CN102250784B - Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof - Google Patents
Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof Download PDFInfo
- Publication number
- CN102250784B CN102250784B CN 201110103328 CN201110103328A CN102250784B CN 102250784 B CN102250784 B CN 102250784B CN 201110103328 CN201110103328 CN 201110103328 CN 201110103328 A CN201110103328 A CN 201110103328A CN 102250784 B CN102250784 B CN 102250784B
- Authority
- CN
- China
- Prior art keywords
- phosphate
- phosphorus
- microbial inoculum
- soil
- dgp01
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses an angelica root rhizosphere efficient phosphate-solubilizing bacterium and a microbial inoculum prepared from the same and application thereof. The phosphate-solubilizing bacterium is bacillus sp.DGP01 which is collected in the China General Microbiological Culture Collection Center (Address: Datun Road, Chaoyang District, Beijing, 100101) with the collection number CGMCC No.4755 on April 7, 2011. The microbial inoculum of the phosphate-solubilizing bacterium is prepared by the following steps of: inoculating a bacillus sp.DGP01 strain into a seed culture medium shake flask and performing shaking culturing for an exponential growth phase; inoculating the cultured strain into a seeding tank which is filled with a fermentation culture medium and culturing for an exponential growth phase; and inoculating the fermentation liquor into a production tank which is filled with a fermentation culture medium for culturing. By adopting the phosphate-solubilizing bacterium, slightly-soluble inorganic phosphorus can be transformed into high-quality phosphorus compounds which can be directly absorbed and utilized by plants, the biological effectiveness of slightly-soluble phosphorus in soil can be enhanced, the utilization efficiency of a phosphorus fertilizer is increased, the fertilizer is saved, and the yield is increased; and moreover, the phosphate-solubilizing bacterium plays an important role in culturing and utilizing the ecological fertility of soil and keeping the ecological balance of agriculture, and has an application value.
Description
Technical field
The present invention relates to the biologic product technology field, be specifically related to a kind of angelica root rhizosphere efficient phosphate solubilizing bacteria Bacillus sp.DGP01, and microbial inoculum and the application of fermenting and preparing.
Background technology
Phosphorus is one of necessary mineral element of growth and development of plants, be the important composition compositions such as plant nucleic acid in vivo and plurality of enzymes, coenzyme, ATP, Crop phosphorus amount and its Biomass and yield are remarkable positive correlation, when plant lacks phosphorus, there will be tillers reduces or postpones, heading is postponed, late ripe, and grain number per spike reduces, hollow kernels, corn ear is bald, and rape takes off pod, sweet potato, the potato ball phenomenon such as diminish, so phosphorus is of crucial importance to agriculture production.But there is 74% the scarce phosphorus of arable soil in China, and the phosphorus in soil more than 95% is invalid form, and plant is difficult to directly absorb, and especially the Phosphorus in Red Upland Soil element lacks the most; The accumulation problem of soil phosphorus is quite serious on the other hand, enters phosphorus meeting and the Ca of soil by fertilising
2+, Fe
2+, Fe
3+, Al
3+plasma is in conjunction with forming insoluble phosphate, and the phosphate fertilizer applied has 75%～90% to be accumulated in soil, and the phosphate fertilizer this season utilization ratio of crop generally only has 5%～10%, and most of phosphate fertilizer becomes invalid state (indissoluble state) and accumulates in soil.Due to long-term application of P fertilizer, the potential phosphorus storehouse content of most of agricultural land soils is very large, and provides the phosphorus stream of crop growth very little.In soil, the content of inorganic phosphorus accounts for the 1/3-1/2 of total Phosphorus In Soil amount, and in soil, the form of inorganic phosphorus mainly contains two kinds of primary mineral and secondary minerals, and wherein primary mineral is mainly phosphatic rock, and the main component of phosphatic rock is calcium fluorapatite Ca
5f (PO
4)
3with apatite hydroxide Ca
10(PO
4)
6(OH)
2, secondary mineral mainly refers to the chemical combination state, precipitates the phosphoric acid salt of state.In northerly calcareous soil, calcium phosphate accounts for the 60%-80% of inorganic phosphorus amount, in southern latosol, tertiary iron phosphate and aluminum phosphate account for 80% of inorganic phosphorus amount, and the activity of these bound phosphorus is low, a little less than the soil Phosphorus supply capacity, in soil except inorganic phosphorus, in the phosphorus element, about 1/3-1/2 is organophosphorus, and mainly the form with phytin exists, and accounts for 30%-50%; In addition, use in a large number chemical fertilizer obviously to cause the adverse consequencess such as soil compaction, the decline of soil water-retaining power, grassland degeneration, desertification be serious.Therefore, the utilization ratio of raising phosphorus is the focus that agronomy, soil science, ecotope and microbiological research person pay close attention to always.
