BG92653A - Метод за получаване на мутантни протеази - Google Patents
Метод за получаване на мутантни протеазиInfo
- Publication number
- BG92653A BG92653A BG092653A BG9265390A BG92653A BG 92653 A BG92653 A BG 92653A BG 092653 A BG092653 A BG 092653A BG 9265390 A BG9265390 A BG 9265390A BG 92653 A BG92653 A BG 92653A
- Authority
- BG
- Bulgaria
- Prior art keywords
- vasillus
- strains
- mutant proteases
- mutant
- obtaining mutant
- Prior art date
Links
- 108091005804 Peptidases Proteins 0.000 title abstract 2
- 239000004365 Protease Substances 0.000 title abstract 2
- 102000035195 Peptidases Human genes 0.000 title 1
- 108091005658 Basic proteases Proteins 0.000 abstract 2
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 abstract 1
- 150000001413 amino acids Chemical class 0.000 abstract 1
- 239000003599 detergent Substances 0.000 abstract 1
- 239000013604 expression vector Substances 0.000 abstract 1
- 239000000203 mixture Substances 0.000 abstract 1
- 239000013612 plasmid Substances 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/07—Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Detergent Compositions (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Compounds Of Unknown Constitution (AREA)
- Mushroom Cultivation (AREA)
Abstract
Методът намира приложение при производството на детергентни състави. По метода се получават алкалофилни щамове васillus, които продуцират мутантна протеаза, отличаваща се най-малко с една аминокиселина от дивия тип алкална протеаза. Щамовете васillus се трансформират с плазмиден експресионен вектор, съдържащ мутантен ген на алкалната протеаза. 21 претенции, 15 фигури
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP89202117 | 1989-08-11 |
Publications (2)
Publication Number | Publication Date |
---|---|
BG92653A true BG92653A (bg) | 1993-12-24 |
BG60567B1 BG60567B1 (bg) | 1995-08-28 |
Family
ID=8202457
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
BG92653A BG60567B1 (bg) | 1989-08-11 | 1990-08-08 | Метод за получаване на мутантни протеази |
Country Status (19)
Country | Link |
---|---|
US (1) | US20040014175A1 (bg) |
EP (1) | EP0414297B1 (bg) |
JP (1) | JP3490435B2 (bg) |
KR (1) | KR0169978B1 (bg) |
CN (1) | CN1049866A (bg) |
AT (1) | ATE144283T1 (bg) |
AU (1) | AU629970B2 (bg) |
BG (1) | BG60567B1 (bg) |
BR (1) | BR9003956A (bg) |
CA (1) | CA2023094C (bg) |
DD (1) | DD297187A5 (bg) |
DE (1) | DE69028890T2 (bg) |
DK (1) | DK0414297T3 (bg) |
ES (1) | ES2095233T3 (bg) |
FI (1) | FI110365B (bg) |
GR (1) | GR3021669T3 (bg) |
IE (1) | IE77037B1 (bg) |
PT (1) | PT94961B (bg) |
RU (1) | RU2060276C1 (bg) |
Families Citing this family (9)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE4304161A1 (de) * | 1993-02-12 | 1994-08-25 | Solvay Enzymes Gmbh & Co Kg | Verbesserte hochalkalische Proteasen |
DK0772684T3 (da) | 1994-06-17 | 2005-12-12 | Genencor Int | Amylolytiske enzymer afledt fra B. Licheniformis alpha-amylasen med forbedrede karakteristika |
DK0894126T3 (da) * | 1996-03-27 | 2006-06-12 | Novozymes As | Alkalisk phosphatase-deficient trådformet svamp |
JP3440694B2 (ja) * | 1996-05-16 | 2003-08-25 | ライオン株式会社 | 口腔用組成物 |
GB0024554D0 (en) | 2000-10-06 | 2000-11-22 | Agrol Ltd | Ethanol production |
US8124399B2 (en) * | 2002-03-29 | 2012-02-28 | Danisco Us Inc. | Enhanced protein expression in Bacillus |
WO2006088972A2 (en) | 2005-02-16 | 2006-08-24 | The General Hospital Corporation | Use of modulatirs of compounds of tgf-beta superfamily to regulate hepcidin-mediated iron metabolism |
