CN106282052A - A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof - Google Patents
A kind of compound lactobacillus preparation improveing coastal saline soil and application thereof Download PDFInfo
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Abstract
The invention discloses a kind of compound lactobacillus microbial inoculum improveing coastal saline soil and application thereof, this microbial inoculum is made up of Lactobacillus plantarum (Lactobacillus plantarum) P 8 and lactobacillus casei (Lactobacillus casei) Zhang.Both bacterium have significantly impact to the growth metabolism of plant on saline-alkali soil, can work in coordination with the raising germination percentage of plant, regularity, the uniformity and plant strain growth ability.
Description
Technical field
The present invention relates to a kind of microbial bacterial agent, particularly relate to one and can improve coastal saline soil, significantly improve and plant
The compound lactobacillus preparation of the anti-Saline Alkali Stress of thing, belongs to microbial technology field.
Background technology
The Huanghe delta is China or even the fastest Coastal Ecosystem of world's epeirogenetic, new since within 1855, changing its course
Increasing land area more than 6000 square kilometre, newborn soil provides wide standby cultivated land resource, is ensureing China's land resource
Safety and ecosystem service function there is considerable effect.But in the last few years, the blindness cultivation of the mankind utilized, and caused
In district, Main Agricultural utilizes new alluvial plain, soil and the Saline of improvement, and the accumulation of salt in the surface soil is degenerated the most with surprising rapidity, gas in addition
Waiting change impact, the self-purification capacity of ecological environment, resistivity and recovery capability are deteriorated, and the new alluvial plain that there are about 5% every year is become
Heavy saline and saline-alkali wasteland, ecosystem function is degenerated serious, and land and water resources environmental quality declines, and it recovers and rebuilds to become
For current study hotspot.
Blended subsoil water shallow, salty by Seawater immersion side to be affected, beach saline land has that salinity is high, easily hardens, structure is poor,
The characteristics such as the content of organic matter is low, storing water and preserving fertility ability is weak, determine its agriculture during the initial stage of enclosing and cultivating to desalination to normal soil
Industry utilizes difficulty very big, and agricultural use mode makes the salinization of soil have repeatability the most improperly, explores the most efficient
By good for coastal saline-alkali land reform be problem demanding prompt solution for planting technique soil.
Currently, coastal saline-alkali soil improving technology mainly uses such as: spray water discharge method, by soil moved in improve the original improvement, improving straw mulching
Deng physical method or the chemical method that utilizes some acid salts substances improvement, but these methods exist, and engineering amount is big, consumption
The defects such as money height, cycle length, such as " irrigation and drainage by well " method and " forced-ventilated fills by force " method etc., it needs the water conservancy projects investment set up huge,
Maintenance cost is high；Soil moved in improve the original improvement not only needs to transport substantial amounts of soil moved in improve the original, and causes huge often to the farmland in soil moved in improve the original source place
Big destruction；Chemical method is owing to will constantly suffer erosion before and after administering directly facing sea, and therefore effect is very limited,
It is difficult to promote.
Biological modification technology compared with above-mentioned measure, have with low cost, material source is extensive, easy to use, promote make
The advantages such as thing root system, acceleration crumb structure formation, process green safety, environmental friendliness have no side effect.But, current biology changes
Good technology is only for the improvement in the salt-soda soil such as northwest, the Northeast, has no applicable Yellow River Delta Coastal beach saline-alkali ground
Correlation technique measure.
Summary of the invention
Develop difficult problem for Huanghe delta standby cultivated land resource, object of the present invention is to provide one
The composite bacteria agent capable of improvement coastal saline soil and application thereof.
In order to realize the purpose of the present invention, inventor, by lot of experiments research unremitting effort, is finally obtained as follows
Technical scheme:
A kind of compound lactobacillus microbial inoculum improveing coastal saline soil, this microbial inoculum is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang composition.
