MX2010010600A - Composiciones y metodos para secuenciacion de acidos nucleicos. - Google Patents

Composiciones y metodos para secuenciacion de acidos nucleicos.

Info

Publication number
MX2010010600A
MX2010010600A MX2010010600A MX2010010600A MX2010010600A MX 2010010600 A MX2010010600 A MX 2010010600A MX 2010010600 A MX2010010600 A MX 2010010600A MX 2010010600 A MX2010010600 A MX 2010010600A MX 2010010600 A MX2010010600 A MX 2010010600A
Authority
MX
Mexico
Prior art keywords
compositions
methods
sequencing
constructs
nucleic acid
Prior art date
Application number
MX2010010600A
Other languages
English (en)
Inventor
Stephen Turner
Kevin Travers
Geoff Otto
Cheryl Heiner
Congcong Ma
Original Assignee
Pacific Biosciences California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41114541&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=MX2010010600(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pacific Biosciences California Inc filed Critical Pacific Biosciences California Inc
Publication of MX2010010600A publication Critical patent/MX2010010600A/es

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Se describen composiciones y métodos para secuenciación de ácido nucleico que incluyen constructos de plantilla que comprenden porciones de doble hebra en constructos parcial o completamente contiguos, para proveer determinación de secuencia redundante por medio de una o ambas de secuenciación de hebras de sentido y anti-sentido y secuenciación iterativamente de todo el constructo múltiples veces. Componentes de secuencia adicionales son también incluidos opcionalmente dentro de tales constructos de plantilla. También se proveen métodos para el uso y preparación de estos constructor, también corno composiciones de secuenciación para su aplicación.
MX2010010600A 2008-03-28 2009-03-27 Composiciones y metodos para secuenciacion de acidos nucleicos. MX2010010600A (es)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7216008P 2008-03-28 2008-03-28
US9969608P 2008-09-24 2008-09-24
PCT/US2009/001926 WO2009120372A2 (en) 2008-03-28 2009-03-27 Compositions and methods for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
MX2010010600A true MX2010010600A (es) 2011-03-30

Family

ID=41114541

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2010010600A MX2010010600A (es) 2008-03-28 2009-03-27 Composiciones y metodos para secuenciacion de acidos nucleicos.

Country Status (9)

Country Link
US (10) US8153375B2 (es)
EP (6) EP4230747A3 (es)
JP (1) JP2011515102A (es)
CN (2) CN104862383B (es)
AU (1) AU2009229157B2 (es)
BR (1) BRPI0909212A2 (es)
CA (2) CA2719747C (es)
MX (1) MX2010010600A (es)
WO (1) WO2009120372A2 (es)

