CN108484284A - A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof - Google Patents
A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof Download PDFInfo
- Publication number
- CN108484284A CN108484284A CN201810541033.5A CN201810541033A CN108484284A CN 108484284 A CN108484284 A CN 108484284A CN 201810541033 A CN201810541033 A CN 201810541033A CN 108484284 A CN108484284 A CN 108484284A
- Authority
- CN
- China
- Prior art keywords
- bacterial manure
- bacteria
- complex microorganism
- fermentation
- complex
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F3/00—Fertilisers from human or animal excrements, e.g. manure
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Pest Control & Pesticides (AREA)
- Biomedical Technology (AREA)
- Virology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Microbiology (AREA)
- Soil Sciences (AREA)
- Medicinal Chemistry (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a kind of phosphorus decomposings, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof.The bacterial manure is obtained by complex micro organism fungicide fermentation fowl and animal excrement, and the complex micro organism fungicide is mass ratio 35:2‑3:12 nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3.By the complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:20 25 ten thousand mixing, the aerobic composting fermentation 12 15 days at 40 DEG C 70 DEG C, then 40 45 days secondary naturally decomposed is carried out, obtain complex microorganism function bacterial manure.The complex microorganism function bacterial manure of the present invention is suitble to various cereal crops, industrial crops and fruit tree etc., it is particularly suitable for the industrial crops such as various melon dishes, because containing special functional flora, it can be while improving edaphon environment, kill various pathogenic bacteria, such as fungi, bacterium reduce the incidence of crop, and then improve crop yield and quality.
Description
Technical field
The present invention relates to microbial manure technical fields, and in particular to a kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterium
Fertilizer and preparation method thereof.
Background technology
Microbial manure refers to a kind of particular product agent containing active microorganism, is applied to that in agricultural production spy can be obtained
Fixed fertilizer effect.During the generation of this effect, active microorganism plays key effect.
After microbial manure is manured into soil, the inert element in air, soil is converted using the vital movement of microorganism
The ionic state element that can be directly absorbed for crop；The inorganic matter activation of indissoluble in soil is become into soluble inorganic matter, to increase
Add available N and P, the potassium content in soil, while the substance that crop cannot directly utilize from soil being converted to and being absorbed
The substance utilized, manufacture and assistance crops absorb nutrition, improve crop nutritional condition, and the activity of pathogen, enhancing is inhibited to make
Object is disease-resistant and drought-resistant ability.Organic Matter Transformation can be made to form humus in the activity of microorganism, form new soil group
Kernel structure is increase soil fertility, and improves soil physical and chemical property, enhancing soil fertilizer, water holding capacity, and then improve the yield of crop
And quality.
First, the quality of biological organic fertilizer quality depends primarily on strain itself and beneficial microorganism in bio-feritlizer
Viable count and its action intensity.China's agricultural production weak foundation, a variety of natures, ecological environment are severe, most areas by arid,
The influence of the factors such as soil physical and chemical property, microbial manure cause biological flora to reduce after being manured into soil, effect unobvious, institute
, heatproof excellent with selection and breeding, drought-resistant strain ensure that due viable count is that bio-organic fertilizer manufacturing enterprise overcomes in fertilizer
The problem of.
Secondly, it is most of containing microbial organic fertilizer be using organic matter as carrier, adsorb after microbial bacterial agent with it is organic and inorganic
The product that chemical fertilizer mixes.Common microbial strains non-refractory and saline and alkaline, general growth temperature is 20-40 DEG C, works as temperature
When reaching 60 DEG C or more, 80% microorganism will be dead, and temperature is higher, and the speed of microorganism death is faster.PH value is less than 6 or big
It is difficult survival in 8, seriously affects the effect of bacterial manure.Therefore, some drought-resistant, heatproofs of selection and breeding, saline-alkali tolerant effective strain very
It is important, especially screen the problem that some applicable bacillus are even more many enterprises pay attentions.
Third causes it to have a single function currently, microbial-bacterial fertilizer available on the market is mostly single culture, and effect is single,
To realize complex function, it is necessary to while or substep apply the function bacterial manure of different performance, so that producer's cost is increased, and each
Mutual phagocytosis between strain counteracts the effect of a certain function strain.Because having between beneficial rhizosphere microorganisms and crop relatively strong
Selectivity, also have certain requirement to environmental condition, thus screening, compounding complex function strain and reach mutual symbiosis, altogether
It deposits, realizes the compound series of products of all kinds of microbial inoculums, could meet the needs of different regions Different Crop.
4th, since last century the eighties, the lasting input of inorganic elements fertilizer has resulted in China's majority Soils In The Region
The enrichment of inorganic salts, as inorganic nitrogen accounts for full nitrogen ratio highest up to 40% in the soil；Phosphorus in soil generally with plant not
The indissoluble mineral form that can be utilized exists, and free state Phos level is substantially less than plant growth optimum level in soil.Equally,
The available potassium that potassium element 95% is present in potassium feldspar and mica, and can be absorbed and used by plants in soil only accounts for full potassium amount
2%.
Invention content
In view of the above problem, a kind of phosphorus decomposing of present invention offer, potassium fixed nitrogen complex microorganism function bacterial manure and its preparation side
Method, that improves bacterial manure uses resultant effect.
Technical scheme is as follows：
A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure are obtained, institute by complex micro organism fungicide fermentation fowl and animal excrement
It includes mass ratio 3-5 to state complex micro organism fungicide:2-3:Nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and the potassium solubilizing bacteria K3 of 1-2.
Preferably, in the complex micro organism fungicide, thalline total quantity be more than 4,700,000,000/gram.
Preferably, in the complex micro organism fungicide, the living bacteria count of nitrogen-fixing bacteria N1 is more than 2,000,000,000/gram, phosphate solubilizing bacteria FL7
Living bacteria count be more than 200,000,000/gram, the living bacteria count of potassium solubilizing bacteria K3 is more than 2,500,000,000/gram.
Preferably, the thalline total quantity of the complex microorganism function bacterial manure be more than 0.2 hundred million/gram.
Preferably, the weight ratio of the complex micro organism fungicide and the fowl and animal excrement is 1:20-25 ten thousand.
The present invention also provides the preparation method of complex microorganism function bacterial manure described in above-mentioned any one technical solution, packets
Include following steps：
(1) by nitrogen-fixing bacteria N1 strains, phosphate solubilizing bacteria FL7 strains and potassium solubilizing bacteria K3 strains respectively in respective fermentation medium
Fermentation terminates fermentation as the spore forming rate Da Dao≤80% of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 or potassium solubilizing bacteria K3 in zymotic fluid；
According to the mass ratio 3-5 of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3:2-3:1-2 batch mixings, obtain complex micro organism fungicide；
(2) by the complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:20-25 ten thousand is mixed, at 40 DEG C -70 DEG C
Lower aerobic composting fermentation 12-15 days obtains just fermented manure；
(3) the just fermented manure is subjected to 40-45 days secondary naturally decomposed, obtains complex microorganism function bacterial manure.