Radix Angelicae Sinensis (Angelica sinensis (Oliv.) Diels) is umbelliferae perennial herb medicinal plant, and medicinal part is its dry root, records in each Pharmacopoeia of the People's Republic of China.Radix Angelicae Sinensis has to enrich blood invigorates blood circulation, menstruction regulating and pain relieving, and the effect relaxed bowel, be TCM Gynecology conventional Chinese medicine clinically, have saying of " ten side's rules for doing division with a one-digit divisor on the abacus ".Radix Angelicae Sinensis mainly is distributed in Gansu, Yunnan, Sichuan, Hubei, the provinces and regions such as Guizhou, but main product is in Minxian County, China Gansu, Wudu, the ground such as Cheng County, annual production accounts for the whole nation more than 95%, Radix Angelicae Sinensis is the fertile medicinal plant of a kind of happiness, during nourishing and growing, high fertilizer is to promote root system development, obtain the important factor of high yield, in the Radix Angelicae Sinensis growth and development process to nitrogen, phosphorus, potassium element demand is large, general phosphorus in the soil of Radix Angelicae Sinensis producing region and in farm manure, the potassium deficiency, must rely on fertilising to supplement the deficiency of soil nutrient, and the current main potassium primary phosphate that uses, the chemical fertilizer such as diammonium phosphate and nitrogen-phosphorus-potassium compound fertilizer topdresses.For a long time, people mainly study the activation of soil phosphorus from the chemical conversion processes such as adsorption and desorption, precipitation and dissolving of inorganic phosphorus.Microorganism is very large to conversion and the availability influence of soil phosphorus, and soil microorganisms is the executive that the soil inanimate matter transforms, and is again the active storehouse of plant nutrient, and the biomass of soil microorganisms and turnover thereof play an important role to plant nutrient validity.The phosphorus-solubilizing bacteria of finding up to now mainly concentrates in farm crop soil, and phosphate solubilizing microorganism fertilizer is be applied in the production practice of farm crop also more, and there is not yet relevant report about the microbial ecosystem of the Chinese medicinal materialss such as Radix Angelicae Sinensis cultivation.
Summary of the invention
Goal of the invention: the objective of the invention is in order to solve the deficiencies in the prior art, rhizospheric microorganism to Radix Angelicae Sinensis is furtherd investigate, a kind of degraded soil indissoluble inorganic phosphorus that has is provided, for plant provides the angelica root rhizosphere efficient phosphate solubilizing bacteria Bacillus sp.DGP01 of the high-quality phosphorus element that can directly absorb, another object of the present invention is to provide the phosphorus decomposing microbial inoculum that angelica root rhizosphere efficient phosphate solubilizing bacteria Bacillus sp.DGP01 fermentation prepares, and the application of Bacillus sp.DGP01 in the phosphorus decomposing microbial inoculum of preparation degraded soil indissoluble inorganic phosphorus.