Families Citing this family (309)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
US9121843B2 (en) 2007-05-08 2015-09-01 Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
CA2701794C (en) 2007-10-02 2017-10-31 Theranos, Inc. Modular point-of-care devices and uses thereof
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
AU2009251883B2 (en) * 2008-03-31 2014-09-11 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US9447152B2 (en) * 2008-07-07 2016-09-20 Oxford Nanopore Technologies Limited Base-detecting pore
EP2682460B1 (en) 2008-07-07 2017-04-26 Oxford Nanopore Technologies Limited Enzyme-pore constructs
US20110196142A1 (en) * 2008-09-04 2011-08-11 Biotechnology Research Institute The Chinese Academy of Agricultural Sciences DNA molecule for expressing Hairpin RNA, the constructing method and the use thereof
US8921046B2 (en) * 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
EP2340314B8 (en) 2008-10-22 2015-02-18 Illumina, Inc. Preservation of information related to genomic dna methylation
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
EP2364368B1 (en) 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
US8367813B2 (en) 2008-11-17 2013-02-05 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
WO2010059235A2 (en) * 2008-11-20 2010-05-27 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US9175341B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
US20230148447A9 (en) 2008-12-11 2023-05-11 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
DK2387627T3 (en) 2009-01-15 2016-07-04 Adaptive Biotechnologies Corp Adaptive immunity profiling and methods for producing monoclonal antibodies
AU2010209508C1 (en) 2009-01-30 2017-10-19 Oxford Nanopore Technologies Limited Hybridization linkers
US20120058468A1 (en) 2009-01-30 2012-03-08 Oxford Nanopore Technolgies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
US20100261185A1 (en) 2009-03-27 2010-10-14 Life Technologies Corporation Labeled enzyme compositions, methods and systems
EP2446052B1 (en) 2009-06-25 2018-08-08 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
CA2808576A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
US20110105364A1 (en) * 2009-11-02 2011-05-05 Nugen Technologies, Inc. Compositions and methods for targeted nucleic acid sequence selection and amplification
US9410891B2 (en) 2010-02-19 2016-08-09 Pacific Biosciences Of California, Inc. Optics collection and detection system and method
US20110224106A1 (en) * 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
US9487807B2 (en) 2010-12-27 2016-11-08 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular DNA
CN106290159A (zh) 2011-01-21 2017-01-04 提拉诺斯公司 样品使用最大化的***和方法
EP2670894B1 (en) 2011-02-02 2017-11-29 University Of Washington Through Its Center For Commercialization Massively parallel continguity mapping
CA2826374C (en) 2011-02-11 2024-01-23 Oxford Nanopore Technologies Limited Mutant pores
WO2012121756A1 (en) * 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
WO2012129363A2 (en) 2011-03-24 2012-09-27 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
EP3878975A1 (en) 2011-04-08 2021-09-15 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
US9957560B2 (en) 2011-07-25 2018-05-01 Oxford Nanopore Technologies Ltd. Hairpin loop method for double strand polynucleotide sequencing using transmembrane pores
US10767208B2 (en) 2011-09-06 2020-09-08 Gen-Probe Incorporated Closed nucleic acid structures
EP2753714B1 (en) * 2011-09-06 2017-04-12 Gen-Probe Incorporated Circularized templates for sequencing
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
WO2013049135A1 (en) 2011-09-26 2013-04-04 Gen-Probe Incorporated Algorithms for sequence determinations
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
US9206418B2 (en) 2011-10-19 2015-12-08 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
US9279159B2 (en) 2011-10-21 2016-03-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
CA2858070C (en) 2011-12-09 2018-07-10 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
JP6093498B2 (ja) * 2011-12-13 2017-03-08 株式会社日立ハイテクノロジーズ 核酸増幅方法
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US9404147B2 (en) 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
US10150993B2 (en) 2011-12-22 2018-12-11 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
WO2013102081A2 (en) 2011-12-29 2013-07-04 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013109731A1 (en) * 2012-01-18 2013-07-25 Singular Bio Inc. Methods for mapping bar-coded molecules for structural variation detection and sequencing
CA2861457A1 (en) * 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
DK2823060T3 (en) 2012-03-05 2018-05-28 Adaptive Biotechnologies Corp Determination of associated immune receptor chains from frequency-matched subunits
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
EP2831283A4 (en) 2012-03-30 2015-11-04 Pacific Biosciences California METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
EP2836506B1 (en) 2012-04-10 2017-04-19 Oxford Nanopore Technologies Limited Mutant lysenin pores
EP2836482B1 (en) 2012-04-10 2019-12-25 The Regents of The University of California Compositions and methods for treating cancer
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
ES2582554T3 (es) 2012-05-08 2016-09-13 Adaptive Biotechnologies Corporation Composiciones y método para medir y calibrar el sesgo de la amplificación en reacciones de PCR multiplexadas
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
WO2013185137A1 (en) 2012-06-08 2013-12-12 Pacific Biosciences Of California, Inc. Modified base detection with nanopore sequencing
US9267168B2 (en) 2012-06-12 2016-02-23 Pacific Biosciences Of California, Inc. Methods and compositions for isolating template nucleic acids
SG11201408478QA (en) 2012-06-18 2015-02-27 Nugen Technologies Inc Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
WO2014020137A1 (en) 2012-08-02 2014-02-06 Qiagen Gmbh Recombinase mediated targeted dna enrichment for next generation sequencing
CA2883901C (en) 2012-09-04 2023-04-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20150275267A1 (en) 2012-09-18 2015-10-01 Qiagen Gmbh Method and kit for preparing a target rna depleted sample
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