Preferably, the fermentation medium of the nitrogen-fixing bacteria N1 by weight percentage, including mannitol 0.5-1.5%, phosphorus
Acid dihydride potassium 0.005-0.03%, magnesium sulfate 0.005-0.03%, sodium chloride 0.005-0.03%, calcium sulfate 0.005-
0.02%, calcium carbonate 0.3-0.7%, agar 1.0-3.0%, remaining is water；The pH value of the nitrogen-fixing bacteria N1 fermentation mediums is
6.5-7.0。
Preferably, the fermentation medium of the phosphate solubilizing bacteria FL7 by weight percentage, including sulfuric acid diamino 0.03-
0.06%, potassium chloride 0.001-0.004%, sodium chloride 0.001-0.004%, green-vitriol 0.0001-0.0004%, a water
Manganese sulfate 0.0001-0.0004%, epsom salt 0.001-0.004%, glucose 0.5-1.5%, yeast powder 0.03-
0.07%, calcium phosphate 0.3-0.6%, agar 1.0-3.0%, remaining is water；The pH value of the phosphate solubilizing bacteria FL7 fermentation mediums is
7-7.5。
Preferably, the fermentation medium of the potassium solubilizing bacteria K3 by weight percentage, including disodium hydrogen phosphate 0.15-
0.25%, trichlorine iron 0.0004-0.0006%, epsom salt 0.04-0.06%, calcium carbonate 0.005-0.015%, sucrose
0.2-0.4%, feldspar in powder 0.05-0.15%, remaining is water；The pH value of the potassium solubilizing bacteria K3 fermentation mediums is 7.0-7.5.
Preferably, it is of the present invention it is secondary it is naturally decomposed after further include screening：By the secondary naturally decomposed material sieve
Point；The obtained rough material of sieving is mixed with fowl and animal excrement, is fermented again by complex micro organism fungicide, is made complex microorganism
Function bacterial manure.
Compared with prior art, the present invention has the following advantages：
Phosphorus decomposing of the present invention, potassium fixed nitrogen complex microorganism function bacterial manure, by complex micro organism fungicide aerobic fermentation fowl and animal excrement
It obtains, the complex micro organism fungicide includes mass ratio 3~5:2~3:1~2 nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria
K3.The tolerance that three kinds of bacterial strains have had the growth conditions such as high temperature, high salt concentration, high ammonium concentration and slight alkali environment
Can, various soil environments are can adapt to, to play its phosphorus decomposing, potassium decomposing and the function of fixed nitrogen, and improves rhizosphere environment, carries
High bacterial manure resultant effect.
The complex microorganism function bacterial manure of the present invention has the effect that effect and feature：
(1) improve crop rhizosphere niche
Synthesis or decomposition in three kinds of microbial life activities through the invention can improve due to chronic administration chemistry
The situation of soil hardening, physics and chemistry difference that fertilizer is brought makes crop rhizosphere soils soil moisture conservation, fertilizer conservation, and permeability is good, crop root
Prosperity, drought-resistance ability enhancing.Simultaneously as being formed beneficial to dominant colony group, cause of the energy antagonism with respect to weak tendency in crop rhizosphere
Pathogen is eliminated or weakened to germ population, to which crop morbidity be alleviated or avoided.
(2) with the obvious advantage on improving crop quality
The complex microorganism function bacterial manure of the present invention ferments to obtain with fowl and animal excrement, and the bacterial manure after fermentation has balanced nutrients
The effect of the relation between supply and demand, for improving the with obvious effects of quality of agricultural product.The exterior quality of agricultural product can not only be improved, improved
Storage tolerance performance, and optimize flavor taste, increase dry-matter accumulation.
(3) improve the quality of soil
The complex microorganism function bacterial manure of the present invention passes through the work(such as the fixed nitrogen of three kinds of specified microorganisms populations, phosphorus decomposing, potassium decomposing
On the one hand energy and microorganism can promote absorption, the life of another aspect microbial population to the growth-promoting functions of crop root
Activity can form a large amount of humus, improve soil physical chemistry performance, avoid a large amount of fixed and loss of chemical fertilizer in the soil, from
And improve fertilizer efficiency.Simultaneously as the decomposition of microorganism, synthesis (such as fixed nitrogen) effect, can improve primary soil to rhizosphere
For fertilizer amount, fertilizer amount is reduced, to Synergistic and energy-saving.
(4) production cost is low
Complex microorganism function bacterial manure of the present invention is compared with chemical fertilizer, and the consumed energy is few when production, and production cost is low.And
And due to the use of microbial manure, the utilization rate that can improve chemical fertilizer is at least up to 50% or more, has saved agriculture production cost.
(5) improve environment
The primary raw material of complex microorganism function bacterial manure of the present invention is feces of livestock and poultry, and microorganism is made by microbial fermentation
Organic multiple, mixed fertilizer, realizes waste utilization, can supply the fertilizer of agriculture low cost.Pass through point of microbial manure simultaneously
Solution, synthesis reduce the levels of toxic substances such as Air Hydrogen Sulfide, nitrogen dioxide gas, realize the deodorizing process of farm,
The other harmful substances matter such as heavy metal particles and nitrate in degradation or conversion soil is that green agriculture (ecology, organic agriculture) is first
The main source of manure of choosing.
In conclusion phosphorus decomposing of the present invention, potassium fixed nitrogen complex microorganism organic fertilizer, with three kinds of specified microorganisms to fowl and animal excrement
It carries out fermentation to be made, is suitble to various cereal crops, industrial crops and fruit tree etc., be particularly suitable for the industrial crops such as various melon dishes, because
Containing special functional flora, various pathogenic bacteria, such as fungi, bacterium can be killed while improving edaphon environment,
The incidence of crop is reduced, and then improves crop yield and quality.
Description of the drawings
Fig. 1 be phosphorus decomposing of the present invention, potassium fixed nitrogen complex microorganism organic fertilizer production process；
Fig. 2 is the flow chart that nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 zymotic fluids are prepared in embodiment 1.
Specific implementation mode
The present invention provides a kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure, and the bacterial manure is by complex micro organism fungicide
Fermentation fowl and animal excrement obtains, and the complex micro organism fungicide includes mass ratio 3-5:2-3:Nitrogen-fixing bacteria N1, the phosphate solubilizing bacteria FL7 of 1-2
With potassium solubilizing bacteria K3.
