CN111394311B - 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 - Google Patents
一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 Download PDFInfo
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- CN111394311B CN111394311B CN202010310198.9A CN202010310198A CN111394311B CN 111394311 B CN111394311 B CN 111394311B CN 202010310198 A CN202010310198 A CN 202010310198A CN 111394311 B CN111394311 B CN 111394311B
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Abstract
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CN202010310198.9A CN111394311B (zh) | 2020-04-20 | 2020-04-20 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
KR1020217008536A KR102552191B1 (ko) | 2020-04-20 | 2020-05-25 | 중간엽 줄기세포가 각막 상피세포로의 분화를 유도하는 무혈청 완전 배지 |
EP20873344.4A EP4141109A1 (en) | 2020-04-20 | 2020-05-25 | Serum-free complete culture medium capable of inducing differentiation of mesenchymal stem cells from corneal epithelial cells |
PCT/CN2020/092071 WO2021212592A1 (zh) | 2020-04-20 | 2020-05-25 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
US17/283,491 US20220041982A1 (en) | 2020-04-20 | 2020-05-25 | A serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell |
JP2021516585A JP7239686B2 (ja) | 2020-04-20 | 2020-05-25 | 角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地 |
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CN202010310198.9A CN111394311B (zh) | 2020-04-20 | 2020-04-20 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
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CN111394311A CN111394311A (zh) | 2020-07-10 |
CN111394311B true CN111394311B (zh) | 2022-07-01 |
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US (1) | US20220041982A1 (zh) |
EP (1) | EP4141109A1 (zh) |
JP (1) | JP7239686B2 (zh) |
KR (1) | KR102552191B1 (zh) |
CN (1) | CN111394311B (zh) |
WO (1) | WO2021212592A1 (zh) |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPWO2010134619A1 (ja) * | 2009-05-18 | 2012-11-12 | 国立大学法人東北大学 | 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法 |
CN105062970A (zh) * | 2015-08-25 | 2015-11-18 | 北京瑞思德生物医学研究院 | 一种将间充质干细胞诱导成神经细胞的诱导剂及诱导分化完全培养基 |
CN105087466A (zh) * | 2015-08-28 | 2015-11-25 | 广州赛莱拉干细胞科技股份有限公司 | 诱导脐带间充质干细胞向角膜上皮细胞分化的培养基和方法 |
CN105176912A (zh) * | 2015-08-28 | 2015-12-23 | 广州赛莱拉干细胞科技股份有限公司 | 一种培养基及其应用、制备方法 |
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AU2003283004A1 (en) * | 2002-10-22 | 2004-05-13 | Waratah Pharmaceuticals, Inc. | Treatment of diabetes |
DK1789534T3 (en) * | 2004-08-16 | 2014-11-17 | Cellres Corp Pte Ltd | ISOLATION OF stem / progenitor cells FOSTER HINDE FROM an umbilical cord |
WO2008129563A2 (en) * | 2007-04-23 | 2008-10-30 | Stempeutics Research Private Limited, | Human mesenchymal stem cells and preparation thereof |
RU2473684C2 (ru) * | 2007-11-27 | 2013-01-27 | Лайфскен, Инк. | Дифференцировка человеческих эмбриональных стволовых клеток |
US9574171B2 (en) * | 2010-12-02 | 2017-02-21 | Technion Research & Development Foundation Ltd. | Methods of generating corneal cells and cell populations comprising same |
CN103184190A (zh) * | 2013-04-11 | 2013-07-03 | 陈云燕 | 一种将脂肪干细胞转化为睾酮分泌细胞的诱导剂及培养基 |
JP2015019628A (ja) * | 2013-07-19 | 2015-02-02 | 学校法人慶應義塾 | 単層細胞層の製造方法 |
KR101690872B1 (ko) * | 2014-08-19 | 2016-12-29 | 이화여자대학교 산학협력단 | 편도 유래 중간엽 줄기세포로부터 인슐린 분비 세포의 분화 방법 |
CN107208052A (zh) * | 2015-01-15 | 2017-09-26 | 国立大学法人大阪大学 | 由多能干细胞向角膜上皮细胞的分化诱导 |
CN104845932A (zh) * | 2015-04-29 | 2015-08-19 | 天津中医药大学 | 淫羊藿苷的新用途 |
CN105132369B (zh) * | 2015-08-25 | 2018-06-26 | 青岛瑞思科生物科技有限公司 | 一种将间充质干细胞转化为睾酮分泌细胞的诱导剂及诱导培养基 |
CN105085938B (zh) * | 2015-08-28 | 2018-03-27 | 广州赛莱拉干细胞科技股份有限公司 | 白芨多糖水凝胶、培养基质及其应用与诱导脐带间充质干细胞向角膜上皮细胞分化的方法 |
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2020
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JPWO2010134619A1 (ja) * | 2009-05-18 | 2012-11-12 | 国立大学法人東北大学 | 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法 |
CN105062970A (zh) * | 2015-08-25 | 2015-11-18 | 北京瑞思德生物医学研究院 | 一种将间充质干细胞诱导成神经细胞的诱导剂及诱导分化完全培养基 |
CN105087466A (zh) * | 2015-08-28 | 2015-11-25 | 广州赛莱拉干细胞科技股份有限公司 | 诱导脐带间充质干细胞向角膜上皮细胞分化的培养基和方法 |
CN105176912A (zh) * | 2015-08-28 | 2015-12-23 | 广州赛莱拉干细胞科技股份有限公司 | 一种培养基及其应用、制备方法 |
Non-Patent Citations (3)
Title |
---|
Differentiation Potential of Limbal Fibroblasts and Bone Marrow Mesenchymal Stem Cells to Corneal Epithelial Cells;Katikireddy, KR等;《STEM CELLS》;20140331;第32卷(第3期);全文 * |
人角膜上皮细胞体外培养的研究进展;王宇静等;《眼科新进展》;20170405;第37卷(第4期);全文 * |
无血清培养的角膜缘干细胞的生物学特性;黄小勇等;《第三军医大学学报》;20070630;第29卷(第11期);全文 * |
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JP7239686B2 (ja) | 2023-03-14 |
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WO2021212592A1 (zh) | 2021-10-28 |
CN111394311A (zh) | 2020-07-10 |
US20220041982A1 (en) | 2022-02-10 |
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JP2022534332A (ja) | 2022-07-29 |
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