HU213220B - Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells - Google Patents
Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells Download PDFInfo
- Publication number
- HU213220B HU213220B HU881832A HU183288A HU213220B HU 213220 B HU213220 B HU 213220B HU 881832 A HU881832 A HU 881832A HU 183288 A HU183288 A HU 183288A HU 213220 B HU213220 B HU 213220B
- Authority
- HU
- Hungary
- Prior art keywords
- bacillus
- cells
- dna
- serine protease
- protoplasts
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
- C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
Landscapes
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Mycology (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Peptides Or Proteins (AREA)
- Immobilizing And Processing Of Enzymes And Microorganisms (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP87200358 | 1987-02-27 |
Publications (1)
Publication Number | Publication Date |
---|---|
HU213220B true HU213220B (en) | 1997-03-28 |
Family
ID=8197584
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
HU881832A HU213220B (en) | 1987-02-27 | 1988-02-26 | Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells |
Country Status (19)
Country | Link |
---|---|
US (1) | US5217878A (no) |
JP (1) | JP3026567B2 (no) |
KR (1) | KR0127560B1 (no) |
CN (1) | CN88101681A (no) |
AT (2) | ATE181110T1 (no) |
AU (1) | AU620026B2 (no) |
BG (1) | BG49049A3 (no) |
BR (1) | BR8805647A (no) |
CA (1) | CA1339100C (no) |
DE (2) | DE3856331T2 (no) |
DK (2) | DK175738B1 (no) |
FI (3) | FI105483B (no) |
HU (1) | HU213220B (no) |
LT (1) | LT4000B (no) |
LV (1) | LV10307B (no) |
NO (1) | NO884423D0 (no) |
PT (1) | PT86864B (no) |
RU (1) | RU2023723C1 (no) |
WO (1) | WO1988006624A2 (no) |
Families Citing this family (50)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
KR0127560B1 (ko) * | 1987-02-27 | 1997-12-29 | 마가렛트 에이.호른 | 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현 |
DK6488D0 (da) * | 1988-01-07 | 1988-01-07 | Novo Industri As | Enzymer |
GB2214508A (en) * | 1988-01-27 | 1989-09-06 | Bayer Ag | Plasmid vector for the efficient expression of foreign genes fused with newly conceived transcriptional and translational signal sequences. |
US6287841B1 (en) * | 1988-02-11 | 2001-09-11 | Genencor International, Inc. | High alkaline serine protease |
AU622916B2 (en) * | 1988-05-05 | 1992-04-30 | Applied Microbiology, Inc | Protease-deficient gram-positive bacteria and their use as host organisms for the production of recombinant products |
US5665587A (en) * | 1989-06-26 | 1997-09-09 | Novo Nordisk A/S | Modified subtilisins and detergent compositions containing same |
JP3046344B2 (ja) * | 1990-10-24 | 2000-05-29 | 塩野義製薬株式会社 | 新規プロテアーゼ |
US5482849A (en) * | 1990-12-21 | 1996-01-09 | Novo Nordisk A/S | Subtilisin mutants |
DK58391D0 (da) * | 1991-04-03 | 1991-04-03 | Novo Nordisk As | Hidtil ukendte proteaser |
DE69333463D1 (de) * | 1992-05-18 | 2004-05-06 | Genencor Int | Alkalische Proteasen produzierende Bakterien, und Verfahren zur Herstellung dieser alkalischen Proteasen |
US6440717B1 (en) * | 1993-09-15 | 2002-08-27 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis |
US6436690B1 (en) * | 1993-09-15 | 2002-08-20 | The Procter & Gamble Company | BPN′ variants having decreased adsorption and increased hydrolysis wherein one or more loop regions are substituted |