Technical scheme: in order to realize above purpose, the technical scheme that the present invention takes is:
A kind of angelica root rhizosphere efficient phosphate solubilizing bacteria, this phosphate solubilizing bacteria is bacillus sp.DGP01, its preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center is CGMCC No.4755, preservation date: on April 7th, 2011, the preservation name is called: bacillus sp., and the preservation address is: No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City; Strain characteristics is: the thalline rod-short, there is an endogenous spore, and Gram-reaction is positive, aerobic.
The separation method of angelica root rhizosphere efficient phosphate solubilizing bacteria provided by the invention is: gather the Radix Angelicae Sinensis Fresh Plants from the Minxian County, Gansu, shake off the chessom be attached on root, get and stick to the approximately soil of 2～3mm of angelica root surface thickness, be Radix Angelicae Sinensis rhizosphere pedotheque, take the Rhizosphere sampling powder 10g after grinding, in the sterilized water that adds 90ml to contain a little granulated glass sphere, be placed in constant-temperature table with 120 rev/mins of vibrations 30～40 minutes, dilution, get 10
-3with 10
-4dilution soil supension coats that on phosphate-solubilizing bacteria screening solid plate substratum, (substratum consists of: glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, Ca
3(PO
4)
25.0g, agar 20g, distilled water 1000mL, pH 7.0-7.5), at 32 ℃, cultivate 5 days, there is the bacterium colony of molten phosphorus circle to be considered as Decomposing phosphate activity, corresponding bacterial strain is phosphate-solubilizing bacteria, records the size of colony diameter and molten phosphorus circle, and single bacterium colony that the molten phosphorus circle of picking is larger carries out separation and purification, be transferred to the slant medium cultivation and obtain angelica root rhizosphere efficient phosphate solubilizing bacteria bacillus sp.DGP01, in 4 ℃ of preservations.
The microbial inoculum of angelica root rhizosphere efficient phosphate solubilizing bacteria provided by the invention, it prepares by the following method: get Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria Bacillus sp.DGP01 inoculation in the seed culture medium shaking flask, shaking culture is to logarithmic phase; Then above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks that fermention medium is housed (volume ratio that is bacterial classification and fermention medium is 1: 10) by 10% inoculum size, charging capacity is 350～400 liters, is cultured to logarithmic phase; Then 5 tons of production tanks that above-mentioned fermented liquid are equipped with to fermention medium by 10% inoculum size access are cultivated (volume ratio that is fermented liquid and fermention medium is 1: 10), charging capacity is 4.0～4.5 tons, fermentation gained nutrient solution is distributed into the solid fungicide formulation with packing bag after being distributed into liquid dosage form or adopting adsorption by peat by plastic barrel or packing bottle, in fermentation culture thalline quantity can reach 1,000,000,000/more than mL.
As preferred version, the microbial inoculum of above-described angelica root rhizosphere efficient phosphate solubilizing bacteria, described seed culture medium, seeding tank and production tank fermention medium used is made by the raw material of following weight: glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, KH
2pO
41.0g, distilled water 1000mL, the pH of the fermention medium that seed culture medium, seeding tank and production tank are used is 7.0～7.5.
As preferred version, the microbial inoculum of above-described angelica root rhizosphere efficient phosphate solubilizing bacteria, the air flow of sterile air is 1: 0.6～1.0 (being the sterile air that passes in the unit time and the volume ratio of fermention medium) at seeding tank and in producing the fermentation culture process of tank, stirring velocity is 180～200 rev/mins, 30～32 ℃ of culture temperature, incubation time is 48～60 hours.
The preparation method of the microbial inoculum of angelica root rhizosphere efficient phosphate solubilizing bacteria provided by the invention comprises the following steps:
(1) get and separate the Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria Bacillus sp.DGP01 inoculation obtain in the seed culture medium shaking flask, shaking culture is to logarithmic phase;
(2) then get the cultured bacterial classification of step (1) and inoculate in 500 liters of seeding tanks that fermention medium is housed and cultivate by 10% inoculum size, charging capacity is 350～400 liters, is cultured to logarithmic phase;
(3) then getting the cultured fermented liquid of step (2) is equipped with 5 tons of fermention medium by the access of 10% inoculum size and produces in tanks and cultivate, charging capacity is 4.0～4.5 tons, and fermentation gained nutrient solution is distributed into the solid fungicide formulation with packing bag after being distributed into liquid dosage form or adopting adsorption by peat by plastic barrel or packing bottle.