In the present invention, nitrogen-fixing bacteria N1 amount of nitrogen fixation is 1.81 × 10-2Mol/L, can 50 DEG C, 11% sodium chloride, 0.8mol/L
Ammonium ion, pH value are grown under the conditions ofs 4-10 etc..Phosphate solubilizing bacteria FL7 is phosphorus decomposing saccharomycete (pichia farinose), and phosphorus decomposing amount reaches
436.63 μ g/ml can be grown under the conditions ofs 45 DEG C, 23% sodium chloride, 6mol/L ammonium ions, pH value are 9 etc., and being one kind can incite somebody to action
The phosphorus that plant is difficult to absorb is converted into the microorganism of availability status, in addition to can with activating soil in other than insoluble phosphorus, can be with
By influencing the type and quantity of secretions from plant roots, to increase the absorption to surrounding plants nutrient, plant is enable to fit
The environment of phosphorus should be lacked.Potassium solubilizing bacteria K3, potassium decomposing amount reach 4.10mg/L, can 55 DEG C, 14% sodium chloride, 1.2mol/L ammonium ions, pH
Value is grown under the conditions ofs 5-9 etc..It can be seen that above-mentioned three kinds of bacterial strains are to high temperature, high salt concentration, high ammonium concentration and meta-alkalescence
The tolerance performance that the growth conditions such as environment have had, can adapt to various soil environments, play respective function.
Complex micro organism fungicide of the present invention is preferably by nitrogen-fixing bacteria N1 strains, phosphate solubilizing bacteria FL7 strains and potassium solubilizing bacteria K3 bacterium
Kind is fermented in the medium respectively, and the zymotic fluid after fermentation is according to mass ratio 3-5:2-3:The ratio batch mixing of 1-2 is prepared.This
Invention does not limit the source of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3, can be bought from depositary institution.Institute of the present invention
The fermentation process for stating nitrogen-fixing bacteria N1 strains, phosphate solubilizing bacteria FL7 strains and potassium solubilizing bacteria K3 strains preferably includes actication of culture, shaking flask training
It supports, the process of seeding tank fermentation and ferment tank.Specifically fermentation process can be according to the common fermentation processes in this field
It can.Fermentation temperature is preferably controlled in 25~35 DEG C, more preferably 28~32 DEG C.Stirring in fermentation process in fermentation tank is preferably adopted
It is carried out with frequency conversion.As the spore forming rate Da Dao≤80% of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 or potassium solubilizing bacteria K3 in each zymotic fluid,
The fermentation for terminating above-mentioned three kinds of microorganisms obtains the zymotic fluid containing nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 respectively, presses
Ratio is mixed to get complex micro organism fungicide.
In the present invention, the fermentation medium of nitrogen-fixing bacteria N1 by weight percentage, preferably includes mannitol 0.5-1.5%,
Potassium dihydrogen phosphate 0.005-0.03%, magnesium sulfate 0.005-0.03%, sodium chloride 0.005-0.03%, calcium sulfate 0.005-
0.02%, calcium carbonate 0.3-0.7%, agar 1.0-3.0%, remaining is water；More preferably include mannitol 0.8-1%, di(2-ethylhexyl)phosphate
Hydrogen potassium 0.015-0.02%, magnesium sulfate 0.015-0.02%, sodium chloride 0.015-0.02%, calcium sulfate 0.008-0.01%, carbon
Sour calcium 0.45-0.5%, agar 1.8-2.2%, remaining is water；The pH value of the nitrogen-fixing bacteria N1 fermentation mediums is preferably 6.5-
7.0, more preferably 6.8-6.9.
In the present invention, the fermentation medium of phosphate solubilizing bacteria FL7 by weight percentage, preferably includes sulfuric acid diamino 0.03-
0.06%, potassium chloride 0.001-0.004%, sodium chloride 0.001-0.004%, green-vitriol 0.0001-0.0004%, a water
Manganese sulfate 0.0001-0.0004%, epsom salt 0.001-0.004%, glucose 0.5-1.5%, yeast powder 0.03-
0.07%, calcium phosphate 0.3-0.6%, agar 1.0-3.0%, remaining is water；More preferably include sulfuric acid diamino 0.045-0.05%,
Potassium chloride 0.0025-0.003%, sodium chloride 0.0025-0.003%, green-vitriol 0.00025-0.0003%, sulfuric acid monohydrate
Manganese 0.00025-0.0003%, epsom salt 0.0025-0.003%, glucose 0.8-1%, yeast powder 0.045-0.05%,
Calcium phosphate 0.45-0.5%, agar 1.8-2%, remaining is water；The pH value of the phosphate solubilizing bacteria FL7 fermentation mediums is preferably 7-
7.5, more preferably 7.2~7.4.
In the present invention, the fermentation medium of potassium solubilizing bacteria K3 by weight percentage, preferably includes disodium hydrogen phosphate 0.15-
0.25%, trichlorine iron 0.0004-0.0006%, epsom salt 0.04-0.06%, calcium carbonate 0.005-0.015%, sucrose
0.2-0.4%, feldspar in powder 0.05-0.15%, remaining is water；More preferably include disodium hydrogen phosphate 0.18-0.2%, trichlorine iron
0.00048-0.0005%, epsom salt 0.048-0.05%, calcium carbonate 0.008-0.01%, sucrose 0.28-0.3%, potassium
Feldspar powder 0.08-0.1%, remaining is water；The pH value of the potassium solubilizing bacteria K3 fermentation mediums is preferably 7.0-7.5, more preferably
7.2~7.4.
In the complex micro organism fungicide that the present invention is prepared, microbial cells total amount be more than 4,700,000,000/gram, wherein fixed nitrogen
The living bacteria count of bacterium N1 is more than 2,000,000,000/gram, the living bacteria count of phosphate solubilizing bacteria FL7 is more than 200,000,000/gram, effective work of potassium solubilizing bacteria K3
Bacterium number be more than 2,500,000,000/gram.
The present invention also provides the preparation methods of complex microorganism function bacterial manure described in above-mentioned technical proposal, including following step
Suddenly：
(1) nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 are fermented in respective fermentation medium respectively, works as fermentation
In liquid when the spore forming rate Da Dao≤80% of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 or potassium solubilizing bacteria K3, terminate fermentation；According to nitrogen-fixing bacteria
The mass ratio 3-5 of N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3:2-3:1-2 batch mixings, obtain complex micro organism fungicide；
(2) by the complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:20-25 ten thousand is mixed, at 40 DEG C -70 DEG C
Lower aerobic composting fermentation 12-15 days obtains just fermented manure；
(3) the just fermented manure is subjected to 40-45 days secondary naturally decomposed, obtains complex microorganism function bacterial manure.
In the present invention, the preparation method of the complex micro organism fungicide referring to above-mentioned technical proposal record, herein not
It repeats again.
