CN113233933A - Microbial agent for promoting early growth and fast growth of tobacco plants and preparation method and application thereof - Google Patents
Microbial agent for promoting early growth and fast growth of tobacco plants and preparation method and application thereof Download PDFInfo
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- 230000012010 growth Effects 0.000 title claims abstract description 105
- 235000002637 Nicotiana tabacum Nutrition 0.000 title claims abstract description 100
- 230000000813 microbial effect Effects 0.000 title claims abstract description 95
- 244000061176 Nicotiana tabacum Species 0.000 title claims abstract description 56
- 230000001737 promoting effect Effects 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims abstract description 10
- 239000000843 powder Substances 0.000 claims abstract description 57
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 52
- 241000208125 Nicotiana Species 0.000 claims abstract description 44
- 150000001875 compounds Chemical class 0.000 claims abstract description 22
- PUKLDDOGISCFCP-JSQCKWNTSA-N 21-Deoxycortisone Chemical compound C1CC2=CC(=O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@@](C(=O)C)(O)[C@@]1(C)CC2=O PUKLDDOGISCFCP-JSQCKWNTSA-N 0.000 claims abstract description 20
- 229920002101 Chitin Polymers 0.000 claims abstract description 20
- FCYKAQOGGFGCMD-UHFFFAOYSA-N Fulvic acid Natural products O1C2=CC(O)=C(O)C(C(O)=O)=C2C(=O)C2=C1CC(C)(O)OC2 FCYKAQOGGFGCMD-UHFFFAOYSA-N 0.000 claims abstract description 20
- 108010020346 Polyglutamic Acid Proteins 0.000 claims abstract description 20
- 239000002509 fulvic acid Substances 0.000 claims abstract description 20
- 229940095100 fulvic acid Drugs 0.000 claims abstract description 20
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 20
- 239000011707 mineral Substances 0.000 claims abstract description 20
- NCYVXEGFNDZQCU-UHFFFAOYSA-N nikethamide Chemical compound CCN(CC)C(=O)C1=CC=CN=C1 NCYVXEGFNDZQCU-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229920002643 polyglutamic acid Polymers 0.000 claims abstract description 20
- 238000013329 compounding Methods 0.000 claims abstract description 18
- 239000003895 organic fertilizer Substances 0.000 claims abstract description 16
- 241000894006 Bacteria Species 0.000 claims abstract description 11
- 244000005700 microbiome Species 0.000 claims abstract description 8
- 238000004321 preservation Methods 0.000 claims description 30
- 239000002131 composite material Substances 0.000 claims description 29
- 230000001580 bacterial effect Effects 0.000 claims description 24
- 241000193744 Bacillus amyloliquefaciens Species 0.000 claims description 12
- 244000063299 Bacillus subtilis Species 0.000 claims description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 12
- 241000193830 Bacillus <bacterium> Species 0.000 claims description 10
- 235000015097 nutrients Nutrition 0.000 claims description 8
- 240000002791 Brassica napus Species 0.000 claims description 6
- 235000004977 Brassica sinapistrum Nutrition 0.000 claims description 6
- 238000000034 method Methods 0.000 claims description 5
- 239000000203 mixture Substances 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims 1
- 238000009335 monocropping Methods 0.000 abstract description 9
- 239000003337 fertilizer Substances 0.000 abstract description 7
- 230000008635 plant growth Effects 0.000 abstract 1
- 230000002786 root growth Effects 0.000 abstract 1
- 238000011282 treatment Methods 0.000 description 41
- 230000009418 agronomic effect Effects 0.000 description 12
- 239000002689 soil Substances 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 239000002068 microbial inoculum Substances 0.000 description 5
- 238000009629 microbiological culture Methods 0.000 description 4
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 description 3
- 238000011161 development Methods 0.000 description 3
- 230000018109 developmental process Effects 0.000 description 3
- 230000004720 fertilization Effects 0.000 description 3
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 description 3
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 2
- 238000009826 distribution Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000035558 fertility Effects 0.000 description 2
- 238000003306 harvesting Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 230000002906 microbiologic effect Effects 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000005416 organic matter Substances 0.000 description 2
- 230000000644 propagated effect Effects 0.000 description 2
- 239000000779 smoke Substances 0.000 description 2
