CN109127722A - It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure - Google Patents
It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure Download PDFInfo
- Publication number
- CN109127722A CN109127722A CN201811101424.1A CN201811101424A CN109127722A CN 109127722 A CN109127722 A CN 109127722A CN 201811101424 A CN201811101424 A CN 201811101424A CN 109127722 A CN109127722 A CN 109127722A
- Authority
- CN
- China
- Prior art keywords
- plant
- heavy metal
- mycorrhizal
- metal pollution
- summer cypress
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
- B09C1/10—Reclamation of contaminated soil microbiologically, biologically or by using enzymes
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C1/00—Reclamation of contaminated soil
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B09—DISPOSAL OF SOLID WASTE; RECLAMATION OF CONTAMINATED SOIL
- B09C—RECLAMATION OF CONTAMINATED SOIL
- B09C2101/00—In situ
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Soil Sciences (AREA)
- Environmental & Geological Engineering (AREA)
- Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Mycology (AREA)
- Cultivation Of Plants (AREA)
Abstract
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized the present invention provides a kind of.Technical method of the invention is the indigenous microorganism for utilizing tailing area, and after screening separation, it is numerous to carry out culture expansion to dominant microflora volume branch Mucor；Choose that black nightshade group, alfalfa group, root system is luxuriant in Kochia simultaneously, Saline alkali tolerance has the plant of enrichment as target plant in heavy metal, it is infected with rolling up branch Mucor progress root, Mycorrhizal plant is formed, enhancing plant root activity improves enrichment to heavy metal, degradation；Compared with existing phytoremediation technology, this method has remediation efficiency high, easy to operate, and environmental suitability is strong, can large-area applications in the iron tailings pollution amelioration the characteristics of.
Description
Technical field
The invention belongs to heavy metal pollution recovery technique in situ fields, and in particular to a kind of heavy in situ using Mycorrhizal plant
Metallic pollution restorative procedure.
Background technique
China is the country of a rich in mineral resources, and Resource security is also more complete, resulting mine wastes product
Storage and annual emissions are very huge.Currently, China whole nation year Ore Yield sum is more than more than 50 hundred million tons, tailing waste production is occupied
It is high not under, tailing discharge amount in 2000 oneself through up to more than 600,000,000 tons, 2008, which are only the tailing waste directly produced by mine, just 6.7 hundred million
Ton.Heavy metal element is rich in iron tailings, extensive prolonged iron tailings is stacked, not only expends management operating cost, occupy
Land resource, and cause part mining area periphery and downstream Soil Environmental Pollution and ecological disruption, and then threaten human health.I
State is large agricultural country, and heavy metal pollution of soil will can seriously affect China's food safety, ecological safety and Environmental security.
Heavy metal has the characteristics that be difficult to degrade, is easy accumulation, is more toxic, currently used for administering the soil of heavy metal pollution
Earth mainly uses high enriching plant, but the method biomass is small, root system is shallow, distinctive environmental and relatively narrow annidation etc.
Feature is restricted phytoremediation in many cases.
System of the mycorrhizal plants as plant in soil and its rhizosphere microorganism symbiosis can expand root system absorbing surface, increase
Add the absorbability to the nutrient outside primitive root hair absorption region, promotes plant growth.Meanwhile plant underground part epidermal cell
Shedding, nutriment and enzyme r e lease also provide more favorable life condition for the microorganism in soil system, in educating for plant
Seedling, enhancing stress resistance of plant etc. have a wide range of applications.
The present inventor has finally found a kind of utilize in a large number of experiments and rolls up a branch Mucor progress Mycorrhizal plant huge sum of money in situ
Belong to pollution amelioration method.
Summary of the invention
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized the object of the present invention is to provide a kind of.