After obtaining complex micro organism fungicide, the present invention is by the complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:
20~250,000 mixing, aerobic composting fermentation 12~15 days at 40~70 DEG C obtain just fermented manure.The present invention is preferably described multiple
The weight ratio for closing microbial bacterial agent and fowl and animal excrement is preferably 1:22-24 ten thousand.The temperature of the preferred aerobic composting fermentation of the present invention is
40 DEG C -50 DEG C, fermentation time is preferably 14 days.The present invention is just fermented to be carried out at 70 DEG C or less, optional when temperature is higher than 70 DEG C
It carries out turning over throwing using turner, material is prevented to be carbonized under the conditions of excessive temperature, reduce the quality and content of organic matter, together
When also ensure survival volume and reproduction speed, the breeding amount of microorganism in access microbial inoculum, kill miscellaneous bacteria, pathogen, grass-seed etc..This
The concrete operations for inventing the compost use the conventional practices of those skilled in the art, turning interval time and turning number
The temperature of the visual fermentation materials of those skilled in the art is rationally adjusted.
The present invention is secondary naturally decomposed by the progress of first fermented manure, obtains complex microorganism function bacterial manure.It is secondary naturally rotten
Ripe that material is made to be cured again, material fermentation when preventing from just fermenting is incomplete, ferments in the soil after leading to field application
Local temperature occurs to increase, generates the gases such as ammonia, hydrogen sulfide and harms the crops.The present invention is preferably by the discharging of first fermented manure to certainly
Right decomposed field, stacks fermentation naturally.It is preferred that the side 150-200 just ferments, compost heap is at a bar stack, and preferably heap is 3-4 meters high, bottom
It is 4-5 meters wide.Naturally the time for stacking fermentation is 40~45 days, preferably 42~44 days.
Optionally, the present invention by it is secondary it is naturally decomposed after compost sieve, by the crop material mixed in waste with
And be not easy to be fermented the hard plant epidermis of decomposition and sift out, ensure to improve granulating rate in granulation process, that sifts out is completely not rotten
The plant epidermis of solution can return to fermenting procedure and carry out fermentation decomposition again.The present invention does not have special limit to the concrete operations of screening
It is fixed, using the conventional method for sieving in this field.In the specific embodiment of the invention, it is preferred to use screening machine is sieved
Point, remove the material of incomplete decomposition.The aperture of screening preferably uses 80-120 mesh nets to shine, and the rough material on sieve top is optional
It mixes with fowl and animal excrement, is fermented again by complex micro organism fungicide, make complex microorganism function bacterial manure.
Complex microorganism function bacterial manure of the present invention is optional to be granulated, and finished product is obtained.Prilling process uses this field
In conventional method, as comminutor be granulated.
In complex microorganism function bacterial manure of the present invention, the thalline total quantity of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 are excellent
Choosing be more than 0.2 hundred million/gram.
Complex microorganism function bacterial manure of the present invention is suitble to various cereal crops, industrial crops and fruit tree etc., is particularly suitable for each
The industrial crops such as kind melon dish.Complex microorganism function bacterial manure of the present invention is applied on cereal crops generally rests in 100-150 public affairs
Pound/acre, the industrial crops such as cotton rest in 150-200 kgs/acre, and the industrial crops such as melon dish rest in 150-300 kgs/acre,
The commodity trees such as fruit tree rest in 500-600 kgs/acre.
To make the objectives, technical solutions, and advantages of the present invention clearer, with reference to embodiment to the present invention into
Row detailed description, but they cannot be interpreted as limiting the scope of the present invention.
Embodiment 1
The preparation of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 zymotic fluids
1. the strain in freezing pipe is activated 22-26 hours in 35-37 DEG C of insulating box first, then according to following
Formula prepares the fermentation medium of each strain：
The allotment of nitrogen-fixing bacteria N1 fermentation mediums：By weight percentage：Mannitol 1%, potassium dihydrogen phosphate 0.02%, sulfuric acid
Magnesium 0.018%, sodium chloride 0.018%, calcium sulfate 0.01%, calcium carbonate 0.45%, agar 2.0%, remaining is made of sterile water.
The pH value of culture medium is adjusted to 6.8, culture medium 120 DEG C of high-temperature sterilizations 20 minutes before microbial inoculum feeds intake.
The allotment of phosphate solubilizing bacteria FL7 fermentation mediums：By weight percentage：Sulfuric acid diamino 0.045%, potassium chloride 0.003%,
Sodium chloride 0.003%, green-vitriol 0.0003%, manganese sulfate monohydrate 0.0003%, epsom salt 0.003%, glucose
1%, yeast powder 0.05%, calcium phosphate 0.5%, agar 2%, remaining is made of sterile water.The pH value for adjusting culture medium is 7.2,
Culture medium 120 DEG C of high-temperature sterilizations 20 minutes before microbial inoculum feeds intake.
The allotment of potassium solubilizing bacteria K3 culture mediums：By weight percentage：Disodium hydrogen phosphate 0.2%, trichlorine iron 0.0005%, seven water
Magnesium sulfate 0.05%, calcium carbonate 0.01%, sucrose 0.3%, feldspar in powder 0.1%, remaining is made of sterile water, adjusts culture medium
PH value 7.0-7.5, culture medium 120 DEG C of high-temperature sterilizations 20 minutes before microbial inoculum feeds intake.
After being inoculated with strain on the tablet of each culture medium, purifying 22-26 hours is carried out in 35-37 DEG C of insulating box, finally
Obtain plane strain or strain plane.
2. shaking flask cultivation stage:
A, the strain of a ring after purification, access is taken to be equipped in 250 milliliters of triangular flasks of 20 milliliters of seed culture mediums, then
It is placed in 35-37 DEG C of insulating box and shakes culture 3-5 hours, stationary culture 19-21 hours using 180 beats/min of shaking tables of frequency.
B, above-mentioned seed liquor is divided into 5 equal portions, accesses five 250 milliliters of triangular flasks for filling 20 milliliters of each fermentation mediums
In.It is again placed in 35-37 DEG C of insulating box and utilizes shake culture 22-26 hours in 180 beats/min of shaking tables of frequency.Then to kind
Sub- tank inoculation.
3. seeding tank fermentation stage
It is inoculated with from shaking flask strain to first class seed pot, inoculum concentration is controlled the 5.0% of the practical charging quality of first class seed pot；
Fermentation temperature is controlled at 30 DEG C, the total charge of fermentation medium controls the 60-70% in seed tank volume.Speed of agitator controls
90 revs/min, 180 revs/min of frequency conversion stirrings, it is 10 minutes that frequency conversion, which adjusts frequency time,.
The concrete technology operation of secondary seed tank is identical with first class seed pot with state modulator.
4. the ferment tank stage
Fermentation tank liquid amount is the 60%-75% of tank volume；Inoculum concentration is the 1%-5% of practical liquid amount；Fermentation temperature
Degree control is at 30 DEG C；Zymotic fluid pH in fermentation tank is controlled within the scope of the pH of each strain fermentation culture medium；Speed of agitator is with 100
Rev/min, 200 revs/min of frequency conversions carry out, rotating speed is too fast to generate destruction to mycelium, and rotating speed be also easy to produce barm slowly excessively,
And the growth and breeding of microorganism can be influenced because of dissolving hypoxgia.