- 238000006467 substitution reaction Methods 0.000 description 2
- 229930192334 Auxin Natural products 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 230000003698 anagen phase Effects 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005200 bud stage Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 238000013400 design of experiment Methods 0.000 description 1
- 238000001784 detoxification Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 238000003973 irrigation Methods 0.000 description 1
- 230000002262 irrigation Effects 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000000691 measurement method Methods 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 239000004323 potassium nitrate Substances 0.000 description 1
- 235000010333 potassium nitrate Nutrition 0.000 description 1
- OTYBMLCTZGSZBG-UHFFFAOYSA-L potassium sulfate Chemical compound [K+].[K+].[O-]S([O-])(=O)=O OTYBMLCTZGSZBG-UHFFFAOYSA-L 0.000 description 1
- 229910052939 potassium sulfate Inorganic materials 0.000 description 1
- 235000011151 potassium sulphates Nutrition 0.000 description 1
- 239000000021 stimulant Substances 0.000 description 1
- 238000012876 topography Methods 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 238000010200 validation analysis Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05F—ORGANIC FERTILISERS NOT COVERED BY SUBCLASSES C05B, C05C, e.g. FERTILISERS FROM WASTE OR REFUSE
- C05F5/00—Fertilisers from distillery wastes, molasses, vinasses, sugar plant or similar wastes or residues, e.g. from waste originating from industrial processing of raw material of agricultural origin or derived products thereof
- C05F5/006—Waste from chemical processing of material, e.g. diestillation, roasting, cooking
- C05F5/008—Waste from biochemical processing of material, e.g. fermentation, breweries
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G22/00—Cultivation of specific crops or plants not otherwise provided for
- A01G22/45—Tobacco
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/80—Soil conditioners
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- Life Sciences & Earth Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Botany (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Forests & Forestry (AREA)
- Ecology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Pest Control & Pesticides (AREA)
- Environmental & Geological Engineering (AREA)
- Toxicology (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Wood Science & Technology (AREA)
- Soil Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention discloses a microbial agent for promoting early growth and fast growth of tobacco plants, a preparation method and application thereof, relates to the technical field of fertilizers, and solves the technical problem of continuous cropping of tobacco all the year round in the prior art. The microbial agent for promoting the early growth and the quick growth of tobacco plants comprises the following components in percentage by mass: 1 to 2 percent of compound microorganism bacterium powder, 1.8 to 4.6 percent of biostimulant and 93.4 to 97.2 percent of carrier, wherein the sum of the mass percentages of the components is 100 percent; the compound microbial powder is prepared by compounding a strain WSWFJ39, a strain WSWFJ41 and a strain WSWFJ 46; the biological stimulin is one or more of mineral fulvic acid, chitin and polyglutamic acid; the carrier is an organic fertilizer. The microbial agent for promoting the early growth and the quick growth of the tobacco plants is used in the field of planting of tobacco, and can promote the early growth and the quick growth of the tobacco plants and coordinate the consistency of root growth and tobacco plant growth; meanwhile, the stress resistance of tobacco plants can be enhanced, the economic character of flue-cured tobacco is improved, and the quality of tobacco leaves is improved.
Description
Technical Field
The invention relates to the technical field of fertilizers, in particular to a microbial agent for promoting early growth and quick growth of tobacco plants, and a preparation method and application thereof.
Background
Tobacco is an important economic crop in Yunnan, however, continuous cropping obstacles are increasingly prominent in part of tobacco regions due to continuous cropping of the tobacco for a long time, so that the root system of a tobacco plant grows badly, the early growth of the tobacco plant is slow, the stress resistance is poor, and finally, the nutrition of the tobacco plant is unbalanced, the yield of tobacco leaves is reduced, and the quality of the tobacco leaves is reduced.
