CN107129952B - Lactobacillus composite microbial agent for improving yield and characters of peanuts in saline-alkali soil and application thereof - Google Patents
Lactobacillus composite microbial agent for improving yield and characters of peanuts in saline-alkali soil and application thereof Download PDFInfo
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Abstract
The invention provides a Lactobacillus composite microbial inoculum for improving the quality and yield traits of peanuts in saline-alkali soil and application thereof, wherein active bacteria in the Lactobacillus composite microbial inoculum comprise Lactobacillus bifidus V9(Bifidobacterium animalis subsp. Lactis V9), Lactobacillus plantarum P-8 and Lactobacillus casei Zhang. The lactobacillus composite microbial inoculum has obvious influence on the growth and metabolism of the peanuts in the saline-alkali land, and particularly can improve the quality and yield characters of the peanuts in the saline-alkali land, thereby providing a relatively ideal bacterial fertilizer raw material for improving the quality and yield of the peanuts in the saline-alkali land.
Description
Technical Field
The invention relates to a Lactobacillus composite microbial inoculum capable of remarkably improving the quality and yield characters of peanuts in saline-alkali soil, in particular to a Lactobacillus composite microbial inoculum consisting of Lactobacillus bifidus V9(Bifidobacterium animalis subsp. Lactis V9), Lactobacillus plantarum P-8 and Lactobacillus casei Zhang and metabolites thereof, and belongs to the technical field of microorganisms.
Background
The peanut is also called peanut and dicotyledon, the vein is reticular vein, and the seed is coated by peanut peel. Historically, it has been called Changsheng fruit, Didou, Faghua ginseng, Laodisong, Chenshou fruit, Mucuna, Fig, Diguo, Tangren bean. Peanuts are good at nourishing and tonifying and are helpful for prolonging life, so they are also called "healthy fruits" by folk, and are known as "vegetable meat" and "meat in vegetable" as soybeans. The peanut is one of five oil crops in the world, is an economic crop for both eating and oil extraction, plays an important role in world agricultural production and trade, is also an important oil crop and economic crop, has the oil content of 50 percent, excellent quality and fragrant smell, is second to rape in the planting area of the oil crop, has the first total yield, is rich in protein in the byproduct peanut meal, can be used for poultry, livestock and aquaculture feed, and plays a very important role in China. Therefore, the development of the peanut industry has important significance for increasing the income of farmers and guaranteeing the national oil safety.
Land salinization has become a worldwide resource and environment problem. According to statistics, the area of the global saline-alkali soil reaches 9.55 multiplied by 108hm2. The phenomenon of soil salinization in China is more and more serious, and according to incomplete statistics of textbook organization and grain and agriculture organization of the United nations, the area of the saline-alkali soil in China is about 9.91 multiplied by 107hm2Most of them are under development. Peanuts belong to medium salt-tolerant crops, have the characteristics of drought resistance, barren resistance and the like, can be used as one of substitute crops of cotton in saline-alkali soil areas, and has important significance for improving agricultural planting structures in the saline-alkali soil areas, increasing the income of farmers and ensuring the safe supply of grain and oil in the development of saline-alkali soil peanut production.
However, the bad soil environment of the saline-alkali soil seriously damages the growth of peanut root systems in the soil, various trace elements in the salinized soil are insufficient, the peanut crops are caused to grow thin and thin seedlings, the surface of the salinized soil is hardened, the peanut fruits are small, and the yield is reduced. At present, an effective technical means for increasing the yield of peanuts in saline-alkali soil still does not exist.
Disclosure of Invention
The invention aims to determine a compound lactobacillus preparation suitable for being applied to peanuts by exploring the influence of different lactobacillus on the yield and quality of the peanuts, thereby providing a lactobacillus compound preparation capable of remarkably improving the quality and yield and quality of the peanuts in saline-alkali soil and application thereof.
In order to achieve the purpose of the invention, the inventor makes continuous efforts through a large number of experimental researches to finally obtain a mixed microbial inoculum, and the microbial inoculum is prepared into a bacterial solution and irrigates the root of peanuts, so that the quality and the yield characters of the peanuts in the saline-alkali soil can be obviously improved. Specifically, the technical aspects of the present invention are summarized as follows:
the active bacteria in the Lactobacillus composite microbial inoculum consist of Lactobacillus bifidus V9(Bifidobacterium animalis subsp. Lactis V9), Lactobacillus plantarum P-8 and Lactobacillus casei Zhang.