The purpose of the present invention is what is realized by following technical proposals:
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized according to of the present invention, which is characterized in that including
Following steps:
(1) prepared by bacterium solution:
1. using activation medium inoculation volume branch Mucor, in 28~30 DEG C, 180~220rmin-1Shaking table on cultivate 1
It~3 days, is activated；
2. by the volume branch Mucor after activation using expansion breeding culture medium in 28~30 DEG C, 180~220rmin-1Shaking table on
Oscillation expand numerous for 20~28 hours；
3. being diluted to 1~9 × 10 for numerous volume branch Mucor is expanded with sterile water again7The bacterium solution of CFU/ml to get；
The wherein inoculation and expansion breeding culture medium ingredient are as follows: peptone: 3.0~5.0g/L, yeast extract: 1.0~
2.0g/L, 10~20.0g/L of glucose, 1.0~2.0g/L of dipotassium hydrogen phosphate, magnesium sulfate 0.5~2.5g/L, pH=6.4 ±
0.2；The expansion breeding culture medium ingredient are as follows: 3.0~6.0g/L of potato leaching powder, 10~20.0g/L of glucose, agar 10~
20.0g/L, pH=5.6 ± 0.2.
(2) root is infected:
Select black nightshade group, alfalfa group, summer cypress platymiscium as target plant, mode of infection are as follows:
To vegetable seeds vernalization, after germination, transplant in the soil of heavy metal pollution, at the same drench at transplanting into
Mucor circinelloides liquid makes microbial infection plant root, forms Mycorrhizal plant；
Or vegetable seeds is impregnated into diel, it is seeded in the soil of heavy metal pollution, while drenching at sowing into volume branch
Mucor bacterium solution；Make microbial infection root after plant seed germination growth, forms Mycorrhizal plant；
Wherein Mucor circinelloides in the soil at every chitting piece transplanting or at every seed sowing
Liquid additional amount is 10~50mL/m2。
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized according to of the present invention, which is characterized in that described
The thickness of sowing of chitting piece or seed is 10~40 plants/m2。
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized according to of the present invention, which is characterized in that described
Black nightshade group plant includes: red fruit black nightshade, black nightshade, S. photeinocarpum, the wooden black nightshade.
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized according to of the present invention, which is characterized in that described
Alfalfa group plant includes: wild alfalfa, and alfalfa hybridizes clover.
Mycorrhizal plant original position heavy metal pollution restorative procedure is utilized according to of the present invention, which is characterized in that described
Summer cypress platymiscium includes: Iranian summer cypress, full wing summer cypress, hair flower summer cypress, black wing summer cypress, sharp wing summer cypress, prostrata summercypress, summer cypress.
The black nightshade group plant includes: red fruit black nightshade Solanum alatum Moench, black nightshade Solanum nigrum
L, S. photeinocarpum Solanum photeinocarpum Nakam.&0dash, the wooden black nightshade Solanum suffruticosum
Schousb；
The alfalfa group plant includes: wild alfalfa Medicago falcata L, alfalfa Medicago sativa
L hybridizes clover Medicago varia Martyn；
The summer cypress platymiscium includes Iranian summer cypress Kochia iranica Litv.ex Bornm., full wing summer cypress
Kochia krylovii Litv., hair flower summer cypress Kochia laniflora (S.G.Gmel.) Borb.Borb., black wing summer cypress
Kochia melanoptera Bunge, sharp wing summer cypress Kochia odontoptera Schrenk, prostrata summercypress Kochia
Prostrata (L.) Schrad., summer cypress Kochia scoparia (L.) Schrad..
Strain of the invention rolls up branch Mucor (Mucor circinelloides), for Dutch Culture Collection
(CBS), bacterium numbering CBS131818.The bacterial screening has heavy metal tolerance from the soil containing heavy metal, and through breeding
The ability of environment；Using the indigenous microorganism in iron tailings area, after screening separation, culture expansion is carried out to dominant bacteria volume branch Mucor
It is numerous；Choose that black nightshade group, alfalfa group, root system is luxuriant in Kochia simultaneously, Saline alkali tolerance has the plant of enrichment to heavy metal
Object is infected as target plant with rolling up branch Mucor progress root, forms Mycorrhizal plant, enhancing plant root activity, raising pair
The enrichment of heavy metal, degradation；Compared with existing phytoremediation technology, this method is high, easy to operate with remediation efficiency,
Environmental suitability is strong, can large-area applications in the iron tailings pollution amelioration the characteristics of.