In order to overcome the continuous cropping obstacle of flue-cured tobacco and promote the early growth and quick growth of tobacco plants, in recent years, the application of mineral-derived biological stimulants such as fulvic acid, chitin, polyglutamic acid and the like and a compound microbial agent for resisting diseases and promoting growth is common, but the effect is not obvious or unstable. Therefore, providing a fertilizer capable of overcoming the continuous cropping obstacle of flue-cured tobacco becomes a technical problem to be solved by the technical personnel in the field.
Disclosure of Invention
One of the purposes of the invention is to provide a microbial agent for promoting the early growth and fast growth of tobacco strains, a preparation method and application thereof, and to solve the technical problems of the prior art caused by continuous cropping of tobacco all the year round. The technical effects that can be produced by the preferred technical scheme of the invention are explained in detail in the following.
In order to achieve the purpose, the invention provides the following technical scheme:
the invention relates to a microbial agent for promoting the early growth and quick growth of tobacco plants, which comprises the following components in percentage by mass: 1 to 2 percent of compound microorganism bacterium powder, 1.8 to 4.6 percent of biostimulant and 93.4 to 97.2 percent of carrier, wherein the sum of the mass percentages of the components is 100 percent; the compound microbial powder is prepared by compounding a strain WSWFJ39, a strain WSWFJ41 and a strain WSWFJ 46; the strain WSWFJ39 is bacillus amyloliquefaciens with the preservation number of CGMCC No.20147, the strain WSWFJ41 is bacillus with the preservation number of CGMCC No.20151, and the strain WSWFJ46 is bacillus subtilis with the preservation number of CGMCC No. 20145; the biological stimulin is one or more of mineral fulvic acid, chitin and polyglutamic acid; the carrier is an organic fertilizer.
According to a preferred embodiment, the effective viable count of the composite microbial powder is more than 1.0X 109cfu/g。
According to a preferred embodiment, the effective viable bacteria quantity ratio of the strain WSWJ39, the strain WSWJ41 and the strain WSWJ46 in the composite microbial powder is 2: 1.
According to a preferred embodiment, the bio-stimulant comprises the following components in percentage by mass: 1 to 3 percent of mineral fulvic acid, 0.3 to 0.6 percent of chitin and 0.5 to 1 percent of polyglutamic acid. The mass percentages of the mineral fulvic acid, the chitin and the polyglutamic acid are relative to the microbial agent for promoting the early growth and the quick growth of the tobacco plant.
According to a preferred embodiment, the carrier is an organic fertilizer obtained by fermenting pure rapeseed cakes, and the carrier has an organic matter content of not less than 80% and a total nutrient content of not less than 8.0%.
The preparation method of the microbial agent for promoting the early growth and the quick growth of tobacco plants comprises the following steps:
s1: compounding compound microbial bacteria powder, namely, respectively detecting the viable count of the bacterial powder of the bacterial strain WSWFJ39, the bacterial strain WSWFJ41 and the bacterial powder of the bacterial strain WSWFJ46, and then compounding the bacterial powder according to the ratio of the effective viable count to the effective viable count of 2: 1;
s2: mixing biological stimulin with composite microbial powder, and mixing the composite microbial powder obtained by compounding the S1 with one or more of mineral fulvic acid, chitin and polyglutamic acid uniformly;
s3: preparing a microbial agent for promoting the early growth and the quick growth of tobacco plants, namely adding a mixture of the biostimulant obtained in the step S2 and the composite microbial powder into a carrier organic fertilizer, fully and uniformly mixing, and extruding and granulating to obtain the microbial agent for promoting the early growth and the quick growth of the tobacco plants;
wherein the composite microbial powder, the biological stimulin and the carrier respectively comprise the following components in percentage by mass: 1-2% of composite microbial powder, 1.8-4.6% of biological stimulin and 93.4-97.2% of carrier, wherein the sum of the mass percentages of the components is 100%;
wherein the strain WSWFJ39 in the step S1 is Bacillus amyloliquefaciens with the preservation number of CGMCC No.20147, the strain WSWFJ41 is Bacillus with the preservation number of CGMCC No.20151, and the strain WSWFJ46 is Bacillus subtilis with the preservation number of CGMCC No. 20145.
