en) New oxalate oxidases and their applications
DK0453047T3 (da) Penicillin G acylase, et gen kodende herfor og en fremgangsmåde til fremstilling af dette enzym
EP0309550A4 (en) PROTEOLYTIC ENZYMS.
MX9302202A (es) Nueva proteasa fungica.
IL90947A (en) Method for production of hiv protease in transformed cells, dna and vectors comprising said dna used in said production
DE69022909D1 (de) Enzymatische Oxidation von Ceph C zu Glutaryl-7-Aminocephalosporansäure.
BG92653A (bg) Метод за получаване на мутантни протеази
EP0158981A3 (en) New protease and process for production and use thereof
ES2018734A6 (es) Procedimiento para la obtencion de un enzima proteolitico.
ATE22702T1 (de) Herstellung von glucosedehydrogenase.
DE3586410D1 (de) Verfahren zur herstellung von neutraler protease.