It should be noted that Lactobacillus plantarum of the present invention (Lactobacillus plantarum) P-8, point
From in Inner Mongol traditional natural fermentation clabber, there is the anti-gastrointestinal tract Digestive system tolerance of excellence, it is possible to survive in dynamic
In thing intestinal；This bacterial strain has been preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center, and address is China
Yard 1, BeiChen xi Road, Chaoyang District, Beijing City 3, the preservation time is on 06 28th, 2012, and preserving number is CGMCC No.6312.
It addition, lactobacillus casei of the present invention (Lactobacillus casei) Zhang, it is the resistance to of separation from koumiss
The probiotics strain of sour and resistance to bile acid；This bacterial strain is preserved in Chinese microorganism strain preservation management on April 21st, 2006 and entrusts
Member's meeting General Microbiological Culture preservation center, preserving number CGMCC No.1697.
Preferably, composite bacteria agent capable as above, this microbial inoculum is by Lactobacillus plantarum (Lactobacillus plantarum
P-8) and lactobacillus casei (Lactobacillus casei) Zhang presses 1:(0.5～3) number of viable than composition.
The composite bacteria agent capable that the present invention relates to can be adopted and prepare with the following method:
(1) by described lactobacillus casei bacterial strain (Lactobacillus casei) Zhang and lactobacillus plantarum strain
(Lactobacillus plantarum) P-8 is inoculated in MRS culture fluid, cultivation 12-24 hour under the conditions of 37 DEG C, by centrifugation
After washing, bacterial sediment adds 8%-12% sterilized non-fat Lac Bovis seu Bubali liquid, and to adjust its bacterium number be 2.0 × 1010Cfu/mL, mixed
Cultivation is poured into after even；
(2) strain culture that step (1) obtains is inoculated in MRS culture fluid, under the conditions of 37 DEG C, cultivates 12-24 little
Time, inoculate the sterilized non-fat Lac Bovis seu Bubali liquid of 8%-12%, cultivate 6-10 hour under the conditions of 37 DEG C, adjust its bacterium number be 3 ×
109Cfu/mL, pH value is 4.4～4.6.
The present inventor has been surprisingly found that, containing Lactobacillus plantarum (Lactobacillus when screening Salt And Alkali Tolerance bacterial strain
Plantarum) composite bacteria liquid of P-8 and lactobacillus casei (Lactobacillus casei) Zhang has certain Salt And Alkali Tolerance to make
With, and the growth metabolism of plant on saline-alkali soil is had obvious facilitation.Finding based on this, the present inventor is by being inoculated in
Cultivation and fermentation in MRS culture fluid, has been prepared as composite bacteria agent capable by both bacterium.Therefore, the invention provides above-mentioned composite bacteria agent capable
Application, it may be assumed that
A kind of composite bacteria agent capable is in the application improved during plant anti-salt is coerced, and described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
The application in improving Genes For Plant Tolerance alkaline stress of a kind of composite bacteria agent capable, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
A kind of composite bacteria agent capable improves the application during plant anti-salt alkali resistant is coerced at the same time, and described composite bacteria agent capable is by plant breast
Bacillus (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang is by 1:
The number of viable of (0.5～3) is than composition.
The application in improvement coastal saline soil of a kind of composite bacteria agent capable, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
The application in preparation reclamation of salinep-alkali soil liquid of a kind of composite bacteria agent capable, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
It should be strongly noted that in the embodiment that the present invention records, above-mentioned composite bacteria agent capable sprays after being prepared as bacterium solution
Semen arachidis hypogaeae can be improved or Salt-resistant of Cotton alkali resistant is coerced, and promote germination, growth that it is good.
A kind of improving the implantation methods that Semen arachidis hypogaeae anti-salt alkali resistant is coerced it addition, present invention also offers, the method includes: broadcasting
Kind of front plot is ploughed deeply, in conjunction with per hectare of ploughing use carbamide 200～300 jin, Diammonium phosphate (DAP) 300～400 jin, potassium sulfate 100～
The suppression of 150 jin of ridgings, with every mu of sowing 10000～11000 caves, the 1-3 strain of every cave, after planting covering with plastic film, the later stage no longer carries out
Topdress and irrigate；After peanut seeding terminates, spraying total viable count is 1.0 × 109～6.0 × 109The infected water of cfu/mL,
The amount of spraying is 2-3 liter/mu；Spraying total viable count again in the peanut pin phase is 1.0 × 109～6.0 × 109Cfu/mL contains
Bacterium water, the amount of spraying is 2-3 liter/mu.