It should be noted that lactobacillus bifidus V9(Bifidobacterium animalis sp. lactentis V9) used in the present invention is an acid-and bile salt-resistant probiotic isolated from the intestinal tract of healthy mongolian children, and has the effects of inhibiting harmful bacteria, protecting intestinal mucosa barrier, and metabolizing to produce organic acids, and has a high survival rate in an environment of pH 2.0-8.0. The strain is classified as Bifidobacterium animalis subsp.lactis, and has been preserved in China general microbiological culture Collection center (CGMCC No. 5470) at 11/18 days 2011.
The Lactobacillus plantarum P-8 adopted by the invention is separated from traditional natural fermented yoghurt of inner Mongolia, has excellent capability of resisting gastrointestinal digestive juice and can survive in animal intestinal tracts; the strain is preserved in the China general microbiological culture Collection center, the preservation time is 2012, 06 and 28 days, and the preservation number is CGMCC No. 6312.
The Lactobacillus casei Zhang adopted by the invention is an acid-resistant and bile-acid-resistant probiotic strain separated from the fermented mare milk; the strain is preserved in China general microbiological culture Collection center (CGMCC) at 2006, 4 and 21, and the preservation number is CGMCC No. 1697.
Further preferably, the Lactobacillus composite inoculant for improving the peanut quality and yield traits of the saline-alkali soil as described above is prepared from Lactobacillus bifidus (Bifidobacterium animalis subsp.lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei (Lactobacillus casei) Zhang in a ratio of 1: (2-3): (2-3) the ratio of viable bacteria number.
The inventor unexpectedly discovers that the composite bacterial liquid containing Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum (Lactobacillus plantarum) P-8 and Lactobacillus casei (Lactobacillus casei) Zhang has no obvious inhibition effect on pathogenic bacteria, but has obvious influence on growth and metabolism of peanuts, and can improve the quality and yield characteristics of the peanuts when screening antagonistic bacteria inhibiting the peanut pathogenic bacteria in saline-alkali soil. Based on the discovery, the inventor prepares the three bacteria into the composite microbial inoculum by inoculating the three bacteria into MRS culture solution for culture and fermentation. Therefore, the invention provides an application of the complex microbial inoculum, namely: the lactobacillus composite microbial inoculum is applied to improving the peanut quality of saline-alkali soil; the lactobacillus composite microbial inoculum is applied to improving the yield and the characters of the peanuts in the saline-alkali soil.
In addition, the lactobacillus composite microbial inoculum related to the invention can be prepared by adopting the following method:
(1) respectively inoculating the Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in an MRS culture solution according to the inoculation amount of 4-10%, culturing at the constant temperature of 35-37 ℃ for 12-24 hours, centrifuging and washing, adding a mixed solution containing 8-12% of sterilized and defatted milk, 0.5-0.6% of glucose and 0.5-0.8% of yeast extract into each thallus precipitate, and adjusting the bacterial count to be more than 2.0 multiplied by 109cfu/mL, mixing uniformly and pouring and culturing respectively;
(2) respectively inoculating Lactobacillus bifidus (Lactobacillus animalis subsp.Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang strain cultures obtained in the step (1) into an MRS culture solution, culturing at the constant temperature of 35-37 ℃ for 18-24 hours, then respectively inoculating into a mixed solution containing 8-12% of sterilized skimmed milk, 0.5-0.6% of glucose and 0.5-0.8% of yeast extract, and culturing at the temperature of 35-37 ℃ for 18-24 hours;
(3) and (3) mixing the bacterial liquids obtained in the step (2) according to the ratio of Lactobacillus plantarum (P-8: lactobacillus casei (Lactobacillus casei) Zhang: lactobacillus plantarum (Lactobacillus plantarum) CCFM8661 is 1: (2-3): (2-3), and adding a protective agent into the mixed bacterial liquid, wherein the protective agent comprises the following components: 90-120g/L of skimmed milk powder, 25-35mL/L of glycerol, 90-120g/L of maltodextrin, 120-180g/L of lacca and 8-13g/L L-sodium glutamate, and the number of viable bacteria is adjusted to be more than 2 x 109cfu/mL, pH 5-6.
