NO308543B1 - FremgangsmÕte for fremstilling av hirudinderivater, samt rekombinant DNA og rekombinant vektor - Google Patents
FremgangsmÕte for fremstilling av hirudinderivater, samt rekombinant DNA og rekombinant vektor Download PDFInfo
- Publication number
- NO308543B1 NO308543B1 NO911137A NO911137A NO308543B1 NO 308543 B1 NO308543 B1 NO 308543B1 NO 911137 A NO911137 A NO 911137A NO 911137 A NO911137 A NO 911137A NO 308543 B1 NO308543 B1 NO 308543B1
- Authority
- NO
- Norway
- Prior art keywords
- hirudin
- recombinant vector
- signal peptide
- thr
- coli
- Prior art date
Links
- WQPDUTSPKFMPDP-OUMQNGNKSA-N hirudin Chemical class C([C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H]([C@@H](C)CC)C(=O)N1[C@@H](CCC1)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CCC(O)=O)C(=O)N[C@@H](CC=1C=CC(OS(O)(=O)=O)=CC=1)C(=O)N[C@@H](CC(C)C)C(=O)N[C@@H](CCC(N)=O)C(O)=O)NC(=O)[C@H](CC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CC=1NC=NC=1)NC(=O)[C@H](CO)NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H]1N(CCC1)C(=O)[C@H](CCCCN)NC(=O)[C@H]1N(CCC1)C(=O)[C@@H](NC(=O)CNC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@@H](NC(=O)[C@@H](NC(=O)[C@H]1NC(=O)[C@H](CCC(N)=O)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCCCN)NC(=O)[C@H](CCC(O)=O)NC(=O)CNC(=O)[C@H](CC(O)=O)NC(=O)[C@H](CO)NC(=O)CNC(=O)[C@H](CC(C)C)NC(=O)[C@H]([C@@H](C)CC)NC(=O)[C@@H]2CSSC[C@@H](C(=O)N[C@@H](CCC(O)=O)C(=O)NCC(=O)N[C@@H](CO)C(=O)N[C@@H](CC(N)=O)C(=O)N[C@H](C(=O)N[C@H](C(NCC(=O)N[C@@H](CCC(N)=O)C(=O)NCC(=O)N[C@@H](CC(N)=O)C(=O)N[C@@H](CCCCN)C(=O)N2)=O)CSSC1)C(C)C)NC(=O)[C@H](CC(C)C)NC(=O)[C@H]1NC(=O)[C@H](CC(C)C)NC(=O)[C@H](CC(N)=O)NC(=O)[C@H](CCC(N)=O)NC(=O)CNC(=O)[C@H](CO)NC(=O)[C@H](CCC(O)=O)NC(=O)[C@H]([C@@H](C)O)NC(=O)[C@@H](NC(=O)[C@H](CC(O)=O)NC(=O)[C@@H](NC(=O)[C@H](CC=2C=CC(O)=CC=2)NC(=O)[C@@H](NC(=O)[C@@H](N)C(C)C)C(C)C)[C@@H](C)O)CSSC1)C(C)C)[C@@H](C)O)[C@@H](C)O)C1=CC=CC=C1 WQPDUTSPKFMPDP-OUMQNGNKSA-N 0.000 title claims abstract description 64
- 239000013598 vector Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 18
- 108020004511 Recombinant DNA Proteins 0.000 title claims description 3
- 108090000623 proteins and genes Proteins 0.000 claims abstract description 37
- 241000588724 Escherichia coli Species 0.000 claims abstract description 28
- 108010076504 Protein Sorting Signals Proteins 0.000 claims abstract description 21
- 102000004169 proteins and genes Human genes 0.000 claims abstract description 17
- 230000001580 bacterial effect Effects 0.000 claims abstract description 9
- 239000013612 plasmid Substances 0.000 claims description 25
- 230000028327 secretion Effects 0.000 claims description 18
- 238000004519 manufacturing process Methods 0.000 claims description 11
- 239000002609 medium Substances 0.000 claims description 11
- 108020004414 DNA Proteins 0.000 claims description 7
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- 230000001747 exhibiting effect Effects 0.000 claims 1
- 125000001429 N-terminal alpha-amino-acid group Chemical group 0.000 abstract description 3
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- BPHPUYQFMNQIOC-NXRLNHOXSA-N isopropyl beta-D-thiogalactopyranoside Chemical compound CC(C)S[C@@H]1O[C@H](CO)[C@H](O)[C@H](O)[C@H]1O BPHPUYQFMNQIOC-NXRLNHOXSA-N 0.000 description 6
