DE69328519T2 - Chondroitinase, Herstellungsverfahren und pharmazeutische Zusammensetzung - Google Patents

Chondroitinase, Herstellungsverfahren und pharmazeutische Zusammensetzung

Info

Publication number
DE69328519T2
DE69328519T2 DE69328519T DE69328519T DE69328519T2 DE 69328519 T2 DE69328519 T2 DE 69328519T2 DE 69328519 T DE69328519 T DE 69328519T DE 69328519 T DE69328519 T DE 69328519T DE 69328519 T2 DE69328519 T2 DE 69328519T2
Authority
DE
Germany
Prior art keywords
chondroitinase
chondroitinase abc
crystallizable
sds
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69328519T
Other languages
English (en)
Other versions
DE69328519D1 (de
Inventor
Nobukazu Hashimoto
Akio Hamai
Hideo Mochizuki
Shinichi Ishikawa
Misako Torikai
Isao Miyachi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Application granted granted Critical
Publication of DE69328519D1 publication Critical patent/DE69328519D1/de
Publication of DE69328519T2 publication Critical patent/DE69328519T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2063—Proteins, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C1/00—Photosensitive materials
    • G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
    • G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
    • G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
    • G03C2001/0471—Isoelectric point of gelatine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
DE69328519T 1992-06-26 1993-06-25 Chondroitinase, Herstellungsverfahren und pharmazeutische Zusammensetzung Expired - Lifetime DE69328519T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP19288292 1992-06-26
JP31098092A JP3895782B2 (ja) 1992-10-26 1992-10-26 コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (2)

Publication Number Publication Date
DE69328519D1 DE69328519D1 (de) 2000-06-08
DE69328519T2 true DE69328519T2 (de) 2000-09-14

Family

ID=26507572

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69328519T Expired - Lifetime DE69328519T2 (de) 1992-06-26 1993-06-25 Chondroitinase, Herstellungsverfahren und pharmazeutische Zusammensetzung

Country Status (10)

