BG92653A - Метод за получаване на мутантни протеази - Google Patents

Метод за получаване на мутантни протеази

Info

Publication number
BG92653A
BG92653A BG092653A BG9265390A BG92653A BG 92653 A BG92653 A BG 92653A BG 092653 A BG092653 A BG 092653A BG 9265390 A BG9265390 A BG 9265390A BG 92653 A BG92653 A BG 92653A
Authority
BG
Bulgaria
Prior art keywords
vasillus
strains
mutant proteases
mutant
obtaining mutant
Prior art date
Application number
BG092653A
Other languages
English (en)
Other versions
BG60567B1 (bg
Inventor
Johannes Cornelis Van Iaan
Christiaan Albertus Eekelen
Original Assignee
Genencor International Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=8202457&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BG92653(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Genencor International Inc. filed Critical Genencor International Inc.
Publication of BG92653A publication Critical patent/BG92653A/bg
Publication of BG60567B1 publication Critical patent/BG60567B1/bg

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C12N1/205—Bacterial isolates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Detergent Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Mushroom Cultivation (AREA)

Abstract

Методът намира приложение при производството на детергентни състави. По метода се получават алкалофилни щамове васillus, които продуцират мутантна протеаза, отличаваща се най-малко с една аминокиселина от дивия тип алкална протеаза. Щамовете васillus се трансформират с плазмиден експресионен вектор, съдържащ мутантен ген на алкалната протеаза. 21 претенции, 15 фигури
BG92653A 1989-08-11 1990-08-08 Метод за получаване на мутантни протеази BG60567B1 (bg)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP89202117 1989-08-11

Publications (2)

Publication Number Publication Date
BG92653A true BG92653A (bg) 1993-12-24
BG60567B1 BG60567B1 (bg) 1995-08-28

Family

ID=8202457

Family Applications (1)

Application Number Title Priority Date Filing Date
BG92653A BG60567B1 (bg) 1989-08-11 1990-08-08 Метод за получаване на мутантни протеази

Country Status (19)

Country Link
US (1) US20040014175A1 (bg)
EP (1) EP0414297B1 (bg)
JP (1) JP3490435B2 (bg)
KR (1) KR0169978B1 (bg)
CN (1) CN1049866A (bg)
AT (1) ATE144283T1 (bg)
AU (1) AU629970B2 (bg)
BG (1) BG60567B1 (bg)
BR (1) BR9003956A (bg)
CA (1) CA2023094C (bg)
DD (1) DD297187A5 (bg)
DE (1) DE69028890T2 (bg)
DK (1) DK0414297T3 (bg)
ES (1) ES2095233T3 (bg)
FI (1) FI110365B (bg)
GR (1) GR3021669T3 (bg)
IE (1) IE77037B1 (bg)
PT (1) PT94961B (bg)
RU (1) RU2060276C1 (bg)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4304161A1 (de) * 1993-02-12 1994-08-25 Solvay Enzymes Gmbh & Co Kg Verbesserte hochalkalische Proteasen
DK0772684T3 (da) 1994-06-17 2005-12-12 Genencor Int Amylolytiske enzymer afledt fra B. Licheniformis alpha-amylasen med forbedrede karakteristika
DK0894126T3 (da) * 1996-03-27 2006-06-12 Novozymes As Alkalisk phosphatase-deficient trådformet svamp
JP3440694B2 (ja) * 1996-05-16 2003-08-25 ライオン株式会社 口腔用組成物
GB0024554D0 (en) 2000-10-06 2000-11-22 Agrol Ltd Ethanol production
US8124399B2 (en) * 2002-03-29 2012-02-28 Danisco Us Inc. Enhanced protein expression in Bacillus
WO2006088972A2 (en) 2005-02-16 2006-08-24 The General Hospital Corporation Use of modulatirs of compounds of tgf-beta superfamily to regulate hepcidin-mediated iron metabolism
RU2511416C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) федеральный университет" (ФГАО ВПО КФУ) Штамм бактерий bacillus pumilus 2a-5 с низкой протеолитической активностью, повышенной активностью фосфатазы, способ его получения и применения
RU2510821C1 (ru) * 2012-10-24 2014-04-10 Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) Федеральный Университет" (ФГАОУ ВПО КФУ) Штамм бактерий bacillus pumilus мк-10 с низкой протеолитической активностью и его применение

