AR076941A1 - Cepa de bacillus para una mayor produccion de proteina - Google Patents

Cepa de bacillus para una mayor produccion de proteina

Info

Publication number
AR076941A1
AR076941A1 ARP100101924A ARP100101924A AR076941A1 AR 076941 A1 AR076941 A1 AR 076941A1 AR P100101924 A ARP100101924 A AR P100101924A AR P100101924 A ARP100101924 A AR P100101924A AR 076941 A1 AR076941 A1 AR 076941A1
Authority
AR
Argentina
Prior art keywords
bacillus
host cells
cepa
protein production
interest
Prior art date
Application number
ARP100101924A
Other languages
English (en)
Original Assignee
Danisco Us Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Danisco Us Inc filed Critical Danisco Us Inc
Publication of AR076941A1 publication Critical patent/AR076941A1/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Células huésped que han sido manipuladas genéticamente para tener una mejor capacidad para producir proteínas de interés. En particular, se refiere a células huésped de Bacillus sp. que tienen al menos un gen de phr inactivado. El aumento de produccion de las proteínas de interés por las células huésped de Bacillus sp. modificadas está adicionalmente aumentado en las células huésped de Bacillus sp. modificadas que sobreexpresan YmaH. También se proporcionan métodos para producir proteínas de interés en las células huésped modificadas.
ARP100101924A 2009-06-11 2010-06-01 Cepa de bacillus para una mayor produccion de proteina AR076941A1 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US18632109P 2009-06-11 2009-06-11

Publications (1)

Publication Number Publication Date
AR076941A1 true AR076941A1 (es) 2011-07-20

Family

ID=42562872

Family Applications (1)

Application Number Title Priority Date Filing Date
ARP100101924A AR076941A1 (es) 2009-06-11 2010-06-01 Cepa de bacillus para una mayor produccion de proteina

Country Status (6)

Country Link
US (2) US8293499B2 (es)
EP (1) EP2440573B1 (es)
CN (1) CN102803290B (es)
AR (1) AR076941A1 (es)
DK (1) DK2440573T3 (es)
WO (1) WO2010144283A1 (es)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN104053780A (zh) * 2011-12-09 2014-09-17 丹尼斯科美国公司 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子
MX2016008595A (es) 2013-12-31 2016-10-28 Danisco Us Inc Expresion mejorada de proteínas.
KR101619316B1 (ko) 2014-05-27 2016-05-11 한국생명공학연구원 바실러스 프로모터 변이체 및 이를 이용한 단백질의 제조방법
DE102014223904A1 (de) * 2014-11-24 2016-05-25 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Kondensator und Verfahren zum Herstellen desselben
WO2016099917A1 (en) 2014-12-16 2016-06-23 Danisco Us Inc Enhanced protein expression
EP3234149A1 (en) * 2014-12-19 2017-10-25 Danisco US Inc. Enhanced protein expression
CN107250365A (zh) * 2015-02-19 2017-10-13 丹尼斯科美国公司 增强的蛋白质表达
US11306319B2 (en) 2015-10-30 2022-04-19 Danisco Us Inc. Enhanced protein expression and methods thereof
US20180371445A1 (en) * 2015-12-23 2018-12-27 Danisco Us Inc. Enhanced protein production and methods thereof
EP3419991B1 (en) 2016-03-04 2022-10-26 Danisco US Inc. Engineered ribosomal promoters for protein production in microorganisms
CN108456669B (zh) * 2017-02-20 2023-08-08 上海凯赛生物技术股份有限公司 一种核糖体结合位点、重组表达质粒、转化子及其应用
JP7280269B2 (ja) * 2018-01-03 2023-05-23 ダニスコ・ユーエス・インク タンパク質の産生増加のための変異及び遺伝子改変バチルス属（ｂａｃｉｌｌｕｓ）細胞、並びにその方法
CN109355303B (zh) * 2018-11-14 2022-05-06 天津大学 抑制和/或敲除基因在提高单克隆抗体的表达量中的应用
JP2022509215A (ja) * 2018-11-28 2022-01-20 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞におけるタンパク質産生増強のための新規なプロモーター配列及びその方法
WO2020169564A1 (en) * 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
CN111004815B (zh) * 2019-12-18 2023-07-21 江苏省农业科学院 通过突变degU基因提高枯草芽孢杆菌表面活性素产量的方法
CN112646746B (zh) * 2021-01-04 2022-02-25 济南大学 一株高效降解驴毛的坚强芽孢杆菌
CN112941088B (zh) * 2021-02-04 2023-06-16 中国农业科学院哈尔滨兽医研究所（中国动物卫生与流行病学中心哈尔滨分中心） 与布氏杆菌毒力相关的基因及其在布氏杆菌毒力评价及制备弱毒布氏杆菌中的应用
CN115260314B (zh) * 2022-05-10 2023-10-24 吉林大学 一种重组嗜热中性蛋白酶及其在降解蛋白质中的应用