The lactobacillus composite bacterial agent can be applied to the peanut planting process after being prepared into bacterial liquid, and the using amount and the method are as follows: the first application is carried out, the use amount is 2 liters/mu (the total viable bacteria content is more than 2 multiplied by 10) in the peanut sowing period9cfu/mL), if a manual planting mode is adopted, after ditching, clear water is diluted, and an agricultural sprayer is adopted to spray the diluted clear water into the ditch; if the peanut sowing and film mulching all-in-one machine is used, the clean water of the bacterial liquid can be directly diluted and then the diluted bacterial liquid is placed in a pesticide barrel of the all-in-one machine for use, no matter what application method is adopted, the bacterial liquid and the pesticide cannot be used together, and the use time of the bacterial liquid and the pesticide is required to be separated by more than 2 days; the second application is carried out, in the peanut pod stage, the dosage is 2 liters/mu (the total viable count content is more than 2 multiplied by 10)9cfu/mL), spraying or irrigating the diluted solution on the root of the peanut, and carrying out drip irrigation in a drip irrigation mode under a film in a conditional area, wherein the soil is deeply wetted by the irrigation water by 8-10 cm.
Compared with the prior art, the active bacteria in the Lactobacillus composite microbial inoculum provided by the invention consist of Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang, and the Lactobacillus composite microbial inoculum has obvious influence on the growth and metabolism of peanuts in saline-alkali lands, and particularly can improve the quality and yield of the peanuts in the saline-alkali lands, so that a relatively ideal bacterial fertilizer raw material is provided for improving the quality and yield of the peanuts in the saline-alkali lands.
Detailed Description
The foregoing aspects of the present invention are described in further detail below by way of examples, but it should not be construed that the scope of the subject matter of the present invention is limited to the following examples, and that all the technologies realized based on the above aspects of the present invention are within the scope of the present invention. In addition, the experimental methods in the following examples are all conventional methods unless otherwise specified.
Example 1: preparation of composite lactic acid bacteria agent
(1) Respectively inoculating Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in 6% inoculum size in MRS culture solution, culturing at 35-37 deg.C for 18 hr, centrifuging, washing, adding 10% sterilized defatted milk, 0.6% glucose and 0.8% yeast extract into the thallus precipitate, and regulating the bacteria number to be greater than 2.0 × 109cfu/mL, mixed well and poured to culture respectively.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0g/L L-sodium glutamate.
(3) Respectively inoculating Lactobacillus bifidus (Lactobacillus animalis subsp.Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang strain cultures obtained in the step (1) into an MRS culture solution, culturing for 18 hours at the constant temperature of 35-37 ℃, inoculating into a mixed solution of 10% of sterilized skimmed milk, 0.6% of glucose and 0.8% of yeast extract, and culturing for 20 hours at the temperature of 35-37 ℃.
(4) Uniformly mixing the bacterial liquids obtained in the step (3) according to the proportion of Lactobacillus bifidus (Lactobacillus animalis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang of 2:2:1, adding the protective agent prepared in the step (2) into the mixed bacterial liquid, and adjusting the viable count to be more than 2 x 109cfu/mL, pH 5-6.
Example 2: preparation of composite lactic acid bacteria agent
(1) Respectively inoculating Lactobacillus plantarum P-8 and Lactobacillus casei Zhang in 6% inoculum size in MRS culture solution, culturing at constant temperature of 35-37 deg.C for 18 hr, centrifuging, washing, adding 10% sterilized defatted milk, 0.6% glucose and 0.8% yeast extract, and regulating bacteria number to be greater than 2.0 × 109cfu/mL, mixed well and poured to culture respectively.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0 g/LL-sodium glutamate.
(3) Respectively inoculating the Lactobacillus plantarum P-8 and Lactobacillus casei Zhang strain cultures obtained in the step (1) into an MRS culture solution, culturing for 18 hours at a constant temperature of 35-37 ℃, then inoculating into a mixed solution of 10% of sterilized defatted milk, 0.6% of glucose and 0.8% of yeast extract, and culturing for 20 hours at a temperature of 35-37 ℃.
(4) And (3) mixing the bacterial liquids obtained in the step (3) according to the ratio of Lactobacillus plantarum (P-8: lactobacillus casei (Lactobacillus casei) Zhang is 1: 1, adding the protective agent prepared in the step (2) into the mixed bacterial liquid, and adjusting the viable count to be more than 2 multiplied by 109cfu/mL, pH 5-6.