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB1234445A (bg) * 1967-10-03 1971-06-03
JPS6055118B2 (ja) * 1982-02-08 1985-12-03 昭和電工株式会社 新規な細菌アルカリプロテア−ゼ及びその製造方法
NZ208612A (en) * 1983-06-24 1991-09-25 Genentech Inc Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
AU620326B2 (en) * 1987-02-27 1992-02-20 Dsm Ip Assets B.V. Stable gene amplification in chromosomal dna of prokaryotic microorganisms
EP0479396B1 (en) * 1987-02-27 1999-06-09 Genencor International, Inc. Transformation of alkalophilic bacillus strains
CN1056187C (zh) * 1988-02-11 2000-09-06 金克克国际有限公司 新的蛋白水解酶及其在洗涤剂中的应用

Also Published As

Publication number Publication date
US20040014175A1 (en) 2004-01-22
CA2023094A1 (en) 1991-02-12
CA2023094C (en) 2000-03-28
JPH03210177A (ja) 1991-09-13
PT94961A (pt) 1991-04-18
RU2060276C1 (ru) 1996-05-20
DD297187A5 (de) 1992-01-02
IE77037B1 (en) 1997-11-19
FI110365B (fi) 2002-12-31
KR0169978B1 (ko) 1999-02-01
IE902904A1 (en) 1991-02-27
EP0414297B1 (en) 1996-10-16
BG60567B1 (bg) 1995-08-28
PT94961B (pt) 1997-05-28
GR3021669T3 (en) 1997-02-28
CN1049866A (zh) 1991-03-13
EP0414297A1 (en) 1991-02-27
ES2095233T3 (es) 1997-02-16
JP3490435B2 (ja) 2004-01-26
KR910004805A (ko) 1991-03-29
AU629970B2 (en) 1992-10-15
FI903927A0 (fi) 1990-08-08
DK0414297T3 (da) 1997-07-07
DE69028890T2 (de) 1997-02-27
DE69028890D1 (de) 1996-11-21
AU6096190A (en) 1991-02-14
BR9003956A (pt) 1991-09-03
ATE144283T1 (de) 1996-11-15

Similar Documents

Publication Publication Date Title
BG89942A (bg) Метод за получаване на протеолитични ензими
HK75493A (en) Procaryotic carbonyl hydrolases,methods,dna,vectors and transformed hosts for producing them,and detergent compositions containing them
ES2164644T3 (es) Enzimas proteoliticas mutantes de bacillus.
DK390784D0 (da) Enzymatisk rengoeringsmiddel til maskinopvask
MY107664A (en) Novel alkaline proteinase and process for producing the same
EP0415296A3 (en) High alkaline proteases
BG92653A (bg) Метод за получаване на мутантни протеази
GB1395895A (en) Enzyme products
EP0309550A4 (en) PROTEOLYTIC ENZYMS.
DE3578516D1 (de) Haarwaschmittelzusammensetzung.
EP0319460A3 (en) Cleaning composition containing protease produced by vibrio
IE890544L (en) New lipase and detergent compositions containing it
FI931634A0 (fi) Nytt svampproteas
ATE207534T1 (de) Neuartige proteasen
ES2073174T3 (es) Nuevas proteasas.
ES2018734A6 (es) Procedimiento para la obtencion de un enzima proteolitico.
DK0578712T3 (da) Hidtil ukendte proteaser
JPS6474986A (en) Protease and a novel microorganism producing the same
ES8605574A1 (es) Procedimiento para preparar una alcoholdeshidrogenasa
MX9705968A (es) Proteasas de bacilos.