Family Cites Families (37)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4302544A (en) 1979-10-15 1981-11-24 University Of Rochester Asporogenous mutant of B. subtilis for use as host component of HV1 system
US4450235A (en) 1982-04-21 1984-05-22 Cpc International Inc. Asporogenic mutant of bacillus subtilis useful as a host in a host-vector system
US5310675A (en) 1983-06-24 1994-05-10 Genencor, Inc. Procaryotic carbonyl hydrolases
US4760025A (en) 1984-05-29 1988-07-26 Genencor, Inc. Modified enzymes and methods for making same
NZ208806A (en) 1983-07-06 1988-07-28 Gist Brocades Nv Genetic engineering of industrial microorganism species: readily-transformable host used as intermediate in transfer of dna to the industrial species; plasmids
US5264366A (en) 1984-05-29 1993-11-23 Genencor, Inc. Protease deficient bacillus
US5700676A (en) 1984-05-29 1997-12-23 Genencor International Inc. Modified subtilisins having amino acid alterations
WO1987004461A1 (en) 1986-01-15 1987-07-30 Amgen THERMALLY STABLE AND pH STABLE SUBTILISIN ANALOGS AND METHOD FOR PRODUCTION THEREOF
US4980288A (en) 1986-02-12 1990-12-25 Genex Corporation Subtilisin with increased thermal stability
GB8608194D0 (en) 1986-04-03 1986-05-08 Massey Ferguson Services Nv Valve control system
US4873192A (en) 1987-02-17 1989-10-10 The United States Of America As Represented By The Department Of Health And Human Services Process for site specific mutagenesis without phenotypic selection
HU213220B (en) 1987-02-27 1997-03-28 Gist Brocades Nv Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells
US4914031A (en) 1987-04-10 1990-04-03 Amgen, Inc. Subtilisin analogs
DK6488D0 (da) 1988-01-07 1988-01-07 Novo Industri As Enzymer
PT89702B (pt) 1988-02-11 1994-04-29 Gist Brocades Nv Processo para a preparacao de novos enzimas proteoliticos e de detergentes que os contem
US5665587A (en) 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
DK97190D0 (da) 1990-04-19 1990-04-19 Novo Nordisk As Oxidationsstabile detergentenzymer
US5482849A (en) 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DE69230825T2 (de) 1991-04-26 2000-11-30 Genencor Int Genexpression in bazillen
EP0583339B1 (en) 1991-05-01 1998-07-08 Novo Nordisk A/S Stabilized enzymes and detergent compositions
US5698415A (en) 1991-11-13 1997-12-16 Novo Nordisk Als Bacillus promoter derived from a variant of a bacillus licheniformis X-amylase promoter
DE4411223A1 (de) 1994-03-31 1995-10-05 Solvay Enzymes Gmbh & Co Kg Verwendung alkalischer Proteasen in gewerblichen Textilwaschverfahren
CN1243827C (zh) 1996-08-23 2006-03-01 彼得·鲁戴尔·简森 选定生物体的人工启动子文库和得自这种文库的启动子
AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
EP1042501B2 (en) 1997-12-24 2011-03-30 Genencor International, Inc. Method for assaying the wash performance of an enzyme.
US5955310A (en) 1998-02-26 1999-09-21 Novo Nordisk Biotech, Inc. Methods for producing a polypeptide in a bacillus cell
US6835550B1 (en) 1998-04-15 2004-12-28 Genencor International, Inc. Mutant proteins having lower allergenic response in humans and methods for constructing, identifying and producing such proteins
US6376450B1 (en) 1998-10-23 2002-04-23 Chanchal Kumar Ghosh Cleaning compositions containing multiply-substituted protease variants
US6509185B1 (en) 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP1309677B2 (en) 2000-08-11 2012-04-11 Genencor International, Inc. Bacillus transformation, transformants and mutant libraries
ATE495245T1 (de) * 2002-02-15 2011-01-15 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
ATE530560T1 (de) 2003-11-06 2011-11-15 Danisco Us Inc Vegf bindende und geträgerte peptide zur behandlung von hautkrankheiten
CA2562208A1 (en) * 2004-04-09 2006-03-30 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
JP4832153B2 (ja) * 2005-04-28 2011-12-07 花王株式会社 組換え微生物
JP5140307B2 (ja) * 2007-04-10 2013-02-06 花王株式会社 組換え微生物
JP2009072154A (ja) * 2007-09-21 2009-04-09 Kao Corp 新規枯草菌変異株及び長寿命化した枯草菌変異株の作製方法
AU2008348270A1 (en) * 2007-12-21 2009-07-30 Danisco Us Inc. Enhanced protein production in bacillus