US6599730B1 (en) * | 1994-05-02 | 2003-07-29 | Procter & Gamble Company | Subtilisin 309 variants having decreased adsorption and increased hydrolysis |
US5831004A (en) | 1994-10-27 | 1998-11-03 | Affymax Technologies N.V. | Inhibitors of metalloproteases, pharmaceutical compositions comprising same and methods of their use |
US5646044A (en) * | 1995-03-02 | 1997-07-08 | Henkel Kommanditgesellschaft Auf Aktien | Expression systems for the production of target proteins in bacillus |
US6455295B1 (en) * | 1995-03-08 | 2002-09-24 | The Procter & Gamble Company | Subtilisin Carlsberg variants having decreased adsorption and increased hydrolysis |
US6475765B1 (en) * | 1995-03-09 | 2002-11-05 | Procter & Gamble Company | Subtilisin DY variants having decreased adsorption and increased hydrolysis |
IL117350A0 (en) * | 1995-03-09 | 1996-07-23 | Procter & Gamble | Proteinase k variants having decreased adsorption and increased hydrolysis |
CN1530443A (zh) | 1996-11-04 | 2004-09-22 | ŵ����÷������˾ | 枯草杆菌酶变异体和组合物 |
US5766871A (en) * | 1996-11-27 | 1998-06-16 | Food Industry Research And Development Institute | Screening and characterization of glutaryl-7-aminocephalosporanic acid acylase |
US6420157B1 (en) * | 1999-04-30 | 2002-07-16 | Ortho-Mcneil Pharmaceutical, Inc. | Zymogen activation system |
US6485957B1 (en) * | 1999-04-30 | 2002-11-26 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding the human serine protease EOS |
US6458564B1 (en) * | 1999-08-31 | 2002-10-01 | Ortho-Mcneil Pharmaceutical, Inc. | DNA encoding the human serine protease T |
US6426199B1 (en) | 1999-08-31 | 2002-07-30 | Ortho-Mcneil Pharmaceutical, Inc. | Dna |
CN1312370A (zh) * | 2000-03-07 | 2001-09-12 | 上海博德基因开发有限公司 | 一种新的多肽——人atp依赖的丝氨酸蛋白水解酶11和编码这种多肽的多核苷酸 |
US6777218B1 (en) * | 2000-03-14 | 2004-08-17 | Novozymes A/S | Subtilase enzymes having an improved wash performance on egg stains |
DE60144145D1 (de) * | 2000-04-03 | 2011-04-14 | Maxygen Inc | Subtilisin-variante |
DK2339016T3 (da) | 2002-03-29 | 2017-01-23 | Danisco Us Inc | Øget produktion af subtilisiner |
EP1625217B1 (en) * | 2003-05-12 | 2014-12-17 | Danisco US Inc. | Novel lipolytic enzyme elip |
EP1625202A4 (en) | 2003-05-12 | 2010-10-20 | Genencor Int | NEW LIPOLYTIC ENZYME LIP1 |
EP1625208A4 (en) * | 2003-05-12 | 2006-10-18 | Genencor Int | NEW LIPOLYTIC ENZYME LIP2 |
AU2004277913B2 (en) | 2003-09-19 | 2010-04-08 | Bakery Technology Centre Bv | Preparation of an edible product from dough |
EP1694847B1 (en) * | 2003-11-19 | 2012-06-13 | Danisco US Inc. | Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same |
US7985569B2 (en) | 2003-11-19 | 2011-07-26 | Danisco Us Inc. | Cellulomonas 69B4 serine protease variants |
EP1735339A2 (en) * | 2004-04-09 | 2006-12-27 | Genencor International, Inc. | Pcka modifications and enhanced protein expression in bacillus |
US20080044853A1 (en) * | 2004-06-21 | 2008-02-21 | Novozymes A/S | Stably Maintained Multiple Copies of at Least Two Orf in the Same Orientation |
US7202074B2 (en) * | 2004-07-22 | 2007-04-10 | University Of Chile | Protein and nucleic acid sequence encoding a KRILL-derived cold adapted trypsin-like activity enzyme |