As thalline spore forming rate Da Dao≤80%, fermentation is completed.It is spare to be then introduced into each fermentation liquid storage tank.
Embodiment 2
Nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and the potassium solubilizing bacteria K3 zymotic fluids that embodiment 1 is prepared are according to mass ratio 3:2:1
Ratio batch mixing, obtain complex micro organism fungicide；
By complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:250000 mixing, the aerobic composting fermentation at 40 DEG C.
Single slot size 75m × 4m × 1.5m of compost, the high 1m of material heap, single turning distance 3m.Stage aeration supplies oxygen need according to material
Control the blow rate required；Daily by material turning, each turning material moves backward 3 meters.Aerobic fermentation was completed by 14 days or so.
The microbial organic fertilizer raw material discharging containing complex microbial community that will be formed by 14 days or so aerobic fermentations
To naturally decomposed field, (general 180 side piles a bar stack, heap after stacking completion in 45 days or so is naturally decomposed for the second time naturally
It is 3 meters high, 5 meters of bottom width), sieved using screening machine, after granulation, form the qualified microbial organic fertilizer production containing complex micro organism fungicide
Product.
Embodiment 3
Nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and the potassium solubilizing bacteria K3 zymotic fluids that embodiment 1 is prepared are according to mass ratio 5:3:2
Ratio batch mixing, obtain complex micro organism fungicide；
By complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:200000 mixing, the aerobic composting fermentation at 50 DEG C.
Single slot size 75m × 4m × 1.5m of compost, the high 1m of material heap, single turning distance 3m.Stage aeration supplies oxygen need according to material
Control the blow rate required；Daily by material turning, each turning material moves backward 3 meters.Aerobic fermentation was completed by 13 days or so.
By the microbial organic fertilizer raw material discharging containing complex microbial community formed by aerobic fermentation to natural corruption
Ripe field stacks and completes within 40 days second naturally decomposed rear (150 sides pile a bar stack, and heap is 3 meters high, 4 meters of bottom width), profit naturally
It is sieved with screening machine, after granulation, forms the qualified microbial organic fertilizer product containing complex micro organism fungicide.
Embodiment 4
Nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and the potassium solubilizing bacteria K3 zymotic fluids that embodiment 1 is prepared are according to mass ratio 4:2:1
Ratio batch mixing, obtain complex micro organism fungicide；
By complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:220000 mixing, the aerobic composting fermentation at 60 DEG C.
Single slot size 75m × 4m × 1.5m of compost, the high 1m of material heap, single turning distance 3m.Stage aeration supplies oxygen need according to material
Control the blow rate required；Daily by material turning, each turning material moves backward 3 meters.Aerobic fermentation was completed by 12 days or so.
By the microbial organic fertilizer raw material discharging containing complex microbial community formed by aerobic fermentation to natural corruption
Ripe field, (general 200 side piles a bar stack, and heap is 4 meters high, bottom width 5 after stacking completion in 42 days is naturally decomposed for the second time naturally
Rice), it is sieved using screening machine, after granulation, forms the qualified microbial organic fertilizer product containing complex micro organism fungicide.
After testing, in the complex micro organism fungicide that the present invention is prepared, microbial cells total amount be more than 4,700,000,000/gram,
In, the living bacteria count of nitrogen-fixing bacteria N1 is more than 2,000,000,000/gram, the living bacteria count of phosphate solubilizing bacteria FL7 is more than 200,000,000/gram, potassium solubilizing bacteria K3's
Living bacteria count be more than 2,500,000,000/gram.
In the complex microorganism function bacterial manure that the present invention is prepared, nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 bacterium
Body total quantity be more than 0.2 hundred million/gram.
Embodiment 5
Cucumber application complex microorganism function bacterial manure application effect of the present invention is tested
Crop varieties：Cucumber, Tianjin spring No. four.
(1) conventional fertilizer application：Base fertilizer Common compound fertilizer (N, P2O5、K2Each 15%) 80 kgs/acre of O, mid-term top dressing 2 times, every time
15 kilograms of urea.
(2) experiment fertilizer：Base fertilizer complex microorganism function bacterial manure (N, P of the present invention2O5、K2O >=5%, function bacterium sum >=
0.2 hundred million/gram) 150 kgs/acre.
(3) experimental design：It repeats three times.
(4) cucumber was sowed April 20, transplanting on May 23.Start to adopt melon at the beginning of 6 months, other field management weedings, watering
Unanimously.
(5) test result：
Growth period is observed：The growth of conventional fertilizer application is normal；Neatly healthy and strong, the vines using the then emergence of microbial function bacterial manure
Sturdy, blade is plump, and percentage of fertile fruit is high, and no disease and pests harm, sugar-preserved gourd is straight, and curcumbitate is good, in good taste.
Yield comparison：The use of the cucumber per mu yield of complex microorganism function bacterial manure of the present invention is 6718 kilograms, compares conventional fertilizer application
(5298 kilograms of per mu yield) increases production 1420 kilograms, and rate of growth reaches 26.8%.
Disease survey also illustrates that the diseased plant rate using microbial function bacterial manure ecology composite fertilizer is extremely low, only accounts for 0.62%, and
And there is no grave illness strain, reduce 7.88% than conventional fertilizer application diseased plant rate.
Embodiment 6
Chinese cabbage complex microorganism function bacterial manure application effect of the present invention
Crop varieties：Chinese cabbage, Beijing three.
Application site：What village village of Yutian County of Hebei province towns Liang Jiadian
(1) control fertilizer：Microorganism Bacillus subtilis microbial inoculum (living bacteria count >=5.0 hundred million/gram) 2 kilograms per acre+have
Machine 150 kilograms of bottom application of fertilizer.
(2) experiment fertilizer：Complex microorganism function bacterial manure (N, P of the present invention2O5、K2O >=5%, function bacterium sum >=0.2
Hundred million/gram) 150 kilograms of base fertilizer per acre.
(3) experimental design：It repeats three times.
(4) August 7 is sowed, and such as field management weeding, intertillage, watering are consistent for other.
(5) test result：
Growth period is observed：Emergence using microbial function bacterial manure is neatly healthy and strong, and growing way is preferable, compares microbial inoculum+organic fertilizer
Growth is normal.
Yield comparison：Chinese cabbage per mu yield using microbial function bacterial manure is 6512 kilograms, than compareing microbial inoculum+organic fertilizer (mu
6128 kilograms of production) 384 kilograms of volume increase, rate of growth reaches 6.27%.
Disease survey also illustrates that the diseased plant rate using microbial function bacterial manure ecology composite fertilizer is extremely low, only accounts for 0.02%, and
And there is no grave illness strain, reduce 8.12% than control microbial inoculum+organic fertilizer diseased plant rate.