As preferred version, the preparation method of above-described angelica root rhizosphere efficient phosphate solubilizing bacteria microbial inoculum, the described seed culture medium of step (1) wherein, step (2) is made by the raw material of following weight with the described fermention medium of step (3): glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o0.03g, KH
2pO
41.0g, distilled water 1000mL, the pH of seed culture medium and fermention medium is 7.0～7.5.
As preferred version, the preparation method of above-described angelica root rhizosphere efficient phosphate solubilizing bacteria microbial inoculum, the temperature of step (1) shake-flask culture is 28～30 ℃, stirring velocity is 180～200 rev/mins; The air flow that step (2) seeding tank and step (3) are produced sterile air in the fermentation culture process of tank is 1: 0.6～1.0 (being the sterile air that passes in the unit time and the volume ratio of fermention medium), stirring velocity is 180～200 rev/mins, 30～32 ℃ of culture temperature, incubation time is 48～60 hours.
The application of angelica root rhizosphere efficient phosphate solubilizing bacteria provided by the invention in the phosphorus decomposing microbial inoculum of preparation degraded soil indissoluble inorganic phosphorus.
Phosphorus decomposing microbial inoculum provided by the invention can mix use with ground phosphate rock or fertilizer, also can use separately, is applied to the cultivation fertilising of the Chinese medicinal materialss such as Radix Angelicae Sinensis, can improve the yield and quality of Chinese medicinal materials.
Beneficial effect: compared to the prior art angelica root rhizosphere efficient phosphate solubilizing bacteria provided by the invention and the microbial inoculum prepared thereof have the following advantages:
1, the present invention, through the great many of experiments screening, filters out and has degraded insoluble inorganic phosphorus (as Ca from the Radix Angelicae Sinensis rhizosphere
2+, Fe
2+, Fe
3+, Al
3+plasma is in conjunction with forming insoluble phosphate) phosphate solubilizing bacteria Bacillus sp.DGP01, and take phosphate solubilizing bacteria Bacillus sp.DGP01 and cultivate and to prepare the phosphorus decomposing microbial inoculum as strain fermentation, this phosphate solubilizing bacteria and microbial inoculum thereof can be converted into the insoluble inorganic phosphorus high-quality phosphorus element compound that can directly absorb for plant, can improve biological effectiveness and the phosphate fertilizer utilising efficiency of insoluble phosphorus in soil, the fertile volume increase of joint, and can improve Soil structure, improve soil organic matter content, improvement saltings and to cultivating and give full play to the soil ecology fertility, the balance of maintenance agroecological environment etc. all has meaning of crucial importance and using value.
2, phosphate solubilizing bacteria provided by the invention and microbial inoculum thereof, can not only increase the available phosphorus in soil and can promote the absorption of plant to other nutritive elements, and phosphate solubilizing bacteria can adsorb the trace elements such as zinc around root system of plant, copper, calcium, improves plant nutrition; But also can secrete growth regulatory substance, promote root growth; And after the phosphate solubilizing bacteria inoculation, in the breeding of plant rhizosphere raised growth, become the dominant bacteria of plant rhizosphere within for some time, thereby can suppress or reduce the breeding chance of pathogenic micro-organism, improve the resistance against diseases of plant.
3, phosphorus decomposing microbial inoculum provided by the invention can be degraded to the utilizable Soluble Inorganic Phosphorus of plant by insoluble inorganic phosphorus in soil, directly discharge the high-quality phosphatic manure in soil, therefore do not exist in production process to the environmental emission pollutent, phosphate solubilizing microorganism is a kind of environment-friendly type fertilizer truly; And the preparation method of phosphorus decomposing microbial inoculum provided by the invention, production technique is simple, and production efficiency is high, can realize industrialized production, has good economic benefits and social effect.