The present invention utilizes the distinctive syntaxial system of Mycorrhizal plant, and volume branch Mucor, can not only in the attachment of plant root
Expand root system of plant absorbing surface, increase the absorbability to the nutrient outside primitive root hair absorption region, promotes plant growth, and
And plant is improved to the accumulation ability of heavy metal, also, the volume branch Mucor of this syntaxial system can after in conjunction with root system of plant
To secrete special secretion, change root system of plant surrounding soil physicochemical property, rhizosphere environment and by the way that oxygen occurs
Change reduction reaction to change the existing forms of heavy metal, reduce its toxicity, further promotes plant to the absorption of heavy metal,
Improve removal efficiency.
Heavy metal removing rate of the present invention is preferable, especially higher to the removal rate of Cd, Pb.The Mycorrhizal chosen in the present invention
Plant growth medium requirement is low, can grow in this slight alkali environment of tailing.The present invention can improve plant growth slowly,
The problems such as biomass is small, repairing efficiency is long.As a kind of environmentally friendly and economical and effective type green technology, have great
Society, economy and ecological management benefit, while also having input cost lower, environment influences lesser advantage.
Specific embodiment
Below with reference to embodiment, present invention is further described in detail:
Roll up the choosing method of branch Mucor are as follows: carry out to the tailing area microorganism of level in Anshan city, liaoning province Qianshan District Dagushan Iron Ore Mine
Screening separation；Microorganism is tamed, heavy metallic salt solution is added in the medium, steps up concentration, selective advantage bacterium
Kind, the dominant bacteria is identified to be confirmed as existing strain-volume branch Mucor (Mucor circinelloides), Dutch microbial bacteria
Kind collection (CBS), bacterium numbering CBSl31818；Strain is inoculated into fluid nutrient medium, in constant temperature culture oscillator
It is cultivated, cultivation temperature is 28~32 DEG C.Culture medium is in 28 DEG C, 180rmin-1Shaking table on cultivate 2d, to volume branch Mucor
It is activated, the 250ml that the volume branch Mucor after activation is inoculated into high-temperature sterilization processing has been added in the conical flask of culture medium,
In 32 DEG C, 220rmin-1Shaking table on vibrate for 24 hours, bacterium solution is diluted to 1~5 × 10 with sterile water7CFU/mL (blood count
Plate counts).
Inoculation and expansion breeding culture medium ingredient are as follows: peptone 5.0g/L, yeast extract 2.0g/L, glucose 20.0g/L, phosphoric acid
Hydrogen dipotassium 1.0g/L, magnesium sulfate 0.5g/L, pH=6.5；The fungi expands breeding culture medium ingredient are as follows: potato leaching powder 6.0g/
L, glucose 20.0g/L, agar 20.0g/L, pH=5.6.
Embodiment 1
Carry out potting simulated experiment in Shandong University's Environmental Science and Engineering institute heliogreenhouse, verifies Mycorrhizal plant pair
The efficient removal of heavy metal acts on.
Using the common commercially available flowerpot of diameter 20cm, the western Anshan Iron tail in level in Anshan city, liaoning province Qianshan District is derived from for examination soil
Mining area and by volume 1:1 add normal soil, for simulating the physicochemical properties such as tailing meta-alkalescence, retain water and nutrients ability difference；For
Examination soil is mixed, is air-dried, crossing 2 mm sieves, ultraviolet sterilization for 24 hours；
Planting condition of culture are as follows: 22-32 DEG C of day temperature, gravity-flow ventilation；17-22 DEG C of nocturnal temperature, closing；
Heavy metal Cd, Pb are added in examination soil, concentration gradient is arranged are as follows: Cd:0mgkg in the form of salting liquid-1、
10mg·kg-1、30mg·kg-1、50mg·kg-1、100mg·kg-1；Pb:0mgkg-1、50mg·kg-1、100mg·kg-1、
300mg·kg-1、500mg·kg-1；Every group of experiment three parallel, and natural equilibrium 2 weeks is for use after mixing.
