CN102308853A - The biological seed coating agent of control soybean SCN - Google Patents
The biological seed coating agent of control soybean SCN Download PDFInfo
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- CN102308853A CN102308853A CN201110049232A CN201110049232A CN102308853A CN 102308853 A CN102308853 A CN 102308853A CN 201110049232 A CN201110049232 A CN 201110049232A CN 201110049232 A CN201110049232 A CN 201110049232A CN 102308853 A CN102308853 A CN 102308853A
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- coating agent
- seed coating
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Abstract
The present invention relates to a kind of biological seed coating agent of preventing and treating the soybean SCN, it comprises line active substance, the element that is mixed in proportion and the various auxiliary agent extremely of 2 kinds of biocontrol bacterial strains.A large amount of evidences, biological seed coating agent of the present invention all meets kind of the requirement of clothing agent on physicochemical character, obviously promote the growth of soybean seedling behind the dressing, improves soybean yields, and is remarkable to the control effect of soybean seedling SCN disease.
Description
Technical field:
The invention belongs to the microbial pesticide technical field, be specifically related to a kind of biological seed coating agent of controlling the soybean SCN.
Background technology:
Soybean cyst nematode (Soybean Cyst Nematode) is claimed soybean verticillium wilt, flue seedling sprout again, is one of primary disease of restriction world soybean production.This disease generally makes the soybean underproduction 10%～30%, and serious plot can reach 70%～90%, even causes total crop failure, and is all bigger than the harm that any single disease caused.This disease has become one of main disease in soybean main producing region expansion rapidly, from world wide, and the harm of soybean cyst nematode and spread the trend that increases the weight of is day by day arranged.In recent years along with the continuous expansion of China's soybean culture area; The distribution of soybean SCN (Heterodera glycines) is also more and more wider; Mainly occurring in totally 14 provinces, cities and autonomous regions such as northeast and the Yellow River and Huai He River sea, is the one of the main reasons that causes the soybean low-yield land.
Control soybean cyst nematode evil does not have special effective method at present; Because of containing carbofuran effective insecticidal compositions such as (furans are red) the soybean SCN there is certain control efficiency in the domestic kind clothing agent commonly used; But along with the red forbidding of furans, seeking new substitute becomes inevitable.Especially over past 20 years, along with the development of sustainable agriculture and organic farming, the focus that people will prevent and treat nematodiasis has turned to biological control.In the process that microbial pesticide develops rapidly, some new bio technology companys arise at the historic moment like Mycogen company, Ecogen company etc. in the world.Some global large-scale chemical pesticide company and biotech companies also invest heavily in microbial pesticide one after another like the E.I.Du Pont Company of the U.S. and Monsanto Company, the Beyer Co., Ltd of Germany, the Syngenta Co.,Ltd of Switzerland, the Sumitomo company of Japan etc.
In the middle of nearly ten thousand kinds of antibiotic finding at present, have 70% to be the various secondary metabolites that produce by actinomycetes approximately.Farm antibiotics has advantages such as more efficient, quick, safe as the important monoid in the biopesticide than other biopesticide.The secondary metabolite that actinomycetes produce has the abundant structures diversity, so actinomycetes show great potential as the biological and ecological methods to prevent plant disease, pests, and erosion factor of nematode.In addition, the PGPR preparation is that the collection of on the single fertilizer efficiency research basis of rhizobia fertilizer and composite biological fertilizer, developing is in recent years urged to give birth to, disease-resistant biological and ecological methods to prevent plant disease, pests, and erosion is the novel live body Inoculant of one.Discover that PGPR is in the gathering of plant rhizosphere, their vigorous metabolism have been strengthened the decomposition of organic substance in the soil, have promoted the mineralising of plant nutrient, have strengthened the supply to crop alimentary.The plant hormone that some PGPR bacterial strain produces can promote plant root growth, enhance root system to absorb the ability of mineral nutrition and moisture, thereby promotes plant growing.Simultaneously PGPR rhizosphere decide to grow can suppress to a certain extent pathogen decide grow and propagate, and the growing of promotion mycorrhizal fungi, and polluted soil is also had certain biological restoration ability.Therefore; The present invention is intended to utilize bacillus megaterium among actinomycetic antibiotic activity and the PGPR to the biological control effect of plant nematode; Thereby form the harm that the diversity microorganism species is effectively controlled the soybean SCN, for new approach is opened up in the biological control of China plant nematode.