Example 3: preparation of bifidobacterium bacterial agent
(1) Inoculating Lactobacillus bifidus (Bifidobacterium animalis subsp. Lactis) V9 into MRS culture solution at 6%, culturing at constant temperature of 35-37 deg.C for 18 hr, centrifuging, washing, adding 10% sterilized defatted milk, 0.6% glucose and 0.8% yeast extract into thallus precipitate, and regulating the number of the thallus to be more than 2.0 × 109cfu/mL, mixed well and then poured for culture.
(2) The protective agent is prepared by using sterile water and comprises the following components: l00g/L skimmed milk powder, 30mL/L glycerol, L00g/L maltodextrin, 150g/L trehalose and L0 g/LL-sodium glutamate.
(3) Respectively inoculating the lactobacillus bifidus (Bifidobacterium animalis subsp. Lactis) V9 strain culture obtained in the step (1) into an MRS culture solution, culturing at constant temperature of 35-37 ℃ for 18 hours, inoculating into a mixed solution of 10% of sterilized defatted milk, 0.6% of glucose and 0.8% of yeast extract, and culturing at 35-37 ℃ for 20 hours.
(4) Adding the protective agent prepared in the step (2) into the culture solution in the step (3), and adjusting the viable count to be more than 2 multiplied by 109cfu/mL, pH 5-6.
Example 4: experiment of various microbial inoculum in saline-alkali soil peanut planting
The experimental site: rijin county Rizhen Luo Mao Tuo village of Shandong Dongying City
Experiment time: 2016.05-2016.10
Experimental crops: peanut (variety is small blue and white 9)
Experiment soil: moderate saline-alkali soil (soil salt content 2.83g/kg, pH 8.65)
Experimental groups were as follows: the microbial inoculum prepared in example 2 was administered to group a of composite bacteria, the microbial inoculum prepared in example 3 was administered to group B of bifidobacterium, and the microbial inoculum prepared in example 1 was administered to group B of composite bacteria.
The usage amount and the method are as follows: the first application is carried out, the using amount is 2 liters/mu (the bacterial agents prepared in each embodiment are respectively prepared into bacterial solutions, and the total viable bacteria content of each bacterial solution is 3 multiplied by 109About cfu/mL), if a manual planting mode is adopted, after ditching, clear water is diluted, and an agricultural sprayer is adopted to spray the diluted clear water into the ditch; if the peanut sowing and film mulching all-in-one machine is used, the clean water of the bacterial liquid can be directly diluted and then the diluted bacterial liquid is placed in a pesticide barrel of the all-in-one machine for use, no matter what application method is adopted, the bacterial liquid and the pesticide cannot be used together, and the use time of the bacterial liquid and the pesticide is required to be separated by more than 2 days; the second application, in the peanut pod stage, the dosage is 2 liters/mu (the bacterial agents prepared in each example are respectively prepared into bacterial liquid, and the total viable bacteria content in each bacterial liquid is 3 multiplied by 109cfu/mL), spraying or irrigating the diluted solution on the root of the peanut, and carrying out drip irrigation in a drip irrigation mode under a film in a conditioned area, wherein the soil is deeply wetted by the irrigation water by 8-10 cm.
Planting management: in each group of 1 mu test field, peanuts are planted in a film mulching and ridging mode, the ridge distance is 90cm, 2 rows are arranged on each ridge, the small row distance on each ridge is 35cm, the hole distance is 11cm (1 grain in each hole), and 13474 holes (plants) are formed in each mu. The special fertilizer for peanuts is applied to each mu, the nitrogen, phosphorus and potassium content is 45% (10: 18: 17), 50 kilograms are applied to each mu, and no additional fertilizer is applied at the later stage.
The experimental results are as follows:
1. influence on relevant indexes of peanut stems
After the composite lactobacillus inoculant is applied to peanuts in a saline-alkali land, plants are dwarf and strong, the nutrient consumption of ineffective growth on the overground part is reduced, the nutrient utilization of the plants is optimized, and the stress resistance of the plants is improved. Compared with the blank control group, the effective branch number of the peanuts in the compound lactobacillus B group is improved by 9.32%, the main stem thickness of the peanuts is improved by 5.98%, the branch thickness of the first pair is improved by 4.77%, the fresh weight of the stems is improved by 16.23%, and the dry weight is improved by 11.77% (table 1). Through statistical comparison, in the three aspects of the effective branch number, the fresh stem weight and the stem weight, the group B has significant difference (P is less than 0.05) compared with the group A and the group B of the lactobacillus bifidus, which shows that the lactobacillus bifidus V9, the lactobacillus plantarum P-8 and the lactobacillus casei Zhang can synergistically enhance the stress resistance of saline-alkali soil plants and improve the utilization rate of peanuts to nutrients.