Also Published As

Publication number Publication date
CN102803290A (zh) 2012-11-28
US20120142076A1 (en) 2012-06-07
DK2440573T3 (en) 2015-01-19
US20130029402A1 (en) 2013-01-31
EP2440573A1 (en) 2012-04-18
CN102803290B (zh) 2015-11-25
WO2010144283A1 (en) 2010-12-16
US8293499B2 (en) 2012-10-23
EP2440573B1 (en) 2014-10-15
US8476042B2 (en) 2013-07-02

Similar Documents

Publication Publication Date Title
AR076941A1 (es) Cepa de bacillus para una mayor produccion de proteina
CY1122268T1 (el) Πρωτεϊνες προσδεσης καλλικρεϊνης πλασματος
EA201270019A1 (ru) Двуцепочечная рнк, включенная в липидный состав и мишенью которой является ген pcsk9
BR112013010551A2 (pt) variantes de isopreno sintase para a produção aprimorada de isopreno
CO6440530A2 (es) Formulaciones de caldo de fermentación
BR112013027307A2 (pt) método para tratar uma matriz de tecido; matriz de tecido acelular
BR112014001834A2 (pt) biocontrole de nematoides
BR112016005286A2 (pt) composições e métodos compreendendo variantes de protease do clado lg12
UY34333A (es) ?métodos y composiciones para el control de malezas, y métodos para reducir la expresión de enzima dhps?
CL2013001124A1 (es) Anticuerpo aislado o una proteína que comprende una porción de enlace de antígeno de un anticuerpo dirigido contra un polipéptido de cd40; composicion farmacéutica que comprende dicho anticuerpo; ácido nucleico que codifica el anticuerpo; vector de clonación; proceso de producción del anticuerpo.
AR068559A1 (es) Proteinas de dedo de zinc obtenidas por ingenieria dirigidas a genes de 5-enolpiruvilshikimato-3-fosfatosintetasa
AR080009A1 (es) Alteracion genomica objetivo utilizando nucleasas de dedos de zinc
BR112013020767A2 (pt) composições compreendendo ácido peróxi alfa-cetocarboxílico e métodos para a produção e o uso do mesmo
EA201101521A1 (ru) Способы лечения онкологических заболеваний, использующие эпиметаболические переключатели, многоаспектные внутриклеточные молекулы или факторы влияния
BR112012017345A2 (pt) "composições e métodos de pgl para a produção aumentada de isopreno e outros produtos com 6-fosfogliconolactonase (pgl)"
CY1118856T1 (el) Εμβολιο pcsk9
AR075029A1 (es) Metodo para producir una proteina o un peptido fisiologicamente activo usando un fragmento de inmunoglobulina
UY34558A (es) Proteínas de unión específicas duales dirigidas contra il-13 y/o il-17
BR112014027488A2 (pt) isopreno sintase de legumes para produção de isopreno
PE20150603A1 (es) Metodo para producir iduronato-2-sulfatasa recombinante
PE20220960A1 (es) Proteinas de variantes de secuencias de aminoacidos de cry1da1 activas para lepidopteros
AR076311A1 (es) Proteasas con regiones pre- pro modificadas
MY182946A (en) Production of organic acids by fermentation at low ph
IN2014DN10710A (es)
GB2497453A (en) Pharmaceutical composition and methods of treating and preventing the diseases caused by HIV or associated with HIV

Legal Events

Date Code Title Description
FB Suspension of granting procedure