It is relatively weaker that clothing agent market is planted by China at present, and kind is single, and former medicine is few.Existing kind of clothing agent occupies the majority with the kind clothing agent of the formulations of pesticide, and to plant clothing agent Central Plains medicine kind serve as mainly to prevent and treat soil insect with high-toxic pesticide furans pellet, and new insecticide pesticide kind is few.On the low side, film forming agent of active component total content and auxiliary agent research is not enough in kind of the clothing agent prescription, and selectable kind is few.Along with people's is to the consideration of various safety factors, and biological seed coating agent becomes the focus of research just gradually.Through the screening of biocontrol bacterial strain, find bacterial strain efficiently, utilize again the bio-diversity principle with many strains biocontrol bacterial strain through the synergistic function that is mixed, be applied to biological seed coating agent, thereby reach enhancing crop anti-adversity ability, disease-resistant worm and short effect of giving birth to raising the output.
Bacterial strain Snea253 is the strain biological and ecological methods to prevent plant disease, pests, and erosion actinomycetes (patent No.: ZL200810010465.X that this seminar separates from soil; Culture presevation number is CGMCC No.2348); Obtained the national inventing patent mandate on July 7th, 2010, found the nematocidal active material ability good restraining in this bacterial strain fermentation liquor and kill the soybean SCN through test; Bacterial strain Sneb207 promotes soybeans they grow for the isolated strain of this seminar and can suppress the PGPR of soybean SCN; Be bacillus megaterium Bacillus megaterium; Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 9th, 2008, preserving number is CGMCC No.2447.The zymotic fluid of this 2 bacterial strain biocontrol microorganisms is mixed, promptly reaches the breeding effect that suppresses the soybean SCN, can promote the soybean plant strain healthy growth again, improve output.
Summary of the invention:
The objective of the invention is to obtain a kind of biological seed coating agent that can suppress soybean cyst nematode evil promotion soybeans they grow; According to the bio-diversity principle; With many strains have the active bacterial strain of line extremely by the conventional liq fermented and cultured after, use the metabolite and the processing that are mixed such as other elements, auxiliary agent of this bacterium, the formation biological seed coating agent; In order to the dressing soya seeds, reach the purpose that effective control soybean cyst nematode evil promotes the soybean seedling growth.
The present invention is through discovering; Soil actinomycetes and bacterium have many types all to have certain activity of line extremely, from these biocontrol bacterial strains, filter out composite effective combination streptomyces venezuelae (Streptomyces venezuelae) Snea253 and bacillus megaterium (Bacillus) B207 and further test.
Biocontrol bacterial strain streptomyces venezuelae of the present invention (Streptomyces venezuelae) Snea253 and bacillus megaterium (Bacillus) Sneb207 after cultivating through test tube strains, prepare through enlarging fermented and cultured again, and its concrete grammar is following:
1. test tube strains is cultivated
Actinomycetes seed culture based formulas: soluble starch 20g, dipotassium hydrogen phosphate 0.5g, potassium nitrate 1g, sodium chloride 0.5g, ferrous sulfate 0.01g, epsom salt 0.5g, agar 15-20g adds water to 1000ml, and pH is 7.2-7.4.The Snea253 that is inoculated on the inclined-plane was placed 28 ℃ of constant temperature culture 5～7 days.
The bacterial culture based formulas is beef extract-peptone (NA) medium, i.e. composition: beef extract 3g, and peptone 5g, yeast extract 1g, sucrose 10g, agar 15g adds water to 1000ml.The Sneb207 that is inoculated on the inclined-plane is placed 25 ℃ of constant temperature culture 2d, obtain test tube strains.
2. liquid fermentation and culture
Actinomycetes liquid fermentation medium prescription is: analysis for soybean powder 4%, glucose 2%, soluble starch 6%, ammonium sulfate 0.4%, sodium chloride 0.2%, dipotassium hydrogen phosphate 0.02%, calcium carbonate 0.2%.
The bacterial liquid fermentation medium is liquid NA medium, and filling a prescription is: by weight percentage, contain beef extract 0.2%, peptone 1%, yeast extract 0.5%, sucrose 1%, remainingly be distilled water that pH is 8.
Test tube strains is inoculated in 250mL triangular flask (the every bottled 100mL) liquid nutrient medium, and under 25 ℃～28 ℃, shaking speed is with 150rmin
-1～200rmin
-1, shaken cultivation 48h, obtain seed culture fluid.