DK2904111T3 (en) 2012-10-01 2018-03-12 Adaptive Biotechnologies Corp Immune competence evaluation of adaptive immune receptor diversity and clonality characterization
GB201217772D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
US10000802B2 (en) 2012-10-04 2018-06-19 Base4 Innovation Ltd Sequencing method
GB201217770D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
WO2014122288A1 (en) 2013-02-08 2014-08-14 Qiagen Gmbh Method for separating dna by size
CN105209634B (zh) 2013-03-08 2020-05-12 牛津纳米孔技术公司 酶停滞方法
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CN105408908A (zh) * 2013-03-12 2016-03-16 生命科技股份有限公司 用于局部序列比对的方法和***
EP3587592A1 (en) * 2013-03-15 2020-01-01 Theranos IP Company, LLC Nucleic acid amplification
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
EP2971123B1 (en) 2013-03-15 2021-05-05 Labrador Diagnostics LLC Nucleic acid amplification
UY35464A (es) 2013-03-15 2014-10-31 Araxes Pharma Llc Inhibidores covalentes de kras g12c.
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
CN105102627B (zh) * 2013-03-15 2018-10-19 纽约哥伦比亚大学理事会 用于检测样品中多种预定化合物的方法
WO2014145298A1 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nucleic acid amplification
US9822408B2 (en) 2013-03-15 2017-11-21 Nugen Technologies, Inc. Sequential sequencing
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
WO2014182630A1 (en) 2013-05-06 2014-11-13 Pacific Biosciences Of California , Inc. Real-time electronic sequencing
AU2014268322B2 (en) * 2013-05-24 2019-01-24 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed FRET detection
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US9879318B2 (en) 2013-09-06 2018-01-30 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
JO3805B1 (ar) 2013-10-10 2021-01-31 Araxes Pharma Llc مثبطات كراس جي12سي
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
CA2929596C (en) 2013-11-13 2022-07-05 Nugen Technologies, Inc. Compositions and methods for identification of a duplicate sequencing read
JP6673843B2 (ja) 2013-11-17 2020-03-25 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ 分子をプローブし、検出し、分析するための光学システム及びアッセイ・チップ
EP3957750A1 (en) 2013-12-20 2022-02-23 Illumina, Inc. Preserving genomic connectivity information in fragmented genomic dna samples
EP3524694B1 (en) 2013-12-28 2020-07-15 Guardant Health, Inc. Methods and systems for detecting genetic variants
CA2937411C (en) 2014-01-22 2023-09-26 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US9745614B2 (en) 2014-02-28 2017-08-29 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
AU2015227054A1 (en) 2014-03-05 2016-09-22 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
SG11201608486SA (en) * 2014-04-11 2016-11-29 Redvault Biosciences Lp Systems and methods for clonal replication and amplification of nucleic acid molecules for genomic and therapeutic applications
EP3132059B1 (en) 2014-04-17 2020-01-08 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP2940136A1 (en) 2014-04-30 2015-11-04 QIAGEN GmbH Method for isolating poly(A) nucleic acids
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
US10102337B2 (en) 2014-08-06 2018-10-16 Nugen Technologies, Inc. Digital measurements from targeted sequencing
BR112017002489B1 (pt) 2014-08-08 2023-02-14 Quantum-Si Incorporated Instrumento configurado para fazer interface com um chip de teste, aparelho, método de análise de um espécime, método para sequenciar uma molécula-alvo de ácido nucleico e método para sequenciamento de ácido nucleico
CA2957540A1 (en) 2014-08-08 2016-02-11 Quantum-Si Incorporated Integrated device for temporal binning of received photons
CN106796176B (zh) 2014-08-08 2021-02-05 宽腾矽公司 用于对分子进行探测、检测和分析的带外部光源的集成装置
WO2016028887A1 (en) 2014-08-19 2016-02-25 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
CN117164683A (zh) 2014-09-01 2023-12-05 弗拉芒区生物技术研究所 突变csgg孔
ES2726149T3 (es) 2014-09-12 2019-10-02 Mgi Tech Co Ltd Oligonucleótido aislado y su uso en la secuenciación de ácidos nucleicos
CN106715713B (zh) * 2014-09-12 2020-11-03 深圳华大智造科技有限公司 试剂盒及其在核酸测序中的用途
JO3556B1 (ar) 2014-09-18 2020-07-05 Araxes Pharma Llc علاجات مدمجة لمعالجة السرطان
US10011600B2 (en) 2014-09-25 2018-07-03 Araxes Pharma Llc Methods and compositions for inhibition of Ras
AR102094A1 (es) 2014-09-25 2017-02-01 Araxes Pharma Llc Inhibidores de proteínas kras con una mutación g12c
EP3204511B1 (en) 2014-10-07 2021-07-28 Oxford Nanopore Technologies Limited Mutant pores
WO2016057829A1 (en) 2014-10-10 2016-04-14 Quantapore, Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
AU2015335616B2 (en) 2014-10-24 2019-09-12 Quantapore, Inc. Efficient optical analysis of polymers using arrays of nanostructures
ES2784343T3 (es) 2014-10-29 2020-09-24 Adaptive Biotechnologies Corp Detección simultánea altamente multiplexada de ácidos nucleicos que codifican heterodímeros de receptores inmunes adaptativos emparejados de muchas muestras
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
EP3218519B1 (en) * 2014-11-11 2020-12-02 BGI Shenzhen Multi-pass sequencing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
AU2015353581A1 (en) 2014-11-25 2017-06-15 Adaptive Biotechnologies Corporation Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
EP3235010A4 (en) 2014-12-18 2018-08-29 Agilome, Inc. Chemically-sensitive field effect transistor
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
EP3262196B1 (en) 2015-02-24 2020-12-09 Adaptive Biotechnologies Corp. Method for determining hla status using immune repertoire sequencing
DE112016000293T5 (de) * 2015-02-26 2017-09-21 Hitachi High-Technologies Corporation Verfahren zum konstruieren eines nucleinsäure-moleküls
WO2016154345A1 (en) 2015-03-24 2016-09-29 Pacific Biosciences Of California, Inc. Methods and compositions for single molecule composition loading
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
EA201792214A1 (ru) 2015-04-10 2018-01-31 Араксис Фарма Ллк Соединения замещенного хиназолина
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
US10428064B2 (en) 2015-04-15 2019-10-01 Araxes Pharma Llc Fused-tricyclic inhibitors of KRAS and methods of use thereof
US10385387B2 (en) 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
US11983790B2 (en) 2015-05-07 2024-05-14 Pacific Biosciences Of California, Inc. Multiprocessor pipeline architecture