Embodiment
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment only is not used in and limits the scope of the invention for the present invention is described, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
Embodiment 1
A kind of angelica root rhizosphere efficient phosphate solubilizing bacteria microbial inoculum, it prepares by the following method:
Get Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria Bacillus sp.DGP01 (preserving number is CGMCC No.4755) inoculation in the seed culture medium shaking flask, 30 ℃, 200 rev/mins of kind shaking culture are to logarithmic phase; Then above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks that fermention medium is housed by 10% inoculum size, charging capacity is 400 liters, is cultured to logarithmic phase; Then 5 tons of production tanks that above-mentioned fermented liquid are equipped with to fermention medium by 10% inoculum size access are cultivated, and charging capacity is 4 tons, and fermentation gained nutrient solution directly is distributed into the phosphate solubilizing bacteria microbial inoculum of liquid dosage form by plastic barrel or packing bottle.
Above-described seed culture medium, seeding tank and production tank fermention medium used is made by the raw material of following weight: glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, KH
2pO
41.0g, distilled water 1000mL, pH 7.0～7.5.
Above at seeding tank and in producing the fermentation culture process of tank the air flow of sterile air be 1: 1.0, stirring velocity is 200 rev/mins, 32 ℃ of culture temperature, incubation time is 60 hours.
Embodiment 2
A kind of angelica root rhizosphere efficient phosphate solubilizing bacteria microbial inoculum, it prepares by the following method:
Get Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria Bacillus sp.DGP01 (preserving number is CGMCC No.4755) inoculation in the seed culture medium shaking flask, 30 ℃, 200 rev/mins of kind shaking culture are to logarithmic phase; Then above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks that fermention medium is housed by 10% inoculum size, charging capacity is 450 liters, is cultured to logarithmic phase; Then 5 tons of production tanks that above-mentioned fermented liquid are equipped with to fermention medium by 10% inoculum size access are cultivated, and charging capacity is 4.5 tons, and fermentation gained nutrient solution is be distributed into the phosphate solubilizing bacteria microbial inoculum of solid dosage after adsorption by peat with packing bag.
Above-described seed culture medium, seeding tank and production tank fermention medium used is made by the raw material of following weight: glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, KH
2pO
41.0g, distilled water 1000mL, pH 7.0～7.5.
Above at seeding tank and in producing the fermentation culture process of tank the air flow of sterile air be 1: 0.6, stirring velocity is 180 rev/mins, 30 ℃ of culture temperature, incubation time is 48 hours.
Embodiment 3 phosphate solubilizing bacteria phosphorus decomposing aptitude tests
1, liquid culture method is measured the phosphorus decomposing ability of bacterial strain
The sterilized seed culture medium of 30mL (glucose 10g, (NH pack in the 100mL triangular flask
4)
2sO
40.5g, KCl0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, KH
2pO
41.0g distilled water 1000mL, pH 7.0～7.5), access activated good inclined-plane Bacillus sp.DGP01 bacterial classification (preserving number is CGMCC No.4755), shake-flask culture is to logarithmic phase (approximately 10
8cFUmL
-1).Access respectively phosphorus decomposing nutrient solution (the bottled 40mL substratum of 250mL triangle) by 5% inoculum size, establish the control group that does not connect bacterial classification simultaneously, each is processed and repeats 5 times, after inoculation at 32 ℃, after cultivating 7～10 days on 200 rev/mins of constant-temperature tables, get the 10ml nutrient solution, 12000 rev/mins of centrifugal 10min, then adopt molybdenum antimony resistance colorimetric method to measure supernatant liquor titanium pigment content.
