ES2146600T3 - Condroitinasa, procedimiento para su preparacion y composicion farmaceutica. - Google Patents

Condroitinasa, procedimiento para su preparacion y composicion farmaceutica.

Info

Publication number
ES2146600T3
ES2146600T3 ES93304979T ES93304979T ES2146600T3 ES 2146600 T3 ES2146600 T3 ES 2146600T3 ES 93304979 T ES93304979 T ES 93304979T ES 93304979 T ES93304979 T ES 93304979T ES 2146600 T3 ES2146600 T3 ES 2146600T3
Authority
ES
Spain
Prior art keywords
chondroitinase
chondroitinase abc
crystallizable
sds
measurement
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93304979T
Other languages
English (en)
Inventor
Nobukazu Hashimoto
Akio Hamai
Hideo Mochizuki
Shinichi Ishikawa
Misako Torikai
Isao Miyachi
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Seikagaku Corp
Original Assignee
Seikagaku Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja
Application filed by Seikagaku Corp filed Critical Seikagaku Corp
Application granted granted Critical
Publication of ES2146600T3 publication Critical patent/ES2146600T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2063—Proteins, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C1/00—Photosensitive materials
    • G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
    • G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
    • G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
    • G03C2001/0471—Isoelectric point of gelatine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)

Abstract

UNA CONDROITINASA ABC PURIFICADA, CRISTALIZABLE QUE TIENE UN PESO MOLECULAR DE UNOS 100.000 DALTONS MEDIANTE LA MEDIDA DE LA SDS-ELECTROFORESIS DE GEL DE POLIACRILAMIDA (SDS-PAGE) Y LA MEDIDA MEDIANTE EL METODO DE CROMATOGRAFIA DE INFILTRACION SOBRE GEL, QUE TIENE ALANINA COMO EL AMINOACIDO N-TERMINAL Y PROLINA COMO EL AMINOACIDO CTERMINAL. UN PROCESO PARA LA PURIFICACION DE LA CONDROITINASA ABC PURIFICADA, CRISTALIZABLE QUE CONSISTE EN ELIMINAR EL ACIDO NUCLEICO DE UN EXTRACTO DE UNA SOLUCION SURFACTANTE OBTENIDO DE CELULAS DE MICROORGANISMOS QUE PRODUCEN CONDROITINASA ABC Y TRATARLA CROMATOGRAFICAMENTE MEDIANTE UNA ELUCION POR GRADIENTE DE CONCENTRACION UTILIZANDO UNA RESINA DE INTERCAMBIO DE CATIONES DEBIL O UNA RESINA DE INTERCAMBIO DE CATIONES FUERTE. UNA COMPOSICION QUE CONTIENE UNA CONDROITINASA Y ALBUMINA DE SUERO, GELATINA O UN SURFACTANTE NO IONICO.
ES93304979T 1992-06-26 1993-06-25 Condroitinasa, procedimiento para su preparacion y composicion farmaceutica. Expired - Lifetime ES2146600T3 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP19288292 1992-06-26
JP31098092A JP3895782B2 (ja) 1992-10-26 1992-10-26 コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (1)

Publication Number Publication Date
ES2146600T3 true ES2146600T3 (es) 2000-08-16

Family

ID=26507572

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93304979T Expired - Lifetime ES2146600T3 (es) 1992-06-26 1993-06-25 Condroitinasa, procedimiento para su preparacion y composicion farmaceutica.