By the vegetable seeds of purchase, respectively S. photeinocarpum, black nightshade, the wooden black nightshade；Wild alfalfa, alfalfa, hybridization clover；Hair
Flower summer cypress, prostrata summercypress, summer cypress are placed on the culture dish for being covered with wet filter paper, set after distilled water immersion one day of 37 DEG C or so
The vernalization in incubator (28 DEG C of temperature, humidity 80%), appropriate water spray is daily to keep filter paper wet；After germination, broadcast
Kind is into the flowerpot equipped with processing soil sample, 3, every basin；
Using culture medium in 28~32 DEG C, 180rmin-1Shaking table on cultivate 2d, to volume branch Mucor activate, will live
The 250ml that volume branch Mucor after change is inoculated into the processing of high-temperature sterilization has been added in the conical flask of culture medium, 28~32 DEG C,
220r·min-1Shaking table on vibrate for 24 hours, bacterium solution is diluted to 1~1.5 × 10 with sterile water7CFU/mL (blood counting chamber meter
Number), it is applied separately to the soil of nursery stock root, every basin seedling inoculation bacterium solution amount is 80mL, volume branch Mucor and plant co-cultured,
Microbial infection plant root forms Mycorrhizal plant；
According to soil dry-wet situation, moisturizing of watering daily records the growing state of plant.In entire growing process,
Every 2d changes the direction of basin, measures heavy metal in soil content after 60 days from transplanting certainly and calculates removal rate, removal effect
As shown in the table.
Table 1, Mycorrhizal plant are to the removal rate of Cd in soil
Table 2, Mycorrhizal plant are to the removal rate of Pb in soil
Embodiment 2
Mycorrhizal plant is applied to level in Anshan city, liaoning province Qianshan District Dagushan Iron Ore Mine tailing area.
Tailing soil particle forms based on sand grains, middle sand content 19.2%, fine content 43.5%, and sticky content is only
3.68%, Cd content are 0.09mg/kg, and Pb content is 6.95mg/kg, and the iron tailings content of organic matter is extremely low, and iron tailings is in alkalinity
8.72, iron tailings moisture holding capacity is poor, and for effective potassium content compared with horn of plenty, comprehensive fertility situation is low.
Choose long 5.2m, width 3.5m area is 16m2Experimental field carry out the experiment of Mycorrhizal phytoremediation.
Adding appropriate self-control modifying agent in experimental field, (modifying agent and tailing ratio are 1: 9, and the ingredient of improvement includes biological
Charcoal, organic fertilizer, rice husk and haydite, ratio 2: 1: 1: 1) and turning over, and ploughed depth 30cm, the pH of improved rear tailing are
7.8±0.2。
Vegetable seeds is sowed in experimental field, planting density is about 20 plants/m2, while every plant drenches into 50mL/m2Concentration is 1
~1.5 × 107The Mucor circinelloides liquid of CFU/mL, the microbial infection plant root after germination growth, forms Mycorrhizal
Plant；Then a water is poured every two weeks；After 60 days, plant grows fine, and the content of sampling determination heavy metal in soil measures bacterium
Root S. photeinocarpum, Mycorrhizal black nightshade, Mycorrhizal wood black nightshade are respectively 44%, 47%, 39% to the removal rate of Cd, are gone to Pb
Except rate is respectively 39%, 42%, 35%；Mycorrhizal wild alfalfa, Mycorrhizal alfalfa, Mycorrhizal hybridize clover to the removal rate of Cd
Respectively 31%, 29%, 34%, the removal rate to Pb are respectively 27%, 25%, 32%；Mycorrhizal summer cypress, Mycorrhizal wood ground
Skin, Mycorrhizal hair flower summer cypress are respectively 35%, 33%, 33% to the removal rate of Cd, the removal rate to Pb is respectively 41%,
40%, 38%；
It according to the implementation steps of embodiment 2, and is only drenched in experimental field soil into equal amount bacterium solution, planting plants, not right
The removal rate of Cd and Pb is respectively 24% and 21%.
Comparative example 1
Another sowing mountain lactuca sativa seeds are planted and are watered according to the method for embodiment 2, and are not inoculated with bacterium solution；
After 60 days, plant is short and small, withered and yellow, the content of sampling determination heavy metal in soil, and the removal rate for measuring Cd is 7%,
The removal rate of Pb is 12%.