2, sand culture test is measured the phosphorus decomposing ability of bacterial strain
Phosphate solubilizing bacteria Bacillus sp.DGP01 (preserving number is CGMCC No.4755) is inoculated in the aseptic soil or fine sand that are added with insoluble inorganic phosphorus substratum, after cultivating by lab simulation, measure the variation of titanium pigment content in soil (or fine sand) and determine bacterial strain phosphorus decomposing ability.Concrete steps, for getting appropriate river sand, are dried the rear 2mm of mistake sieve, wash 3 times, with the soil except attachment removal, then, after soaking 24h with 1% dilute hydrochloric acid, are washed till neutrality with distilled water, dry for standby.Take fine sand 50g and put into the 50mL triangular flask, add respectively tricalcium phosphate, aluminum phosphate, tertiary iron phosphate and ground phosphate rock 0.5g, 1.05 * 10
5pa sterilizing 30min, sterilizing is 3 times altogether.By in the fine sand of the bacterium liquid of 2mL Bacillus sp.DGP01 bacterium access sterilizing, add 8mL phosphorus decomposing substratum [glucose 10g, (NH
4)
2sO
40.5g, KCl 0.3g, MgSO
47H
2o 0.3g, NaCl 0.3g, FeSO
47H
2o 0.03g, MnSO
44H
2o 0.03g, phosphorus source 5.0g, distilled water 1000mL, pH 7.0-7.5], establish the control group that does not add bacterial classification simultaneously, after 4 weeks, directly use the flooding sand 32 ℃ of cultivations, and adopt molybdenum antimony resistance colorimetric method to measure the content of titanium pigment in vat liquor.
3, results and analysis
Measure the phosphorus decomposing ability of Bacillus sp.DGP01 bacterial strain 3.1 adopt liquid culture method
Bacillus sp.DGP01 bacterial strain all has stronger phosphate solubilization (as table 1) to different insoluble phosphates, especially stronger to the phosphorus decomposing effect of ground phosphate rock and tricalcium phosphate, with respect to control group, improved respectively more than 20 times, the phosphate solubilization of insoluble phosphoric acid aluminium and tertiary iron phosphate is compared with control group and improved respectively more than 7 times with more than 19 times.
The phosphate solubilization of table 1Bacillus sp.DGP01 bacterial strain to different insoluble phosphates
3.2Bacillus the sand culture test result of sp.DGP01 bacterial strain
By Bacillus sp.DGP01 bacterial strain, by sand culture test, concrete experimental result is as shown in table 2:
The sand culture test result of table 2Bacillus sp.DGP01 bacterial strain
Sand culture experimental result by table 2 shows, Bacillus sp.DGP01 bacterial strain provided by the invention all has phosphate solubilization preferably to insoluble phosphorus breeze, tricalcium phosphate, aluminum phosphate and tertiary iron phosphate, can efficiently the insoluble microcosmic salt be converted into to the titanium pigment that plant easily absorbs.
The stability test of embodiment 4Bacillus sp.DGP01 bacterial strain phosphorus decomposing
The present invention adopts the method for continuous passage to investigate Bacillus sp.DGP01 bacterial strain to the insoluble microcosmic salt, as ground phosphate rock, tricalcium phosphate, the stability of the phosphorus decomposing function of aluminum phosphate and tertiary iron phosphate, concrete experimental result is as shown in table 3, result shows that Bacillus sp.DGP01 bacterial strain is to ground phosphate rock after continuous passage 30 times, tricalcium phosphate, the phosphate solubilization of aluminum phosphate and tertiary iron phosphate is still stronger, the titanium pigment content fluctuation range in each generation is all in 10%, experimental result shows that Bacillus sp.DGP01 phosphate solubilizing bacteria provided by the invention has good phosphorus decomposing stability.