TABLE 1 comparison of peanut Stem-related indices for various treatments
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus bifidus V9+ Lactobacillus plantarum P-8+ Lactobacillus casei Zhang. Compared with the blank control group, the composition of the composition,▲p is less than 0.05; the comparison of group B with group A,★p is less than 0.05; group B was compared with the group of Lactobacillus bifidus,■P＜0.05。
2. influence on related indexes of peanut leaves
Compared with the blank control group, the area of the peanut leaves of the compound lactobacillus B group is increased by 8.50%, the fresh weight of the leaves is increased by 13.33%, and the dry weight of the leaves is increased by 19.60% (table 2). Through statistical comparison, the group B has significant difference (P is less than 0.05) compared with the group A and the group B of lactobacillus bifidus in terms of fresh weight and dry weight of leaves, which shows that the lactobacillus bifidus V9, the lactobacillus plantarum P-8 and the lactobacillus casei Zhang can synergistically improve the number of leaves and the area of the leaves of peanut in saline-alkali land and is beneficial to improving the photosynthesis of the peanut.
TABLE 2 comparison of peanut leaf-related indices under each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus bifidus V9+ Lactobacillus plantarum P-8+ Lactobacillus casei Zhang. Compared with the blank control group, the composition of the composition,▲p is less than 0.05; the comparison of group B with group A,★p is less than 0.05; group B was compared with the group of Lactobacillus bifidus,■P＜0.05。
3. influence on indexes related to peanut needles
After the compound lactobacillus inoculant is applied to peanuts in a saline-alkali land, the quantity of fruit needles and the weight of an ovary stalk of the peanuts are obviously improved. Compared with the blank control group, the number of the peanut-planted fruit needles in the compound lactobacillus B group is increased by 29.04%, the fresh weight of the ovary stalk is increased by 76.05%, and the dry weight of the ovary stalk is increased by 42.79% (table 3). Through statistical comparison, the group B has significant difference (P is less than 0.05) compared with the group A and the group B of the lactobacillus bifidus in the three aspects of the number of the inserted fruit needles, the fresh weight and the dry weight of the ovary stalks, which shows that the lactobacillus bifidus V9, the lactobacillus plantarum P-8 and the lactobacillus casei Zhang can synergistically improve the number of the fruit needles and the weight of the ovary stalks of the peanuts.
TABLE 3 peanut pin correlation index comparison under each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus bifidus V9+ Lactobacillus plantarum P-8+ Lactobacillus casei Zhang. Compared with the blank control group, the composition of the composition,▲p is less than 0.05; the comparison of group B with group A,★p is less than 0.05; group B was compared with the group of Lactobacillus bifidus,■P＜0.05。
4. influence on the index of flower production
After the compound lactobacillus inoculant is applied to peanuts in saline-alkali lands, indexes such as the number and the weight of peanut pods are improved, and the yield of the peanuts is improved. Compared with the blank control group, the volume of the peanut pods is increased by 14.04%, the fresh weight of the peanut pods is increased by 13.13%, the dry weight of the peanut pods is increased by 6.98%, the total number of the peanut pods is increased by 30.64%, the plumpness of the peanut pods is increased by 16.0%, and the yield is increased by 7.86% in the compound lactobacillus B group (Table 4). Through statistical comparison, compared with the group A and the group B, the group B has significant differences (P is less than 0.05) in the aspects of pod volume, pod fresh weight, total fruit number and plump fruit number, which shows that the lactobacillus bifidus V9, the lactobacillus plantarum P-8 and the lactobacillus casei Zhang can synergistically improve indexes such as peanut pod number and weight and the like, and the yield of peanuts is improved.