Comparative example 2
Another sowing mountain lactuca sativa seeds, are planted and watering according to the method for embodiment 2, according to the inoculum concentration of embodiment 2 drench into
Bacterium solution；
After 60 days, plant is short and small, withered and yellow, the content of sampling determination heavy metal in soil, and the removal rate for measuring Cd is 15%,
The removal rate of Pb is 18%.
Comparative example 3
S. photeinocarpum, alfalfa and hair flower summer cypress seed are taken, plants and waters according to the method for embodiment 2, and do not connect
Kind bacterium solution；
After 60 days, plant grows fine, the content of sampling determination heavy metal in soil, measures removal of the S. photeinocarpum to Cd
The removal rate that rate is 25%, Pb is 21%；Alfalfa is 18% to the removal rate that the removal rate of Cd is 17%, Pb；Hair flower summer cypress
The removal rate that removal rate to Cd is 17%, Pb is 23%.
Embodiment 3
Using method described in embodiment 2, difference is to drench at the soil of every vegetable seeds sowing into 20mL concentration
It is 1.33~2 × 107The Mucor circinelloides liquid of CFU/mL；Planting density is 40 plants/m2。
After 60 days, plant grows fine, and the content of sampling determination heavy metal in soil measures Mycorrhizal S. photeinocarpum, bacterium
Root black nightshade, Mycorrhizal wood black nightshade be respectively 29% to the removal rate of Cd, 33%, the removal rate of 28%, Pb be respectively 34%,
38%, 33%；Mycorrhizal wild alfalfa, Mycorrhizal alfalfa, Mycorrhizal hybridization clover be respectively 18% to the removal rate of Cd,
18%, the removal rate of 22%, Pb is respectively 21%, 20%, 24%；Mycorrhizal summer cypress, Mycorrhizal prostrata summercypress, Mycorrhizal hair flower ground
Skin is respectively 28% to the removal rate of Cd, 23%, the removal rate of 25%, Pb be respectively 34%, 28%, 31%；
It according to the implementation steps of embodiment 3, and is only drenched in experimental field soil into equal amount bacterium solution, planting plants, not right
The removal rate of Cd and Pb is respectively 13% and 15%.
Embodiment 4
Using method described in embodiment 2, difference be at the soil of every vegetable seeds sowing to drench in bacterium solution into
80mL concentration is 2.5~3 × 107The Mucor circinelloides liquid of CFU/mL；Planting density is 10 plants/m2。
After 60 days, plant grows fine, and the content of sampling determination heavy metal in soil measures Mycorrhizal S. photeinocarpum, bacterium
Root black nightshade, Mycorrhizal wood black nightshade be 49% to the removal rate of Cd, 54%, the removal rate of 48%, Pb be 51%, 56%, 49%；
Mycorrhizal wild alfalfa, Mycorrhizal alfalfa, Mycorrhizal hybridization clover are the removal of 38%, 39%, 43%, Pb to the removal rate of Cd
Rate is 37%, 35%, 41%；It is 48% to the removal rate of Cd that Mycorrhizal summer cypress, Mycorrhizal prostrata summercypress, Mycorrhizal hair, which spend summer cypress,
43%, the removal rate of 44%, Pb is 52%, 46%, 48%；
It according to the implementation steps of embodiment 4, and is only drenched in experimental field soil into equal amount bacterium solution, planting plants, not right
The removal rate of Cd and Pb is respectively 28% and 19%.