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- 101100506776 Neurospora crassa (strain ATCC 24698 / 74-OR23-1A / CBS 708.71 / DSM 1257 / FGSC 987) hir-1 gene Proteins 0.000 description 3
- YXFVVABEGXRONW-UHFFFAOYSA-N Toluene Chemical compound CC1=CC=CC=C1 YXFVVABEGXRONW-UHFFFAOYSA-N 0.000 description 3
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- AVKUERGKIZMTKX-NJBDSQKTSA-N ampicillin Chemical compound C1([C@@H](N)C(=O)N[C@H]2[C@H]3SC([C@@H](N3C2=O)C(O)=O)(C)C)=CC=CC=C1 AVKUERGKIZMTKX-NJBDSQKTSA-N 0.000 description 3
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- 102000004190 Enzymes Human genes 0.000 description 2
- 241000237902 Hirudo medicinalis Species 0.000 description 2
- OWYWGLHRNBIFJP-UHFFFAOYSA-N Ipazine Chemical compound CCN(CC)C1=NC(Cl)=NC(NC(C)C)=N1 OWYWGLHRNBIFJP-UHFFFAOYSA-N 0.000 description 2
- 108010054278 Lac Repressors Proteins 0.000 description 2
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- 229960002180 tetracycline Drugs 0.000 description 2
- 229930101283 tetracycline Natural products 0.000 description 2
- 235000019364 tetracycline Nutrition 0.000 description 2
- 150000003522 tetracyclines Chemical class 0.000 description 2
- HMVYERAUBSAVAX-UHFFFAOYSA-N 1-nitro-1-nitrosoguanidine Chemical compound NC(=N)N(N=O)[N+]([O-])=O HMVYERAUBSAVAX-UHFFFAOYSA-N 0.000 description 1
- 101000992180 Acinetobacter baumannii (strain ATCC 19606 / DSM 30007 / JCM 6841 / CCUG 19606 / CIP 70.34 / NBRC 109757 / NCIMB 12457 / NCTC 12156 / 81) Outer membrane protein Omp38 Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- GUBGYTABKSRVRQ-XLOQQCSPSA-N Alpha-Lactose Chemical compound O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@H](O)[C@H](O)[C@H]1O GUBGYTABKSRVRQ-XLOQQCSPSA-N 0.000 description 1
- 229920000945 Amylopectin Polymers 0.000 description 1
- 108091026890 Coding region Proteins 0.000 description 1
- 241001131785 Escherichia coli HB101 Species 0.000 description 1
- 101150102661 HIR1 gene Proteins 0.000 description 1
- 101150040283 HIR2 gene Proteins 0.000 description 1
- 101150019171 HIR3 gene Proteins 0.000 description 1
- 101001086530 Haemophilus influenzae (strain ATCC 51907 / DSM 11121 / KW20 / Rd) Outer membrane protein P5 Proteins 0.000 description 1
- 235000019687 Lamb Nutrition 0.000 description 1
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 1
- 101100506779 Schizosaccharomyces pombe (strain 972 / ATCC 24843) hip1 gene Proteins 0.000 description 1
- 101100017041 Schizosaccharomyces pombe (strain 972 / ATCC 24843) hip3 gene Proteins 0.000 description 1
- 238000012300 Sequence Analysis Methods 0.000 description 1
- 229940122388 Thrombin inhibitor Drugs 0.000 description 1
- 208000007536 Thrombosis Diseases 0.000 description 1
- 108090000631 Trypsin Proteins 0.000 description 1
- 102000004142 Trypsin Human genes 0.000 description 1
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
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- 229960003077 cycloserine Drugs 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
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- 108010087967 type I signal peptidase Proteins 0.000 description 1
- 241001515965 unidentified phage Species 0.000 description 1
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Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/10—Transferases (2.)
- C12N9/1048—Glycosyltransferases (2.4)
- C12N9/1051—Hexosyltransferases (2.4.1)
- C12N9/1074—Cyclomaltodextrin glucanotransferase (2.4.1.19)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P7/00—Drugs for disorders of the blood or the extracellular fluid
- A61P7/02—Antithrombotic agents; Anticoagulants; Platelet aggregation inhibitors
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/81—Protease inhibitors
- C07K14/815—Protease inhibitors from leeches, e.g. hirudin, eglin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
- C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
- C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Medicinal Chemistry (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Gastroenterology & Hepatology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Animal Behavior & Ethology (AREA)
- Hematology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Diabetes (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Materials For Medical Uses (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE4009268A DE4009268A1 (de) | 1990-03-22 | 1990-03-22 | Sekretion von hirudinderivaten |
Publications (3)
Publication Number | Publication Date |
---|---|
NO911137D0 NO911137D0 (no) | 1991-03-21 |
NO911137L NO911137L (no) | 1991-09-23 |
NO308543B1 true NO308543B1 (no) | 2000-09-25 |
Family
ID=6402843
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
NO911137A NO308543B1 (no) | 1990-03-22 | 1991-03-21 | FremgangsmÕte for fremstilling av hirudinderivater, samt rekombinant DNA og rekombinant vektor |
Country Status (23)
Country | Link |
---|---|
US (1) | US5919895A (fi) |
EP (1) | EP0448093B1 (fi) |
JP (1) | JPH07106157B2 (fi) |
KR (1) | KR950007510B1 (fi) |
CN (1) | CN1067104C (fi) |
AT (1) | ATE135042T1 (fi) |
AU (1) | AU640212B2 (fi) |
BR (1) | BR9101104A (fi) |
CA (1) | CA2038888C (fi) |
DE (2) | DE4009268A1 (fi) |
DK (1) | DK0448093T3 (fi) |
ES (1) | ES2084052T3 (fi) |
FI (1) | FI100250B (fi) |
GR (1) | GR3019946T3 (fi) |
HU (1) | HU214368B (fi) |
IE (1) | IE71174B1 (fi) |
IL (1) | IL97323A (fi) |
NO (1) | NO308543B1 (fi) |
NZ (2) | NZ237437A (fi) |
PH (1) | PH30485A (fi) |
PT (1) | PT97093B (fi) |
RU (1) | RU2118365C1 (fi) |
ZA (1) | ZA912013B (fi) |
Families Citing this family (23)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE3835815A1 (de) * | 1988-10-21 | 1990-04-26 | Hoechst Ag | Neue isohirudine |
DE4140381A1 (de) * | 1991-12-07 | 1993-06-09 | Hoechst Ag, 6230 Frankfurt, De | Neue synthetische isohirudine mit verbesserter stabilitaet |
SE9203753D0 (sv) * | 1992-12-11 | 1992-12-11 | Kabi Pharmacia Ab | Expression system for producing apolipoprotein ai-m |
WO1996032409A1 (fr) | 1995-04-12 | 1996-10-17 | Kyowa Hakko Kogyo Co., Ltd. | Facteur de croissance de cellule vegetale |
JP3746847B2 (ja) * | 1996-08-02 | 2006-02-15 | 協和醗酵工業株式会社 | 植物細胞増殖因子 |
DE19915938A1 (de) | 1999-04-09 | 2000-10-19 | Aventis Pharma Gmbh | Herstellung von pankreatischer Procarboxypeptidase B, Isoformen und Muteinen davon und ihre Verwendung |
DE19944870A1 (de) * | 1999-09-18 | 2001-03-29 | Aventis Pharma Gmbh | Signalsequenzen zur Herstellung von Leu-Hirudin über Sekretion durch E. coli in das Kulturmedium |
DE10033195A1 (de) * | 2000-07-07 | 2002-03-21 | Aventis Pharma Gmbh | Bifunktionale Fusionsproteine aus Hirudin und TAP |