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3304383B1 (en) 2015-05-26 2021-07-07 Pacific Biosciences of California, Inc. De novo diploid genome assembly and haplotype sequence reconstruction
US20180291365A1 (en) * 2015-06-05 2018-10-11 Qiagen Gmbh Method for separating dna by size
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10144724B2 (en) 2015-07-22 2018-12-04 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
US10125391B2 (en) 2015-08-06 2018-11-13 Pacific Biosciences Of California, Inc. Single molecule nanoFET sequencing systems and methods
WO2017058805A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
WO2017058807A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
WO2017058915A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
EP3356353A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
WO2017058768A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
US10882847B2 (en) 2015-09-28 2021-01-05 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356339A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
WO2017070256A2 (en) 2015-10-19 2017-04-27 Araxes Pharma Llc Method for screening inhibitors of ras
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
KR20180081596A (ko) 2015-11-16 2018-07-16 아락세스 파마 엘엘씨 치환된 헤테로사이클릭 그룹을 포함하는 2-치환된 퀴나졸린 화합물 및 이의 사용 방법
US10814299B2 (en) 2015-11-18 2020-10-27 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
ES2911421T3 (es) 2015-12-08 2022-05-19 Twinstrand Biosciences Inc Adaptadores, métodos y composiciones mejorados para secuenciación dúplex
CA3011830A1 (en) 2015-12-08 2017-06-15 Katholieke Universiteit Leuven Ku Leuven Research & Development Modified nanopores, compositions comprising the same, and uses thereof
WO2017100546A1 (en) 2015-12-09 2017-06-15 Araxes Pharma Llc Methods for preparation of quinazoline derivatives
WO2017106768A1 (en) 2015-12-17 2017-06-22 Guardant Health, Inc. Methods to determine tumor gene copy number by analysis of cell-free dna
US10441174B2 (en) 2016-02-17 2019-10-15 Tesseract Health, Inc. Sensor and device for lifetime imaging and detection applications
EP4015531A3 (en) 2016-03-02 2022-09-28 Oxford Nanopore Technologies PLC Mutant pore
US11384380B2 (en) 2016-03-16 2022-07-12 Yale University Compositions and methods for detecting N6-methyladenine in the mammalian genome
ES2914396T3 (es) * 2016-03-22 2022-06-10 Vib Vzw Medios y métodos de amplificación de secuencias de nucleótidos
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
EP4282974A3 (en) 2016-03-28 2024-03-13 Ncan Genomics, Inc. Linked duplex target capture
US10822312B2 (en) 2016-03-30 2020-11-03 Araxes Pharma Llc Substituted quinazoline compounds and methods of use
EP3440222A1 (en) 2016-04-04 2019-02-13 H. Hoffnabb-La Roche Ag Nucleic acid sample preparation methods
AU2017246690B2 (en) 2016-04-06 2022-03-24 Oxford Nanopore Technologies Plc Mutant pore
EP3443066A4 (en) 2016-04-14 2019-12-11 Guardant Health, Inc. EARLY DETECTION METHODS FOR CANCER
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
EP3459115A4 (en) 2016-05-16 2020-04-08 Agilome, Inc. GRAPHEN-FET DEVICES, SYSTEMS AND METHODS FOR USE THEREOF FOR SEQUENCING NUCLEIC ACIDS
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017209891A1 (en) * 2016-05-31 2017-12-07 Quantapore, Inc. Two-color nanopore sequencing
CN107488656B (zh) * 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
CN107557355A (zh) 2016-07-01 2018-01-09 Pgi股份有限公司 建构环状模板和检测dna分子的方法
WO2018009346A1 (en) 2016-07-05 2018-01-11 Quantapore, Inc. Optically based nanopore sequencing
US10646488B2 (en) 2016-07-13 2020-05-12 Araxes Pharma Llc Conjugates of cereblon binding compounds and G12C mutant KRAS, HRAS or NRAS protein modulating compounds and methods of use thereof
JP6889769B2 (ja) * 2016-07-18 2021-06-18 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 核酸配列決定の非対称な鋳型および非対称な方法
CN109312391B (zh) * 2016-07-18 2022-06-03 豪夫迈·罗氏有限公司 生成用于单分子测序的单链环状dna文库的方法
US10711300B2 (en) 2016-07-22 2020-07-14 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
WO2018033730A1 (en) * 2016-08-16 2018-02-22 Touchlight IP Limited Closed linear dna production
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
US10280172B2 (en) 2016-09-29 2019-05-07 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
CA3126055A1 (en) 2016-09-30 2018-04-05 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US10377743B2 (en) 2016-10-07 2019-08-13 Araxes Pharma Llc Inhibitors of RAS and methods of use thereof
JP6941680B2 (ja) 2016-10-24 2021-09-29 ジェネインフォセック、インコーポレイテッド 核酸内に存在する情報の秘匿化
WO2018104908A2 (en) 2016-12-09 2018-06-14 Boreal Genomics, Inc. Linked ligation
CA3047125A1 (en) 2016-12-15 2018-06-21 The Regents Of The University Of California Compositions and methods for treating cancer
JP2020501573A (ja) 2016-12-19 2020-01-23 クアンタム−エスアイ インコーポレイテッドＱｕａｎｔｕｍ−Ｓｉ Ｉｎｃｏｒｐｏｒａｔｅｄ シークエンシング反応用の重合酵素
EP3559269B1 (en) 2016-12-20 2020-09-16 F. Hoffmann-La Roche AG Single stranded circular dna libraries for circular consensus sequencing
BR112019012540A2 (pt) 2016-12-22 2019-11-12 Quantum-Si Incorporated fotodetector integrado com pixel de acondicionamento direto
WO2018140512A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc Fused bicyclic benzoheteroaromatic compounds and methods of use thereof
CN110382482A (zh) 2017-01-26 2019-10-25 亚瑞克西斯制药公司 稠合的杂-杂二环化合物及其使用方法
WO2018140599A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc Benzothiophene and benzothiazole compounds and methods of use thereof
US11279689B2 (en) 2017-01-26 2022-03-22 Araxes Pharma Llc 1-(3-(6-(3-hydroxynaphthalen-1-yl)benzofuran-2-yl)azetidin-1 yl)prop-2-en-1-one derivatives and similar compounds as KRAS G12C modulators for treating cancer
WO2018140514A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc 1-(6-(3-hydroxynaphthalen-1-yl)quinazolin-2-yl)azetidin-1-yl)prop-2-en-1-one derivatives and similar compounds as kras g12c inhibitors for the treatment of cancer
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
AU2018212756B2 (en) * 2017-01-27 2021-02-25 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
CN106906211B (zh) * 2017-04-13 2020-11-20 苏州普瑞迈德医学检验所有限公司 一种分子接头及其应用