Claims (5)
1. a kind of utilize Mycorrhizal plant original position heavy metal pollution restorative procedure, which comprises the following steps:
(1) prepared by bacterium solution:
1. using activation medium inoculation volume branch Mucor, in 28~30 DEG C, 180~220rmin-1Shaking table on cultivate 1~3
It, is activated；
2. by the volume branch Mucor after activation using expansion breeding culture medium in 28~30 DEG C, 180~220rmin-1Shaking table on vibrate
It carries out within 20~28 hours expanding numerous；
3. being diluted to 1~9 × 10 for numerous volume branch Mucor is expanded with sterile water again7The bacterium solution of CFU/ml to get；
The wherein inoculation and expansion breeding culture medium ingredient are as follows: peptone: 3.0~5.0g/L, yeast extract: 1.0~2.0g/L,
10~20.0g/L of glucose, 1.0~2.0g/L of dipotassium hydrogen phosphate, 0.5~2.5g/L of magnesium sulfate, pH=6.4 ± 0.2；Described
Expand breeding culture medium ingredient are as follows: 3.0~6.0g/L of potato leaching powder, 10~20.0g/L of glucose, agar 10~20.0g/L, pH=
5.6±0.2。
(2) root is infected:
Select black nightshade group, alfalfa group, summer cypress platymiscium as target plant, mode of infection are as follows:
To vegetable seeds vernalization, after germination, transplanting is drenched in the soil of heavy metal pollution, while at transplanting into volume branch
Mucor bacterium solution makes microbial infection plant root, forms Mycorrhizal plant；
Or vegetable seeds is impregnated into diel, it is seeded in the soil of heavy metal pollution, while drenching at sowing into volume branch Mucor
Bacterium solution；Make microbial infection root after plant seed germination growth, forms Mycorrhizal plant；
Wherein Mucor circinelloides liquid adds in the soil at every chitting piece transplanting or at every seed sowing
Entering amount is 10~50mL/m2。
2. according to claim 1 utilize Mycorrhizal plant original position heavy metal pollution restorative procedure, which is characterized in that described
The thickness of sowing of chitting piece or seed is 10~40 plants/m2。
3. according to claim 1 utilize Mycorrhizal plant original position heavy metal pollution restorative procedure, which is characterized in that described
Black nightshade group plant includes: red fruit black nightshade, black nightshade, S. photeinocarpum, the wooden black nightshade.
4. according to claim 1 utilize Mycorrhizal plant original position heavy metal pollution restorative procedure, which is characterized in that described
Alfalfa group plant includes: wild alfalfa, and alfalfa hybridizes clover.
5. according to claim 1 utilize Mycorrhizal plant original position heavy metal pollution restorative procedure, which is characterized in that described
Summer cypress platymiscium includes: Iranian summer cypress, full wing summer cypress, hair flower summer cypress, black wing summer cypress, sharp wing summer cypress, prostrata summercypress, summer cypress.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811101424.1A CN109127722A (en) | 2018-09-20 | 2018-09-20 | It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201811101424.1A CN109127722A (en) | 2018-09-20 | 2018-09-20 | It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN109127722A true CN109127722A (en) | 2019-01-04 |
Family
ID=64815425
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201811101424.1A Pending CN109127722A (en) | 2018-09-20 | 2018-09-20 | It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN109127722A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110653253A (en) * | 2019-09-27 | 2020-01-07 | 兰州大学 | Method for restoring mining area-oasis staggered cadmium contaminated soil |
| CN112574892A (en) * | 2020-12-14 | 2021-03-30 | 浙江理工大学 | Mucor circinelloides for promoting root system development of red sage root and tanshinone synthesis and its use |
| CN113695384A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Plant restoration device and restoration method for soil polluted by heavy metal elements |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445815A (en) * | 2007-11-26 | 2009-06-03 | 北京有容建业科技发展有限责任公司 | Microbial synthesis method of gamma-linolenic acid oil |
| CN103990647A (en) * | 2014-05-12 | 2014-08-20 | 华南师范大学 | Method of enhancing cadmium absorption of black nightshade from soil by utilization of arbuscular mycorrhizal fungi |
| CN104874596A (en) * | 2015-06-17 | 2015-09-02 | 北京中禾葆蓝环保科技有限公司 | Method for repairing soil heavy metal pollution by synergy of plants, mycorrhiza and earthworm |
| CN106424125A (en) * | 2016-07-19 | 2017-02-22 | 山东大学 | Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
-
2018