Country Link
US (3) US5496718A (de)
EP (1) EP0576294B1 (de)
KR (1) KR100280779B1 (de)
AT (1) ATE192486T1 (de)
CA (1) CA2099138C (de)
DE (1) DE69328519T2 (de)
DK (1) DK0576294T3 (de)
ES (1) ES2146600T3 (de)
RU (1) RU2144833C1 (de)
SG (1) SG50677A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
US7008783B1 (en) 1992-09-22 2006-03-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
JP3419811B2 (ja) * 1993-02-24 2003-06-23 マルハ株式会社 コンドロイチナーゼ遺伝子
CA2161125A1 (en) * 1993-04-23 1994-11-10 Michael Joseph Ryan Cloning and expression of the chondroitinase i and ii genes from p. vulgaris
WO1995013091A1 (en) * 1993-11-12 1995-05-18 International Technology Management Associates, Ltd. Methods of repairing connective tissues
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris
US5525500A (en) * 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
JPH10500843A (ja) * 1994-04-22 1998-01-27 アメリカン・サイアナミド・カンパニー コンドロイチナーゼｉおよびｉｉ、その製造法および用途
US5888798A (en) * 1995-06-07 1999-03-30 American Cyanamid Company Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
EP0747469A1 (de) * 1995-06-08 1996-12-11 The Procter & Gamble Company Chondroitinase enthaltende Reinigungszusammensetzungen
EP0875253B1 (de) * 1997-05-02 2005-04-06 Seikagaku Corporation Chondroitinase enthaltende Zusammensetzungen
JPH1149698A (ja) 1997-07-31 1999-02-23 Santen Pharmaceut Co Ltd 安定性を向上させたラクトフェリンの水性製剤
EP0900569B1 (de) * 1997-08-22 2002-10-02 Seikagaku Corporation Arzneimittel zur Behandlung von durch Hernie gestörter intervertebraler Bandscheibe
DE60038386T2 (de) * 1999-09-08 2009-04-23 Chugai Seiyaku K.K. Stabile proteinlösung abgefüllt in einem behältnis aus hydrophobem harz und eine methode zur stabilisierung derselben
WO2002099193A1 (fr) 2001-06-01 2002-12-12 Mitsubishi Paper Mills Limited Papier faisant intervenir un element d'echange thermique total
BR0211888A (pt) 2001-08-13 2004-08-24 Univ Florida Res Foudantion In Materiais e métodos para promoção de reparo de tecido nervoso
GB0205022D0 (en) 2002-03-04 2002-04-17 Univ Cambridge Tech Materials and methods for the treatment of cns damage
ATE500842T1 (de) 2002-05-04 2011-03-15 Acorda Therapeutics Inc Zusammensetzungen und verfahren zur förderung des neuronalen wachstums
AU2003243396A1 (en) * 2002-06-03 2003-12-19 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b
ES2366333T3 (es) * 2002-08-15 2011-10-19 Acorda Therapeutics, Inc. Proteína quimérica.
EP2298874A1 (de) * 2002-12-16 2011-03-23 Halozyme, Inc. Humanes Chondrotinaseglykoprotein (CHASEGP), Verfahren zu dessen Herstellung und pharmazeutische Zusammensetzungen, die dieses umfassen
PL1603541T5 (pl) * 2003-03-05 2013-06-28 Halozyme Inc Rozpuszczalna glikoproteina o aktywności hialuronidazy (sHASEGP), sposób jej wytwarzania, zastosowanie i zawierające ją kompozycje farmaceutyczne
US7871607B2 (en) * 2003-03-05 2011-01-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
US20060104968A1 (en) * 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
US20090123367A1 (en) * 2003-03-05 2009-05-14 Delfmems Soluble Glycosaminoglycanases and Methods of Preparing and Using Soluble Glycosaminoglycanases
US7959914B2 (en) 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
WO2004103299A2 (en) * 2003-05-16 2004-12-02 Acorda Therapeutics, Inc. Compositions and methods for the treatment of cns injuries
MX348062B (es) 2003-05-16 2017-05-26 Acorda Therapeutics Inc Mutantes que degradan proteoglicanos para tratamiento del snc.
GB0403247D0 (en) 2004-02-13 2004-03-17 Tillotts Pharma Ag A pharmaceutical composition
EP1737954A2 (de) * 2004-03-10 2007-01-03 The Massachusetts Institute Of Technology Rekombinante chondroitinase abc i und verwendungen davon
MXPA06013345A (es) * 2004-05-18 2008-10-31 Acorda Therapeutics Inc Metodos para purificar condrointinasa y sus formulaciones estables.
WO2005122734A2 (en) * 2004-06-14 2005-12-29 The Research Foundation Of State University Of New York Nanosphere/microsphere delivery system for the treatment of spinal cord injury
JP2008514240A (ja) * 2004-10-01 2008-05-08 インヴィトロジェン コーポレーション 生体分子のインビトロ合成用の供給バッファー、系、及び方法
EP1928490B8 (de) * 2005-09-26 2012-10-03 Acorda Therapeutics, Inc. Zusammensetzungen und verfahren zur verwendung von chondroitinase-abci-mutanten
WO2007063691A1 (ja) 2005-11-29 2007-06-07 Sanyo Chemical Industries, Ltd. 溶菌剤
AU2007307736C1 (en) 2006-10-10 2014-04-10 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase ABCI mutants
JP5341756B2 (ja) 2007-06-29 2013-11-13 国立大学法人名古屋大学 神経障害に基づく機能不全の改善剤およびＲｈｏキナーゼ活性化抑制剤
US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
US20130266555A1 (en) 2010-12-13 2013-10-10 Seikagaku Corporation Therapeutic agent for disc herniation
EP3199175B1 (de) 2014-09-27 2022-03-23 Sumitomo Dainippon Pharma Co., Ltd. Pharmazeutische zusammensetzung zur injektion
US9796970B1 (en) 2017-04-24 2017-10-24 Advantek Serum Laboratories Ltd. Production of high purity chondroitinase ABC
US11648299B2 (en) 2018-02-28 2023-05-16 Seikagaku Corporation Pharmaceutical composition, package and method for producing the same
CN114409731B (zh) * 2022-01-05 2023-08-11 浙江省农业科学院 同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549500A (en) * 1967-07-22 1970-12-22 Seikagaku Kogyo Co Ltd Novel chondroitinase and method of its recovery
JPS4832351B1 (de) * 1968-06-12 1973-10-05
JPS4713704Y1 (de) * 1971-09-29 1972-05-18
JPS6031478B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｂの製造法
JPS6031479B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｃの製造法
DK151634C (da) * 1978-09-29 1988-06-20 Mitsubishi Chem Ind Fremgangsmaade til rensning af langkaedet acyl-coenzym-a-syntetase
CA1221328A (en) * 1983-04-21 1987-05-05 Robert K. Scopes Enzyme extraction and purification processes
US4696816A (en) * 1985-11-07 1987-09-29 Brown Mark D Method for treating intervertebral disc displacement with enzymes
JPS62122588A (ja) * 1985-11-22 1987-06-03 Taiyo Fishery Co Ltd 純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JP2919496B2 (ja) * 1989-08-24 1999-07-12 生化学工業株式会社 新規コンドロイチン硫酸分解酵素、それらを産生する微生物並びにそれらの製法
JP3140797B2 (ja) * 1991-04-26 2001-03-05 生化学工業株式会社 安定化コンドロイチナーゼａｂｃ、その保存方法及び治療剤
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896

Also Published As

Publication number Publication date
US6184023B1 (en) 2001-02-06
AU4146493A (en) 1994-01-06
DK0576294T3 (da) 2000-10-09
CA2099138A1 (en) 1993-12-27
EP0576294A2 (de) 1993-12-29
ES2146600T3 (es) 2000-08-16
RU2144833C1 (ru) 2000-01-27
KR940005793A (ko) 1994-03-22
DE69328519D1 (de) 2000-06-08
EP0576294A3 (en) 1994-08-17
AU669528B2 (en) 1996-06-13
US5496718A (en) 1996-03-05
ATE192486T1 (de) 2000-05-15
SG50677A1 (en) 1998-07-20
EP0576294B1 (de) 2000-05-03
US5763205A (en) 1998-06-09
KR100280779B1 (ko) 2001-02-01
CA2099138C (en) 1998-09-22

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Legal Events

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8328 Change in the person/name/address of the agent

Representative=s name: BOETERS & LIECK, 81541 MUENCHEN