According to a preferred embodiment, in step S1, the effective viable count of the composite microbial powder is greater than 1.0 × 109cfu/g。
According to a preferred embodiment, in step S2, the biostimulant includes mineral fulvic acid, chitin and polyglutamic acid, and the mass percentages of the components are as follows: 1 to 3 percent of mineral source fulvic acid, 0.3 to 0.6 percent of chitin and 0.5 to 1 percent of polyglutamic acid. The mass percentages of the mineral fulvic acid, the chitin and the polyglutamic acid are relative to the microbial agent for promoting the early growth and the quick growth of the tobacco plant.
According to a preferred embodiment, in step S3, the carrier organic fertilizer is an organic fertilizer obtained by fermenting pure rapeseed cake, and the carrier has an organic matter content of not less than 80% and a total nutrient content of not less than 8.0%.
The microbial agent for promoting the early growth and the quick growth of tobacco plants, which is disclosed by any technical scheme of the invention, is applied to tobacco planting.
The microbial agent for promoting the early growth and the quick growth of tobacco plants, the preparation method and the application thereof provided by the invention at least have the following beneficial technical effects:
the microbial agent for promoting the early growth and the quick growth of the tobacco plants is prepared by mixing compound microbial powder, biological stimulin and a carrier, and after the microbial agent is applied to soil, the compound microorganisms can be rapidly propagated, so that toxic substances in the continuous cropping soil of the tobacco plants can be decomposed, the effectiveness of nutrients such as phosphorus and potassium in the soil can be improved, auxin such as IAA (amino acid acetic acid) is secreted, and the growth of the root systems of the tobacco plants can be promoted; the biological stimulin can improve the stress resistance of tobacco plants, promote the absorption of roots to nutrients and stimulate the growth and development of the tobacco plants; the organic fertilizer as a carrier can keep the activity of the compound microbial inoculum and the biological stimulin on one hand, and can provide initial nutrition for the compound microbial inoculum on the other hand, so that the microorganisms can be rapidly propagated after being applied to soil.
The microbial agent for promoting the early growth and the quick growth of tobacco plants is prepared by compounding a compound microbial agent and biological stimulin aiming at the characteristics of tobacco, integrates the detoxification, the live soil and the growth promotion of microorganisms and the stress resistance, the growth promotion, the yield increase and the quality improvement of the biological stimulin into a whole, and can be used in the field of planting of tobacco, so that the microbial agent not only can promote the early growth and the quick growth of the tobacco plants and coordinate the consistency of the growth of root systems and the growth of the tobacco plants; meanwhile, the stress resistance of tobacco plants can be enhanced, the economic character of flue-cured tobacco is improved, the quality of tobacco leaves is improved, and the technical problem of continuous cropping of tobacco all the year round in the prior art is solved.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be described in detail below. It is to be understood that the described embodiments are merely exemplary of the invention, and not restrictive of the full scope of the invention. All other embodiments, which can be derived by a person skilled in the art from the examples given herein without making any creative effort, shall fall within the protection scope of the present invention.
The application of the microbial agent for promoting the early growth and fast growth of tobacco strains in tobacco planting according to the invention is described in detail in the following with reference to examples 1 and 2.
Example 1
The microbial agent for promoting the early growth and the quick growth of the tobacco plants in the embodiment comprises 1% of composite microbial powder, 1.8% of biostimulant and 97.2% of carrier in mass ratio.
The composite microbial powder of the embodiment is prepared by compounding a bacillus amyloliquefaciens strain, a bacillus strain and a bacillus subtilis strain, wherein:
bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the number of WSWFJ39 and the preservation registration number of CGMCC No.20147 and the preservation date: 28/06/2020, depository: general microbiological center of China Committee for culture Collection of microorganisms, the preservation address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North;
bacillus (Bacillus sp.), the number of which is WSWFJ41, the preservation registration number is CGMCC number 20151, and the preservation date is as follows: 28/06/2020, depository: china general microbiological culture Collection center, preservation Address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North;
bacillus subtilis (Bacillus subtilis) with the number of WSWFJ46 and the preservation registration number of CGMCC No.20145 and the preservation date: 28/06/2020, depository: china general microbiological culture Collection center, preservation Address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The effective viable count of the composite microbial powder is more than 1.0 multiplied by 109cfu/g。
The effective viable bacteria quantity ratio of the composite microbial powder, namely the bacillus amyloliquefaciens, the bacillus and the bacillus subtilis is 2: 1.