Country Status (10)

Country Link
US (3) US5496718A (es)
EP (1) EP0576294B1 (es)
KR (1) KR100280779B1 (es)
AT (1) ATE192486T1 (es)
CA (1) CA2099138C (es)
DE (1) DE69328519T2 (es)
DK (1) DK0576294T3 (es)
ES (1) ES2146600T3 (es)
RU (1) RU2144833C1 (es)
SG (1) SG50677A1 (es)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
US7008783B1 (en) 1992-09-22 2006-03-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
JP3419811B2 (ja) * 1993-02-24 2003-06-23 マルハ株式会社 コンドロイチナーゼ遺伝子
JPH09500011A (ja) * 1993-04-23 1997-01-07 アメリカン サイアナミド カンパニー Ｐ．ブルガリスからのコンドロイチナーゼｉ及びｉｉ遺伝子のクローニング及び発現
WO1995013091A1 (en) * 1993-11-12 1995-05-18 International Technology Management Associates, Ltd. Methods of repairing connective tissues
CA2188159A1 (en) * 1994-04-22 1995-11-02 Kiran Manohar Khandke Chondroitinases i and ii, methods of preparation, and use thereof
US5525500A (en) * 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris
US5888798A (en) * 1995-06-07 1999-03-30 American Cyanamid Company Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
EP0747469A1 (en) 1995-06-08 1996-12-11 The Procter & Gamble Company Cleaning compositions comprising chondroitinase
DE69829605T2 (de) * 1997-05-02 2006-02-09 Seikagaku Corp. Chondroitinase enthaltende Zusammensetzungen
JPH1149698A (ja) 1997-07-31 1999-02-23 Santen Pharmaceut Co Ltd 安定性を向上させたラクトフェリンの水性製剤
DK0900569T3 (da) * 1997-08-22 2003-01-27 Seikagaku Kogyo Co Ltd Terapeutisk middel til behandling af herniaramt intervertebral diskus
ATE389414T1 (de) 1999-09-08 2008-04-15 Chugai Pharmaceutical Co Ltd Stabile proteinlösung abgefüllt in einem behältnis aus hydrophobem harz und eine methode zur stabilisierung derselben
AU2002304108B2 (en) 2001-06-01 2004-04-22 Mitsubishi Paper Mills Limited Total heat exchange element-use paper
ATE543509T1 (de) 2001-08-13 2012-02-15 Univ Florida Material und verfahren zur förderung der reparatur von nervengewebe
GB0205022D0 (en) 2002-03-04 2002-04-17 Univ Cambridge Tech Materials and methods for the treatment of cns damage
ES2362648T3 (es) 2002-05-04 2011-07-08 Acorda Therapeutics, Inc. Composiciones y métodos para promover la proyección neuronal.
CA2493509C (en) * 2002-06-03 2010-03-09 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b
DE60337009D1 (de) * 2002-08-15 2011-06-16 Acorda Therapeutics Inc Chimärisches protein
CA2508948A1 (en) * 2002-12-16 2004-07-15 Halozyme, Inc. Human chondroitinase glycoprotein (chasegp), process for preparing the same, and pharmaceutical compositions comprising thereof
US20060104968A1 (en) * 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
US20090123367A1 (en) * 2003-03-05 2009-05-14 Delfmems Soluble Glycosaminoglycanases and Methods of Preparing and Using Soluble Glycosaminoglycanases
PL2163643T3 (pl) * 2003-03-05 2015-06-30 Halozyme Inc Rozpuszczalna glikoproteina o aktywności hialuronidazy (sHASEGP), sposób jej wytwarzania, zastosowania oraz zawierające ją kompozycje farmaceutyczne
US7871607B2 (en) * 2003-03-05 2011-01-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
EP3514238B1 (en) 2003-05-16 2021-05-05 Acorda Therapeutics, Inc. Proteoglycan degrading mutants for treatment of cns
US7959914B2 (en) 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
MXPA05012305A (es) * 2003-05-16 2006-04-18 Acorda Therapeutics Inc Composiciones y metodos para el tratamiento de lesiones del snc.
GB0403247D0 (en) 2004-02-13 2004-03-17 Tillotts Pharma Ag A pharmaceutical composition
CA2558984A1 (en) * 2004-03-10 2005-09-22 Massachusetts Institute Of Technology Recombinant chondroitinase abc i and uses thereof
WO2005112986A2 (en) * 2004-05-18 2005-12-01 Acorda Therapeutics, Inc. Purifying chondroitinase and stable formulations thereof
WO2005122734A2 (en) * 2004-06-14 2005-12-29 The Research Foundation Of State University Of New York Nanosphere/microsphere delivery system for the treatment of spinal cord injury
JP2008514240A (ja) * 2004-10-01 2008-05-08 インヴィトロジェン コーポレーション 生体分子のインビトロ合成用の供給バッファー、系、及び方法
CA2623635C (en) 2005-09-26 2013-04-02 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
WO2007063691A1 (ja) 2005-11-29 2007-06-07 Sanyo Chemical Industries, Ltd. 溶菌剤
ES2473610T3 (es) 2006-10-10 2014-07-07 Acorda Therapeutics, Inc. Composiciones y métodos de uso de mutantes de condroitinasa ABCI
WO2009005033A1 (ja) 2007-06-29 2009-01-08 National University Corporation Nagoya University 神経障害に基づく機能不全の改善剤およびＲｈｏキナーゼ活性化抑制剤
US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
CN106975077A (zh) * 2010-12-13 2017-07-25 生化学工业株式会社 椎间盘突出治疗剂
DK3199175T3 (da) 2014-09-27 2022-06-13 Sumitomo Pharma Co Ltd Farmaceutisk sammensætning til injektion
US9796970B1 (en) 2017-04-24 2017-10-24 Advantek Serum Laboratories Ltd. Production of high purity chondroitinase ABC
CN111727050A (zh) 2018-02-28 2020-09-29 生化学工业株式会社 药物组合物、包装体及其制造方法
CN114409731B (zh) * 2022-01-05 2023-08-11 浙江省农业科学院 同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