The bacterial classification of liquid fermentation can carry out large-scale production through large fermentation tank, and its product is the manufactured goods of zymotic fluid or zymotic fluid.
Embodiment:
In order further to illustrate in more detail rather than, to have provided the following example in order to limit the present invention.
Embodiment 1: the fermentation of biocontrol microorganisms
The bacterial classification inoculation of the liquid culture of streptomyces venezuelae (Streptomyces venezuelae) Snea253 ferments to the fermentation tank of 50L-100L, cultivates down at 28 ℃, and initial pH is 6, fermentation 120h-140h.The bacterial classification inoculation of the liquid culture of bacillus megaterium (Bacillus) Sneb207 ferments to the fermentation tank of 50L-100L, cultivates down at 25 ℃, and pH is 8, fermentation 48h-72h.Survey sample the activity of zymotic fluid during this time, confirm to put a jar time according to active calibration curve to the target nematode.
Embodiment 2: the screening of element consumption
Agents useful for same all is that analysis is pure, comprises potassium dihydrogen phosphate, potassium sulphate, copper sulphate, manganese sulphate, ferrous sulfate, ammonium molybdate, boric acid etc.Each element being mixed the back according to different proportion grind, add binding agent to the soybean dressing, is screening index with the germination vigor of soya seeds, selects the macroelement that promotes soybeans they grow and the combination of trace element.Combination matching (by weight percentage): CuSO
40.09%, (NH
4)
6Mo
7O
244H
2O 0.18%, H
3BO
30.04%, MnSO
40.3%, FeSO
40.04%, KH
2PO
41.5%, K
2SO
41%.
Embodiment 3: the biological seed coating agent preparation of control soybean SCN
After each biocontrol microorganisms fermentation liquor treatment, in ratio of the present invention carry out composite after, more various elements are mixed the back in proportion and pulverize to grind; Mix adding distilled water with auxiliary agent again and stir into uniform viscous fluid; Wet grinding, vibration mixes, and prepares biological seed coating agent of the present invention.
Embodiment 4: the field effect of biological seed coating agent
Carried out sub-district, field sowing test in Kangping County, Liaoning Province and Heilongjiang Province's Anda City in 2010.Small plot experiment is taked the method for 5 repetitions of district's group at random.Each handles 5 row districts, and 2.5m is capable long, and line-spacing 0.65m handles 1m at interval, sub-district area 8.1m
2, gross area 450m
2With dressing not is blank, is kind of a clothing agent contrast with the agent of commodity kind clothing.
Through cyst number and root interior lines borer population on the counting soybean root, field species test result shows of the present invention respond well, has not only controlled the propagation of soybean SCN quantity, and has promoted the growth of soybean seedling and the increase of soybean yields.Result of the test such as following table.
Table 1 soybean biological seed coating agent is handled the variation of outer cyst of soybean root and Gen Nei nematode population
Biological seed coating agent of the present invention compares the cyst inhibiting rate at the preventive effect of Kangping and grand celebration and blank and has reached 56.63% and 64.24% respectively, significant difference.The root interior lines borer population of biological seed coating agent of the present invention is compared with blank, and nematode inhibiting rate in the root has been reached 63.16% and 80.95% respectively.Biological seed coating agent of the present invention can significantly suppress the propagation of cyst quantity in the soil, compares difference with blank and reaches utmost point significance level, and inhibiting rate reaches 56.1% and 60.3% respectively, also reaches utmost point significance level with commodity kind clothing agent contrast.
Table 2 soybean biological seed coating agent is to the influence of plant strain growth
Through data analysis, can see seedling stage biological seed coating agent of the present invention can promote the growth of soybean seedling safely and effectively; Product work is surveyed in species test in that the soybean maturing stage has been carried out plant height, single-strain legumen number, single-strain grain number and 100-grain weight etc. to several processing of grand celebration test basic point setting.Measurement data (table 2) shows: the soybean plant height does not have significant difference, but single-strain legumen number compares with blank that all there were significant differences, and the average single-strain legumen number of biological seed coating agent of the present invention is at most suitable with disease-resistant variety; Individual plant pod grain number and 100-grain weight also are significantly higher than blank and commodity kind clothing agent contrast.Explain that biological seed coating agent of the present invention not only can be good at suppressing the breeding of soybean SCN, but also can promote soybean growth and 100-grain weight increase and reach the raising the output purpose.
Claims (6)
1. biological seed coating agent, it comprises microbial secondary metabolite, trace element and the macroelement harmless to plant, and other auxiliary agents, can suppress the soybean cyst nematode evil and the short production-increasing function of giving birth to is arranged.