The biological stimulator comprises the following components in percentage by mass: 1% of mineral source fulvic acid, 0.3% of chitin and 0.5% of polyglutamic acid.
The carrier is an organic fertilizer fermented by pure rapeseed dregs, the content of organic matters is not less than 80 percent, and the content of total nutrients is not less than 8.0 percent.
The preparation method of the microbial agent for promoting the early growth and the quick growth of the tobacco plants comprises the following steps:
s1: compounding compound microbial bacteria powder, namely, respectively detecting the viable count of the bacterial powder of the bacterial strain WSWFJ39, the bacterial strain WSWFJ41 and the bacterial powder of the bacterial strain WSWFJ46, and then compounding the bacterial powder according to the ratio of the effective viable count to the effective viable count of 2: 1;
s2: mixing biological stimulin with composite microbial powder, and uniformly mixing the composite microbial powder obtained by compounding the S1 with mineral fulvic acid, chitin and polyglutamic acid;
s3: preparing a microbial agent for promoting the early growth and the quick growth of tobacco plants, namely adding a mixture of the biostimulant obtained in the step S2 and the composite microbial powder into a carrier organic fertilizer, fully and uniformly mixing, and extruding and granulating to obtain the microbial agent for promoting the early growth and the quick growth of the tobacco plants;
wherein the composite microbial powder, the biological stimulin and the carrier respectively comprise the following components in percentage by mass: 1% of compound microbial powder, 1.8% of biological stimulin and 97.2% of carrier;
wherein the biological stimulator comprises the following components in percentage by mass: 1% of mineral source fulvic acid, 0.3% of chitin and 0.5% of polyglutamic acid.
Example 2
The microbial agent for promoting the early growth and the quick growth of the tobacco strains in the embodiment is composed of 2% of composite microbial powder, 4.6% of biostimulant and 93.4% of carrier in mass ratio.
The composite microbial powder of the embodiment is prepared by compounding a bacillus amyloliquefaciens strain, a bacillus strain and a bacillus subtilis strain, wherein:
bacillus amyloliquefaciens (Bacillus amyloliquefaciens) with the number of WSWFJ39 and the preservation registration number of CGMCC No.20147 and the preservation date: 28/06/2020, depository: general microbiological center of China Committee for culture Collection of microorganisms, the preservation address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North;
bacillus (Bacillus sp.), the number of which is WSWFJ41, the preservation registration number is CGMCC number 20151, and the preservation date is as follows: 28/06/2020, depository: china general microbiological culture Collection center, preservation Address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North;
bacillus subtilis (Bacillus subtilis) with the number of WSWFJ46 and the preservation registration number of CGMCC No.20145 and the preservation date: 28/06/2020, depository: china general microbiological culture Collection center, preservation Address: xilu No. 1 Hospital No. 3, Beijing, Chaoyang, North.
The effective viable count of the composite microbial powder is more than 1.0 multiplied by 109cfu/g。
The effective viable bacteria quantity ratio of the composite microbial powder, namely the bacillus amyloliquefaciens, the bacillus and the bacillus subtilis is 2: 1.
The biological stimulator comprises the following components in percentage by mass: 3% of mineral source fulvic acid, 0.6% of chitin and 1% of polyglutamic acid.
The carrier is an organic fertilizer fermented by pure rapeseed dregs, the content of organic matters is not less than 80 percent, and the content of total nutrients is not less than 8.0 percent.