Embodiment 7
Husky white melon application complex microorganism function bacterium fertilizer efficiency fruit
Application site：Behind the Hebei province towns Leting County Xin Zhai orchid stick together village history person of outstanding talent at
Planting patterns：Brooder takes up an area 0.8 mu per canopy, and control family is adjacent same greenhouse.Area, water source, soil property,
Administrative skill and cultivation melon time consistency only carry out comparison application in the case of one difference of fertilising, as follows：
Canopy demonstrate per 300 kilograms of microbial function bacterial manure of canopy, control canopy is per the canopy green limited public affairs of health biotechnology of middle peasant
Department's production anti-2 kilograms of continuous cropping microbial bacterial agent of part matches 300 kilograms of organic fertilizer, the gold of Dezhou bio tech ltd production
One bag of full field (effective viable bacteria 200,000,000/gram) (5Kg fills water-soluble bacterial manure).
Method of administration is as follows：
1, demonstration canopy 300 kilograms of complex microorganism function bacterial manure of the present invention is spread on earth's surface, then turns over, bedding, covering ground
Transplanting membrane.
2, control canopy is ploughed after 300 kilograms and 2 kilograms anti-continuous cropping microbial ecological bacterial manure of farm manure are first spread fertilizer over the fields field, is covered
Transplanting membrane.The full field bacterial manure of gold carried out proposing seedling use for seedling stage.
Both the above method, upper roughly the same in input, it is irregular to compare melon seedling growing way in the canopy of canopy, continuous application two
The secondary full field bacterial manure of gold carries seedling, and melon seedling has mended one batch of another stubble.Substantial contribution is not only wasted, increases countryside tax system, more sternly
Weight is to delay farming season in greenhouse, makes to be that the white melon of sand of off-season cultivation loses anti-season advantage, melon evening city ten originally
Five to two ten days, using the demonstration canopy of microbial function bacterial manure than control per more than 3,000 member of income more than canopy, economic benefit was than control
Increase 80% or more.
Embodiment 8
Corn applies complex microorganism function bacterium fertilizer efficiency fruit of the present invention
Experiment sets 2 processing altogether：1,150 kilograms of complex microorganism function bacterial manure (demonstration) of the present invention be per acre；2, conventional to apply
Fertile (45% 75 kilograms of three element compound fertilizer is per acre)+mu biological phosphorus potassium bacterium (500,000,000/ml, the limited public affairs of Hebei Ji microbial technique
Department) 250ml seed dressings (control).0.1 mu of plot area, random alignment, in triplicate, experiment take up an area 1 mu altogether.
Testing site is located at the villages Dong great Quan of the townshiies Guo Jiatun of Yutian County, is calabash shell serving as a dipper jade 13 for examination corn variety, corn is broadcast on the 20th June
Kind, in the typhon mouth phase, respectively processing imposes urea by 15 kilograms per acre, and the agronomic measures such as watering, intertillage are identical.October 2 is by each
Processing repeats singles and singly receives.
As a result with analysis
1 complex microorganism function bacterial manure processing emergence of the present invention of processing is more neat, and 10 days or so after emergence, leaf color is dark green,
Robust growth, aerial root is flourishing after taking out hero.1,2 are shown in Table to the field investigation, sampling, measurement result in experimental plot.
The influence application form of 1 different disposal corn yield of table and economic characters
2 yield factors of table carry out significance analysis
T test Analysis is carried out to yield：Calculate SD=5.3454, t=8.734；Through t0.01=4.604, t0.05=
2.776, t values are significantly greater than t0.01, t0.05 respectively, it is seen that there are notable differences between processing.Apply complex microorganism function
Bacterial manure can increase production, and volume variance is extremely notable.
The above test results show that corn application complex microorganism function bacterial manure of the present invention has significant effect of increasing production,
This empirical average mu increases production 46.69 kilograms of corn, amount of increase in production 10.5%.Its reason of yield increased is mainly manifested in using composite microbial
Object function bacterial manure can promote corn ear to break up, and increase tassel row number and grain number per spike, mass of 1000 kernel be improved, using complex microorganism function
Bacterial manure is averaged tassel row number as 16.2 rows, relatively compares biological phosphorus potassium bacterium 15.9 rows of seed dressing and increases 0.3 row, grain number per spike average out to
It 451.07, relatively compares biological phosphorus potassium bacterium seed dressing 421.5 and increases 29.57.Mass of 1000 kernel increases by 10.23 grams.
Embodiment 9
Influence of the complex microorganism function bacterial manure of the present invention to corn yield, quality
Kind：Single 9409 in corn,
Control group applies N, P per acre2O5、K2O is respectively 20,10,16kg；
Test group applies complex microorganism function bacterial manure (N, P of the present invention per acre2O5、K2O >=5%, function bacterium sum >=0.2
Hundred million/gram) 150kg.
2/3 conduct base fertilizer of whole fertilizer applies, and 1/3 conduct top dressing of remaininging is spreaded manuer in holes in the typhon mouth phase.
Field management, watering etc. routinely carry out.
Influence of the 3 microbial function bacterial manure of table to corn yield and quality
Test group reduces 67.4%N, P while application microbial function bacterial manure2O5、K2The dosage of O is reducing nothing
The yield and quality of corn is increased while machine fertilizer.Can clearly it see from the test result of upper table 3, test group yield
11.75% is increased, while the quality of corn is also greatly increased, especially lysine content increases 8.20%,
The content of protein and fat also has different degrees of raising, this has the high-lysine corn for being mainly used for feed important product
Matter raising acts on.
Embodiment 10
Cotton is tested using complex microorganism function bacterial manure application effect
Crop varieties：Cotton, kind are " Handan cotton 886 ".
Conventional fertilizer application：Base fertilizer Common compound fertilizer (N, P2O5、K2Each 15%) 60 kgs/acre of O, 15 kilograms of urea of flower bud phase top dressing.
Experiment fertilizer：Base fertilizer applies complex microorganism function bacterial manure (N, P of the present invention2O5、K2O >=5%, function bacterium sum >=
0.2 hundred million/gram) 150 kgs/acre, 15 kilograms of urea of flower bud phase top dressing.
Experimental design：It repeats three times.
Cotton was sowed May 1, flower bud phase top dressing, weeding, watering Routine Management.
Test result：
Growth period is observed：Conventional fertilizer application growth is normal；Neatly healthy and strong using the then emergence of microbial function bacterial manure, plant is steady
Strong, leaf dark green, Bolls per plant and Single boll weight have increase.
Yield comparison：Per mu yield using microbial function bacterial manure is 609 kilograms, is increased than conventional fertilizer application (513 kilograms of per mu yield)
96 kilograms of production, rate of growth 18.7%.
Embodiment 11
Cotton applies the application effect of complex microorganism function bacterial manure
Experimental cultivar：Weighing apparatus cotton No. four.