Compared with prior art, the composite bacteria agent capable that the present invention relates to is by Lactobacillus plantarum (Lactobacillus
Plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang composition, both is planted by bacterium on saline-alkali soil
The growth metabolism of thing has significantly impact, can work in coordination with the raising germination percentage of plant, regularity, the uniformity and plant strain growth energy
Power.
Detailed description of the invention
The foregoing of the present invention is described in further detail by form more by the following examples, but should this not managed
Solving and be only limitted to below example for the scope of the above-mentioned theme of the present invention, all technology realized based on foregoing of the present invention are equal
Belong to the scope of the present invention.It addition, the experimental technique in following embodiment, if no special instructions, it is conventional method.
Embodiment 1: Lactobacillus plantarum (Lactobacillus plantarum P-8) and lactobacillus casei
(Lactobacillus casei) Zhang is improving salt-soda soil Semen arachidis hypogaeae Seedling growing way, regularity and the test of the uniformity
Experiment place: Mao Tuo village, Ting Luo town, Dongying City Lijin County-moderate saline-alkali soil (Lactobacillus plantarum group, lactobacillus casei
Each 1 mu of group, compound bacteria group and blank group)
Cultivate administration: sow front plot and plough deeply, uses carbamide 200～300 jin, Diammonium phosphate (DAP) in conjunction with ploughing by per hectare
The standard ridging suppression of 300～400 jin, potassium sulfate 100～150 jin, with every mu of sowing 10000～11000 caves, every cave 2 strain, broadcasts
Covering with plastic film after Zhong.Later stage no longer topdresses and irrigates.
Experimental period: 2016.06.2-2016.8.4
Experiment crop: Semen arachidis hypogaeae
Usage amount and method: after peanut seeding terminates, experimental group sprays the microbial bacterial agent (bacterium that experimental group uses immediately
Total viable count content 3.0 × 10 in agent9Cfu/mL, in compound bacteria group, the quantity of two kinds of bacterium is than for 1:1) 2 liters/mu.At peanut
The pin phase sprays microbial bacterial agent again, and (in the microbial inoculum that experimental group uses, total viable count content is more than 109Cfu/mL, compound bacteria group
In the quantity of two kinds of bacterium than for 1:1) 3 liters/mu, other way to manages of matched group and experimental group are identical.
After peanut seeding 15 days, each plot that processes randomly selects the sample prescription of 52 respectively, measures germination percentage；The really pin phase
After applying fertilizer 15 days, each plot that processes randomly selects the sample prescription of 52 respectively, measures Semen arachidis hypogaeae plant height.
Table 1 respectively organizes field experiment Semen arachidis hypogaeae germination percentage and plant height compares
Compare with blank group,▲P ＜ 0.01；Compare with Lactobacillus plantarum group,P ＜ 0.01；With lactobacillus casei group
Relatively,■P ＜ 0.01.
From the test statistics result of table 1, saline-alkali soil uses compound lactobacillus preparation, it is possible to increase peanut seed
Germination percentage and the plant height of plant, all have pole significant difference (p < 0.01), relatively compared with blank group or single bacterium group
In blank group, lactobacillus casei group, Lactobacillus plantarum group, the germination percentage of Semen arachidis hypogaeae has been respectively increased 24.99%, 23.08%,
19.09%, plant height has been respectively increased 20.78%, 16.22%, 25.0%.