The preparation method of the microbial agent for promoting the early growth and the quick growth of the tobacco plants comprises the following steps:
s1: compounding compound microbial bacteria powder, namely, respectively detecting the viable count of the bacterial powder of the bacterial strain WSWFJ39, the bacterial strain WSWFJ41 and the bacterial powder of the bacterial strain WSWFJ46, and then compounding the bacterial powder according to the ratio of the effective viable count to the effective viable count of 2: 1;
s2: mixing biological stimulin with composite microbial powder, and uniformly mixing the composite microbial powder obtained by compounding the S1 with mineral fulvic acid, chitin and polyglutamic acid;
s3: preparing a microbial agent for promoting the early growth and the quick growth of tobacco plants, namely adding a mixture of the biostimulant obtained in the step S2 and the composite microbial powder into a carrier organic fertilizer, fully and uniformly mixing, and extruding and granulating to obtain the microbial agent for promoting the early growth and the quick growth of the tobacco plants;
wherein the composite microbial powder, the biological stimulin and the carrier respectively comprise the following components in percentage by mass: 2% of compound microbial powder, 4.6% of biological stimulin and 93.4% of carrier;
wherein the biological stimulator comprises the following components in percentage by mass: 3% of mineral source fulvic acid, 0.6% of chitin and 1% of polyglutamic acid.
The products of example 1 and example 2 were used for validation: the influence of the microbial agent for promoting the early growth and the rapid growth of the tobacco plants on the growth and the development of the tobacco plants is explored, and the effect of the microbial agent for promoting the early growth and the rapid growth of the tobacco plants in the aspect of promoting the early growth and the rapid growth of the tobacco plants is verified.
Materials and methods
1. Time and place of experiment
The test was conducted in Shangtong city, Shandong county, Wenzhai-Ann Cuckura, between 4 and 9 months in 2020. The continuous cropping of the tobacco in the test field can be up to 12 years, the test field has flat topography, moderate soil fertility, uniform fertility distribution and good illumination and irrigation and drainage conditions.
2. Test material
The test varieties are: yunyan 87;
fertilizer to be tested: compound fertilizer (N-P) special for tobacco2O5-K2O11-14-22, potassium nitrate (N-P)2O5-K213.5-0-43.5% of O, potassium sulfate (K)2O.gtoreq.50%), the functional microbial agent of example 1 and the functional microbial agent of example 2.
3. Design of experiments
The experiment was set to 3 treatments:
and (3) treatment A: local conventional fertilization;
and (B) treatment: local conventional fertilization plus 2 kg/mu of the functional microbial inoculum of example 1;
and C, treatment: local conventional fertilization plus 2 kg/mu of the functional microbial inoculum of example 2.
The amounts of the fertilizers used for each treatment are shown in Table 1.
TABLE 1 Fertilizer consumption table for each treatment
4. Survey item and method
4.1 major childbearing period notes
The time of the transplanting period, the root-growing period, the vigorous growing period, the bud period, the capping period, the mature period of the foot leaves and the mature period of the top leaves and the full growth period of each treatment are recorded.
4.2 agronomic trait survey
After the vigorous growth period and the capping period are respectively carried out, 15 representative tobacco plants are randomly selected according to the S-shaped distribution in each treatment, and are investigated according to the YC/T142-2010 tobacco agronomic character investigation and measurement method, and the plant height, the stem circumference, the leaf number, the leaf length, the leaf width and the like are observed and measured.
4.3 root weight survey statistics
After the collection and the baking are finished, 3 representative tobacco plants are randomly selected in each treatment, and the fresh weight and the dry weight of the root system of each tobacco plant are weighed and recorded.
4.4 economic traits statistics
The treatments are respectively roasted when the fruits are normally mature, and classified and sold according to the same standard. And (4) counting economic traits of the flue-cured tobacco to be treated, including acre yield, acre yield value, average price, first-class tobacco proportion and middle-class tobacco proportion.