Test site：Tangshan City Fengnan District pony village town, experimental field continuous plant cotton 10 years or more, droop, red autumnal leaves
Stem wilt development is serious, and physiological disease is serious caused by continuous cropping continuous cropping and soil potassium deficiency.
For trying fertilizer：It demonstrates (complex microorganism function bacterial manure of the present invention), control (conventional composite fertilizer+Gen Meifu microorganisms
Bacterial manure).
Experiment sets composite microbic bacterial fertilizer and 4 kgs/acre of conventional fertilizer application+bottom application processing of Gen Meifu two, repeats three times, with
Machine arranges, per 20 × 6M of plot area2.It includes protection row 900M that experiment, which is taken up an area,2.April 18 site preparation and fertilization.It ploughs, sow and pours
The agronomic measures such as water, fertilising, intertillage are identical.
After planting 120 days investigation disease indexs and preventive effect, computational methods are as follows：
4 cotton disease index grade scale of table
As a result with analysis
Growth Character and yield investigation are shown in Table 5 and table 6
5 Growth Character of table is investigated
6 cell production original table of table
| Reprocessing | R1 | R2 | R3 | It is average | Rate of growth (%) |
| CK (control) | 14.6 | 15.3 | 15.2 | 15.04 | |
| T (demonstration) | 16.5 | 16.6 | 17.26 | 16.79 | 11.6% |
T test Analysis is carried out to cell production：SD=0.3235, t=5.409；Through looking into t0.01=4.604, t0.05=
2.776, t values are significantly greater than t0.01, t0.05 respectively, it is seen that there are notable differences between processing.That is complex microorganism function bacterial manure
Product can significantly improve output of cotton.The droop of early period and the red leaf blight in later stage can be effectively prevented simultaneously.And it applies
There is not early ageing sign in the cotton of complex microorganism function bacterial manure.
Protection effect is shown in Table 7.
7 different disposal of table counts various soil-borne disease control effects
(disease index is investigated：It is withered and because the red autumnal leaves degree of drying up determines single plant incidence with plant, from disease-free to disease
Seriously, it is determined as 0,1,2,3,4 five rank)
Bottom application complex microorganism function bacterial fertilizer products of the present invention can obviously increase output of cotton, and increase rate is up to 11.6%；
There is certain facilitation to cotton nutrient growth and reproductive growth, it is especially apparent to yield composition factor facilitation, it is single
Strain Cheng Ling relatively controls increase 0.9, and bell increases 0.2 gram again, and lint percentage improves 0.53 percentage point.The complex microorganism of the present invention simultaneously
Function bacterial manure can also effectively mitigate the generation of droop early period and later stage red leaf blight, and preventive effect is respectively up to 51.4% He
61%.
Embodiment 12
The application effect of rice application complex biological function bacterial manure
Test site：Three farm Japanese plum justice of Tanghai County, Hebei Province, 35 mu of area.
Fertilizing method：Model field applies 100 kilograms of complex microorganism function bacterial manure of the present invention and routine respectively with field is compareed
Bio-bacterial manure (containing bacillus subtilis 0.2 hundred million/gram).Period of seedling establishment imposes 10 kgs/acre of urea, other transplanting dates, water respectively
It is essentially identical to be in charge of reason.
Field investigation：Time of infertility performance is as follows：The rice seedling of model field robust growth always, leaf color is glossy dark green, fruit ear
The apparent fruit ear than compareing field is long and big；Not only yield is high, and falls yellow good, full seed.
It is as follows that rice maturity investigates and analyses table：
8 different disposal rice maturity of table investigates and analyses situation
Interpretation of result：
It is relatively applied using 20 mu of model fields of the complex microorganism function bacterial manure of the present invention by maturity period field investigation
The control field of standard biologic bacterial manure, plant height increase by 1.7 centimetres, and spike length increases by 0.9 centimetre, and every square metre of mature spike number increases by 22,
Grain number per spike increases by 1.9, and mass of 1000 kernel increases by 0.2 gram, and mu increases production 60.2 kilograms, amplification 10.5%.
The above is only a preferred embodiment of the present invention, it is noted that for the ordinary skill people of the art
For member, various improvements and modifications may be made without departing from the principle of the present invention, these improvements and modifications are also answered
It is considered as protection scope of the present invention.
Claims (10)
1. a kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure, are obtained by complex micro organism fungicide aerobic fermentation fowl and animal excrement,
The complex micro organism fungicide includes mass ratio 3~5:2~3:1~2 nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3.
2. complex microorganism function bacterial manure according to claim 1, which is characterized in that in the complex micro organism fungicide,
Thalline total quantity be more than 4,700,000,000/gram.
3. complex microorganism function bacterial manure according to claim 1 or 2, which is characterized in that the complex micro organism fungicide
In, the living bacteria count of nitrogen-fixing bacteria N1 is more than 2,000,000,000/gram, the living bacteria count of phosphate solubilizing bacteria FL7 is more than 200,000,000/gram, potassium solubilizing bacteria K3's
Living bacteria count be more than 2,500,000,000/gram.
4. complex microorganism function bacterial manure according to claim 1, which is characterized in that the complex microorganism function bacterial manure
Thalline total quantity be more than 0.2 hundred million/gram.
5. complex microorganism function bacterial manure according to claim 1, which is characterized in that the complex micro organism fungicide and institute
The weight ratio for stating fowl and animal excrement is 1:20-25 ten thousand.
6. according to the preparation method of complex microorganism function bacterial manure described in Claims 1 to 5 any one, include the following steps：
(1) nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 and potassium solubilizing bacteria K3 are fermented in respective fermentation medium respectively, when in zymotic fluid
When the spore forming rate Da Dao≤80% of nitrogen-fixing bacteria N1, phosphate solubilizing bacteria FL7 or potassium solubilizing bacteria K3, terminate fermentation；According to nitrogen-fixing bacteria N1, solution
The mass ratio 3-5 of phosphorus bacterium FL7 and potassium solubilizing bacteria K3:2-3:1-2 batch mixings, obtain complex micro organism fungicide；
(2) by the complex micro organism fungicide and fowl and animal excrement according to weight ratio 1:20-25 ten thousand is mixed, good at 40 DEG C -70 DEG C
Oxygen compost fermentation 12-15 days obtains just fermented manure；
(3) the just fermented manure is subjected to 40-45 days secondary naturally decomposed, obtains complex microorganism function bacterial manure.
7. the preparation method of complex microorganism function bacterial manure according to claim 6, which is characterized in that the nitrogen-fixing bacteria N1's
Fermentation medium by weight percentage, including mannitol 0.5-1.5%, potassium dihydrogen phosphate 0.005-0.03%, magnesium sulfate
0.005-0.03%, sodium chloride 0.005-0.03%, calcium sulfate 0.005-0.02%, calcium carbonate 0.3-0.7%, agar 1.0-
3.0%, remaining is water；The pH value of the nitrogen-fixing bacteria N1 fermentation mediums is 6.0-7.0.