- 2018-09-20 CN CN201811101424.1A patent/CN109127722A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101445815A (en) * | 2007-11-26 | 2009-06-03 | 北京有容建业科技发展有限责任公司 | Microbial synthesis method of gamma-linolenic acid oil |
| CN103990647A (en) * | 2014-05-12 | 2014-08-20 | 华南师范大学 | Method of enhancing cadmium absorption of black nightshade from soil by utilization of arbuscular mycorrhizal fungi |
| CN104874596A (en) * | 2015-06-17 | 2015-09-02 | 北京中禾葆蓝环保科技有限公司 | Method for repairing soil heavy metal pollution by synergy of plants, mycorrhiza and earthworm |
| CN106424125A (en) * | 2016-07-19 | 2017-02-22 | 山东大学 | Microbial agent for treating heavy metal polluted soil, preparation method and applications thereof |
| CN107127209A (en) * | 2017-07-17 | 2017-09-05 | 中国环境科学研究院 | A kind of method of antimicrobial plant renovation of heavy metal polluted soil with combined |
Non-Patent Citations (1)
| Title |
|---|
| 郝大程 周建强 韩君: "土壤重金属和有机污染物的微生物修复:生物强化和生物刺激", 《生物技术通报》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110653253A (en) * | 2019-09-27 | 2020-01-07 | 兰州大学 | Method for restoring mining area-oasis staggered cadmium contaminated soil |
| CN113695384A (en) * | 2020-05-23 | 2021-11-26 | 中国环境科学研究院 | Plant restoration device and restoration method for soil polluted by heavy metal elements |
| CN113695384B (en) * | 2020-05-23 | 2022-10-25 | 中国环境科学研究院 | Plant restoration device and restoration method for soil polluted by heavy metal elements |
| CN112574892A (en) * | 2020-12-14 | 2021-03-30 | 浙江理工大学 | Mucor circinelloides for promoting root system development of red sage root and tanshinone synthesis and its use |
| CN112574892B (en) * | 2020-12-14 | 2021-09-07 | 浙江理工大学 | Mucor circinelloides for promoting root system development of red sage root and tanshinone synthesis and its use |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106947725B (en) | Microbial agent and application thereof in saline-alkali soil improvement | |
| CN101507409B (en) | Disease-resistant, wireworm-resistant seedling raising matrix and production method thereof | |
| CN103952349B (en) | A Shi genus bacillus and microbiobacterial agent and their application | |
| CN104745483B (en) | A kind of Paecilonyces variotii strain SJ1 and its application | |
| CN102613054B (en) | Method for improving cold and disease resistance of tobacco | |
| CN104911129B (en) | The Bacillus amyloliquefaciens strain and its microbial organic fertilizer of control of plant bacterial wilt | |
| CN110668876B (en) | Composite mycorrhiza biological fertilizer and preparation method and application thereof | |
| CN105964680B (en) | A kind of beach saline land continuous cropping cotton soil ecology renovation agent and the preparation method and application thereof | |
| CN102925358A (en) | Microbial agent and application of same in saline land improvement | |
| CN109652329A (en) | A kind of preparation and application of bacillus solid microbial | |
| CN110106126A (en) | One plant of colloid bacillus cereus and its preparing the application in saline-alkali soil conditioner | |
| CN102240664A (en) | Method for restoring soil polluted by manganese | |
| CN102417413A (en) | Composite microbial fertilizer | |
| CN105475363B (en) | A kind of composite bacteria agent for promoting cucumber growth and its application | |
| CN112538431A (en) | Chlorella pyrenoidosa and biological environment restoration liquid prepared from chlorella pyrenoidosa and application of chlorella pyrenoidosa | |
| CN109127722A (en) | It is a kind of to utilize Mycorrhizal plant original position heavy metal pollution restorative procedure | |
| CN107858293A (en) | A kind of golden yellow basket bacterium and its application | |
| CN109320355A (en) | A kind of saliferous bioactivity conditioner of improvement soil in protected field and its application | |
| CN104789494B (en) | The method for improving turf salt-resistance using garbage compost microbial bacterial agent is strengthened | |
| CN103805535B (en) | The bacillus of a kind of stratosphere and microbial bacterial agent and their application | |
| CN106561422B (en) | A kind of matrix and its application suitable for muskmelon pocket type cultivation | |
| CN106282052B (en) | It is a kind of improve coastal saline soil compound lactobacillus preparation and its application | |
| CN105462885B (en) | One plant of phenolic acid degradation bacteria and its biological organic fertilizer and application | |
| CN107325980A (en) | A kind of radiation hardness series bacillus KH9 and its application in biological antitranspirant | |
| CN108949635B (en) | Bacillus mucilaginosus, microbial fertilizer, preparation method and application thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20190104 |