Second, results and analysis
1. Development of the fertile phase
As can be seen from Table 2, the time for the treatment B and the treatment C to enter the mass stage is respectively 3 days and 4 days earlier and the time for the treatment C to enter the vigorous growth stage is respectively 5 days and 7 days earlier than the control of the treatment A, which shows that the application of the functional microbial agent of the embodiment 1 and the embodiment 2 can promote the early growth and the fast growth of the tobacco plants and lead the tobacco plants to enter the mass stage and the vigorous growth stage in advance. The time of each treatment entering the bud stage, the capping stage and the tobacco leaf mature stage is basically consistent; compared with the control of the treatment A, the time for finishing the baking of the treatment B and the treatment C is slightly delayed, which indicates that the application of the functional microbial agent can improve the nutrient tolerance of the tobacco leaves to a certain extent.
TABLE 2 growth phase progression
2. Agronomic traits
The results of the agronomic trait survey for the vigorous treatment periods are shown in Table 3. As can be seen from the table, the agronomic performance indexes such as plant height, stem circumference, leaf number, leaf length and width of the control A are lower than those of the other treatments, the overall agronomic performance is slightly poor, and the difference between the two treatments applying the functional microbial agent is not large.
The agronomic performance survey results after capping for each treatment are shown in table 4. As can be seen from Table 4, the agronomic performance indexes such as stem circumference, waist leaf width and apical leaf width No. 3 of treatment A are lower than those of the other treatments, the overall agronomic performance is slightly poor, and the difference between the two treatments applying the functional microbial agent is not large.
TABLE 3 flourishing Long-term agronomic traits
TABLE 4 agronomic traits after capping
3. Root system weight
The average root weights for the different treatments after harvest and baking are shown in table 5. As can be seen from the table, the application of the functional microbial agent can obviously increase the weight of the root system of the tobacco plant, and compared with the control of the treatment A, the fresh weight of the root system of the treatment B and the fresh weight of the root system of the treatment C are respectively increased by 32.59 percent and 39.28 percent, and the dry weight of the root system is respectively increased by 27.10 percent and 38.85 percent.
TABLE 5 weight of roots treated differently
| Treatment of | Fresh weight of root (g) | Root weight (g) |
| A | 472.0 | 149.8 |
| B | 625.8 | 190.4 |
| C | 657.4 | 208.0 |
4. Economic character
As can be seen from Table 6, the yield per mu and the average price are the highest for treatment C, and the yield per mu and the average price are lower for treatment A after treatment B; the first-class smoke proportion of each treatment is sequentially from high to low, namely treatment B, treatment C and treatment A; the middle-to-high smoke proportion of each treatment is sequentially from high to low, namely treatment C > treatment B > treatment A.
TABLE 6 statistical table of economic traits
Third, conclusion and discussion
The test result in Eden in 2020 shows that the application of the functional microbial inoculum in the embodiment 1 and the embodiment 2 can promote the early growth and the fast growth of tobacco plants, so that the tobacco plants can enter the agglomeration period and the vigorous growth period in advance; under the condition that rainfall is increased during mature harvest, the nutrient tolerance of the flue-cured tobacco can be improved by applying the functional microbial agent.
The application of the functional microbial agent can promote the growth of the root system of the tobacco plant and obviously improve the weight of the root system of the tobacco plant; the stem circumference of the tobacco plant can be increased, the leaf opening is promoted, and the agronomic characters of the flue-cured tobacco are improved; can improve the yield per mu and the value per mu of the flue-cured tobacco, and improve the first-class tobacco proportion and the middle-class tobacco proportion of the flue-cured tobacco.
The above description is only for the specific embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art can easily conceive of the changes or substitutions within the technical scope of the present invention, and all the changes or substitutions should be covered within the scope of the present invention. Therefore, the protection scope of the present invention shall be subject to the protection scope of the appended claims.
Claims (10)
1. A microbial agent for promoting the early growth and the quick growth of tobacco plants is characterized by comprising the following components in percentage by mass: 1 to 2 percent of compound microorganism bacterium powder, 1.8 to 4.6 percent of biostimulant and 93.4 to 97.2 percent of carrier, wherein the sum of the mass percentages of the components is 100 percent; wherein the content of the first and second substances,
the compound microbial powder is prepared by compounding a strain WSWFJ39, a strain WSWFJ41 and a strain WSWFJ 46; the strain WSWFJ39 is bacillus amyloliquefaciens with the preservation number of CGMCC No.20147, the strain WSWFJ41 is bacillus with the preservation number of CGMCC No.20151, and the strain WSWFJ46 is bacillus subtilis with the preservation number of CGMCC No. 20145;
the biological stimulin is one or more of mineral fulvic acid, chitin and polyglutamic acid;
the carrier is an organic fertilizer.