EP2129779B2 (en) | 2007-03-12 | 2018-12-26 | Danisco US Inc. | Modified proteases |
US8343735B2 (en) * | 2007-05-10 | 2013-01-01 | Danisco Us Inc. | Modified secretion system to increase expression of polypeptides in bacteria |
ES2562919T3 (es) | 2007-08-06 | 2016-03-09 | University Of Chile | Proteína y secuencia de ADN que codifica una actividad del tipo de la subtilisina adaptada al frío |
RU2515112C2 (ru) | 2007-12-21 | 2014-05-10 | ДАНИСКО ЮЭс ИНК. | Улучшенная продукция белка в bacillus |
FI123122B (fi) | 2009-02-16 | 2012-11-15 | Optogear Oy | Laitteisto lasimateriaalin valmistamiseksi |
WO2010123754A1 (en) | 2009-04-24 | 2010-10-28 | Danisco Us Inc. | Proteases with modified pro regions |
AR076941A1 (es) | 2009-06-11 | 2011-07-20 | Danisco Us Inc | Cepa de bacillus para una mayor produccion de proteina |
EP2751266B1 (en) * | 2011-09-22 | 2017-03-29 | Novozymes A/S | Polypeptides having protease activity and polynucleotides encoding same |
CN103013960A (zh) * | 2012-12-21 | 2013-04-03 | 青岛蔚蓝生物集团有限公司 | 一种碱性蛋白酶及其重组表达工程菌 |
MX2016008595A (es) | 2013-12-31 | 2016-10-28 | Danisco Us Inc | Expresion mejorada de proteínas. |
KR102500121B1 (ko) | 2014-12-16 | 2023-02-20 | 다니스코 유에스 인크. | 향상된 단백질 발현 |
EP3234149A1 (en) | 2014-12-19 | 2017-10-25 | Danisco US Inc. | Enhanced protein expression |
EP3619309B1 (en) * | 2017-05-05 | 2024-04-10 | Bioecho Life Sciences GmbH | Rapid purification of high quality nucleic acids from biological samples |
Family Cites Families (16)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
GB1234445A (no) | 1967-10-03 | 1971-06-03 | ||
GB1205403A (en) | 1967-11-10 | 1970-09-16 | Novo Terapeutisk Labor As | Preparation of proteolytic enzyme preparations |
GB1519148A (en) * | 1974-11-19 | 1978-07-26 | Gist Brocades Nv | Compositions of matter |
FR2482980A1 (fr) | 1980-05-20 | 1981-11-27 | Biomerieux Sa | Procede d'obtention de preparations de toxoplasmes pour le diagnostic de la toxoplasmose, et preparations ainsi obtenues |
JPS6055118B2 (ja) | 1982-02-08 | 1985-12-03 | 昭和電工株式会社 | 新規な細菌アルカリプロテア−ゼ及びその製造方法 |
DE3380878D1 (en) * | 1982-11-01 | 1989-12-28 | Miles Inc | Method of heterologous cloning of gene in bacillus microorganism |
NZ208612A (en) * | 1983-06-24 | 1991-09-25 | Genentech Inc | Method of producing "procaryotic carbonyl hydrolases" containing predetermined, site specific mutations |
DE3480411D1 (en) * | 1983-07-06 | 1989-12-14 | Gist Brocades Nv | Molecular cloning and expression in industrial microorganism species |
EP0138075A1 (en) * | 1983-09-16 | 1985-04-24 | Synergen Associates, Inc. | A method for the generation and detection of enzyme variants with enhanced themostability and activity |
US4828994A (en) * | 1984-09-21 | 1989-05-09 | Genex Corporation | Bacillus strains with reduced extracellular protease levels |
FR2582316B1 (fr) * | 1985-05-22 | 1988-12-02 | Centre Nat Rech Scient | Vecteurs d'expression de l'a-amylase dans bacillus subtilis, souches obtenues et procede de preparation d'a-amylase |
DE3527913A1 (de) * | 1985-08-03 | 1987-02-12 | Henkel Kgaa | Alkalische protease, verfahren zur herstellung von hybridvektoren und genetisch transformierte mikroorganismen |
JP2599946B2 (ja) * | 1986-01-15 | 1997-04-16 | アムジェン,インコーポレイテツド | ズブチリシン類似体 |
