CN106282052B - It is a kind of improve coastal saline soil compound lactobacillus preparation and its application - Google Patents
It is a kind of improve coastal saline soil compound lactobacillus preparation and its application Download PDFInfo
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Abstract
The invention discloses a kind of compound lactobacillus microbial inoculums for improveing coastal saline soil and its application, the microbial inoculum to be made of lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang.Both bacterium have apparent influence to the growth metabolism of plant on saline-alkali soil, can cooperate with germination percentage, uniformity, the uniformity and the plant strain growth ability for improving plant.
Description
Technical field
The present invention relates to a kind of microbial bacterial agents more particularly to one kind can improve coastal saline soil, significantly improves plant
The compound lactobacillus preparation of object salt resistance alkaline stress, belongs to microorganisms technical field.
Background technique
The Huanghe delta is China or even the fastest Coastal Ecosystem of world's epeirogenetic, new since 1855 change its course
Increase more than 6000 square kilometres of land area, newborn soil provides wide standby cultivated land resource, is ensureing China's land resource
There is considerable effect in safety and ecosystem service function.But in recent years, the blindness cultivation of the mankind utilizes, cause
Main Agricultural utilizes soil in area --- and the Saline of new alluvial plain and improvement, just the accumulation of salt in the surface soil is degenerated at an amazing speed, in addition gas
Waiting variation influences, and self-purification capacity, resistivity and the recovery capability of ecological environment are deteriorated, and there are about 5% new alluvial plains to become every year
Heavy saline and saline-alkali wasteland, ecosystem function are degenerated seriously, and land and water resources environmental quality decline, recovery has become with reconstruction
For current research hotspot.
Being blended shallow, salty underground water by Seawater immersion side is influenced, beach saline land have salinity is high, easy to harden, structure is poor,
The characteristics such as the content of organic matter is low, storing water and preserving fertility ability is weak determine its agriculture during the initial stage of enclosing tideland for cultivation to desalination to normal soil
Industry is very big using difficulty, while improperly agricultural use mode makes the salinization of soil have repeatability, how efficient explores
It is a problem to be solved coastal saline-alkali land reform is good for planting technique soil.
Currently, coastal saline-alkali soil improving technology mainly uses such as: spraying water discharge method, by soil moved in improve the original improvement, improving straw mulching
Equal physical methods or chemical method using some acid salts substances improvement, but these methods there are engineering amounts big, consumption
Water conservancy projects that its needs of the defects of money is high, the period is long, such as " irrigation and drainage by well " method and " forced-ventilated fills by force " method are established invest it is huge,
Maintenance cost is high；Soil moved in improve the original improvement not only needs to transport a large amount of soil moved in improve the original, and often to soil moved in improve the original source place farmland cause it is huge
Big destruction；For chemical method due to will constantly suffer erosion directly facing sea and before and after improvement, effect is very limited,
It is difficult to promote.
Biological modification technology is compared with above-mentioned measure, with low in cost, material source is extensive, easy to use, promotion is made
Object root system, accelerate crumb structure to be formed, process is green safe, the environmental-friendly advantages such as without side-effects.However, current biology changes
Good technology is only for the improvement in the salt-soda soils such as northwest, the Northeast, with having no suitable Yellow River Delta Coastal beach saline-alkali
The relevant technologies measure.
Summary of the invention
For the difficult problem of Huanghe delta standby cultivated land resource development and utilization, the object of the present invention is to provide one kind
Improve composite bacteria agent and its application of coastal saline soil.
In order to achieve the object of the present invention, inventor is finally obtained as follows by a large number of experiments research and unremitting effort
Technical solution:
A kind of compound lactobacillus microbial inoculum for improveing coastal saline soil, the microbial inoculum is by lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang composition.
It should be noted that lactobacillus plantarum (Lactobacillus plantarum) P-8 of the present invention, point
From from Inner Mongol traditional natural fermentation yoghurt, there is excellent anti-gastrointestinal tract digestive juice tolerance, can survive in dynamic
In object enteron aisle；The bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, preservation time are on 06 28th, 2012, and deposit number is CGMCC No.6312.
In addition, Lactobacillus casei (Lactobacillus casei) Zhang of the present invention, be separated from koumiss it is resistance to
The probiotics strain of sour and resistance to bile acid；The bacterial strain is preserved in Chinese microorganism strain preservation management committee on April 21st, 2006
Member's meeting General Microbiological Culture collection, deposit number CGMCC No.1697.
Preferably, composite bacteria agent as described above, the microbial inoculum is by lactobacillus plantarum (Lactobacillus plantarum
P-8) and Lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5~3) number of viable than composition.
