LV10307B - Molecular cloning and expression of genes encoding proteolytic enzymes - Google Patents

Molecular cloning and expression of genes encoding proteolytic enzymes Download PDF

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Publication number
LV10307B
LV10307B LVP-93-1390A LV931390A LV10307B LV 10307 B LV10307 B LV 10307B LV 931390 A LV931390 A LV 931390A LV 10307 B LV10307 B LV 10307B
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LV
Latvia
Prior art keywords
bacillus
host cell
dna
cell
serine protease
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Application number
LVP-93-1390A
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English (en)
Latvian (lv)
Other versions
LV10307A (lv
Inventor
Eekelen Christiaan A G Van
Der Laan Johannes C Van
Leonardus J S M Mulleners
Original Assignee
Gist Brocades Nv
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Gist Brocades Nv filed Critical Gist Brocades Nv
Publication of LV10307A publication Critical patent/LV10307A/xx
Publication of LV10307B publication Critical patent/LV10307B/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
LVP-93-1390A 1987-02-27 1993-12-29 Molecular cloning and expression of genes encoding proteolytic enzymes LV10307B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP87200358 1987-02-27
PCT/NL1988/000007 WO1988006624A2 (en) 1987-02-27 1988-02-26 Molecular cloning and expression of genes encoding proteolytic enzymes

Publications (2)

Publication Number Publication Date
LV10307A LV10307A (lv) 1994-10-20
LV10307B true LV10307B (en) 1995-08-20

Family

ID=8197584

Family Applications (1)

Application Number Title Priority Date Filing Date
LVP-93-1390A LV10307B (en) 1987-02-27 1993-12-29 Molecular cloning and expression of genes encoding proteolytic enzymes

Country Status (19)

Country Link
US (1) US5217878A (lt)
JP (1) JP3026567B2 (lt)
KR (1) KR0127560B1 (lt)
CN (1) CN88101681A (lt)
AT (2) ATE181110T1 (lt)
AU (1) AU620026B2 (lt)
BG (1) BG49049A3 (lt)
BR (1) BR8805647A (lt)
CA (1) CA1339100C (lt)
DE (2) DE3856331T2 (lt)
DK (2) DK175738B1 (lt)
FI (3) FI105483B (lt)
HU (1) HU213220B (lt)
LT (1) LT4000B (lt)
LV (1) LV10307B (lt)
NO (1) NO884423D0 (lt)
PT (1) PT86864B (lt)
RU (1) RU2023723C1 (lt)
WO (1) WO1988006624A2 (lt)

