CN111394311A - 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 - Google Patents
一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 Download PDFInfo
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Abstract
Description
组别 | 诱导条件 |
本发明诱导组 | 本发明的诱导培养基,诱导5d。 |
条件培养基组 | 条件培养基制备:人角膜缘干细胞用含20%FBS+10μg/LEGF的DMEM/F12培养基,置37°C的5%二氧化碳培养箱,每2d更换培养基一次。收集每次更换的培养基,移入离心管,1000rpm/min离心5分钟,收集上清于无菌管中,置于-20°C冰箱备用。用上述条件培养基诱导30d。 |
共培养组 | 按Millipore公司说明, 用Transwell共培养体系。将人角膜缘干细胞接种到Transwell小室,再放入到6孔板,与间充质干细胞共同培养诱导7d。 |
Claims (2)
Priority Applications (6)
Application Number | Priority Date | Filing Date | Title |
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CN202010310198.9A CN111394311B (zh) | 2020-04-20 | 2020-04-20 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
EP20873344.4A EP4141109A1 (en) | 2020-04-20 | 2020-05-25 | Serum-free complete culture medium capable of inducing differentiation of mesenchymal stem cells from corneal epithelial cells |
US17/283,491 US20220041982A1 (en) | 2020-04-20 | 2020-05-25 | A serum-free complete medium for inducing differentiation of a mesenchymal stem cell to a corneal epithelial cell |
PCT/CN2020/092071 WO2021212592A1 (zh) | 2020-04-20 | 2020-05-25 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
KR1020217008536A KR102552191B1 (ko) | 2020-04-20 | 2020-05-25 | 중간엽 줄기세포가 각막 상피세포로의 분화를 유도하는 무혈청 완전 배지 |
JP2021516585A JP7239686B2 (ja) | 2020-04-20 | 2020-05-25 | 角膜上皮細胞への間葉系幹細胞の分化を誘導する無血清完全培地 |
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CN202010310198.9A CN111394311B (zh) | 2020-04-20 | 2020-04-20 | 一种诱导间充质干细胞向角膜上皮细胞分化的无血清完全培养基 |
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CN111394311A true CN111394311A (zh) | 2020-07-10 |
CN111394311B CN111394311B (zh) | 2022-07-01 |
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Country Status (6)
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US (1) | US20220041982A1 (zh) |
EP (1) | EP4141109A1 (zh) |
JP (1) | JP7239686B2 (zh) |
KR (1) | KR102552191B1 (zh) |
CN (1) | CN111394311B (zh) |
WO (1) | WO2021212592A1 (zh) |
Citations (5)
Publication number | Priority date | Publication date | Assignee | Title |
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JPWO2010134619A1 (ja) * | 2009-05-18 | 2012-11-12 | 国立大学法人東北大学 | 人工多能性幹細胞からの上皮系前駆細胞・幹細胞群及び角膜上皮細胞群の分化誘導方法 |
US20130251692A1 (en) * | 2010-12-02 | 2013-09-26 | Institut National De La Sante Et De La Recherche Medicale | Methods of generating corneal cells and cell populations comprising same |
CN105062970A (zh) * | 2015-08-25 | 2015-11-18 | 北京瑞思德生物医学研究院 | 一种将间充质干细胞诱导成神经细胞的诱导剂及诱导分化完全培养基 |
CN105087466A (zh) * | 2015-08-28 | 2015-11-25 | 广州赛莱拉干细胞科技股份有限公司 | 诱导脐带间充质干细胞向角膜上皮细胞分化的培养基和方法 |
CN105176912A (zh) * | 2015-08-28 | 2015-12-23 | 广州赛莱拉干细胞科技股份有限公司 | 一种培养基及其应用、制备方法 |
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JP7239686B2 (ja) | 2023-03-14 |
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