8. the preparation method of complex microorganism function bacterial manure according to claim 6, which is characterized in that the phosphate solubilizing bacteria FL7
Fermentation medium by weight percentage, including sulfuric acid diamino 0.03-0.06%, potassium chloride 0.001-0.004%, sodium chloride
0.001-0.004%, green-vitriol 0.0001-0.0004%, manganese sulfate monohydrate 0.0001-0.0004%, epsom salt
0.001-0.004%, glucose 0.5-1.5%, yeast powder 0.03-0.07%, calcium phosphate 0.3-0.6%, agar 1.0-
3.0%, remaining is water；The pH value of the phosphate solubilizing bacteria FL7 fermentation mediums is 7-7.5.
9. the preparation method of complex microorganism function bacterial manure according to claim 6, which is characterized in that the potassium solubilizing bacteria K3's
Fermentation medium by weight percentage, including disodium hydrogen phosphate 0.15-0.25%, trichlorine iron 0.0004-0.0006%, seven water
Magnesium sulfate 0.04-0.06%, calcium carbonate 0.005-0.015%, sucrose 0.2-0.4%, feldspar in powder 0.05-0.15%, remaining
For water；The pH value of the potassium solubilizing bacteria K3 fermentation mediums is 7.0-7.5.
10. according to the preparation method of complex microorganism function bacterial manure described in claim 6~9 any one, which is characterized in that institute
State it is secondary it is naturally decomposed after further include：
By the secondary naturally decomposed material screening；The obtained rough material of sieving is mixed with fowl and animal excrement, again by multiple
Microbial bacterial agent fermentation is closed, complex microorganism function bacterial manure is made.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810541033.5A CN108484284A (en) | 2018-05-30 | 2018-05-30 | A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810541033.5A CN108484284A (en) | 2018-05-30 | 2018-05-30 | A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN108484284A true CN108484284A (en) | 2018-09-04 |
Family
ID=63351509
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810541033.5A Pending CN108484284A (en) | 2018-05-30 | 2018-05-30 | A kind of phosphorus decomposing, potassium fixed nitrogen complex microorganism function bacterial manure and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108484284A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112279697A (en) * | 2020-11-12 | 2021-01-29 | 中国农业科学院果树研究所 | Biological bacterial fertilizer and preparation method and application thereof |
| CN115448805A (en) * | 2022-10-20 | 2022-12-09 | 河北福满硒生物科技有限公司 | Biological organic fertilizer |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103664259A (en) * | 2013-12-09 | 2014-03-26 | 河南中宏康馨农业有限公司 | Method for fermentation production of bio-organic fertilizer by using duck manure |
| CN105254356A (en) * | 2015-09-23 | 2016-01-20 | 西北农林科技大学 | Bioorganic fertilizer used for planting organic vegetables, and preparation method thereof |
| CN105777234A (en) * | 2016-05-31 | 2016-07-20 | 沈阳市现代三川生物技术研究所 | Method for preparing biological organic fertilizer through common fermentation of nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium bacteria |
| CN107098746A (en) * | 2017-04-27 | 2017-08-29 | 中国农业科学院麻类研究所 | A kind of capsicum Special compound microbial fertilizer and preparation method thereof |
-
2018
- 2018-05-30 CN CN201810541033.5A patent/CN108484284A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103664259A (en) * | 2013-12-09 | 2014-03-26 | 河南中宏康馨农业有限公司 | Method for fermentation production of bio-organic fertilizer by using duck manure |
| CN105254356A (en) * | 2015-09-23 | 2016-01-20 | 西北农林科技大学 | Bioorganic fertilizer used for planting organic vegetables, and preparation method thereof |
| CN105777234A (en) * | 2016-05-31 | 2016-07-20 | 沈阳市现代三川生物技术研究所 | Method for preparing biological organic fertilizer through common fermentation of nitrogen-fixing bacteria, phosphate solubilizing bacteria and potassium bacteria |
| CN107098746A (en) * | 2017-04-27 | 2017-08-29 | 中国农业科学院麻类研究所 | A kind of capsicum Special compound microbial fertilizer and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 胡宗福: "菌糠菌肥在修复盐碱土和锌铜镍铬污染土壤中的应用研究", 《中国优秀硕士学位论文全文数据库（电子期刊） 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN112279697A (en) * | 2020-11-12 | 2021-01-29 | 中国农业科学院果树研究所 | Biological bacterial fertilizer and preparation method and application thereof |
| CN115448805A (en) * | 2022-10-20 | 2022-12-09 | 河北福满硒生物科技有限公司 | Biological organic fertilizer |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN100579940C (en) | Method for preparing biologic organic fertilizer for preventing and curing eelworm harm and use thereof | |
| CN104560828B (en) | Microbial agent and application in prospect of passivating heavy metals of soil | |
| CN102487621B (en) | Method for efficiently reducing continuous cropping obstacles of facility soil | |
| CN101724560B (en) | Microbial inoculums and preparation method thereof | |
| CN104429581B (en) | A kind of Organic tomato health cultural method | |
| CN102199049B (en) | Microbial organic manure and preparation method thereof | |
| CN104609997A (en) | Manufacturing process and application method of ginseng bio-organic fertilizer | |
| CN104396486B (en) | A kind of implantation methods of winter wheat | |
| CN106386163A (en) | Method for cultivating edible fungi by using crop straw | |
| CN103848698A (en) | Biological organic fertilizer prepared by aerobic fermentation of biogas residue and preparation method thereof | |
| CN101130465A (en) | Grain type humic acid compound biological fertilizer and manufacturing method therefor | |
| CN103168668A (en) | Fruit and vegetable seedling culturing substrate prepared by utilizing agricultural wastes | |
| CN107840762A (en) | A kind of method that biological organic fertilizer is made using cow dung | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN1398824A (en) | Bioorganic fertilizer and its production process | |
| CN105418335A (en) | Beneficial biological bacterium fermented microbial organic fertilizer | |
| CN100460501C (en) | Organism preparation method of agricultural amino acid and fertilizer product | |
| CN105777229A (en) | Organic fermented fertilizer for raising selenium content of vegetables, production method and cultivation method | |
| CN109852565A (en) | A kind of salt-soda soil composite modifying agent and its method of administration | |
| CN107840720A (en) | A kind of method that biological organic fertilizer is made using pig manure | |
| CN109479671A (en) | A kind of domesticating and cultivating matrix and preparation method thereof improving tuber of pinellia tissue culture shoot survival percent | |
| CN107580818A (en) | A kind of soil conditioning and the integrated approach repaired | |
| CN105565959A (en) | Soil ecological system restoring organic fertilizer | |
| CN107382496A (en) | A kind of artificial soil for strengthening crop anti-adversity and the method for strengthening crop anti-adversity | |
| CN107324926A (en) | Suitable for the improving fertilizer and its production method in salt-soda soil |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |