CN107129951B - Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof - Google Patents
Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof Download PDFInfo
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Abstract
The invention provides a lactobacillus compound preparation capable of remarkably improving the absorptivity of trace elements of peanuts in saline-alkali soil, belonging to the technical field of microorganisms. The active bacteria in the compound Lactobacillus preparation consist of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661, have obvious influence on the growth and metabolism of peanuts in saline-alkali lands, especially can improve the utilization rate of the peanuts in the saline-alkali lands on soil trace elements, and can improve the quality of the peanuts in the saline-alkali lands when applied to the peanut planting process.
Description
Technical Field
The invention relates to a Lactobacillus composite preparation consisting of metabolites of Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661, in particular to a Lactobacillus composite preparation capable of remarkably improving the absorptivity of trace elements of peanuts in saline-alkali soil, and belongs to the technical field of microorganisms.
Background
Trace elements are elements essential to plants but in small amounts, which are not available in the soil or are not utilized by plants, and thus, the plants grow poorly. The Nanjing soil institute of Chinese academy of sciences investigates the contents of national soil trace elements such as zinc, boron, manganese, molybdenum, copper and iron, and the results show that: in most areas of China, trace elements are deficient to different degrees, and the trace elements in soil are in a state of 'moderate deficiency'. The middle and low yield fields in China account for more than 70% of the total cultivated land area, most of the fields have the problem of trace element deficiency, and the cultivated lands lacking trace elements of iron, copper, molybdenum, boron, manganese and zinc account for 5.0%, 6.9%, 21.0%, 46.8%, 34.5% and 51.5% respectively.
The peanut is also called peanut and dicotyledon, the oil content of the seed is up to 50%, the quality is excellent, the smell is fragrant, the peanut is the main oil crop and economic crop in China, the planting area in the oil crop is second to that of rape, the total yield is the first, and the byproduct peanut meal is rich in protein, can be used for poultry, livestock and aquaculture feed, and plays a very important role in China. Meanwhile, peanuts belong to medium salt-tolerant crops, have the characteristics of drought resistance, barren resistance and the like, and can be used as one of the substitute crops of cotton in saline-alkali soil areas. Therefore, the development of the saline-alkali soil peanut planting production has important significance for improving the agricultural planting structure of the saline-alkali soil area, increasing the income of farmers and guaranteeing the safe supply of grain and oil.
However, the availability of trace elements in soil is generally increased under acidic conditions and decreased under alkaline conditions, and saline-alkali soil is more susceptible to the deficiency of trace elements due to the influence of soil conditions. Meanwhile, researches show that mineral nutrient element stress caused by trace element deficiency can aggravate continuous cropping obstacles of peanuts and influence the yield of the peanuts. Therefore, if the utilization rate of the trace elements by the peanuts in the saline-alkali soil can be improved in an effective mode, the method has important significance for improving the quality of the peanuts in the saline-alkali soil.
Disclosure of Invention
The invention aims to determine a compound lactobacillus preparation suitable for being applied to peanuts by researching the influence of different lactobacillus on the growth traits and the quality of the peanuts, thereby providing a lactobacillus compound preparation capable of obviously improving the absorptivity of trace elements of the peanuts in saline-alkali soil and application thereof.
In order to achieve the purpose of the present invention, the inventor does not make extensive efforts through a great number of experimental studies, and finally obtains the following technical scheme: the active bacteria in the compound Lactobacillus preparation consist of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM 8661.
The Lactobacillus plantarum P-8 adopted by the invention is separated from traditional natural fermented yoghurt of inner Mongolia, has excellent capability of resisting gastrointestinal digestive juice and can survive in animal intestinal tracts; the strain is preserved in the China general microbiological culture Collection center, the preservation time is 2012, 06 and 28 days, and the preservation number is CGMCC No. 6312.
The Lactobacillus casei Zhang adopted by the invention is an acid-resistant and bile-acid-resistant probiotic strain separated from the fermented mare milk; the strain is preserved in China general microbiological culture Collection center (CGMCC) at 2006, 4 and 21, and the preservation number is CGMCC No. 1697.