MX2019013111A (es) 2017-05-05 2019-12-16 Quantum Si Inc Sustratos que tienen reactividad de superficie modificada y propiedades antiincrustantes en reacciones biologicas.
TW201906832A (zh) 2017-05-25 2019-02-16 美商亞瑞克西斯製藥公司 用於癌症治療之化合物及其使用方法
WO2018218069A1 (en) 2017-05-25 2018-11-29 Araxes Pharma Llc Quinazoline derivatives as modulators of mutant kras, hras or nras
MX2019013954A (es) 2017-05-25 2020-08-31 Araxes Pharma Llc Inhibidores covalentes de kras.
CN107423554B (zh) * 2017-06-09 2020-02-18 东南大学 一种用可逆单分子反应实现马尔科夫链的设计方法
EP4273156A3 (en) 2017-06-30 2024-01-17 Vib Vzw Novel protein pores
EP3655547A4 (en) 2017-07-18 2021-03-24 Pacific Biosciences Of California, Inc. METHODS AND COMPOSITIONS FOR ISOLATING ASYMMETRICAL NUCLEIC ACID COMPLEXES
CN111148850A (zh) 2017-07-24 2020-05-12 宽腾矽公司 高强度经标记的反应物组合物及用于测序的方法
JP2020532976A (ja) 2017-09-14 2020-11-19 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ 環状一本鎖ｄｎａライブラリーを生成するための新規な方法
WO2019053215A1 (en) 2017-09-15 2019-03-21 F. Hoffmann-La Roche Ag HYBRIDIZATION-EXTENSION-LIGATURE STRATEGY TO GENERATE CIRCULAR SINGLE-STRAND DNA BANKS
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
JP7083393B2 (ja) 2017-10-06 2022-06-10 エフ．ホフマン－ラ ロシュ アーゲー 単一分子配列決定の試料調製のための環化方法
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
WO2019086531A1 (en) 2017-11-03 2019-05-09 F. Hoffmann-La Roche Ag Linear consensus sequencing
SG11202003885UA (en) 2017-11-08 2020-05-28 Twinstrand Biosciences Inc Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
EP3724353A1 (en) * 2017-12-11 2020-10-21 UMC Utrecht Holding B.V. Methods for preparing nucleic acid molecules for sequencing
EP3729090A4 (en) 2017-12-22 2021-09-22 Pacific Biosciences Of California, Inc. MODIFIED BIOTIN-BINDING PROTEINS FOR IMMOBILIZATION
WO2019149958A1 (en) 2018-02-05 2019-08-08 F. Hoffmann-La Roche Ag Generation of single-stranded circular dna templates for single molecule
CN108359723B (zh) * 2018-02-23 2021-06-08 奥明（杭州）基因科技有限公司 一种降低深度测序错误的方法
CN111868257A (zh) 2018-03-02 2020-10-30 豪夫迈·罗氏有限公司 用于单分子测序的双链dna模板的生成
CN112041462B (zh) 2018-03-23 2024-06-07 德克萨斯州立大学董事会 具有极低的错误水平的dsDNA有效测序
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
US20210189460A1 (en) * 2018-04-25 2021-06-24 Qiagen Sciences Llc Sequential paired-end sequencing
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
WO2019226689A1 (en) 2018-05-22 2019-11-28 Axbio Inc. Methods, systems, and compositions for nucleic acid sequencing
US20210381041A1 (en) 2018-05-28 2021-12-09 Roche Sequencing Solutions, Inc. Enzymatic Enrichment of DNA-Pore-Polymerase Complexes
EP3811610A1 (en) 2018-06-22 2021-04-28 Quantum-Si Incorporated Integrated photodetector with charge storage bin of varied detection time
EP3814531A4 (en) 2018-06-29 2022-04-06 Pacific Biosciences Of California, Inc. METHODS AND COMPOSITIONS FOR DELIVERING MOLECULES AND COMPLEXES TO REACTION SITES
US20210375391A1 (en) 2018-06-29 2021-12-02 Roche Sequencing Solutions, Inc Detection of microsatellite instability
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
US20210317517A1 (en) 2018-08-28 2021-10-14 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
WO2020102766A2 (en) * 2018-11-15 2020-05-22 Element Biosciences, Inc. Methods for generating circular nucleic acid molecules
CA3117889A1 (en) 2018-11-15 2020-05-22 Quantum-Si Incorporated Methods and compositions for protein sequencing
KR20210098491A (ko) 2018-11-30 2021-08-10 진인포섹, 아이엔씨. 무작위 올리고뉴클레오티드를 생성하고 그 서열을 결정하는 방법
EP3674702A1 (en) 2018-12-27 2020-07-01 Imec VZW Method for sequencing a polynucleotide using a biofet
US11473136B2 (en) 2019-01-03 2022-10-18 Ncan Genomics, Inc. Linked target capture
US20220098642A1 (en) * 2019-01-04 2022-03-31 William Marsh Rice University Quantitative amplicon sequencing for multiplexed copy number variation detection and allele ratio quantitation
US11613772B2 (en) 2019-01-23 2023-03-28 Quantum-Si Incorporated High intensity labeled reactant compositions and methods for sequencing
WO2020157684A1 (en) * 2019-01-29 2020-08-06 Mgi Tech Co., Ltd. High coverage stlfr
EP3918089A1 (en) 2019-01-31 2021-12-08 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
CN114364829A (zh) * 2019-06-26 2022-04-15 北京贝瑞和康生物技术有限公司 一种构建PacBio测序文库的方法
EP3976771A1 (en) 2019-06-28 2022-04-06 Quantum-si Incorporated Polymerizing enzymes for sequencing reactions
US20220340954A1 (en) 2019-06-28 2022-10-27 Qiagen Gmbh Method for separating nucleic acid molecules by size
WO2021032660A1 (en) 2019-08-19 2021-02-25 F. Hoffmann-La Roche Ag Single tube preparation of dna and rna for sequencing
EP4031679A1 (en) 2019-09-20 2022-07-27 F. Hoffmann-La Roche AG Immune repertoire profiling by primer extension target enrichment
EP4028570A1 (en) 2019-10-11 2022-07-20 Quantum-Si Incorporated Surface modification in the vapor phase
CA3159566A1 (en) 2019-10-29 2021-05-06 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
US11358981B2 (en) 2020-01-21 2022-06-14 Quantum-Si Incorporated Compounds and methods for selective c-terminal labeling
EP4114966A1 (en) 2020-03-03 2023-01-11 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
JP7490071B2 (ja) 2020-03-11 2024-05-24 エフ． ホフマン－ラ ロシュ アーゲー シーケンシングのための新規核酸鋳型構造
WO2022029449A1 (en) 2020-08-07 2022-02-10 Oxford Nanopore Technologies Limited Methods of identifying nucleic acid barcodes
US20220177950A1 (en) 2020-12-03 2022-06-09 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CN112680320A (zh) * 2021-01-21 2021-04-20 季华实验室 零模波导孔结构及其制备方法、以及其使用方法
JP2024506899A (ja) 2021-02-09 2024-02-15 エフ． ホフマン－ラ ロシュ アーゲー 核酸中のメチル化の塩基レベル検出のための方法
EP4301839A1 (en) 2021-03-03 2024-01-10 F. Hoffmann-La Roche AG Devices and methods for electrophoretic extraction of nucleic acids from biological samples
WO2022248237A1 (en) 2021-05-24 2022-12-01 F. Hoffmann-La Roche Ag Enhancer oligonucleotides for nucleic acid hybridization
WO2023283347A1 (en) * 2021-07-08 2023-01-12 Pacific Biosciences Of California, Inc. Paired-end sequencing methods and compositions
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
WO2023242075A1 (en) 2022-06-14 2023-12-21 F. Hoffmann-La Roche Ag Detection of epigenetic cytosine modification
WO2023245203A1 (en) * 2022-06-18 2023-12-21 Reticula, Inc. Methods for single cell sequencing and error rate reduction
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors

Family Cites Families (253)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2433109A (en) 1937-03-16 1947-12-23 Fouron Pierre Chill mould for casting cylinder heads of air-cooled engines
US3873209A (en) 1973-12-10 1975-03-25 Bell Telephone Labor Inc Measurement of thin films by optical waveguiding technique
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5270201A (en) 1988-03-24 1993-12-14 The General Hospital Corporation Artificial chromosome vector
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5622868A (en) 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
EP0450060A1 (en) 1989-10-26 1991-10-09 Sri International Dna sequencing
IL97222A (en) 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and Responder for Determining Special Changes to Nucleotide
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
DE59109246D1 (de) 1990-05-03 2003-04-03 Hoffmann La Roche Mikrooptischer Sensor
DE69132843T2 (de) 1990-12-06 2002-09-12 Affymetrix Inc N D Ges D Staat Identifizierung von Nukleinsäuren in Proben
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5162654A (en) * 1991-02-01 1992-11-10 Wisconsin Alumni Research Foundation Detection apparatus for electrophoretic gels
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
FR2675803B1 (fr) 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
US5365455A (en) * 1991-09-20 1994-11-15 Vanderbilt University Method and apparatus for automatic nucleic acid sequence determination
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5243618A (en) 1991-11-22 1993-09-07 Hughes Aircraft Company Cavity resonator incorporating waveguide filter
WO1993017127A1 (en) 1992-02-20 1993-09-02 The State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University Boomerand dna amplification
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US5350686A (en) 1992-04-16 1994-09-27 Pioneer Hi-Bred International, Inc. Microwave acceleration of enzyme-catalyzed modification of macromolecules
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
US5547835A (en) 1993-01-07 1996-08-20 Sequenom, Inc. DNA sequencing by mass spectrometry
US5465151A (en) 1993-01-21 1995-11-07 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Sensors employing interference of electromagnetic waves passing through waveguides having functionalized surfaces
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5677196A (en) 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
WO1995006138A1 (en) 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5470710A (en) 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5629468A (en) * 1994-05-26 1997-05-13 Universtiy Of Tennessee Research Corporation Root stimulating bacteria
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
FR2729392B1 (fr) * 1995-01-16 1997-03-28 Pasteur Institut Oligonucleotides pour la detection de salmonella
US5601982A (en) 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US6103465A (en) * 1995-02-14 2000-08-15 The Perkin-Elmer Corporation Methods and reagents for typing HLA class I genes
AU5171696A (en) 1995-02-27 1996-09-18 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5674683A (en) 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5961923A (en) 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5677769A (en) 1995-05-30 1997-10-14 Imra America Optical sensor utilizing rare-earth-doped integrated-optic lasers
EP0745686A1 (en) 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
WO1997004131A1 (en) 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US5661028A (en) 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
DK0862656T3 (da) * 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6611634B2 (en) 1996-03-19 2003-08-26 University Of Utah Research Foundation Lens and associatable flow cell
JP2000512744A (ja) 1996-05-16 2000-09-26 アフィメトリックス，インコーポレイテッド 標識材料を検出するシステムおよび方法
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
CN1235673A (zh) 1996-09-30 1999-11-17 阿温提斯研究技术两合公司 探测溶解或分散在水中的化学物质的光传感器
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6136543A (en) 1997-01-31 2000-10-24 Hitachi, Ltd. Method for determining nucleic acids base sequence and apparatus therefor
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
ATE273381T1 (de) 1997-02-12 2004-08-15 Eugene Y Chan Verfahren zur analyse von polymeren
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
EP1498494A3 (en) 1997-04-01 2007-06-20 Solexa Ltd. Method of nucleic acid sequencing
US5948616A (en) * 1997-05-14 1999-09-07 Musc Foundation For Research Development Methods and compositions of correlating tissue kallikrein gene promoter polymorphisms with essential hypertension
CA2294962C (en) 1997-07-28 2012-09-18 Medical Biosystems Ltd. Nucleic acid sequence analysis
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
CA2305449A1 (en) 1997-10-10 1999-04-22 President & Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP3862845B2 (ja) 1998-02-05 2006-12-27 セイコーインスツル株式会社 近接場用光プローブ
US6049641A (en) 1998-02-24 2000-04-11 Gemfire Corporation Connection system for optical redundancy
WO1999053102A1 (en) 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
GB9810350D0 (en) 1998-05-14 1998-07-15 Ciba Geigy Ag Organic compounds
US6326725B1 (en) 1998-05-26 2001-12-04 Micron Technology, Inc. Focusing electrode for field emission displays and method
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
AU4355899A (en) 1998-06-26 2000-01-17 Visible Genetics Inc. Method for sequencing nucleic acids with reduced errors
GB9815702D0 (en) 1998-07-21 1998-09-16 Cambridge Imaging Ltd Improved imaging system for fluorescence assays
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
JP2002521064A (ja) 1998-07-30 2002-07-16 ソレックサ リミテッド アレイ生体分子およびシークエンシングにおけるその使用
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
WO2000009757A1 (en) 1998-08-13 2000-02-24 U.S. Genomics, Inc. Optically characterizing polymers
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6280939B1 (en) 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
US6203985B1 (en) 1998-09-08 2001-03-20 Motorola, Inc. Bio-molecule analyzer with photosensitive material and fabrication
US6287824B1 (en) 1998-09-15 2001-09-11 Yale University Molecular cloning using rolling circle amplification
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
DE19844931C1 (de) * 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
US6232075B1 (en) 1998-12-14 2001-05-15 Li-Cor, Inc. Heterogeneous assay for pyrophosphate detection
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9828785D0 (en) 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
US6635470B1 (en) 1999-01-08 2003-10-21 Applera Corporation Fiber array and methods for using and making same
EP1149175A2 (en) 1999-01-11 2001-10-31 President And Fellows Of Harvard College Isothermal amplification of dna
EP1157144A4 (en) 1999-01-13 2010-04-28 Cornell Res Foundation Inc MANUFACTURE OF MONOLITHIC FLUID STRUCTURES
US6613513B1 (en) 1999-02-23 2003-09-02 Caliper Technologies Corp. Sequencing by incorporation
ES2310514T3 (es) 1999-03-10 2009-01-16 Asm Scientific, Inc. Un metodo para la secuenciacion directa de acido nucleico.
US6515751B1 (en) 1999-03-11 2003-02-04 Cornell Research Foundation Inc. Mechanically resonant nanostructures
AU3567900A (en) 1999-03-30 2000-10-16 Solexa Ltd. Polynucleotide sequencing
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6056661A (en) 1999-06-14 2000-05-02 General Motors Corporation Multi-range transmission with input split planetary gear set and continuously variable transmission unit
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
IL148088A0 (en) 1999-08-13 2002-09-12 Us Genomics Inc Methods and apparatuses for stretching polymers
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
EP1218543A2 (en) 1999-09-29 2002-07-03 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
AU2573801A (en) 1999-11-02 2001-05-14 University Of Hawaii Method for fabricating arrays of micro-needles
WO2001032930A1 (en) 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6324327B1 (en) 1999-11-23 2001-11-27 Corning Incorporated Low dispersion slope negative dispersion optical fiber
CN1336960A (zh) * 1999-11-26 2002-02-20 株式会社百尼尔 采用多种dna聚合酶的dna测序方法和所用的试剂盒
JP2003532380A (ja) 1999-12-02 2003-11-05 モレキュラー ステージング，インコーポレイテッド 線状自己アニーリングセグメントからの一本鎖環状ｄｎａの産出
ATE317024T1 (de) 1999-12-29 2006-02-15 Keygene Nv Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden
GB0000896D0 (en) 2000-01-14 2000-03-08 Univ Glasgow Improved analytical chip
US6510263B1 (en) 2000-01-27 2003-01-21 Unaxis Balzers Aktiengesellschaft Waveguide plate and process for its production and microtitre plate
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
AU2001241474A1 (en) * 2000-02-11 2001-08-20 The Texas A And M University System Biosensor compositions and methods of use
DE10013254A1 (de) 2000-03-17 2001-10-04 Friz Biochem Gmbh Vorrichtung und Verfahren zum Nachweis organischer Moleküle in einer Probensubstanz
US20030213771A1 (en) 2000-03-31 2003-11-20 Kenichirou Ohshita Surface treatment method for magnesium alloys and magnesium alloy members thus treated
EP1280928B1 (en) * 2000-05-10 2016-11-30 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Resistance to acetohydroxyacid synthase-inhibiting herbicides
US6291187B1 (en) 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
KR100867760B1 (ko) 2000-05-15 2008-11-10 소니 가부시끼 가이샤 재생장치, 재생방법 및 기록매체