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US3549500A (en) * 1967-07-22 1970-12-22 Seikagaku Kogyo Co Ltd Novel chondroitinase and method of its recovery
JPS4832351B1 (es) * 1968-06-12 1973-10-05
JPS4713704Y1 (es) * 1971-09-29 1972-05-18
JPS6031478B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｂの製造法
JPS6031479B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｃの製造法
DE2962521D1 (en) * 1978-09-29 1982-05-27 Mitsubishi Chem Ind Process for the purification of long-chain acyl-coenzyme-a synthetase and the enzyme, acyl-coa synthetase, purified thereby
US4623625A (en) * 1983-04-21 1986-11-18 La Trobe University Enzyme extraction and purification processes
US4696816A (en) * 1985-11-07 1987-09-29 Brown Mark D Method for treating intervertebral disc displacement with enzymes
JPS62122588A (ja) * 1985-11-22 1987-06-03 Taiyo Fishery Co Ltd 純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JP2919496B2 (ja) * 1989-08-24 1999-07-12 生化学工業株式会社 新規コンドロイチン硫酸分解酵素、それらを産生する微生物並びにそれらの製法
JP3140797B2 (ja) * 1991-04-26 2001-03-05 生化学工業株式会社 安定化コンドロイチナーゼａｂｃ、その保存方法及び治療剤
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896

Also Published As

Publication number Publication date
ATE192486T1 (de) 2000-05-15
KR940005793A (ko) 1994-03-22
DE69328519D1 (de) 2000-06-08
EP0576294A2 (en) 1993-12-29
AU4146493A (en) 1994-01-06
AU669528B2 (en) 1996-06-13
CA2099138C (en) 1998-09-22
CA2099138A1 (en) 1993-12-27
DK0576294T3 (da) 2000-10-09
DE69328519T2 (de) 2000-09-14
EP0576294B1 (en) 2000-05-03
US5496718A (en) 1996-03-05
SG50677A1 (en) 1998-07-20
US6184023B1 (en) 2001-02-06
US5763205A (en) 1998-06-09
EP0576294A3 (en) 1994-08-17
KR100280779B1 (ko) 2001-02-01
RU2144833C1 (ru) 2000-01-27

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