The lactobacillus plantarum CCFM8661 adopted by the invention has probiotics for reducing lead toxicity effect, good acid-base adaptability, good growth under the environment condition of pH 3.0-9.0 and good survival under the environment of pH 2.5. The lactobacillus strain is identified as lactobacillus plantarum (CCFM 18661) by utilizing the microbiological characteristics such as morphological characteristics, culture traits, physiological and biochemical characteristics, and the like, and the lactobacillus strain is preserved in China general microbiological culture Collection center (CGMCC) at 29 th 11 th 2011, and the preservation number is CGMCC No. 5494.
Further preferably, the compound Lactobacillus preparation for improving the utilization rate of the trace nutrients of the peanuts in the saline-alkali soil is prepared by mixing Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 in a proportion of (1.5-2.5): (1.5-2.5): 1 viable bacteria number ratio.
The inventor unexpectedly discovers that the compound bacterial liquid containing Lactobacillus plantarum (P-8), Lactobacillus casei (Zhang) and Lactobacillus plantarum (Lactobacillus) has no obvious inhibiting effect on pathogenic bacteria, but has obvious influence on the growth and metabolism of peanuts, and can improve the utilization rate of the peanuts on trace elements in saline-alkali soil when screening antagonistic bacteria inhibiting the saline-alkali soil peanut pathogenic bacteria. Based on the discovery, the inventor prepares the three bacteria into the compound lactobacillus preparation by inoculating the three bacteria into MRS culture solution for culture and fermentation. Therefore, the invention provides the application of the compound lactobacillus preparation, namely: the compound lactobacillus preparation is applied to improving the utilization rate of trace elements of peanuts in saline-alkali soil; and the application of the compound lactobacillus preparation in the saline-alkali soil peanut planting management.
The compound lactobacillus preparation can be prepared by the following method:
(1) inoculating the Lactobacillus plantarum P-8, the Lactobacillus casei Zhang and the Lactobacillus plantarum CCFM8661 to MRS culture solution according to the inoculation amount of 4 to 10 percent respectively, culturing for 12 to 24 hours at the constant temperature of 35 to 37 ℃, after centrifugal washing, adding mixed solution containing 8 to 12 percent of sterilized defatted milk, 0.5 to 0.6 percent of glucose and 0.5 to 0.8 percent of yeast extract to each thallus precipitate, and adjusting the bacterial count to be more than 2.0 × 109cfu/mL, mixing uniformly and pouring and culturing respectively;
(2) respectively inoculating the Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 strain cultures obtained in the step (1) into an MRS culture solution, culturing at the constant temperature of 35-37 ℃ for 18-24 hours, then respectively inoculating into a mixed solution containing 8-12% of sterilized defatted milk, 0.5-0.6% of glucose and 0.5-0.8% of yeast extract, and culturing at the temperature of 35-37 ℃ for 18-24 hours;
(3) uniformly mixing the bacterial liquids obtained in the step (2) according to the proportion of Lactobacillus plantarum (Lactobacillus plantarum) P-8: Lactobacillus casei (Lactobacillus casei) Zhang: Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 (1.5-2.5): 1, and adding a protective agent into the mixed bacterial liquids, wherein the protective agent comprises 90-120g/L of skimmed milk powder, 25-35mL/L of glycerol, 90-120g/L of maltodextrin, 120-180g/L of lacmise and 8-13 g/LL-sodium glutamate, and the number of the adjusted viable bacteria is more than 2 × 109cfu/mL, pH 5-6.
The compound lactobacillus preparation can be applied to the peanut planting process after being prepared into bacterial liquid, the using amount and the method are that the compound lactobacillus preparation is applied for the first time, the using amount is 2 liters/mu (the total content of viable bacteria is more than 2 × 10) in the peanut sowing period9cfu/mL), if an artificial planting mode is adopted, the fresh water can be diluted after ditching and sprayed into the ditches by an agricultural sprayer, if an all-in-one machine for peanut seeding and film covering is used, the fresh water of the bacterial liquid can be directly diluted and then placed into a pesticide barrel of the all-in-one machine for use, no matter what application mode, the bacterial liquid and the pesticide can not be used together, the use time of the bacterial liquid and the pesticide is more than 2 days, and the bacterial liquid and the pesticide can be applied for the second time in a peanut pod stage with the dosage of 2 liters/mu (the total viable count content is more than 29cfu/mL), spraying or irrigating the diluted solution on the root of the peanut, and carrying out drip irrigation in a drip irrigation mode under a film in a conditional area, wherein the soil is deeply wetted by the irrigation water by 8-10 cm.
Compared with the prior art, the active bacteria in the compound Lactobacillus preparation disclosed by the invention consist of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661, the compound Lactobacillus preparation has obvious influence on the growth and metabolism of peanuts in saline-alkali lands, particularly can improve the utilization rate of the soil trace elements by the peanuts in the saline-alkali lands, and can improve the quality of the peanuts in the saline-alkali lands when being applied to the peanut planting process.
Detailed Description
The foregoing aspects of the present invention are described in further detail below by way of examples, but it should not be construed that the scope of the subject matter of the present invention is limited to the following examples, and that all the technologies realized based on the above aspects of the present invention are within the scope of the present invention. In addition, the experimental methods in the following examples are all conventional methods unless otherwise specified.
Example 1: preparation of composite lactic acid bacteria agent
(1) Inoculating Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM8661 in 6% inoculum size to MRS culture solution, culturing at constant temperature of 35-37 deg.C for 18 hr, centrifuging, washing, adding 10% sterilized defatted milk, 0.6% glucose and 0.8% yeast extract to the thallus precipitate, and regulating the bacteria number to be more than 2.0 × 109cfu/mL, mixed well and poured to culture respectively.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0 g/LL-sodium glutamate.
(3) Respectively inoculating the Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 strain cultures obtained in the step (1) into an MRS culture solution, culturing for 18 hours at constant temperature of 35-37 ℃, then inoculating into a mixed solution of 10% of sterilized skimmed milk, 0.6% of glucose and 0.8% of yeast extract, and culturing for 20 hours at 35-37 ℃.
(4) Uniformly mixing the bacterial liquids obtained in the step (3) according to the proportion of Lactobacillus plantarum (Lactobacillus plantarum) P-8: Lactobacillus casei (Lactobacillus casei) Zhang: Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 of 2:2:1, adding the protective agent prepared in the step (2) into the mixed bacterial liquid, and adjusting the viable count to be more than 2 × 109cfu/mL, pH 5-6.
Example 2: preparation of composite lactic acid bacteria agent
(1) Inoculating Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in 6% inoculating amount to MRS culture solution, culturing at 35-37 deg.C for 18 hr, centrifuging, washing, adding sterilized defatted milk 10%, glucose 0.6% and yeast extract 0.8%, and regulating bacterial count to be greater than 2.0 × 109cfu/mL, mixed well and poured to culture respectively.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0g/L L-sodium glutamate.
(3) Respectively inoculating the Lactobacillus plantarum P-8 and Lactobacillus casei Zhang strain cultures obtained in the step (1) into an MRS culture solution, culturing for 18 hours at a constant temperature of 35-37 ℃, then inoculating into a mixed solution of 10% of sterilized defatted milk, 0.6% of glucose and 0.8% of yeast extract, and culturing for 20 hours at a temperature of 35-37 ℃.
(4) Uniformly mixing the bacterial liquids obtained in the step (3) according to the ratio of Lactobacillus plantarum (P-8) to Lactobacillus casei (Zhang) of 1: 1, adding the protective agent prepared in the step (2) into the mixed bacterial liquid, and adjusting the number of viable bacteria to be more than 2 × 109cfu/mL, pH 5-6.
Example 3: preparation of composite lactic acid bacteria agent
(1) Inoculating Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 and Lactobacillus casei (Lactobacillus casei) Zhang respectively in 6% inoculum size in MRS culture solution, culturing at constant temperature of 35-37 deg.C for 18 hr, centrifuging, washing, adding 10% sterilized defatted milk, 0.6% glucose and 0.8% yeast extract, and regulating bacterial count to be more than 2.0 × 109cfu/mL, mixed well and poured to culture respectively.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0g/L L-sodium glutamate.
(3) Respectively inoculating the Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 and Lactobacillus casei (Lactobacillus casei) Zhang strain cultures obtained in the step (1) into an MRS culture solution, culturing at constant temperature of 35-37 ℃ for 18 hours, inoculating into a mixed solution of 10% of sterilized defatted milk, 0.6% of glucose and 0.8% of yeast extract, and culturing at 35-37 ℃ for 20 hours.
(4) Uniformly mixing the bacterial liquids obtained in the step (3) according to the ratio of Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 to Lactobacillus casei (Lactobacillus casei) Zhang of 1: 2, adding the protective agent prepared in the step (2) into the mixed bacterial liquid, and adjusting the number of viable bacteria to be more than 2 × 109cfu/mL, pH 5-6.
Example 4: test of composite lactic acid bacteria agent in saline-alkali soil peanut planting
The experimental site: rijin county Rizhen Luo Mao Tuo village of Shandong Dongying City
Experiment time: 2016.05-2016.10
Experimental crops: peanut (variety is small blue and white 9)
Experiment soil: moderate saline-alkali soil (soil salt content 2.83g/kg, pH 8.65)
Experimental groups were as follows: the composite lactic acid bacteria prepared in example 2 are applied to the group A of composite lactic acid bacteria, the composite lactic acid bacteria prepared in example 3 are applied to the group B of composite lactic acid bacteria, and the composite lactic acid bacteria prepared in example 1 are applied to the group C of composite lactic acid bacteria.
The application amount and the method are that the first application is carried out, the application amount is 2 liters/mu in the peanut seeding period (the bacterial agents prepared in the embodiments are respectively prepared into bacterial solutions, and the total viable bacteria content of each bacterial solution is 3 × 109cfu/mL), if an artificial planting mode is adopted, after ditching, clear water can be diluted and sprayed into ditches by an agricultural sprayer, if an all-in-one machine for peanut seeding and film covering is used, bacteria liquid clear water can be directly diluted and then placed into a pesticide barrel of the all-in-one machine for use, no matter what application method is adopted, the bacteria liquid and pesticide cannot be used together, the use time of the bacteria liquid and pesticide is more than 2 days, the bacteria liquid is applied for the second time, the consumption is 2 liters/mu in the pod stage of peanuts (the bacteria liquid is prepared by the bacteria agents prepared in each embodiment, and the total viable count content of each bacteria liquid is 3 × 109cfu/mL), diluting with water, spraying or irrigating on peanut rootAnd drip irrigation can be carried out in the conditional region by adopting a drip irrigation mode under the film, and the soil is wetted by the irrigation depth of 8-10 cm.
Planting management: in each group of 1 mu test field, peanuts are planted in a film mulching and ridging mode, the ridge distance is 90cm, 2 rows are arranged on each ridge, the small row distance on each ridge is 35cm, the hole distance is 11cm (2 grains in each hole), and 13474 holes (plants) are formed in each mu. Applying the special fertilizer for peanuts per mu, wherein the nitrogen, phosphorus and potassium content is 45 percent (10: 18: 17), and the weight is 25 kilograms per mu. And no additional fertilizer is applied in the later period.
TABLE 1 comparison of trace element content in peanut leaves under each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus plantarum CCFM8661+ Lactobacillus casei Zhang, group C is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang + Lactobacillus plantarum CCFM 8661. Compared with the blank control group, the composition of the composition,▲p is less than 0.01; the comparison of group C with group A,★p is less than 0.01; the comparison of group C with group B,■P＜0.01。
TABLE 2 comparison of the trace element content in the stems of peanuts under each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus plantarum CCFM8661+ Lactobacillus casei Zhang, group C is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang + Lactobacillus plantarum CCFM 8661. Compared with the blank control group, the composition of the composition,▲p is less than 0.01; the comparison of group C with group A,★p is less than 0.01; the comparison of group C with group B,■P＜0.01。
TABLE 3 comparison of trace element content in peanut needles under each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus plantarum CCFM8661+ Lactobacillus casei Zhang, group CIs lactobacillus plantarum P-8, lactobacillus casei Zhang and lactobacillus plantarum CCFM 8661. Compared with the blank control group, the composition of the composition,▲p is less than 0.01; the comparison of group C with group A,★p is less than 0.01; the comparison of group C with group B,■P＜0.01。
the test results in table 1 show that the content of trace elements such as calcium, iron, zinc, molybdenum and magnesium in peanut leaves can be remarkably increased by 19.0%, 6.4%, 18.9%, 106.6% and 7.2% respectively (table 1) compared with the blank control group, but the content of manganese and copper in the leaves is reduced by 9.4% and 7.7% respectively compared with the blank control group.
The test results in the table 2 show that the content of trace elements such as calcium, iron, manganese, copper, zinc, molybdenum and magnesium in the stem of the peanut can be remarkably increased by the composite bacteria consisting of lactobacillus plantarum P-8, lactobacillus casei Zhang and lactobacillus plantarum CCFM8661 compared with the blank control group, lactobacillus plantarum P-8+ lactobacillus casei Zhang in the group A and lactobacillus plantarum CCFM8661+ lactobacillus casei Zhang in the group B, and is respectively increased by 55.1%, 14.7%, 18.3%, 33.7%, 10%, 74.4% and 30.5% compared with the blank control group.
The test results in the table 3 show that the content of trace elements such as calcium, iron, manganese, copper, zinc, molybdenum and magnesium in the peanut pins can be remarkably increased by the composite bacteria consisting of lactobacillus plantarum P-8, lactobacillus casei Zhang and lactobacillus plantarum CCFM8661 compared with the blank control group, lactobacillus plantarum P-8+ lactobacillus casei Zhang of the group A and lactobacillus plantarum CCFM8661+ lactobacillus casei Zhang of the group B, and is respectively increased by 9.1%, 19.4%, 29.8%, 5.9%, 32.3%, 24.2% and 5.2% compared with the blank control group.
In a word, through statistical comparison, the group C has very significant difference compared with the group A and the group B in the content of a plurality of trace elements, which shows that the lactobacillus plantarum P-8, the lactobacillus casei Zhang and the lactobacillus plantarum CCFM8661 can synergistically improve the utilization rate of the saline-alkali soil peanuts on the trace elements in the soil.
Claims (7)
1. The application of the compound Lactobacillus preparation in improving the utilization rate of trace elements in the peanuts in the saline-alkali soil is characterized in that active bacteria in the compound Lactobacillus preparation consist of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM 8661.
2. The application of the compound Lactobacillus preparation in improving the utilization rate of trace elements in peanuts in saline-alkali soil according to claim 1, wherein the active bacteria in the compound Lactobacillus preparation are prepared from Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 (1.5-2.5): (1.5-2.5): 1 viable bacteria number ratio.
3. A peanut planting method for improving utilization rate of trace elements of peanuts in saline-alkali soil is characterized by comprising the step of applying a bacterial liquid prepared from a compound lactobacillus preparation to a peanut sowing period for the first time, wherein the dosage is 2-3 liters per mu, and the total number of viable bacteria is more than 2 × 109cfu/mL, the dosage of the second application in the pod stage of peanut is 2-3L/mu, and the total number of viable bacteria is more than 2 × 109cfu/mL, after being diluted by water, the cfu/mL is sprayed or irrigated or dripped on the root of the peanut; the active bacteria in the compound Lactobacillus preparation consist of Lactobacillus plantarum P-8, Lactobacillus casei Zhang and Lactobacillus plantarum CCFM 8661.
4. The peanut planting method for improving the utilization rate of trace elements in peanuts in saline-alkali soil according to claim 3, wherein the active bacteria in the compound Lactobacillus preparation are prepared from Lactobacillus plantarum (Lactobacillus plantarum) P-8, Lactobacillus casei (Lactobacillus casei) Zhang and Lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 (1.5-2.5): (1.5-2.5): 1 viable bacteria number ratio.
5. The peanut planting method for improving the utilization rate of the trace elements in the peanuts in the saline-alkali soil according to claim 3, wherein if an artificial planting mode is adopted, the bacterial liquid is diluted by adding clear water after ditching, and is sprayed into the ditches by adopting an agricultural sprayer.
6. The peanut planting method for improving the utilization rate of trace elements in peanuts in saline-alkali soil according to claim 3, wherein if a peanut sowing and film mulching all-in-one machine is adopted, the bacterial liquid is directly diluted with clear water and then placed in a pesticide barrel of the all-in-one machine for use.
7. The peanut planting method for improving the utilization rate of the trace elements in peanuts in saline-alkali soil according to any one of claims 3 to 6, wherein the bacterial liquid and the pesticide cannot be used together, and the time interval between the use of the bacterial liquid and the use of the pesticide is more than 2 days.
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| CN112970547A (en) * | 2021-02-05 | 2021-06-18 | 南阳市经济作物技术推广站 | Peanut planting method for relieving peanut continuous cropping obstacle |
| CN114774403B (en) * | 2022-04-27 | 2023-08-08 | 至农科技发展(浙江)有限公司 | Targeted breeding method for agricultural lactic acid bacteria |
| CN115960730A (en) * | 2023-01-13 | 2023-04-14 | 青岛农业大学 | Fungus and bacterium composite preparation and application thereof in promoting peanut growth and salt resistance |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013127146A1 (en) * | 2012-02-28 | 2013-09-06 | 江南大学 | Lactobacillus plantarum able to relieve lead toxicity and use thereof |
| CN106222108A (en) * | 2016-08-10 | 2016-12-14 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | For promoting compound lactobacillus microbial inoculum and the preparation method of grape quality |
| CN106259459A (en) * | 2016-07-22 | 2017-01-04 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactic acid bacteria compound formulation improving potato starch and dry matter content and application thereof |
| CN106262119A (en) * | 2016-08-30 | 2017-01-04 | 宁夏沃福百瑞枸杞产业股份有限公司 | The preparation method of high dietary-fiber probiotic bacteria Fructus Lycii sheet |
| CN106282052A (en) * | 2016-08-08 | 2017-01-04 | 青岛农业大学 | A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof |
-
2017
- 2017-06-19 CN CN201710462154.6A patent/CN107129951B/en active Active
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2013127146A1 (en) * | 2012-02-28 | 2013-09-06 | 江南大学 | Lactobacillus plantarum able to relieve lead toxicity and use thereof |
| CN106259459A (en) * | 2016-07-22 | 2017-01-04 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactic acid bacteria compound formulation improving potato starch and dry matter content and application thereof |
| CN106282052A (en) * | 2016-08-08 | 2017-01-04 | 青岛农业大学 | A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof |
| CN106222108A (en) * | 2016-08-10 | 2016-12-14 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | For promoting compound lactobacillus microbial inoculum and the preparation method of grape quality |
| CN106262119A (en) * | 2016-08-30 | 2017-01-04 | 宁夏沃福百瑞枸杞产业股份有限公司 | The preparation method of high dietary-fiber probiotic bacteria Fructus Lycii sheet |
Non-Patent Citations (4)
| Title |
|---|
| Mg2+ improved the thermotolerance of probiotic lactobacillus rhamnosus GG,Lactobacillus casei Zhang and lactobacillus plantarum P-8.;Yang Y等;《Letters in applied microbiology》;20170227;第64卷;全文 * |
| The binding characters study of lead removal by lactobacillus plantarum CCFM8661.;Ruijie Yin等;《Eur food res technol》;20160311;第242卷;摘要 * |
| 益生乳酸菌Lactobacillus casei Zhang和Lactobacillus plantarum P8对全价饲料pH及微生物类群变化的研究;魏爱彬等;《微生物学杂志》;20120331;第32卷(第2期);摘要，结论 * |
| 缓解铅毒性植物乳杆菌CCFM8661的微生物学性质及其应用的研究;田丰伟等;《食品安全质量检测学报》;20140430;第5卷(第4期);摘要 * |
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