US7202059B2 (en) | 2001-02-20 | 2007-04-10 | Sanofi-Aventis Deutschland Gmbh | Fusion proteins capable of being secreted into a fermentation medium |
US7638618B2 (en) | 2001-02-20 | 2009-12-29 | Sanofi-Aventis Deutschland Gmbh | Nucleic acids encoding a hirudin and pro-insulin as superscretable peptides and for parallel improvement of the exported forms of one or more polypeptides of interest |
DE10108211A1 (de) * | 2001-02-20 | 2002-08-22 | Aventis Pharma Gmbh | Verwendung von Fusionsproteinen, deren N-terminaler Anteil aus einem Hirudinderivat besteht, zur Herstellung rekombinanter Proteine über Sekretion durch Hefen |
DE102006004871A1 (de) | 2006-02-02 | 2007-08-09 | Wacker Chemie Ag | Mikroorganismenstamm zur Produktion von rekombinanten Proteinen |
EP1905839B2 (de) * | 2006-09-22 | 2019-07-10 | Wacker Chemie AG | Verfahren zur fermentativen Herstellung von Proteinen |
DK1903105T3 (da) * | 2006-09-22 | 2010-06-28 | Wacker Chemie Ag | Fremgangsmåde til fermentativ fremstilling af proteiner |
DE102006044841A1 (de) | 2006-09-22 | 2008-04-03 | Wacker Chemie Ag | Signalpeptid zur Produktion von rekombinanten Proteinen |
DE502006009060D1 (de) | 2006-09-22 | 2011-04-21 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von Antikörpern |
DE102006050332A1 (de) | 2006-10-25 | 2008-04-30 | Wacker Chemie Ag | DNS-Konstrukt und Verfahren zur fermentativen Herstellung von Fusionsproteinen |
DE102008063900A1 (de) | 2008-12-19 | 2010-06-24 | Wacker Chemie Ag | Verfahren zur fermentativen Herstellung von heterologen Proteinen mittels Escherichia coli |
CN103848914B (zh) * | 2012-11-29 | 2018-08-28 | 河北以岭医药研究院有限公司 | 一种具抗凝活性的菲牛蛭素多肽及其制备方法与用途 |
CN103059130A (zh) * | 2012-12-22 | 2013-04-24 | 浙江工业大学 | 一种水蛭素突变体及重组基因工程菌 |
CN108220369B (zh) * | 2017-12-04 | 2021-03-12 | 广东双骏生物科技有限公司 | 一种生产重组水蛭素的方法 |
WO2020020438A1 (de) | 2018-07-24 | 2020-01-30 | Wacker Chemie Ag | Neue bakterielle lpp-mutante und deren verwendung zur sekretorischen herstellung von rekombinanten proteinen |
CN115572329B (zh) * | 2021-06-21 | 2024-02-06 | 王大勇 | 一组活性增强代谢较慢的菲牛蛭基因重组水蛭素及其制备方法 |
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DE2936543A1 (de) * | 1979-09-10 | 1981-04-09 | Behringwerke Ag, 3550 Marburg | Chromogene verbindungen |
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EP0158564B1 (fr) * | 1984-03-27 | 1992-07-15 | Transgene S.A. | Vecteurs d'expression de l'hirudine, cellules transformées et procédé de préparation de l'hirudine |
DE3438296A1 (de) * | 1984-04-18 | 1985-11-07 | Hoechst Ag, 6230 Frankfurt | Neue polypeptide mit blutgerinnungshemmender wirkung, verfahren zu deren herstellung bzw. gewinnung, deren verwendung und diese enthaltende mittel |
GB8412517D0 (en) * | 1984-05-16 | 1984-06-20 | Nagai K | Recombinant fusion proteins |
ATE64956T1 (de) * | 1984-06-14 | 1991-07-15 | Ciba Geigy Ag | Verfahren zur herstellung von thrombininhibitoren. |
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DE3445517C2 (de) * | 1984-12-13 | 1993-11-18 | Ciba Geigy | Für ein Hirudin-ähnliches Protein codierende DNA-Sequenz und Verfahren zur Herstellung eines Hirudin-ähnlichen Proteins |
US4923808A (en) * | 1985-03-12 | 1990-05-08 | Genentech, Inc. | Method for identifying mutants secreting high levels of heterologous proteins |
DE3900626A1 (de) * | 1985-04-11 | 1989-07-27 | Hoechst Ag | Hirudin-derivat |
FR2593518B1 (fr) * | 1985-05-02 | 1989-09-08 | Transgene Sa | Vecteurs d'expression et de secretion de l'hirudine par les levures transformees |
