CN110818502A - Biological fertilizer for preventing and treating tobacco root black rot and preparation method thereof - Google Patents
Biological fertilizer for preventing and treating tobacco root black rot and preparation method thereof Download PDFInfo
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- CN110818502A CN110818502A CN201911040971.8A CN201911040971A CN110818502A CN 110818502 A CN110818502 A CN 110818502A CN 201911040971 A CN201911040971 A CN 201911040971A CN 110818502 A CN110818502 A CN 110818502A
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B1/00—Superphosphates, i.e. fertilisers produced by reacting rock or bone phosphates with sulfuric or phosphoric acid in such amounts and concentrations as to yield solid products directly
- C05B1/02—Superphosphates
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- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
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Abstract
The invention discloses a biological fertilizer for preventing and treating tobacco root black rot and a preparation method thereof, and belongs to the technical field of tobacco pest control, the biological fertilizer is composed of 26-30 parts of acer truncatum seed cake, 18-22 parts of nitrogen fertilizer, 13-20 parts of phosphate fertilizer, 16-22 parts of potassium fertilizer, 2-3 parts of mineral potassium fulvate, 1-2 parts of polyaspartic acid, 4-9 parts of sea snail shell powder, 0.5-2.5 parts of medium trace elements, 0.5-2 parts of chitosan, 0.5-1 part of agricultural algin, 0.5-1 part of glucose and 1-3 parts of compound microbial agent. The biological fertilizer is prepared by the processes of crushing, mixing and the like of the raw materials. The product has comprehensive nutrients, can meet the nutrient elements required by the growth of tobacco, has a better control effect on the black rot of tobacco roots, can reduce the application of chemical pesticides and chemical fertilizers after application, and has the effects of protecting soil and improving the quality of tobacco leaves.
Description
Technical Field
The invention belongs to the technical field of tobacco pest control, and particularly relates to a biological fertilizer for preventing and treating tobacco root black rot and a preparation method thereof.
Background
The black rot of tobacco root is a fungal disease harmful to tobacco, which is diseased all over the world, and can be infected by diseases in seedling stage and larger tobacco plant, after infection, the young stem, cotyledon and root tip of tobacco can be blackened and rotten, the root system of the serious disease plant is rotten and black, the tobacco plant grows slowly, becomes yellow and dwarfed, the leaf turns yellow and becomes thin, and the yield and quality of tobacco are seriously affected.
The etiology of the tobacco root black rot is moniliforme, which belongs to fungi of Deuteromycotina. The pathogenic bacteria can infect more than 30 plants of Solanaceae, Leguminosae, Cucurbitaceae and the like, and are mainly parasitic on disease residues, manure and bacteria-carrying soil, and the optimal disease attack temperature is 17-23 ℃. The disease is slight when the temperature is below 15 ℃ and above 26 ℃, and the disease is serious when the relative humidity is above 80%. The low temperature and heavy rain or continuous rainy days are easy to cause the popularity.
As an important economic crop in China, the occurrence of diseases of roots and stems of tobacco seriously affects the growth of the tobacco, the continuous cropping age of tobacco planting is prolonged, a large amount of chemical fertilizers are put into the tobacco, and the soil-borne diseases of the tobacco are in a trend of increasing in recent years, wherein the black rot of the tobacco roots is also a disease which seriously affects the tobacco production, and the harm of the black rot of the tobacco is also increased year by year. At present, the prevention and treatment of the black rot of the tobacco roots are generally medicinal prevention and treatment, and after the black rot of the tobacco roots is attacked, mancozeb, thiophanate methyl and prochloraz manganese are adopted for spraying treatment, so that the prevention and treatment effect is poor, and the environment is adversely affected.
Disclosure of Invention
The prior art has the problems that the prevention and the treatment of the tobacco root black rot are generally drug prevention and treatment, the prevention and the treatment effect are poor, and the environment is adversely affected.
In order to solve the problems, the technical scheme adopted by the invention is as follows:
a biofertilizer for preventing and treating tobacco root black rot comprises 26-30 parts of acer truncatum seed cake, 18-22 parts of nitrogen fertilizer, 13-20 parts of phosphate fertilizer, 16-22 parts of potash fertilizer, 2-3 parts of mineral potassium fulvate, 1-2 parts of polyaspartic acid, 4-9 parts of concha shell powder, 0.5-2.5 parts of medium trace elements, 0.5-2 parts of chitosan, 0.5-1 part of agricultural algin, 0.5-1 part of glucose and 1-3 parts of a compound microbial agent; the nitrogen fertilizer is any one of ammonium nitrate, nitrophosphate and nitramonphosphate; the phosphate fertilizer is any one of monoammonium phosphate, calcium magnesium phosphate or triple superphosphate; the potash fertilizer is potassium sulfate or potassium nitrate; the medium trace elements consist of magnesium sulfate, zinc sulfate, borax and ammonium molybdate.
The biological fertilizer for preventing and treating tobacco root black rot comprises a compound microbial agent and a biological agent, wherein the compound microbial agent comprises waxy bacillus powder, bacillus pumilus powder and streptomyces vernalis powder, the weight ratio of the waxy bacillus powder to the bacillus pumilus powder to the streptomyces vernalis powder is 1-2: 1-3, the number of viable bacterial colonies contained in each bacterial powder is not less than 108cfu/g。
The compound microbial agent of the biological fertilizer for preventing and treating tobacco root black rot is composed of paenibacillus polymyxa powder, bacillus subtilis powder, bacillus pumilus powder and bacillus thuringiensis powder, the weight ratio of the paenibacillus polymyxa powder, the bacillus subtilis powder, the bacillus pumilus powder and the bacillus thuringiensis powder is 1: 1-2: 1-3, the number of living bacterial colonies contained in each bacterial powder is not less than 108cfu/g。
The biological fertilizer for preventing and treating the tobacco root black rot comprises 0.3-2 parts of magnesium sulfate, 0.15-0.2 part of zinc sulfate, 0.04-0.25 part of borax and 0.01-0.05 part of ammonium molybdate.
The biological fertilizer for preventing and treating tobacco root black rot is prepared from 26 parts of acer truncatum seed cake, 22 parts of ammonium nitrate, 13 parts of monoammonium phosphate, 22 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 9 parts of sea snail shell powder, 0.3 part of magnesium sulfate, 0.15 part of zinc sulfate, 0.04 part of borax, 0.01 part of ammonium molybdate, 0.5 part of chitosan, 0.5 part of agricultural algin, 0.5 part of glucose, 0.25 part of bacillus cereus, 0.25 part of bacillus pumilus, 0.25 part of spring festival cake, and0.5 portion of streptomycete, and the number of living bacterial colonies contained in each bacterial powder is not less than 108cfu/g。
The biological fertilizer for preventing and treating tobacco root black rot is composed of 28 parts of acer truncatum seed cake, 18 parts of ammonium nitrate phosphate fertilizer, 20 parts of calcium magnesium phosphate fertilizer, 18 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 1 part of polyaspartic acid, 4 parts of conch shell powder, 1 part of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 1 part of bacillus cereus, 0.5 part of bacillus pumilus and 1 part of streptomyces vernalis, wherein the number of viable colonies contained in each bacterial powder is not less than 108cfu/g。
The biological fertilizer for preventing and treating tobacco root black rot is composed of 30 parts of acer truncatum seed cake, 20 parts of nitric phosphate fertilizer, 16 parts of triple superphosphate, 16 parts of potassium nitrate, 2 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 6 parts of concha shell powder, 2 parts of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 0.5 part of bacillus cereus, 0.5 part of bacillus pumilus and 0.5 part of streptomyces vernalis, wherein the number of living colonies contained in each bacterial powder is not less than 108cfu/g。
The acer truncatum seed cake is decomposed by EM (effective microorganisms) for 25-30 days.
According to the biological fertilizer for preventing and treating the tobacco root black rot, the conch shell powder is calcined at 700-900 ℃, and the fineness of the conch shell powder is 40-60 meshes.
The preparation method of the biological fertilizer for preventing and treating the tobacco root black rot comprises the following steps:
(1) fermenting acer truncatum seed cake pulp by using EM (effective microorganisms) strains for 25-30 days to prepare fermented acer truncatum seed cake pulp;
(2) calcining the conch shells at 700-900 ℃, and then crushing to 40-60 meshes;
(3) the fermented acer truncatum seed cake, a nitrogen fertilizer, a phosphate fertilizer, a potassium fertilizer, mineral potassium fulvate, polyaspartic acid, conch shell powder, medium trace elements, chitosan, agricultural algin, glucose and a compound microbial agent are stirred and mixed uniformly by adopting a mixing method process according to the parts by weight to obtain the acer truncatum seed cake.
Has the advantages that: compared with the prior art, the invention has the advantages that:
(1) the biological fertilizer disclosed by the invention is added with a large amount of nutrient elements and medium and trace elements, so that the nutrients are comprehensive, the resistance of tobacco plants is improved, and the occurrence of black rot of tobacco roots is reduced; the fertilizer is convenient to apply, can meet the nutrient elements required by the growth of tobacco, and can reduce the application of chemical pesticides and chemical fertilizers after being applied.
(2) The invention can obviously improve the quality of tobacco leaves by scientifically matching medium and trace nutrient elements, plant stimulin chitosan and agricultural algin.
(3) The soil can be improved and the cultivated land quality can be improved by adding the mineral source potassium fulvate and the sea snail shell powder.
(4) The added composite functional microorganism effectively improves the micro-ecological environment of the rhizosphere of the soil and reduces the occurrence of the black rot of the tobacco root.
Detailed Description
In order to make the aforementioned objects, features and advantages of the present invention comprehensible, embodiments accompanied with examples are described in detail below.
Bacillus cereus (Bacillus cereus), strain number: CGMCC 1.911, storage site: china general microbiological culture Collection center;
bacillus pumilus (Bacillus pumilus), strain number: CGMCC 1.4442, storage site: china general microbiological culture Collection center;
streptomyces kasugaensis (Streptomyces kasugaensis) strain number: ACCC 41813, storage site: china center for agricultural microbial strain preservation and management.
The preparation method of the bacillus cereus powder refers to the content disclosed in Bacillus dhs-330-021 powder preparation and stability research of the department of Shaoxing and the like, skim milk, trehalose, β -cyclodextrin and sodium glutamate are taken as protective agents, and spray drying (inlet temperature is 90 ℃, outlet temperature is 50 ℃, and injection speed is 2mL/min) is adopted to prepare the bacillus cereus powder.
The preparation method of the Bacillus pumilus powder refers to the content disclosed in Bacillus dhs-330-021 powder preparation and stability research of Skooguang et al, takes skim milk, trehalose, β -cyclodextrin and sodium glutamate as protective agents, and adopts spray drying (inlet temperature is 95 ℃, outlet temperature is 60 ℃, and injection speed is 4mL/min) to prepare the Bacillus pumilus powder.
The invention relates to a preparation method of streptomyces vernalis bacterial powder, which is called patent No. ZL2014105705704 and is named as an organic material decomposition agent rich in sodium alginate active substances and a preparation method of streptomyces albidoflaccida bacterial powder in the preparation method.
Example 1
A biofertilizer for preventing and treating tobacco root black rot is prepared by fermenting Acer truncatum seed cake with EM bacteria for 28 days to obtain fermented Acer truncatum seed cake; calcining conch shell at 700 deg.C, and pulverizing to 50 mesh; the following components in parts by weight: 26 parts of fermented acer truncatum seed cake meal, 22 parts of ammonium nitrate, 13 parts of monoammonium phosphate, 22 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 9 parts of concha shell powder, 0.3 part of magnesium sulfate, 0.15 part of zinc sulfate, 0.04 part of borax, 0.01 part of ammonium molybdate, 0.5 part of chitosan, 0.5 part of agricultural algin, 0.5 part of glucose, 0.25 part of bacillus cereus powder, 0.25 part of bacillus pumilus powder and 0.5 part of streptomyces vernalis powder, wherein the number of living colonies contained in each bacterium powder is not less than 108cfu/g, uniformly mixing through a mixing process of the organic-inorganic compound fertilizer, and obtaining a finished product after the finished product is qualified according to NY884-2012, wherein the dosage per mu is 100-125 kg and the finished product is used as a base fertilizer.
Example 2
A biofertilizer for preventing and treating tobacco root black rot is prepared by fermenting Acer truncatum seed cake with EM bacteria for 28 days to obtain fermented Acer truncatum seed cake; calcining conch shell at 900 deg.C, and pulverizing to 50 mesh; the weight portions are as follows: 28 parts of fermented acer truncatum seed cake meal, 18 parts of nitramine phosphate fertilizer, 20 parts of calcium magnesium phosphate fertilizer, 18 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 1 part of polyaspartic acid, 4 parts of conch shell powder, 1 part of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 1 part of bacillus cereus, 0.5 part of bacillus pumilus and 1 part of streptomyces vernalis, wherein each part isThe number of viable bacterial colonies contained in the bacterial powder is not less than 108cfu/g. Through the mixing process of the organic-inorganic compound fertilizer, after uniformly mixing, obtaining a finished product after being qualified according to NY884-2012 inspection, wherein the mu dosage is 100-125 kg and the finished product is used as a base fertilizer.
Example 3
A biofertilizer for preventing and treating tobacco root black rot is prepared by fermenting Acer truncatum seed cake with EM bacteria for 30 days to obtain fermented Acer truncatum seed cake; calcining conch shell at 800 deg.C, and pulverizing to 60 mesh; the weight portions are as follows: 30 parts of fermented acer truncatum seed cake meal, 20 parts of nitric phosphate fertilizer, 16 parts of triple superphosphate, 16 parts of potassium nitrate, 2 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 6 parts of conch shell powder, 2 parts of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 0.5 part of bacillus cereus, 0.5 part of bacillus pumilus and 0.5 part of streptomyces vernalis, wherein the number of living colonies contained in each bacterial powder is not less than 108cfu/g. Through a mixing process of the organic-inorganic compound fertilizer, after uniformly mixing, obtaining a finished product after being qualified according to NY884-2012, wherein the mu dosage is 100-125 kg and the finished product is used as a base fertilizer.
And (3) field test:
the test field is arranged in a Toyowa-east village along a wave and country in Yongfeng county in Jian city, and the time is 2-11 months in 2018. The tested soil is a sesame and sand mud field, and the basic physicochemical properties are as follows: 20.37g/kg of organic matter, 48.29mg/kg of alkaline hydrolysis nitrogen, 19.46mg/kg of available phosphorus, 79.15mg/kg of quick-acting potassium and 6.21 of pH value; the tobacco variety is Yunyan 87. The previous crop is rice, the biofertilizer prepared in the examples 1-3 is applied to the rice in a furrow application mode in 2018 for test treatment, the biofertilizer is applied to each mu according to 125kg, the fertilization mode of a control group is the same as that of a test group, and the applied biofertilizer is 60kg of a special tobacco compound fertilizer and 50kg of an organic fertilizer (the proportion of the special tobacco compound fertilizer is 10-8-20, a manufacturer is Yunnan leaf chemical fertilizer limited company, the organic fertilizer is a fermented acer truncatum seed cake fertilizer, and the manufacturer is the Yunnan leaf chemical fertilizer limited company).
The test is repeated for 3 times, the block is randomly designed, the block is arranged along the soil fertility gradient direction, 60 plants are planted in each cell, the block is set as a 3-row block, 20 plants are planted in each row, the cultivation specification is 1.2m multiplied by 0.5m, the tobacco leaves in each cell are collected singly, are put on a kang by weaving rods independently, are treated separately after being baked, and are weighed and recorded.
And (3) preparing soil and fertilizing the test group and the control group in the above way after 2 months and 15 days, transplanting after 3 months and 1 day, wherein the row spacing is 1.2m multiplied by 0.5m, performing test design when soil preparation and fertilization are performed, setting treatment every three rows, and making block groups and cell furrows. Other cultivation management is the same, such as: watering is carried out after the tobacco seedlings are transplanted, watering is carried out from one end of each tobacco ridge along the opened ditch with a water faucet, the watering amount is 8kg/m, when water is watered to the other end of each tobacco ridge, the watering is stopped, and other ditches are watered; and weeding is carried out regularly according to actual conditions (the growth conditions of the plots are different, so that the weeding time of the plots is not uniform, and the specific time cannot be recorded), and the tobacco seedlings need to be protected during weeding. In the agglomeration period of 4 months and 6 days, in the vigorous growth period of 4 months and 24 days, in the mature period of 5 months and 30 days, the disease condition of the tobacco root black rot of the whole field is investigated in 4 months and 5 days, and in 4 months and 15 days, respectively, the tobacco leaves are collected and baked in small areas and separately collected and baked in 6 months and 4 days, 6 months and 21 days, 7 months and 6 days, respectively weighed and recorded according to the treatment after the tobacco leaves are graded, and the yield of each level is calculated.
The test results are as follows:
1. prevention and control effect of different embodiments on tobacco root black rot
As can be seen from Table 1, the incidence and disease index of the fertilizer applied in the experimental examples are lower than those of the conventional fertilizer, which shows that the fertilizer has better control effect on the tobacco root black rot after the fertilizer is applied in the examples.
TABLE 1 prevention and control of tobacco root rot by biofertilizers of various examples
2. Influence of different embodiments on agronomic characters of tobacco leaves in flat top period
As can be seen from Table 2, the plant height, the number of effective leaves, the stem circumference, the pitch, the middle leaves and the upper leaves of the fertilizer applied in the experimental example were superior to those of the conventional fertilizer.
TABLE 2 influence of biofertilizers of different examples on agronomic traits of tobacco leaves in the plateau phase
3. Influence of different embodiments on economic traits of flue-cured tobacco
According to GB2635-1992, the primary flue-cured tobacco leaves are graded and counted, and as shown in the results of economic properties of flue-cured tobacco in Table 3, compared with conventional control, the fertilizer applied to the experimental examples has obvious effects of increasing yield and value of the tobacco, and the proportion of upper and middle tobacco is obviously improved.
TABLE 3 Effect of biofertilizers of different examples on economic traits of flue-cured tobacco
4. Effect of different examples on the intrinsic chemical composition of tobacco leaves
As can be seen from Table 4, after the biofertilizers of the examples are applied, total nitrogen, nicotine and chlorine are obviously reduced, and total sugar, reducing sugar and potassium are improved to a certain extent, which indicates that the quality of tobacco leaves is improved to a certain extent. The total sugar and reducing sugar are measured by YC/T159-2002; the nicotine is measured by YC/T160-2002; the total nitrogen is measured by YC/T161-2002; the chlorine is measured by YC/T162-2002; the potassium was measured using YC/T173-2003.
TABLE 4 Effect of biofertilizers of different examples on the intrinsic chemical composition of tobacco leaves
5. Effect of the different examples on the evaluation of tobacco Smoke quality (evaluation of tobacco Smoke quality YC/T138-1998)
TABLE 5 influence of biofertilizers of different examples on tobacco panel test
| Treatment of | Quality of fragrance | Amount of fragrance | Miscellaneous qi | Stiff head | Irritation property | Aftertaste | Total score |
| CK | 6.17 | 6.33 | 6.17 | 5.67 | 6.00 | 6.17 | 36.51 |
| Example 1 | 6.36 | 6.33 | 6.42 | 5.50 | 6.00 | 6.42 | 37.03 |
| Example 2 | 6.50 | 6.50 | 6.50 | 5.50 | 6.50 | 6.50 | 38.00 |
| Example 3 | 6.33 | 6.33 | 6.42 | 5.67 | 6.00 | 6.50 | 37.25 |
The tobacco leaves treated by the smoking evaluation and application examples have good aroma quality, sufficient aroma amount, good overall sensory quality and highest score.
The present invention has been described in terms of the above embodiments, and it should be understood that the above embodiments are not intended to limit the present invention in any way, and all technical solutions obtained by using equivalents or equivalent changes fall within the protection scope of the present invention.
Claims (10)
1. The bio-fertilizer for preventing and treating tobacco root black rot is characterized by comprising 26-30 parts of acer truncatum seed cake, 18-22 parts of nitrogen fertilizer, 13-20 parts of phosphate fertilizer, 16-22 parts of potassium fertilizer, 2-3 parts of mineral potassium fulvate, 1-2 parts of polyaspartic acid, 4-9 parts of sea snail shell powder, 0.5-2.5 parts of medium trace elements, 0.5-2 parts of chitosan, 0.5-1 part of agricultural algin, 0.5-1 part of glucose and 1-3 parts of a compound microbial agent; the nitrogen fertilizer is any one of ammonium nitrate, nitrophosphate and nitramonphosphate; the phosphate fertilizer is any one of monoammonium phosphate, calcium magnesium phosphate or triple superphosphate; the potash fertilizer is potassium sulfate or potassium nitrate; the medium trace elements consist of magnesium sulfate, zinc sulfate, borax and ammonium molybdate.
2. The biological fertilizer for preventing and treating tobacco root black rot according to claim 1, wherein the compound microbial agent consists of bacillus cereus powder, bacillus pumilus powder and streptomyces vernalis powder, the weight ratio of the bacillus cereus powder to the bacillus pumilus powder to the streptomyces vernalis powder is 1-2: 1-3, and the bacterial colony number of each bacterial powder is not less than 108cfu/g。
3. The biofertilizer for preventing and treating tobacco black rot according to claim 1, wherein the compound microbial agent comprises paenibacillus polymyxa powder, bacillus subtilis powder, bacillus pumilus powder and bacillus thuringiensis powder, the weight ratio of the paenibacillus polymyxa powder, the bacillus subtilis powder, the bacillus pumilus powder and the bacillus thuringiensis powder is 1: 1-2: 1-3, the number of living colonies contained in each bacterial powder is not less than 108cfu/g。
4. The biofertilizer for controlling tobacco root black rot according to claim 1, wherein said medium trace elements are composed of 0.3 to 2 parts of magnesium sulfate, 0.15 to 0.2 part of zinc sulfate, 0.04 to 0.25 part of borax, and 0.01 to 0.05 part of ammonium molybdate.