2. according to the biological seed coating agent of claim 1; Wherein secondary metabolite (is streptomyces venezuelae Streptomyces venezuelae from actinomycetes strain Snea253; Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on January 18th, 2008; Preserving number is CGMCC No.2348) and the short spore bacillus Sneb207 of sprouting of rhizosphere (be bacillus megaterium Bacillus megaterium; Be deposited in China Committee for Culture Collection of Microorganisms common micro-organisms center on April 9th, 2008, preserving number is CGMCC No.2447).
3. according to the biological seed coating agent of claim 1 and claim 2, the content of microorganisms of this biological seed coating agent is 10
8Cfu/ml.
4. according to the biological seed coating agent of claim 1, wherein the proportioning of trace element and macroelement is CuSO
40.09%, (NH
4)
6Mo
7O
244H
2O 0.18%, H
3BO
30.04%, MnSO
40.3%, FeSO
40.04%, KH
2PO
41.5%, K
2SO
41%.
5. according to the biological seed coating agent of claim 1; Wherein auxiliary agent comprises that the weight ratio that the film forming agent methylcellulose accounts for described biological seed coating agent is 4%-6%; It is 2%-4% that the dispersant lignin sulfonic acid accounts for weight ratio, and it is 1% that the binding agent sodium carboxymethylcellulose accounts for weight ratio.
6. the biological seed coating agent according to claim 1 evenly is wrapped in the soybean surface by 1% of grain weight, can sow after drying, and is applicable to the control of soybean SCN.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110049232A CN102308853A (en) | 2011-03-02 | 2011-03-02 | The biological seed coating agent of control soybean SCN |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201110049232A CN102308853A (en) | 2011-03-02 | 2011-03-02 | The biological seed coating agent of control soybean SCN |
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ID=45422721
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| CN201110049232A Pending CN102308853A (en) | 2011-03-02 | 2011-03-02 | The biological seed coating agent of control soybean SCN |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
| CN108651523A (en) * | 2018-04-28 | 2018-10-16 | 湖北省生物农药工程研究中心 | Applications of the streptomycete NBF715 of one plant of separation in preparing Plant nematode insecticide |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1621514A (en) * | 2003-11-28 | 2005-06-01 | 中国农业科学院生物防治研究所 | Lichen bacillus strain (B-0A12) and preparation thereof |
| CN101225370A (en) * | 2008-02-25 | 2008-07-23 | 沈阳农业大学 | Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof |
| CN101723763A (en) * | 2009-10-31 | 2010-06-09 | 黑龙江八一农垦大学 | Soybean biological seed coating agent |
-
2011
- 2011-03-02 CN CN201110049232A patent/CN102308853A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1621514A (en) * | 2003-11-28 | 2005-06-01 | 中国农业科学院生物防治研究所 | Lichen bacillus strain (B-0A12) and preparation thereof |
| CN101225370A (en) * | 2008-02-25 | 2008-07-23 | 沈阳农业大学 | Preparation method of streptomyces having activity for poisoning plant nematodes and uses thereof |
| CN101723763A (en) * | 2009-10-31 | 2010-06-09 | 黑龙江八一农垦大学 | Soybean biological seed coating agent |
Non-Patent Citations (1)
| Title |
|---|
| 孙华等: "大豆根际促生菌Sneb207对不同种类线虫毒性的研究", 《大豆科学》 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102925387A (en) * | 2012-10-26 | 2013-02-13 | 沈阳农业大学 | Bacillus simplex for inducing soybean to generate soybean cyst nematode resistance and application |
| CN105494447A (en) * | 2015-12-23 | 2016-04-20 | 沈阳农业大学 | Biological seed coating agent for controlling soybean root disease and preparation method and application thereof |
| CN105494447B (en) * | 2015-12-23 | 2018-11-02 | 沈阳农业大学 | Biological seed coating agent and its preparation method and application for controlling soybean root disease |
| CN108651523A (en) * | 2018-04-28 | 2018-10-16 | 湖北省生物农药工程研究中心 | Applications of the streptomycete NBF715 of one plant of separation in preparing Plant nematode insecticide |
| CN108651523B (en) * | 2018-04-28 | 2020-05-05 | 湖北省生物农药工程研究中心 | Application of separated streptomyces NBF715 in preparation of plant nematode pesticide |
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Application publication date: 20120111 |