ATE116365T1 (de) * | 1986-04-30 | 1995-01-15 | Genencor Int | Mutante einer nicht menschlichen carbonyl- hydrolase, für diese kodierende dns-sequenzen und vektoren und durch diese vektoren transformierte wirte. |
KR0127560B1 (ko) * | 1987-02-27 | 1997-12-29 | 마가렛트 에이.호른 | 단백질 분해 효소 코드화 유전자의 분자클로닝 및 발현 |
ES2135386T3 (es) * | 1987-02-27 | 1999-11-01 | Genencor Int | Transformacion de cepas de bacillus alcalofilas. |
-
1988
- 1988-02-26 KR KR1019880701347A patent/KR0127560B1/ko not_active IP Right Cessation
- 1988-02-26 PT PT86864A patent/PT86864B/pt not_active IP Right Cessation
- 1988-02-26 WO PCT/NL1988/000007 patent/WO1988006624A2/en active IP Right Grant
- 1988-02-26 JP JP63502472A patent/JP3026567B2/ja not_active Expired - Lifetime
- 1988-02-26 AU AU13980/88A patent/AU620026B2/en not_active Expired
- 1988-02-26 BR BR888805647A patent/BR8805647A/pt not_active IP Right Cessation
- 1988-02-26 HU HU881832A patent/HU213220B/hu not_active IP Right Cessation
- 1988-02-27 CN CN198888101681A patent/CN88101681A/zh active Pending
- 1988-02-29 DE DE3856331T patent/DE3856331T2/de not_active Expired - Lifetime
- 1988-02-29 AT AT91203297T patent/ATE181110T1/de not_active IP Right Cessation
- 1988-02-29 DE DE3856339T patent/DE3856339T2/de not_active Expired - Lifetime
- 1988-02-29 US US07/162,184 patent/US5217878A/en not_active Expired - Lifetime
- 1988-02-29 AT AT88200377T patent/ATE180016T1/de not_active IP Right Cessation
- 1988-02-29 CA CA000560126A patent/CA1339100C/en not_active Expired - Lifetime
- 1988-10-05 NO NO884423A patent/NO884423D0/no unknown
- 1988-10-24 FI FI884904A patent/FI105483B/fi active IP Right Grant
- 1988-10-25 BG BG085831A patent/BG49049A3/xx unknown
- 1988-10-26 DK DK198805939A patent/DK175738B1/da not_active IP Right Cessation
- 1988-10-26 RU SU884356848A patent/RU2023723C1/ru active
-
1992
- 1992-11-27 FI FI925413A patent/FI106726B/fi active
-
1993
- 1993-12-29 LV LVP-93-1390A patent/LV10307B/en unknown
-
1994
- 1994-01-28 LT LTIP1820A patent/LT4000B/lt not_active IP Right Cessation
-
2000
- 2000-05-15 FI FI20001152A patent/FI119028B/fi not_active IP Right Cessation
-
2004
- 2004-08-20 DK DK200401262A patent/DK175852B1/da not_active IP Right Cessation
Also Published As
Similar Documents
Publication | Publication Date | Title |
---|---|---|
HU213220B (en) | Method for enhanced production of serine protease in transformed bacillus cells and production of the transformed cells | |
EP0283075B1 (en) | Molecular cloning and expression of genes encoding proteolytic enzymes | |
US5733723A (en) | Stable gene amplification in chromosomal DNA of prokaryotic microorganisms | |
FI85287B (fi) | Molekulaer kloning och expression i industriella mikro-organismarter. | |
EP0127328A2 (en) | The use of chromosomal integration to stabilize heterologous genes | |
JP2001514529A (ja) | プロテアーゼ活性を有するポリペプチドをコードする核酸 | |
RU2060276C1 (ru) | Способ получения алкалофильного штамма бацилл с пониженным уровнем внеклеточной щелочной протеазы, способ получения высокощелочной протеазы и мутантная форма высокощелочной протеазы | |
EP0303668A1 (en) | Stable expression vectors |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
HPC4 | Succession in title of patentee |
Owner name: GENENCOR INTERNATIONAL INC., US |
|
HMM4 | Cancellation of final prot. due to non-payment of fee |