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US20030027308A1 (en) 2000-05-30 2003-02-06 Sugen Incorporated Novel human protein phosphatases identified from genomic sequencing
AU2001274068A1 (en) 2000-06-02 2001-12-11 Zeptosens Ag Kit and method for determining a plurality of analytes
WO2001094609A1 (en) 2000-06-07 2001-12-13 Li-Cor, Inc. Charge-switch nucleotides
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
EP2100971A3 (en) * 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
WO2002029106A2 (en) 2000-10-03 2002-04-11 California Institute Of Technology Microfluidic devices and methods of use
US20020180570A1 (en) 2000-10-26 2002-12-05 The Trustees Of Princeton University Method and apparatus for dielectric spectroscopy or biological solustions
TWI316964B (es) 2000-10-30 2009-11-11 Takara Bio Inc
US20040157306A1 (en) 2000-11-13 2004-08-12 Sugen Incorporated Mammalian protein phosphatases
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
GB2390155B (en) 2001-01-03 2004-04-21 Packard Instrument Co Inc Luminescence imager
US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
DE10102578C2 (de) 2001-01-20 2003-01-09 Univ Braunschweig Tech Resonanter Mikrowellensensor
GB2389904A (en) 2001-01-30 2003-12-24 Solexa Ltd Arrayed polynucleotides and their use in genome analysis
JP2002237200A (ja) 2001-02-13 2002-08-23 Mitsubishi Electric Corp 半導体装置およびその検査方法
WO2002072892A1 (en) 2001-03-12 2002-09-19 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences by asynchronous base extension
US20030143556A1 (en) 2001-04-03 2003-07-31 Gary Blackburn Nucleic acid reactions using labels with different redox potentials
EP1419264A2 (en) 2001-04-06 2004-05-19 Incyte Genomics, Inc. Kinases and phosphatases
AU2002258997A1 (en) 2001-04-24 2002-11-05 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymerases
CA2344599C (en) 2001-05-07 2011-07-12 Bioneer Corporation Selective polymerase chain reaction of dna of which base sequence is completely unknown
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
WO2002094846A2 (en) 2001-05-22 2002-11-28 Parallel Synthesis Technologies, Inc. Method for in situ, on-chip chemical synthesis
US20040203097A1 (en) 2001-05-24 2004-10-14 Henry Yue Kinases and phosphatases
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US20020187508A1 (en) 2001-06-08 2002-12-12 Wong Gordon G. Methods and products for analyzing nucleic acids using nick translation
CA2454570C (en) 2001-07-25 2016-12-20 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
EP1421213B1 (en) 2001-08-29 2010-02-17 Amersham Biosciences Corp. Labeled nucleoside polyphosphates
US6852492B2 (en) 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
AU2002343792A1 (en) * 2001-11-28 2003-06-10 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
US6713672B1 (en) 2001-12-07 2004-03-30 Laird Technologies, Inc. Compliant shaped EMI shield
JP2003185569A (ja) 2001-12-14 2003-07-03 Mitsubishi Chemicals Corp 表面プラズモン共鳴を利用した試料の分析装置及び表面プラズモン共鳴分析用センサチップ
US20030123827A1 (en) 2001-12-28 2003-07-03 Xtalight, Inc. Systems and methods of manufacturing integrated photonic circuit devices
JP3897703B2 (ja) 2002-01-11 2007-03-28 キヤノン株式会社 センサ装置およびそれを用いた検査方法
US6618537B2 (en) 2002-01-14 2003-09-09 Applied Wdm, Inc. Optical waveguide structures and methods of fabrication
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
CA2443023A1 (en) * 2002-02-01 2003-08-07 King Pharmaceuticals Research And Development, Inc. 8-heteroaryl xanthine adenosine a2b receptor antagonists
DE10217568A1 (de) 2002-04-19 2003-11-13 Infineon Technologies Ag Wellenleiter in porösen Substraten
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
US20050019784A1 (en) 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
JP2003344433A (ja) 2002-05-22 2003-12-03 Okutekku:Kk マイクロアレイ、マイクロアレイシステム及び被検物質の測定方法
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
JP4480715B2 (ja) * 2003-01-29 2010-06-16 ４５４ コーポレーション 二重末端シーケンシング
WO2004071155A2 (en) 2003-02-05 2004-08-26 Amersham Biosciences Corp Solid phase sequencing
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US20060147935A1 (en) 2003-02-12 2006-07-06 Sten Linnarsson Methods and means for nucleic acid sequencing
WO2004113957A2 (en) 2003-06-16 2004-12-29 The Regents Of The University Of California Apparatus for optical measurements on low-index non-solid materials based on arrow waveguides
US20050069938A1 (en) * 2003-09-26 2005-03-31 Youxiang Wang Amplification of polynucleotides by rolling circle amplification
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
WO2005049848A2 (en) 2003-11-12 2005-06-02 Geneohm Sciences, Inc. Nucleic acid hybridization methods
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
JP2005218385A (ja) 2004-02-06 2005-08-18 Dnaform:Kk １本鎖ｄｎａを調製するための方法
US7981604B2 (en) 2004-02-19 2011-07-19 California Institute Of Technology Methods and kits for analyzing polynucleotide sequences
US20100216151A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
US7238485B2 (en) * 2004-03-23 2007-07-03 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060024711A1 (en) * 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7256886B2 (en) 2004-07-22 2007-08-14 University Of Maryland At Baltimore County Surface enhanced Raman spectroscopic nano-imaging probe and uses therefor
CN101914620B (zh) 2004-09-17 2014-02-12 加利福尼亚太平洋生命科学公司 核酸测序的方法
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7424371B2 (en) 2004-12-21 2008-09-09 Helicos Biosciences Corporation Nucleic acid analysis
US7700287B2 (en) 2005-01-28 2010-04-20 Life Technologies Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template
JP4415266B2 (ja) 2005-02-01 2010-02-17 住友電装株式会社 シールドコネクタ
JP2006333739A (ja) 2005-05-31 2006-12-14 Hitachi High-Technologies Corp 単分子計測による核酸分析方法
US7601499B2 (en) 2005-06-06 2009-10-13 454 Life Sciences Corporation Paired end sequencing
WO2007003017A1 (en) 2005-07-06 2007-01-11 Biochip Innovations Pty Ltd A method and kit for analyzing a target nucleic acid sequence
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
WO2007014397A2 (en) 2005-07-28 2007-02-01 Helicos Biosciences Corporation Consecutive base single molecule sequencing
US7405381B2 (en) 2005-08-23 2008-07-29 Cem, Corporation Real-time imaging and spectroscopy during microwave assisted chemistry
US7666593B2 (en) * 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
JP2009511862A (ja) 2005-09-30 2009-03-19 パシフィック バイオサイエンシーズ オブ カリフォルニア， インコーポレイテッド 反応性表面、基質および同表面、基質の製造および使用方法
US20070087358A1 (en) * 2005-10-19 2007-04-19 Melanie Ehrlich Methods for diagnosing cancer based on DNA methylation status in NBL2
EP1969153A2 (en) 2005-11-28 2008-09-17 Pacific Biosciences of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
EP1960550B1 (en) 2005-12-12 2010-09-15 The Government of the United States of America as represented by The Secretary of the Department of Health and Human Services Probe for nucleic acid sequencing and methods of use
US20090305248A1 (en) 2005-12-15 2009-12-10 Lander Eric G Methods for increasing accuracy of nucleic acid sequencing
US20100260465A1 (en) 2005-12-22 2010-10-14 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
ATE518010T1 (de) 2005-12-22 2011-08-15 Pacific Biosciences California Aktive oberflächengekoppelte polymerasen
EP3056575B1 (en) 2005-12-22 2017-10-11 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
US7368265B2 (en) 2006-01-23 2008-05-06 Compass Genetics, Llc Selective genome amplification
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
US7608034B2 (en) * 2006-02-24 2009-10-27 Powis Parker Inc. Stack conditioning apparatus and method for use in bookbinding
EP1994180A4 (en) 2006-02-24 2009-11-25 Callida Genomics Inc GENOME SEQUENCING ON DNA ARRAYS WITH HIGH THROUGHPUT
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US20090075252A1 (en) 2006-04-14 2009-03-19 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7282337B1 (en) 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7897747B2 (en) 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
EP2029780A4 (en) 2006-06-16 2010-03-31 Pacific Biosciences California CONTROLLED INITIATION OF A PRIMARY EXTENSION
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080108804A1 (en) * 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
US20090111706A1 (en) 2006-11-09 2009-04-30 Complete Genomics, Inc. Selection of dna adaptor orientation by amplification
AU2008261935B2 (en) 2007-06-06 2013-05-02 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US7901889B2 (en) 2007-07-26 2011-03-08 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
WO2009089384A1 (en) 2008-01-09 2009-07-16 Life Technologies Method of making a paired tag library for nucleic acid sequencing
US7767400B2 (en) 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis
MX2010010600A (es) 2008-03-28 2011-03-30 Pacific Biosciences California Inc Composiciones y metodos para secuenciacion de acidos nucleicos.
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
WO2009132315A1 (en) 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
WO2010036287A1 (en) 2008-09-24 2010-04-01 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US20120058468A1 (en) 2009-01-30 2012-03-08 Oxford Nanopore Technolgies Limited Adaptors for nucleic acid constructs in transmembrane sequencing
US9969608B2 (en) 2016-01-05 2018-05-15 DunAn Sensing, LLC Methods for mounting a MEMS sensor for in-stream measurements