Composite bacteria agent of the present invention can be prepared with the following method:
(1) by the lactobacillus casei bacterial strain (Lactobacillus casei) Zhang and lactobacillus plantarum strain
(Lactobacillus plantarum) P-8 is inoculated in MRS culture solution, cultivates 12-24 hours under the conditions of 37 DEG C, through being centrifuged
After washing, 8%-12% sterilized non-fat cow's milk liquid is added on bacterial sediment, and adjusting its bacterium number is 2.0 × 1010Cfu/mL is mixed
Culture is poured into after even；
(2) strain culture that step (1) obtains is inoculated in MRS culture solution, it is small that 12-24 is cultivated under the conditions of 37 DEG C
When, inoculate the sterilized non-fat cow's milk liquid of 8%-12%, cultivated 6-10 hours under the conditions of 37 DEG C, adjust its bacterium number be 3 ×
109Cfu/mL, pH value are 4.4~4.6.
The present inventor has been surprisingly found that when screening saline-alkali tolerant bacterial strain, contains lactobacillus plantarum (Lactobacillus
Plantarum) composite bacteria liquid of P-8 and Lactobacillus casei (Lactobacillus casei) Zhang have certain saline-alkali tolerant to make
With, and have apparent facilitation to the growth metabolism of plant on saline-alkali soil.Based on this discovery, the present inventor is by being inoculated in
Both bacterium have been prepared into composite bacteria agent by cultivation and fermentation in MRS culture solution.Therefore, the present invention provides above-mentioned composite bacteria agents
Application, it may be assumed that
A kind of composite bacteria agent is improving the application in plant anti-salt stress, and the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) number of viable is than composition.
A kind of composite bacteria agent is improving the application in Genes For Plant Tolerance alkaline stress, and the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) number of viable is than composition.
A kind of composite bacteria agent improves the application in plant anti-salt alkali resistant stress at the same time, and the composite bacteria agent is by plant cream
Bacillus (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 1:
The number of viable of (0.5~3) is than composition.
A kind of application of composite bacteria agent in improvement coastal saline soil, the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) number of viable is than composition.
A kind of application of composite bacteria agent in preparation reclamation of salinep-alkali soil liquid, the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) number of viable is than composition.
It should be strongly noted that above-mentioned composite bacteria agent sprays after being prepared into bacterium solution in the embodiment that the present invention records
Peanut or Salt-resistant of Cotton alkali resistant stress can be improved, and promote its good germination, growth.
In addition, the present invention also provides a kind of implantation methods of raising peanut salt resistance alkali resistant stress, this method comprises: broadcasting
Kind preceding plot is ploughed deeply, in conjunction with plough 200~300 jin of urea of per hectare application, 300~400 jin of Diammonium phosphate (DAP), potassium sulfate 100~
150 jin of ridging suppressions, to sow 10000~11000 caves per acre, every cave 1-3 plants, after planting cover mulch, the later period no longer carries out
Top dressing and irrigation；After peanut seeding terminates, spraying total viable count is 1.0 × 109~6.0 × 109The infected water of cfu/mL,
The amount of spraying is 2-3 liter/mu；Spraying total viable count again in the peanut gynophore phase is 1.0 × 109~6.0 × 109Cfu/mL's contains
Bacterium water, the amount of spraying are 2-3 liter/mu.
Compared with prior art, composite bacteria agent of the present invention is by lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang composition, both bacterium on saline-alkali soil to planting
The growth metabolism of object has apparent influence, can cooperate with germination percentage, uniformity, the uniformity and the plant strain growth energy for improving plant
Power.
Specific embodiment
Form is described in further detail above content of the invention again by the following examples, but should not manage this
For solution for the scope of the above subject matter of the present invention is limited to the following embodiments, all technologies realized based on above content of the present invention are equal
Belong to the scope of the present invention.In addition, the experimental method in following embodiments is unless otherwise instructed conventional method.
Embodiment 1: lactobacillus plantarum (Lactobacillus plantarum P-8) and Lactobacillus casei
(Lactobacillus casei) Zhang is in the test for improving salt-soda soil fresh branches and leaves of peanut seedling growing way, uniformity and the uniformity
Test place: the Dongying City Lijin County town the Ting Luo village Mao Tuo-moderate saline-alkali soil (lactobacillus plantarum group, Lactobacillus casei
Group, compound bacteria group and each 1 mu of blank control group)
Cultivate administration: sowing preceding plot and plough deeply, and applies 200~300 jin of urea, Diammonium phosphate (DAP) by per hectare in conjunction with ploughing
300~400 jin, the suppression of 100~150 jin of potassium sulfate of standard ridging, to sow 10000~11000 caves per acre, 2 plants of every cave is broadcast
Mulch is covered after kind.Later period no longer carries out top dressing and irrigation.