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DK6488D0 (da) * 1988-01-07 1988-01-07 Novo Industri As Enzymer
GB2214508A (en) * 1988-01-27 1989-09-06 Bayer Ag Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences.
US6287841B1 (en) * 1988-02-11 2001-09-11 Genencor International, Inc. High alkaline serine protease
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US5665587A (en) * 1989-06-26 1997-09-09 Novo Nordisk A/S Modified subtilisins and detergent compositions containing same
JP3046344B2 (ja) * 1990-10-24 2000-05-29 塩野義製薬株式会社 新規プロテアーゼ
US5482849A (en) * 1990-12-21 1996-01-09 Novo Nordisk A/S Subtilisin mutants
DK58391D0 (da) * 1991-04-03 1991-04-03 Novo Nordisk As Hidtil ukendte proteaser
DE69333463D1 (de) * 1992-05-18 2004-05-06 Genencor Int Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen
US6440717B1 (en) * 1993-09-15 2002-08-27 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis
US6436690B1 (en) * 1993-09-15 2002-08-20 The Procter & Gamble Company BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted
US6599730B1 (en) * 1994-05-02 2003-07-29 Procter & Gamble Company Subtilisin 309 variants having decreased adsorption and increased hydrolysis
US5831004A (en) 1994-10-27 1998-11-03 Affymax Technologies N.V. Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use
US5646044A (en) * 1995-03-02 1997-07-08 Henkel Kommanditgesellschaft Auf Aktien Expression systems for the production of target proteins in bacillus
US6455295B1 (en) * 1995-03-08 2002-09-24 The Procter & Gamble Company Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis
US6475765B1 (en) * 1995-03-09 2002-11-05 Procter & Gamble Company Subtilisin DY variants having decreased adsorption and increased hydrolysis
IL117350A0 (en) * 1995-03-09 1996-07-23 Procter & Gamble Proteinase k variants having decreased adsorption and increased hydrolysis
CN1530443A (zh) 1996-11-04 2004-09-22 ŵ����÷�����޹�˾ 枯草杆菌酶变异体和组合物
US5766871A (en) * 1996-11-27 1998-06-16 Food Industry Research And Development Institute Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase
US6420157B1 (en) * 1999-04-30 2002-07-16 Ortho-Mcneil Pharmaceutical, Inc. Zymogen activation system
US6485957B1 (en) * 1999-04-30 2002-11-26 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease EOS
US6458564B1 (en) * 1999-08-31 2002-10-01 Ortho-Mcneil Pharmaceutical, Inc. DNA encoding the human serine protease T
US6426199B1 (en) 1999-08-31 2002-07-30 Ortho-Mcneil Pharmaceutical, Inc. Dna
CN1312370A (zh) * 2000-03-07 2001-09-12 上海博德基因开发有限公司 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸
US6777218B1 (en) * 2000-03-14 2004-08-17 Novozymes A/S Subtilase enzymes having an improved wash performance on egg stains
DE60144145D1 (de) * 2000-04-03 2011-04-14 Maxygen Inc Subtilisin-variante
DK2339016T3 (da) 2002-03-29 2017-01-23 Danisco Us Inc Øget produktion af subtilisiner
EP1625217B1 (en) * 2003-05-12 2014-12-17 Danisco US Inc. Novel lipolytic enzyme elip
EP1625202A4 (en) 2003-05-12 2010-10-20 Genencor Int NEW LIPOLYTIC ENZYME LIP1
EP1625208A4 (en) * 2003-05-12 2006-10-18 Genencor Int NEW LIPOLYTIC ENZYME LIP2
AU2004277913B2 (en) 2003-09-19 2010-04-08 Bakery Technology Centre Bv Preparation of an edible product from dough
EP1694847B1 (en) * 2003-11-19 2012-06-13 Danisco US Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
EP1735339A2 (en) * 2004-04-09 2006-12-27 Genencor International, Inc. Pcka modifications and enhanced protein expression in bacillus
US20080044853A1 (en) * 2004-06-21 2008-02-21 Novozymes A/S Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation
US7202074B2 (en) * 2004-07-22 2007-04-10 University Of Chile Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme
EP2129779B2 (en) 2007-03-12 2018-12-26 Danisco US Inc. Modified proteases
US8343735B2 (en) * 2007-05-10 2013-01-01 Danisco Us Inc. Modified secretion system to increase expression of polypeptides in bacteria
ES2562919T3 (es) 2007-08-06 2016-03-09 University Of Chile Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío
RU2515112C2 (ru) 2007-12-21 2014-05-10 ДАНИСКО ЮЭс ИНК. Улучшенная продукция белка в bacillus
FI123122B (fi) 2009-02-16 2012-11-15 Optogear Oy Laitteisto lasimateriaalin valmistamiseksi
WO2010123754A1 (en) 2009-04-24 2010-10-28 Danisco Us Inc. Proteases with modified pro regions
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
EP2751266B1 (en) * 2011-09-22 2017-03-29 Novozymes A/S Polypeptides having protease activity and polynucleotides encoding same
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Also Published As

Publication number Publication date
DK593988D0 (da) 1988-10-26
DE3856331T2 (de) 1999-10-14
ATE181110T1 (de) 1999-06-15
DK200401262A (da) 2004-08-20
FI884904A (fi) 1988-10-24
BR8805647A (pt) 1989-10-31
KR0127560B1 (ko) 1997-12-29
KR890700665A (ko) 1989-04-26
LV10307A (lv) 1994-10-20
PT86864A (pt) 1988-03-01
BG49049A3 (en) 1991-07-15
FI20001152A (fi) 2000-05-15
WO1988006624A2 (en) 1988-09-07
CN88101681A (zh) 1988-11-09
FI925413A (fi) 1992-11-27
FI925413A0 (fi) 1992-11-27
DK593988A (da) 1988-10-26
NO884423L (no) 1988-10-05
DE3856339D1 (de) 1999-07-15
DE3856339T2 (de) 1999-11-04
AU1398088A (en) 1988-09-26
AU620026B2 (en) 1992-02-13
DK175738B1 (da) 2005-02-07
DK175852B1 (da) 2005-04-04
JPH01502876A (ja) 1989-10-05
WO1988006624A3 (en) 1988-11-17
ATE180016T1 (de) 1999-05-15
HU213220B (en) 1997-03-28
FI119028B (fi) 2008-06-30
US5217878A (en) 1993-06-08
CA1339100C (en) 1997-07-29
JP3026567B2 (ja) 2000-03-27
FI105483B (fi) 2000-08-31
FI106726B (fi) 2001-03-30
LTIP1820A (en) 1995-08-25
NO884423D0 (no) 1988-10-05
LT4000B (en) 1996-06-25
PT86864B (pt) 1992-05-29
RU2023723C1 (ru) 1994-11-30
FI884904A0 (fi) 1988-10-24
DE3856331D1 (de) 1999-06-17

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