The stability test result of table 3Bacillus sp.DGP01 bacterial strain phosphorus decomposing
By above experimental result, show, the present invention screens the Bacillus sp.DGP01 bacterial strain with fine phosphate solubilization obtained from the rhizosphere of Radix Angelicae Sinensis plant, this bacterium can be efficiently by insoluble microcosmic salt in soil, as ground phosphate rock, tricalcium phosphate, aluminum phosphate and tertiary iron phosphate are degraded into the high-quality phosphorus element compound that can directly absorb for plant, thereby can improve biological effectiveness and the phosphate fertilizer utilising efficiency of insoluble phosphorus in soil, the fertile volume increase of joint, and can improve Soil structure, improve soil organic matter content, improvement saltings and to cultivating and give full play to the soil ecology fertility, the balance of maintenance agroecological environment etc. all has meaning of crucial importance and using value, therefore Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria Bacillus sp.DGP01 provided by the invention can be developed into the phosphate solubilizing microorganism fertilizer into the new generation of green environmental protection.
The above is only the preferred embodiment of the present invention; it should be pointed out that for those skilled in the art, under the premise without departing from the principles of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
Claims (2)
1. the microbial inoculum of an angelica root rhizosphere efficient phosphate solubilizing bacteria, it is characterized in that, it prepares by the following method: the DGP01 inoculation is in the seed culture medium shaking flask to get Radix Angelicae Sinensis rhizosphere phosphate solubilizing bacteria-genus bacillus (Bacillus sp.), and shaking culture is to logarithmic phase; Then above-mentioned cultured bacterial classification is inoculated into 500 liters of seeding tanks that fermention medium is housed by 10% inoculum size, charging capacity is 350～400 liters, is cultured to logarithmic phase; Then 5 tons of production tanks that above-mentioned fermented liquid are equipped with to fermention medium by 10% inoculum size access are cultivated, charging capacity is 4.0～4.5 tons, and fermentation gained nutrient solution is distributed into liquid dosage form or adopts the solid fungicide type be distributed into packing bag after adsorption by peat by plastic barrel or packing bottle;
Described seed culture medium, seeding tank and production tank fermention medium used is made by the raw material of following weight: glucose 10g, (NH
4)
2sO
40.5g, KCl0.3g, MgSO
47H
2o0.3g, NaCl0.3g, FeSO
47H
2o0.03g, MnSO
44H
2o0.03g, KH
2pO
41.0g, distilled water 1000mL, and the pH value of seed culture medium, seeding tank and production tank fermention medium used is 7.0～7.5;
Above-described at seeding tank and in producing the fermentation culture process of tank the air flow of sterile air be 1:0.6～1.0, stirring velocity is 180～200 rev/mins, 30～32 ℃ of culture temperature, incubation time is 48～60 hours;
Above-described phosphate solubilizing bacteria is genus bacillus DGP01, and its preserving number at China Committee for Culture Collection of Microorganisms's common micro-organisms center is CGMCCNo.4755, preservation date: on April 7th, 2011.
2. the application of the microbial inoculum of angelica root rhizosphere efficient phosphate solubilizing bacteria claimed in claim 1 in the phosphorus decomposing microbial inoculum of preparation degraded soil indissoluble inorganic phosphorus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110103328 CN102250784B (en) | 2011-04-25 | 2011-04-25 | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN 201110103328 CN102250784B (en) | 2011-04-25 | 2011-04-25 | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN102250784A CN102250784A (en) | 2011-11-23 |
| CN102250784B true CN102250784B (en) | 2013-06-19 |
Family
ID=44978356
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN 201110103328 Expired - Fee Related CN102250784B (en) | 2011-04-25 | 2011-04-25 | Angelica root rhizosphere efficient phosphate-solubilizing bacterium and microbial inoculum prepared from same and application thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN102250784B (en) |
Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107858293B (en) * | 2017-10-11 | 2020-11-24 | 江西农业大学 | Talaromyces aurantiaca and application thereof |