RU2511416C1 (ru) * | 2012-10-24 | 2014-04-10 | Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) федеральный университет" (ФГАО ВПО КФУ) | Штамм бактерий bacillus pumilus 2a-5 с низкой протеолитической активностью, повышенной активностью фосфатазы, способ его получения и применения |
RU2510821C1 (ru) * | 2012-10-24 | 2014-04-10 | Федеральное государственное автономное образовательное учреждение высшего профессионального образования "Казанский (Приволжский) Федеральный Университет" (ФГАОУ ВПО КФУ) | Штамм бактерий bacillus pumilus мк-10 с низкой протеолитической активностью и его применение |
Family Cites Families (7)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1234445A (bg) * | 1967-10-03 | 1971-06-03 | ||
JPS6055118B2 (ja) * | 1982-02-08 | 1985-12-03 | 昭和電工株式会社 | 新規な細菌アルカリプロテア−ゼ及びその製造方法 |
NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
AU620326B2 (en) * | 1987-02-27 | 1992-02-20 | Dsm Ip Assets B.V. | Stable gene amplification in chromosomal dna of prokaryotic microorganisms |
EP0479396B1 (en) * | 1987-02-27 | 1999-06-09 | Genencor International, Inc. | Transformation of alkalophilic bacillus strains |
CN1056187C (zh) * | 1988-02-11 | 2000-09-06 | 金克克国际有限公司 | 新的蛋白水解酶及其在洗涤剂中的应用 |
-
1990
- 1990-08-02 DE DE69028890T patent/DE69028890T2/de not_active Expired - Lifetime
- 1990-08-02 EP EP90202109A patent/EP0414297B1/en not_active Expired - Lifetime
- 1990-08-02 AT AT90202109T patent/ATE144283T1/de not_active IP Right Cessation
- 1990-08-02 DK DK90202109.6T patent/DK0414297T3/da active
- 1990-08-02 ES ES90202109T patent/ES2095233T3/es not_active Expired - Lifetime
- 1990-08-08 DD DD90343328A patent/DD297187A5/de not_active IP Right Cessation
- 1990-08-08 BG BG92653A patent/BG60567B1/bg unknown
- 1990-08-08 FI FI903927A patent/FI110365B/fi active IP Right Grant
- 1990-08-10 CA CA002023094A patent/CA2023094C/en not_active Expired - Lifetime
- 1990-08-10 PT PT94961A patent/PT94961B/pt not_active IP Right Cessation
- 1990-08-10 BR BR909003956A patent/BR9003956A/pt not_active Application Discontinuation
- 1990-08-10 IE IE290490A patent/IE77037B1/en not_active IP Right Cessation
- 1990-08-10 JP JP21356290A patent/JP3490435B2/ja not_active Expired - Lifetime
- 1990-08-10 RU SU904830790A patent/RU2060276C1/ru active
- 1990-08-10 AU AU60961/90A patent/AU629970B2/en not_active Expired
- 1990-08-11 CN CN90107928A patent/CN1049866A/zh active Pending
- 1990-08-11 KR KR1019900012377A patent/KR0169978B1/ko not_active IP Right Cessation
-
1996
- 1996-11-14 GR GR960403043T patent/GR3021669T3/el unknown
-
2003
- 2003-05-19 US US10/442,013 patent/US20040014175A1/en not_active Abandoned
Also Published As
Publication number | Publication date |
---|---|
US20040014175A1 (en) | 2004-01-22 |
CA2023094A1 (en) | 1991-02-12 |
CA2023094C (en) | 2000-03-28 |
JPH03210177A (ja) | 1991-09-13 |
PT94961A (pt) | 1991-04-18 |
RU2060276C1 (ru) | 1996-05-20 |
DD297187A5 (de) | 1992-01-02 |
IE77037B1 (en) | 1997-11-19 |
FI110365B (fi) | 2002-12-31 |
KR0169978B1 (ko) | 1999-02-01 |
IE902904A1 (en) | 1991-02-27 |
EP0414297B1 (en) | 1996-10-16 |
BG60567B1 (bg) | 1995-08-28 |
PT94961B (pt) | 1997-05-28 |
GR3021669T3 (en) | 1997-02-28 |
CN1049866A (zh) | 1991-03-13 |
EP0414297A1 (en) | 1991-02-27 |
ES2095233T3 (es) | 1997-02-16 |
JP3490435B2 (ja) | 2004-01-26 |
KR910004805A (ko) | 1991-03-29 |
AU629970B2 (en) | 1992-10-15 |
FI903927A0 (fi) | 1990-08-08 |
DK0414297T3 (da) | 1997-07-07 |
DE69028890T2 (de) | 1997-02-27 |
DE69028890D1 (de) | 1996-11-21 |
AU6096190A (en) | 1991-02-14 |
BR9003956A (pt) | 1991-09-03 |
ATE144283T1 (de) | 1996-11-15 |
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