MY101203A (en) * | 1985-12-12 | 1991-08-17 | Ucp Gen Pharma Ag | Production of thrombin iinhibitors. |
MC1834A1 (fr) * | 1986-07-10 | 1988-06-03 | Transgene Sa | Bloc fonctionnel d'adn et plasmide codant pour l'hirudine,levure transformee,procede de preparation de l'hirudine,hirudine obtenue et son utilisation |
AU604925B2 (en) * | 1988-02-23 | 1991-01-03 | Schering Aktiengesellschaft | A hirudin derivative |
DE3813107A1 (de) * | 1988-04-19 | 1989-11-02 | Consortium Elektrochem Ind | Sekretormutante von escherichia coli |
GB8817160D0 (en) * | 1988-07-19 | 1988-08-24 | Ciba Geigy Ag | Novel proteins |
JPH0648988B2 (ja) * | 1988-07-26 | 1994-06-29 | 工業技術院長 | トロンビン阻害物質の製造法 |
FR2636643B1 (fr) * | 1988-08-24 | 1990-12-28 | Sanofi Sa | Peptide-signal, sequences d'adn codant pour celui-ci, vecteurs d'expression portant l'une de ces sequences et procede de production periplasmique d'un polypeptide |
GB8826428D0 (en) * | 1988-11-11 | 1988-12-14 | Biopharm Ltd | Antithrombin |
US5164304A (en) * | 1989-05-04 | 1992-11-17 | Sri International | Method and vectors for stabilizing hirudin and human laminin b1 expression |
AU648124B2 (en) * | 1990-11-08 | 1994-04-14 | Japan Energy Corporation | Hirudine mutant, production thereof, anticoagulant, secretory vector, microorganism transformed by said vector, and production of product from said microorganism |
-
1990
- 1990-03-22 DE DE4009268A patent/DE4009268A1/de not_active Withdrawn
-
1991
- 1991-02-21 IL IL9732391A patent/IL97323A/xx not_active IP Right Cessation
- 1991-02-22 PH PH42047A patent/PH30485A/en unknown
- 1991-02-28 FI FI911009A patent/FI100250B/fi not_active IP Right Cessation
- 1991-03-05 IE IE73891A patent/IE71174B1/en not_active IP Right Cessation
- 1991-03-14 NZ NZ237437A patent/NZ237437A/xx unknown
- 1991-03-14 NZ NZ245885A patent/NZ245885A/xx unknown
- 1991-03-18 KR KR1019910004246A patent/KR950007510B1/ko not_active IP Right Cessation
- 1991-03-19 ZA ZA912013A patent/ZA912013B/xx unknown
- 1991-03-20 JP JP3056714A patent/JPH07106157B2/ja not_active Expired - Fee Related
- 1991-03-21 CN CN91101687A patent/CN1067104C/zh not_active Expired - Fee Related
- 1991-03-21 AU AU73680/91A patent/AU640212B2/en not_active Ceased
- 1991-03-21 PT PT97093A patent/PT97093B/pt not_active IP Right Cessation
- 1991-03-21 AT AT91104422T patent/ATE135042T1/de not_active IP Right Cessation
- 1991-03-21 RU SU4894968A patent/RU2118365C1/ru not_active IP Right Cessation
- 1991-03-21 NO NO911137A patent/NO308543B1/no unknown
- 1991-03-21 DK DK91104422.0T patent/DK0448093T3/da active
- 1991-03-21 ES ES91104422T patent/ES2084052T3/es not_active Expired - Lifetime
- 1991-03-21 BR BR919101104A patent/BR9101104A/pt not_active Application Discontinuation
- 1991-03-21 HU HU91953A patent/HU214368B/hu not_active IP Right Cessation
- 1991-03-21 EP EP91104422A patent/EP0448093B1/de not_active Expired - Lifetime
- 1991-03-21 DE DE59107495T patent/DE59107495D1/de not_active Expired - Fee Related
- 1991-03-22 CA CA002038888A patent/CA2038888C/en not_active Expired - Fee Related
-
1992
- 1992-11-25 US US07/982,064 patent/US5919895A/en not_active Expired - Fee Related
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1996
- 1996-05-17 GR GR960401313T patent/GR3019946T3/el unknown
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