5. The biofertilizer for preventing and treating tobacco root black rot according to claim 1, which is composed of 26 parts of acer truncatum seed cake, 22 parts of ammonium nitrate, 13 parts of monoammonium phosphate, 22 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 9 parts of sea snail shell powder, 0.3 part of magnesium sulfate, 0.15 part of zinc sulfate, 0.04 part of borax, 0.01 part of ammonium molybdate, 0.5 part of chitosan, 0.5 part of agricultural algin, 0.5 part of glucose, 0.25 part of bacillus cereus powder, 0.25 part of bacillus pumilus powder and 0.5 part of streptomyces vernalis powder, wherein the number of viable colonies contained in each bacterial powder is not less than 108cfu/g。
6. The biofertilizer for controlling tobacco root black rot according to claim 1, characterized in thatCharacterized in that the fertilizer consists of 28 parts of acer truncatum seed cake dregs, 18 parts of ammonium nitrate phosphate fertilizer, 20 parts of calcium magnesium phosphate fertilizer, 18 parts of potassium sulfate, 3 parts of mineral potassium fulvate, 1 part of polyaspartic acid, 4 parts of conch shell powder, 1 part of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 1 part of bacillus cereus, 0.5 part of bacillus pumilus and 1 part of streptomyces vernalis, the number of living bacterial colonies contained in each bacterial powder is not less than 108cfu/g。
7. The biofertilizer for preventing and treating tobacco root black rot according to claim 1, which comprises 30 parts of acer truncatum seed cake, 20 parts of nitrophosphate fertilizer, 16 parts of triple superphosphate, 16 parts of potassium nitrate, 2 parts of mineral potassium fulvate, 2 parts of polyaspartic acid, 6 parts of sea snail shell powder, 2 parts of magnesium sulfate, 0.2 part of zinc sulfate, 0.25 part of borax, 0.05 part of ammonium molybdate, 2 parts of chitosan, 1 part of agricultural algin, 1 part of glucose, 0.5 part of bacillus cereus, 0.5 part of bacillus pumilus and 0.5 part of streptomyces vernalis, wherein the number of viable bacterial colonies contained in each bacterial powder is not less than 108cfu/g。
8. The biofertilizer for preventing and treating tobacco root black rot according to any one of claims 1 to 7, characterized in that acer truncatum seed cake is matured after being fermented for 25-30 days by EM (effective microorganisms).
9. The biofertilizer for preventing and treating tobacco root black rot according to any one of claims 1 to 7, characterized in that the sea snail shell powder is calcined at 900 ℃ and 700 ℃ and has fineness of 40-60 meshes.
10. A method of preparing a biofertilizer for controlling tobacco root black rot according to any one of claims 1 to 7, comprising the steps of:
(1) fermenting acer truncatum seed cake pulp by using EM (effective microorganisms) strains for 25-30 days to prepare fermented acer truncatum seed cake pulp;
(2) calcining the conch shells at 700-900 ℃, and then crushing to 40-60 meshes;
(3) the fermented acer truncatum seed cake, a nitrogen fertilizer, a phosphate fertilizer, a potassium fertilizer, mineral potassium fulvate, polyaspartic acid, conch shell powder, medium trace elements, chitosan, agricultural algin, glucose and a compound microbial agent are stirred and mixed uniformly by adopting a mixing method process according to the parts by weight to obtain the acer truncatum seed cake.
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| CN114455996A (en) * | 2022-01-17 | 2022-05-10 | 镇江贝思特有机活性肥料有限公司 | Special seaweed bio-fertilizer for tobacco and special fertilizer applying device |
| CN115024336A (en) * | 2022-05-18 | 2022-09-09 | 云南省农业科学院农业环境资源研究所 | Application of aspergillus hundred-year-old and/or liquid bio-organic fertilizer in prevention and treatment of tobacco diseases |
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| CN114455996A (en) * | 2022-01-17 | 2022-05-10 | 镇江贝思特有机活性肥料有限公司 | Special seaweed bio-fertilizer for tobacco and special fertilizer applying device |
| CN115024336A (en) * | 2022-05-18 | 2022-09-09 | 云南省农业科学院农业环境资源研究所 | Application of aspergillus hundred-year-old and/or liquid bio-organic fertilizer in prevention and treatment of tobacco diseases |
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