Embodiment 2: Lactobacillus plantarum (Lactobacillus plantarum P-8) and lactobacillus casei
(Lactobacillus casei) Zhang is on the impact test of cotton germination rate, Seedling length and root length under Salt Strees Condition
Experiment place: Qingdao Agricultural University's experimental teaching of agronomy room
Test is arranged: we find early-stage Study, and yellow coastal saline-alkali area, delta topsoil base cation is mainly:
Na+、HCO3-、Cl-、SO3 2-, in order to simulate this strand nature solonchak composition, we use sodium chloride, sodium sulfate and sodium bicarbonate
Three kinds of mixed salts, equal proportion (1:1:1) is mixed in the foundation soil after natural air drying, roguing of sieving, according to soil salinity characters classification
Standard, configures 4 salinity gradients as follows: conventional (0.6g/kg), light salt (1.1g/kg), middle salt (2.9g/kg), geavy salt (5.1g/
kg)。
The soil of different salinity gradients that will have configured, is laid in the culture dish of diameter 9cm, added in each culture dish
Soil amount is identical, each culture dish 20 seeds of sowing, planting seed as far as possible uniformly, make each seedling have abundance growing space and
Nutrient substance.
Experimental period: 2016.7.6-2016.7.13
Experiment crop: Cotton Gossypii
Usage amount and method: each salinity gradient divides into Lactobacillus plantarum group, lactobacillus casei group, compound bacteria group and blank
Matched group, by bacterium solution (total viable count content 5.0 × 109Cfu/mL, in compound bacteria group, the quantity of two kinds of bacterium is than for 1:1) with 1:
After the concentration dilution of 500, each culture dish adds 1ml diluent, and blank adds the distilled water of equivalent, and later every day claims
Weight method supplements sterile distilled water.
All culture dishs are put in the biochemical cultivation case of 25+1 DEG C and germinate.Seed is added up in the 7d of Seed germination
Germinative number, calculates germination percentage (germination percentage (%)=broadcast rear 7d chitting piece number/sowing seed number * 100%).Germination standard is embryo
Root reaches the 1/3 of seed length.The 7d of Seed germination terminates to germinate, and measures the Seedling length (height of seedling: hypocotyl base portion is to bud top of seedling
The length of end), main root length (main root is long: the length that hypocotyl base portion to main root is most advanced and sophisticated).
Cotton germination rate, height of seedling and root length under each salinity gradient of table 2 embodiment 2
Compare with blank group,▲P ＜ 0.01；Compare with Lactobacillus plantarum group,P ＜ 0.01；With lactobacillus casei group
Relatively,■P ＜ 0.01.
From table 2 result of the test, under non-saline-alkali environment, either compound bacteria group or single bacterium group, it compares blank right
All can limit germination percentage and the Seedling length (P ＜ 0.01) improving Cotton Gossypii according to group, and the Seedling significantly improving Cotton Gossypii is long.And compound bacteria group with
Lactobacillus plantarum group, lactobacillus casei group list bacterium group compare no significant difference (P ＞ 0.05), imply that at nonsaline-alkali soil on the ground,
Lactobacillus plantarum or lactobacillus casei all can limit germination and the growth promoting Cotton Gossypii.
It addition, use compound lactobacillus preparation on saline-alkali soil, it is possible to increase the germination percentage of cotton seeds, different Salt Strees Conditions
Under be all remarkably higher than blank group, Lactobacillus plantarum group or lactobacillus casei group (P ＜ 0.01), this imply that Lactobacillus plantarum
The germination percentage improving Cotton Gossypii can be worked in coordination with lactobacillus casei compound use.And along with the raising of soil salts density, compound bacteria
The growth-promoting ability of Radix Gossypii is strengthened by group, light, in, under severe salinity, compound bacteria group is respectively increased relative to blank group
12.03%, 16.73% and 155.86%.
Compound lactobacillus preparation is used, it is possible to increase the Seedling of cotton seeds is long on saline-alkali soil, notable under slight Salt Strees Condition
Higher than blank group, Lactobacillus plantarum group or lactobacillus casei group (P ＜ 0.01), this imply that Lactobacillus plantarum and cheese milk
The Seedling that bacillus compound use can work in coordination with raising Cotton Gossypii under low saline-alkali environment is long.Relative to comparison energy on slight and heavy salinized soil
The Seedling enough significantly improving cotton seeds is long, though it is long to improve Seedling on moderate solonchak, but and no significant difference (P between blank group
＞ 0.05).Gently, in, under severe salinity, it is respectively increased 42.31%, 10.21% and relative to blank group Seedling length
52.51%.