| CN113563130A (en) * | 2021-08-03 | 2021-10-29 | 贵州黔西南喀斯特区域发展研究院(黔西南州农业林业科学研究院) | Preparation method of special phosphorus-release biological bacterial fertilizer for oil tea |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1410528A (en) * | 2002-11-21 | 2003-04-16 | 浙江大学 | Construction method of bacillus phosphorus relieving engineering bacterial strain |
| CN101899405A (en) * | 2010-01-20 | 2010-12-01 | 辛伟成 | Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof |
-
2011
- 2011-04-25 CN CN 201110103328 patent/CN102250784B/en not_active Expired - Fee Related
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1410528A (en) * | 2002-11-21 | 2003-04-16 | 浙江大学 | Construction method of bacillus phosphorus relieving engineering bacterial strain |
| CN101899405A (en) * | 2010-01-20 | 2010-12-01 | 辛伟成 | Bacillus subtilis GU and application in preparing efficient biological compound phosphate fertilizer thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN102250784A (en) | 2011-11-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN102432399B (en) | Biological organic fertilizer for improving stress resistance and output of paddy rice and preparation method thereof | |
| CN103964946B (en) | A kind of paddy rice Special compound microbial fertilizer and preparation method thereof | |
| CN104609979B (en) | A kind of microbial-bacterial fertilizer for improveing salt affected soil and preparation method thereof | |
| CN102876576B (en) | Acidic soil conditioner, and preparation method and application thereof | |
| CN103789223B (en) | Acinetobacter calcoaceticus with free living nitrogen fixation and phosphorus and potassium dissolving capability and application of acinetobacter calcoaceticus | |
| CN103789224B (en) | A kind of edaphic bacillus having from growing nitrogen-fixing, phosphorus decomposing and ability of dissolving potassium and application | |
| CN105295928B (en) | A kind of soil conditioner and preparation method suitable for Degraded Pastures | |
| CN102276367B (en) | Biological-organic-inorganic compound fertilizer and preparation method thereof | |
| CN104609997A (en) | Manufacturing process and application method of ginseng bio-organic fertilizer | |
| CN106591193A (en) | Bacillus amyloliquefaciens with broad spectrum growth-promoting and stress-resisting effects | |
| CN104276882A (en) | Combined fertilizer synergist containing polyglutamic acid, phosphate solubilizing bacteria and potassium bacteria and preparation method and application of combined fertilizer synergist | |
| CN103194405B (en) | Growth-promoting bacteria for promoting ginger growth and preventing and controlling continuous cropping ginger soil-borne wilt and microorganism organic fertilizer produced from growth-promoting bacteria | |
| CN102344812A (en) | Microbiological preparation for improving alkaline land, its preparation method and its application | |
| CN101948780B (en) | Antagonist bacterium for preventing and treating continuous cropping hot pepper epidemic disease and microbial organic fertilizer thereof | |
| CN111499432A (en) | Microbial remediation microbial inoculum for saline-alkali soil as well as preparation method and application of microbial remediation microbial inoculum | |
| CN105385638A (en) | Microbial phosphorus-dissolving preparation and preparation method and application thereof | |
| CN106518362A (en) | Compound microbial fertilizer with continuous cropping resistance and preparation method of fertilizer | |
| CN107353138A (en) | Suitable for the Chemical Mixed Fertilizer and its production method of alkaline land improving | |
| CN104263679A (en) | High-efficiency phosphate-solubilizing bacteria and application thereof | |
| CN109536410A (en) | A kind of salt tolerant growth-promoting composite bacteria agent and its preparation method and application | |
| CN101759501B (en) | Complex microorganisms flora grain type ascharite fertilizer and preparation method thereof | |
| CN103011918A (en) | Microbial organic phosphate fertilizer capable of preventing and controlling pseudo-ginseng blight | |
| CN107628894A (en) | Composite bacteria agent increase soil fertility and its preparation method and application | |
| CN106336329A (en) | Microbial bacterial fertilizer special for honeysuckles and preparation method thereof | |
| CN109182194A (en) | One plant of Yang Ling rhizobium for promoting coronule flower growth and its cultural method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| C17 | Cessation of patent right | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20130619 Termination date: 20140425 |