DE3586410D1 - Verfahren zur herstellung von neutraler protease. - Google Patents
Verfahren zur herstellung von neutraler protease.Info
- Publication number
- DE3586410D1 DE3586410D1 DE8585830246T DE3586410T DE3586410D1 DE 3586410 D1 DE3586410 D1 DE 3586410D1 DE 8585830246 T DE8585830246 T DE 8585830246T DE 3586410 T DE3586410 T DE 3586410T DE 3586410 D1 DE3586410 D1 DE 3586410D1
- Authority
- DE
- Germany
- Prior art keywords
- neutral protease
- isolating
- hybrid
- culture medium
- producing neutral
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 108090000145 Bacillolysin Proteins 0.000 title abstract 4
- 102000035092 Neutral proteases Human genes 0.000 title abstract 4
- 108091005507 Neutral proteases Proteins 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 2
- 239000013612 plasmid Substances 0.000 abstract 3
- 239000001963 growth medium Substances 0.000 abstract 2
- 244000063299 Bacillus subtilis Species 0.000 abstract 1
- 235000014469 Bacillus subtilis Nutrition 0.000 abstract 1
- 238000009630 liquid culture Methods 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 108090000623 proteins and genes Proteins 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Medicinal Chemistry (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fish Paste Products (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
IT23190/84A IT1176997B (it) | 1984-10-17 | 1984-10-17 | Procedimento per la produzione di proteasi neutra |
Publications (2)
Publication Number | Publication Date |
---|---|
DE3586410D1 true DE3586410D1 (de) | 1992-09-03 |
DE3586410T2 DE3586410T2 (de) | 1993-03-04 |
Family
ID=11204709
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE8585830246T Expired - Fee Related DE3586410T2 (de) | 1984-10-17 | 1985-09-30 | Verfahren zur herstellung von neutraler protease. |
Country Status (6)
Country | Link |
---|---|
EP (1) | EP0179025B1 (de) |
JP (1) | JPH0829083B2 (de) |
AT (1) | ATE78870T1 (de) |
CA (1) | CA1285233C (de) |
DE (1) | DE3586410T2 (de) |
IT (1) | IT1176997B (de) |
Families Citing this family (5)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
EP0149241B1 (de) * | 1983-12-28 | 1991-03-27 | Agency Of Industrial Science And Technology | DNS-Basensequenz enthaltende Regionen einbegriffen in der Herstellung und Sekretion eines Proteins, rekombinante DNS das Ganze oder einen Teil der DNS-Basensequenz enthaltend und Verfahren zur Herstellung von Proteinen unter Verwendung der rekombinanten DNS |
IT1186750B (it) * | 1985-07-10 | 1987-12-16 | Eniricerche Spa | Vettore di clonaggio,molecole di dna ricombinante,ceppi di bacillus subtilis trasformati con dette molecole e metodi per l'espressione di geni eterologhi e produzione e secrezione di proteine codificate da detti geni |
IT1239733B (it) * | 1990-02-23 | 1993-11-15 | Eniricerche Spa | Mutanti della proteasi neutra termostabili e mezzi e metodi per la loro preparazione |
CN103859169B (zh) * | 2013-12-13 | 2015-08-12 | 湖南鸿鹰生物科技有限公司 | 一种含中性蛋白酶的饲料复合酶及其制备方法 |
CN103865701B (zh) * | 2013-12-13 | 2016-04-06 | 湖南鸿鹰生物科技有限公司 | 一种含中性蛋白酶的啤酒复合酶 |
Family Cites Families (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPS5867181A (ja) * | 1981-10-16 | 1983-04-21 | Yakult Honsha Co Ltd | スタフイロキナーゼ産生遺伝情報を担うdna断片組み換え体dnaおよびそれを導入した大腸菌 |
EP0151760A1 (de) * | 1984-01-06 | 1985-08-21 | Takeda Chemical Industries, Ltd. | DNA und ihre Verwendung |
-
1984
- 1984-10-17 IT IT23190/84A patent/IT1176997B/it active
-
1985
- 1985-09-30 AT AT85830246T patent/ATE78870T1/de not_active IP Right Cessation
- 1985-09-30 DE DE8585830246T patent/DE3586410T2/de not_active Expired - Fee Related
- 1985-09-30 EP EP85830246A patent/EP0179025B1/de not_active Expired - Lifetime
- 1985-10-02 CA CA000492088A patent/CA1285233C/en not_active Expired - Lifetime
- 1985-10-16 JP JP60230887A patent/JPH0829083B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JPH0829083B2 (ja) | 1996-03-27 |
IT8423190A0 (it) | 1984-10-17 |
EP0179025A1 (de) | 1986-04-23 |
IT8423190A1 (it) | 1986-04-17 |
DE3586410T2 (de) | 1993-03-04 |
ATE78870T1 (de) | 1992-08-15 |
CA1285233C (en) | 1991-06-25 |
JPS61181374A (ja) | 1986-08-14 |
EP0179025B1 (de) | 1992-07-29 |
IT1176997B (it) | 1987-08-26 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8339 | Ceased/non-payment of the annual fee |