Experimental period: 2016.06.2-2016.8.4
Test crop: peanut
Usage amount and method: after peanut seeding terminates, experimental group sprays the microbial bacterial agent (bacterium that experimental group uses immediately
Total viable count content 3.0 × 10 in agent9Cfu/mL, the quantity ratio of two kinds of bacterium is 1:1 in compound bacteria group) 2 liters/mu.In peanut
The needle phase sprays microbial bacterial agent again, and (total viable count content is greater than 10 in the microbial inoculum that experimental group uses9Cfu/mL, compound bacteria group
In the quantity ratios of two kinds of bacterium be 1:1) 3 liters/mu, control group is identical as other way to manages of experimental group.
After peanut seeding 15 days, each sample prescription for handling plot and randomly selecting 52 ㎡ respectively measures germination percentage；The gynophore phase
After fertilising 15 days, each sample prescription for handling plot and randomly selecting 52 ㎡ respectively measures peanut plant height.
1 each group field experiment peanut germination percentage of table and plant height compare
Compared with blank control group,▲P < 0.01；Compared with lactobacillus plantarum group,★P < 0.01；With Lactobacillus casei group
Compare,■P < 0.01.
By the test statistics result of table 1 it is found that using compound lactobacillus preparation on saline-alkali soil, peanut seed can be improved
The plant height of germination percentage and plant has extremely significant difference (p < 0.01), relatively compared with blank control group or single bacterium group
In blank control group, Lactobacillus casei group, lactobacillus plantarum group, the germination percentage of peanut has been respectively increased 24.99%, 23.08%,
19.09%, plant height has been respectively increased 20.78%, 16.22%, 25.0%.
Embodiment 2: lactobacillus plantarum (Lactobacillus plantarum P-8) and Lactobacillus casei
Influence of (Lactobacillus casei) Zhang to cotton germination rate, seedling length and root long under Salt Strees Condition is tested
Test place: Qingdao Agricultural University's experimental teaching of agronomy room
Test setting: we early-stage study have found that yellow delta coastal saline-alkali area topsoil base cation is main are as follows:
Na+、HCO3-、Cl-、SO3 2-, in order to simulate the strand nature solonchak ingredient, we use sodium chloride, sodium sulphate and sodium bicarbonate
Three kinds of mixed salts, equal proportion (1:1:1) are mixed into the foundation soil after natural air drying, sieving impurity elimination, are classified according to soil salinity characters
It is as follows to configure 4 salinity gradients for standard: conventional (0.6g/kg), light salt (1.1g/kg), middle salt (2.9g/kg), geavy salt (5.1g/
kg)。
The soil of configured different salinity gradients is laid in the culture dish of diameter 9cm, it is added in each culture dish
Soil amount is identical, and each culture dish sows 20 seeds, and seed sowing is uniform as far as possible, make each seedling have sufficient growing space and
Nutriment.
Experimental period: 2016.7.6-2016.7.13
Test crop: cotton
Usage amount and method: each salinity gradient divides into lactobacillus plantarum group, Lactobacillus casei group, compound bacteria group and blank
Control group, by bacterium solution (total viable count content 5.0 × 109Cfu/mL, the quantity ratio of two kinds of bacterium is 1:1 in compound bacteria group) with 1:
After 500 concentration dilution, each culture dish adds 1ml dilution, and blank control adds the distilled water of equivalent, uses and claim daily later
Weight method supplements sterile distilled water.
All culture dishes are put into 25+1 DEG C of biochemical cultivation case and are germinateed.Seed is counted in the 7d of Seed germination
Germinative number calculates germination percentage (germination percentage (%)=broadcast rear 7d chitting piece number/sowing seed number * 100%).Germination standard is embryo
Root reaches the 1/3 of seed length.The 7d of Seed germination terminates to germinate, and measures the long (height of seedling: hypocotyl base portion to bud top of seedling of seedling
The length at end), main root length (main root is long: the length of hypocotyl base portion to main root tip).
Cotton germination rate, height of seedling and root long under each salinity gradient of 2 embodiment of table 2
Compared with blank control group,▲P < 0.01；Compared with lactobacillus plantarum group,★P < 0.01；With Lactobacillus casei group
Compare,■P < 0.01.
By 2 test result of table it is found that under non-saline-alkali environment, either compound bacteria group or single bacterium group compare blank pair
The germination percentage for improving cotton can be limited according to group and seedling is long (P < 0.01), and the seedling for significantly improving cotton is long.And compound bacteria group with
Lactobacillus plantarum group, Lactobacillus casei group single bacterium group compare no significant difference (P > 0.05), imply in nonsaline-alkali soil ground,
Lactobacillus plantarum or Lactobacillus casei can limit the germination and growth for promoting cotton.