Compound lactobacillus preparation is used, by increasing capacitance it is possible to increase the root length of cotton seeds on saline-alkali soil, the most aobvious under different Salt Strees Conditions
Write higher than blank group, Lactobacillus plantarum group or lactobacillus casei group (P ＜ 0.01), this imply that Lactobacillus plantarum and cheese
Lactobacillus compound use can work in coordination with the root length improving Cotton Gossypii.And along with the raising of soil salts density, compound bacteria group is to Radix Gossypii
Growth-promoting ability the strongest, light, in, under severe salinity, be respectively increased 21.89%, 22.38% and relative to blank group
30.80%.
Claims (7)
1. composite bacteria agent capable is in the application improved during plant anti-salt is coerced, and described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
2. a composite bacteria agent capable application in improving Genes For Plant Tolerance alkaline stress, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
3. composite bacteria agent capable improves the application during plant anti-salt alkali resistant is coerced at the same time, and described composite bacteria agent capable is by plant breast bar
Bacterium (Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang presses 1:(0.5
～3) number of viable than composition.
4. according to the application described in any one of claim 1-5, it is characterised in that described plant is Semen arachidis hypogaeae or Cotton Gossypii.
5. a composite bacteria agent capable application in improvement coastal saline soil, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
6. a composite bacteria agent capable application in preparation reclamation of salinep-alkali soil liquid, described composite bacteria agent capable is by Lactobacillus plantarum
(Lactobacillus plantarum) P-8 and lactobacillus casei (Lactobacillus casei) Zhang press 1:(0.5～
3) number of viable is than composition.
7. one kind is improved the implantation methods that Semen arachidis hypogaeae anti-salt alkali resistant is coerced, it is characterised in that the method includes: plot is deep prior to seeding
Turn over, use carbamide 200～300 jin, Diammonium phosphate (DAP) 300～400 jin, potassium sulfate 100～150 jin of ridging towns in conjunction with per hectare of ploughing
Pressure, with every mu of sowing 10000～11000 caves, the 1-3 strain of every cave, after planting covering with plastic film, the later stage no longer topdresses and irrigates；
After peanut seeding terminates, spraying total viable count is 1.0 × 109～6.0 × 109The infected water of cfu/mL, the amount of spraying is 2-3
Liter/mu；Spraying total viable count again in the peanut pin phase is 1.0 × 109～6.0 × 109The infected water of cfu/mL, the amount of spraying
For 2-3 liter/mu.
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Cited By (3)
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| CN107129951A (en) * | 2017-06-19 | 2017-09-05 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate and its application |
| CN108410470A (en) * | 2018-03-05 | 2018-08-17 | 中国农业科学院农业环境与可持续发展研究所 | A kind of microbial soil conditioner and preparation method thereof for salt-soda soil |
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| CN107129952A (en) * | 2017-06-19 | 2017-09-05 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of lactic acid bacteria composite fungicide of raising salt-soda soil peanut yield character and its application |
| CN107129951A (en) * | 2017-06-19 | 2017-09-05 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | A kind of compound lactobacillus preparation for improving saline and alkaline tibet milkwort root micro-nutrient utilization rate and its application |
| CN107129952B (en) * | 2017-06-19 | 2020-05-05 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | Lactobacillus composite microbial agent for improving yield and characters of peanuts in saline-alkali soil and application thereof |
| CN107129951B (en) * | 2017-06-19 | 2020-07-07 | 青岛科拓恒通乳酸菌产业化开发研究院有限公司 | Compound lactobacillus preparation for improving utilization rate of trace nutrients of peanuts in saline-alkali soil and application thereof |
| CN108410470A (en) * | 2018-03-05 | 2018-08-17 | 中国农业科学院农业环境与可持续发展研究所 | A kind of microbial soil conditioner and preparation method thereof for salt-soda soil |
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| CN106282052B (en) | 2019-06-07 |
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