Also Published As

Publication number Publication date
WO2009120372A3 (en) 2010-01-07
US20160321397A1 (en) 2016-11-03
US9582640B2 (en) 2017-02-28
CN102084001A (zh) 2011-06-01
US20160376647A1 (en) 2016-12-29
US8455193B2 (en) 2013-06-04
EP2274449B1 (en) 2015-12-23
US9600626B2 (en) 2017-03-21
EP3170904A1 (en) 2017-05-24
US11705217B2 (en) 2023-07-18
US9404146B2 (en) 2016-08-02
WO2009120372A2 (en) 2009-10-01
EP4230747A2 (en) 2023-08-23
JP2011515102A (ja) 2011-05-19
EP2274449A2 (en) 2011-01-19
US8309330B2 (en) 2012-11-13
US20180211003A1 (en) 2018-07-26
US20160237485A1 (en) 2016-08-18
EP3269824A1 (en) 2018-01-17
US20130309678A1 (en) 2013-11-21
CA2991818A1 (en) 2009-10-01
CN102084001B (zh) 2015-03-18
EP3170904B1 (en) 2017-08-16
US20110281768A1 (en) 2011-11-17
US20120289408A1 (en) 2012-11-15
US8153375B2 (en) 2012-04-10
BRPI0909212A2 (pt) 2015-08-18
US20090298075A1 (en) 2009-12-03
AU2009229157B2 (en) 2015-01-29
EP3425060A1 (en) 2019-01-09
US9542527B2 (en) 2017-01-10
CA2719747A1 (en) 2009-10-01
CA2991818C (en) 2022-10-11
US20150159196A1 (en) 2015-06-11
EP4230747A3 (en) 2023-11-15
EP3045542A1 (en) 2016-07-20
US20160348167A1 (en) 2016-12-01
US9910956B2 (en) 2018-03-06
CN104862383A (zh) 2015-08-26
CA2719747C (en) 2018-02-20
EP3425060B1 (en) 2021-10-27
CN104862383B (zh) 2019-05-28
AU2009229157A1 (en) 2009-10-01
EP2274449A4 (en) 2012-04-18
EP3045542B1 (en) 2016-11-16

Similar Documents

Publication Publication Date Title
MX2010010600A (es) Composiciones y metodos para secuenciacion de acidos nucleicos.
AU2017248555B2 (en) Closed nucleic acid structures
WO2012009644A3 (en) Methods to identify synthetic and natural rna elements that enhance protein translation
EP1941050A4 (en) METHODS AND COMPOSITIONS FOR PRODUCING SINGLE-SEQUENCE DNA PROBES, DNA-PROBE LABELING AND USE THEREOF
WO2011026128A3 (en) Flowcells and methods of filling and using same
WO2012138487A3 (en) Oligonucleotide modulation of splicing
BRPI0807132A2 (pt) enzimas para o tratamento de lignocelulósicos, ácidos nucléicos que codificam as mesmas, e metodos e uso das mesmas
WO2009111643A3 (en) Microrna markers for recurrence of colorectal cancer
WO2011084803A3 (en) Coloring agents and methods of use thereof
TN2010000213A1 (en) Wise binding antibodies and epitopes
UA104459C2 (uk) Антитіла проти fgfr3 і способи їх застосування
HK1190306A1 (zh) 含有心源性因子的組合物和相關方法、細胞，以及所述組合物、方法和細胞的應用
BR112013007229A2 (pt) ''composição,mistura e processo''.
WO2009149956A3 (en) Fusion protein and use thereof
BRPI1011423A2 (pt) composição injetável, kit e método cosmético.
MX2013009175A (es) Composiciones que comprenden acido peroxi alfa-cetocarboxilico y metodos para producirlas y usarlas.
WO2009002440A3 (en) Compositions comprising human egfr-sirna and methods of use
WO2009108217A3 (en) Compositions comprising k-ras sirna and methods of use
WO2009037473A3 (en) Nucleobase characterisation
GB2489187B (en) Compositions, methods and related uses for cleaving modified DNA
WO2009150255A3 (en) Markers for predicting response and survival in anti-egfr treated patients
WO2011160052A3 (en) Methods and compositions for sequence specific rna endonucleases
ITTO20091050A1 (it) Sostanze coloranti e loro utilizzazione in composizioni, in particolare in composizioni cosmetiche.
WO2011019641A3 (en) Nutritional compositions including exogenous nucleotides
EP2539354A4 (en) FOR RANTES-CODING NUCLEIC ACID MOLECULES AND COMPOSITIONS AND METHODS THEREWITH