In addition, using compound lactobacillus preparation on saline-alkali soil, it can be improved the germination percentage of cotton seeds, different Salt Strees Conditions
Under be all remarkably higher than blank control group, lactobacillus plantarum group or Lactobacillus casei group (P < 0.01), this imply that lactobacillus plantarum
The germination percentage for improving cotton can be cooperateed with by being used in combination with Lactobacillus casei.And with the raising of soil salts density, compound bacteria
Group enhances the growth-promoting ability of Levant Cotton Root, it is light, in, under severe salinity, compound bacteria group is respectively increased relative to blank control group
12.03%, 16.73% and 155.86%.
Compound lactobacillus preparation is used on saline-alkali soil, the seedling that can be improved cotton seeds is long, significant under slight Salt Strees Condition
Higher than blank control group, lactobacillus plantarum group or Lactobacillus casei group (P < 0.01), this imply that lactobacillus plantarum and cheese cream
Bacillus is used in combination can cooperate with the seedling for improving cotton long under low saline-alkali environment.Relative to control energy on slight and heavy salinized soil
The seedling for enough significantly improving cotton seeds is long, though it is long to improve seedling on moderate solonchak, the no significant difference (P between blank control group
> 0.05).Gently, in, under severe salinity, 42.31%, 10.21% and is respectively increased relative to blank control group seedling length
52.51%.
Compound lactobacillus preparation is used on saline-alkali soil, can be increased the root long of cotton seeds, be shown under different Salt Strees Conditions
It writes and is higher than blank control group, lactobacillus plantarum group or Lactobacillus casei group (P < 0.01), this imply that lactobacillus plantarum and cheese
Lactobacillus, which is used in combination, can cooperate with the root long for improving cotton.And with the raising of soil salts density, compound bacteria group is to Levant Cotton Root
Growth-promoting ability it is most strong, it is light, in, under severe salinity, 21.89%, 22.38% and is respectively increased relative to blank control group
30.80%.
Claims (4)
1. a kind of composite bacteria agent is improving the application in plant anti-salt stress, the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) than forming, the deposit number of the lactobacillus plantarum (Lactobacillus plantarum) P-8 is number of viable
The deposit number of CGMCC No.6312, the Lactobacillus casei (Lactobacillus casei) Zhang are CGMCC
No.1697, the plant are peanut or cotton.
2. a kind of composite bacteria agent is improving the application in Genes For Plant Tolerance alkaline stress, the composite bacteria agent is by lactobacillus plantarum
(Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang by 1:(0.5~
3) than forming, the deposit number of the lactobacillus plantarum (Lactobacillus plantarum) P-8 is number of viable
The deposit number of CGMCC No.6312, the Lactobacillus casei (Lactobacillus casei) Zhang are CGMCC
No.1697, the plant are peanut or cotton.
3. a kind of composite bacteria agent improves the application in plant anti-salt alkali resistant stress at the same time, the composite bacteria agent is by plant cream bar
Bacterium (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang presses 1:(0.5
~3) than forming, the deposit number of the lactobacillus plantarum (Lactobacillus plantarum) P-8 is number of viable
The deposit number of CGMCC No.6312, the Lactobacillus casei (Lactobacillus casei) Zhang are CGMCC
No.1697, the plant are peanut or cotton.
4. a kind of implantation methods for improving peanut salt resistance alkali resistant stress, which is characterized in that this method comprises: plot is deep prior to seeding
It turns over, in conjunction with per hectare application 200~300 jin of the urea, 100~150 jin of 300~400 jin of Diammonium phosphate (DAP), potassium sulfate ridging towns of ploughing
Pressure, to sow 10000~11000 caves per acre, every cave 1-3 plants, after planting covers mulch, the later period no longer carries out top dressing and irrigation；
After peanut seeding terminates, spraying total viable count is 1.0 × 109~6.0 × 109 The infected water of cfu/mL, the amount of spraying are 2-3
Liter/mu；Spraying total viable count again in the peanut gynophore phase is 1.0 × 109~6.0 × 109 The infected water of cfu/mL, the amount of spraying
For 2-3 liter/mu；Bacterium in the infected water is by lactobacillus plantarum (Lactobacillus plantarum) P-8 and cheese cream
Bacillus (Lactobacillus casei) Zhang press 1:(0.5~3) number of viable than composition, the lactobacillus plantarum
The deposit number of (Lactobacillus plantarum) P-8 is CGMCC No.6312, the Lactobacillus casei
The deposit number of (Lactobacillus casei) Zhang is CGMCC No.1697.
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| CN107129952B (en) * | 2017-06-19 | 2020-05-05 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | Lactobacillus composite microbial agent for improving yield and characters of peanuts in saline-alkali soil and application thereof |
| CN107129951B (en) * | 2017-06-19 | 2020-07-07 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof |
| CN108410470B (en) * | 2018-03-05 | 2020-10-09 | 中国农业科学院农业环境与可持续发展研究所 | Microbial soil conditioner for saline-alkali soil and preparation method thereof |
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