TABLE 4 peanut yield-related index comparisons under various treatments
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus bifidus V9+ Lactobacillus plantarum P-8+ Lactobacillus casei Zhang. Compared with the blank control group, the composition of the composition,▲p is less than 0.05; the comparison of group B with group A,★p is less than 0.05; group B was compared with the group of Lactobacillus bifidus,■P＜0.05。
5. influence of lactobacillus application on peanut quality traits in saline-alkali soil
Compared with the blank control group, the protein content of the compound lactobacillus B group is increased by 4.0 percent, and the fat content is increased by 0.7 percent (table 5). Through statistical comparison, the group B has significant difference (P is less than 0.05) compared with the group A and the group B of the lactobacillus bifidus in terms of protein content, which shows that the lactobacillus bifidus V9, the lactobacillus plantarum P-8 and the lactobacillus casei Zhang can synergistically improve the protein content in peanut kernels, thereby improving the quality of peanuts in saline-alkali lands.
TABLE 5 comparison of peanut quality-related indicators for each treatment
Note: group A is Lactobacillus plantarum P-8+ Lactobacillus casei Zhang, group B is Lactobacillus bifidus V9+ Lactobacillus plantarum P-8+ Lactobacillus casei Zhang. Compared with the blank control group, the composition of the composition,▲p is less than 0.05; the comparison of group B with group A,★p is less than 0.05; group B was compared with the group of Lactobacillus bifidus,■P＜0.05。
conclusion and discussion:
after the compound lactobacillus microbial inoculum is applied to peanuts in saline-alkali lands, the germination rate of the peanuts is improved, the growth of invalid stems on the lands is reduced, the area and the quantity of leaves are increased, the quantity and the weight of pods in the underground parts are increased, and the economic characters and the yield of the peanuts are improved. From the quality perspective, the compound lactobacillus inoculant provided by the invention improves the protein content with higher nutritive value in peanuts, has no significant influence on the fat content, and has a certain positive effect on improving the quality of peanuts in saline-alkali lands.
Claims (6)
1. The application of a Lactobacillus composite bacterial agent in increasing the protein content of peanut kernels in saline-alkali lands is characterized in that the Lactobacillus composite bacterial agent is prepared from Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang according to the weight ratio of 1: (2-3): (2-3) the ratio of viable bacteria number.
2. The application of the Lactobacillus composite inoculant in improving the yield traits of peanuts in saline-alkali soil, wherein the yield traits are pod volume, pod fresh weight, total fruit number and full fruit number, and the Lactobacillus composite inoculant is prepared by Lactobacillus bifidus (Lactobacillus plantarum subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang according to the ratio of 1: (2-3): (2-3) the ratio of viable bacteria number.
3. Peanut planting method for increasing protein content in peanut kernels in saline-alkali land and improving yield traitsThe method is characterized by comprising the step of applying a bacterium solution prepared from Lactobacillus bifidus (Lactobacillus animalis subsp. Lactis) V9, Lactobacillus plantarum P-8 and Lactobacillus casei Zhang according to the ratio of 1: (2-3): (2-3) the number ratio of viable bacteria, the amount of the viable bacteria is 2-3 liters per mu when the viable bacteria is applied to the peanut seeding period for the first time, and the total number of the viable bacteria is more than 2 multiplied by 109cfu/mL; applying to peanut in the pod stage for the second time, with the dosage of 2-3L/mu, and the total number of viable bacteria greater than 2 × 109cfu/mL, diluted by water, and sprayed or irrigated or drip-irrigated on the root of the peanut.
4. The peanut planting method for improving the protein content and the yield traits of peanut kernels in the saline-alkali soil according to claim 3, wherein if an artificial planting mode is adopted, the bacterial liquid is diluted by adding clear water after ditching, and is sprayed into the ditches by using an agricultural sprayer.
5. The peanut planting method for improving the protein content and the yield traits of peanut kernels in the saline-alkali soil according to claim 3, wherein if a peanut sowing and film covering integrated machine is adopted, the bacterial liquid is directly diluted with clear water and then placed in a pesticide barrel of the integrated machine for use.
6. The method for planting peanuts having increased protein content and improved yield and characteristics in saline-alkali soil peanut kernels according to any one of claims 3 to 5, wherein the bacterial liquid and the pesticide cannot be used together, and the time interval between the use of the bacterial liquid and the use of the pesticide is more than 2 days.
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| Growth responses of lactic acid bacteria to leguminous seed extracts.;Hoi-seon lee and young-joon ahn;《Agricultural chemistry and biotechnology》;19971231;第40卷(第2期);167-171页 * |
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