2. The microbial agent for promoting the early growth and the quick growth of tobacco strains according to claim 1, wherein the effective viable count of the compound microbial powder is more than 1.0 x 109cfu/g。
3. The microbial agent for promoting the early growth and quick growth of tobacco strains according to claim 1 or 2, wherein the effective viable bacteria number ratio of the strain WSWJ39, the strain WSWJ41 and the strain WSWJ46 in the compound microbial powder is 2: 1.
4. The microbial agent for promoting the early growth and the quick growth of tobacco strains according to claim 1, wherein the biostimulant comprises the following components in percentage by mass: 1 to 3 percent of mineral source fulvic acid, 0.3 to 0.6 percent of chitin and 0.5 to 1 percent of polyglutamic acid.
5. The microbial agent for promoting the early growth and the quick growth of tobacco strains according to claim 1, wherein the carrier is an organic fertilizer fermented by pure rapeseed cake, and the content of organic matters in the carrier is not less than 80%, and the content of total nutrients in the carrier is not less than 8.0%.
6. A preparation method of a microbial agent for promoting the early growth and the quick growth of tobacco plants is characterized by comprising the following steps:
s1: compounding compound microbial bacteria powder, namely, respectively detecting the viable count of the bacterial powder of the bacterial strain WSWFJ39, the bacterial strain WSWFJ41 and the bacterial powder of the bacterial strain WSWFJ46, and then compounding the bacterial powder according to the ratio of the effective viable count to the effective viable count of 2: 1;
s2: mixing the biological stimulin with the composite microbial powder, and uniformly mixing the composite microbial powder obtained by compounding the S1 with one or more of mineral fulvic acid, chitin and polyglutamic acid;
s3: preparing a microbial agent for promoting the early growth and the quick growth of tobacco plants, namely adding the mixture of the biostimulant obtained in the step S2 and the composite microbial powder into a carrier organic fertilizer, fully and uniformly mixing, and extruding and granulating to obtain the microbial agent for promoting the early growth and the quick growth of the tobacco plants;
wherein the composite microbial powder, the biological stimulin and the carrier respectively comprise the following components in percentage by mass: 1-2% of composite microbial powder, 1.8-4.6% of biological stimulin and 93.4-97.2% of carrier, wherein the sum of the mass percentages of the components is 100%;
wherein the strain WSWFJ39 in the step S1 is Bacillus amyloliquefaciens with the preservation number of CGMCC No.20147, the strain WSWFJ41 is Bacillus with the preservation number of CGMCC No.20151, and the strain WSWFJ46 is Bacillus subtilis with the preservation number of CGMCC No. 20145.
7. The method for preparing the microbial agent for promoting the early growth and fast hair of tobacco strains according to claim 6, wherein in the step S1, the effective viable count of the compound microbial powder is more than 1.0 x 109cfu/g。
8. The method for preparing a microbial agent for promoting the early growth and fast hair of tobacco strains according to claim 6, wherein in the step S2, the biostimulant comprises mineral fulvic acid, chitin and polyglutamic acid, and the mass percentages of the components are as follows: 1 to 3 percent of mineral source fulvic acid, 0.3 to 0.6 percent of chitin and 0.5 to 1 percent of polyglutamic acid.
9. The method for preparing the microbial agent for promoting the early growth and the quick growth of tobacco strains according to claim 6, wherein in the step S3, the carrier organic fertilizer is an organic fertilizer obtained by fermenting pure rapeseed cake, and the carrier contains not less than 80% of organic matters and not less than 8.0% of total nutrients.
10. Use of the microbial agent for promoting the early growth and fast growth of tobacco strains according to any one of claims 1 to 5 in tobacco planting.
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