CN103299806B - Switchgrass cuttage vegetative propagation method - Google Patents
Switchgrass cuttage vegetative propagation method Download PDFInfo
- Publication number
- CN103299806B CN103299806B CN201310218910.2A CN201310218910A CN103299806B CN 103299806 B CN103299806 B CN 103299806B CN 201310218910 A CN201310218910 A CN 201310218910A CN 103299806 B CN103299806 B CN 103299806B
- Authority
- CN
- China
- Prior art keywords
- switchgrass
- cutting
- cuttage
- bud
- reproduction method
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a switchgrass cuttage vegetative propagation method which comprises the following steps: when the switchgrass grows to period with 4-6 knobs, removing the top and leaving 3-4 knobs below the top so as to break the apical dominance and prompt the growth of axillary buds on the knobs below; when lateral buds of 15-20 cm long grow from the axillary buds, cutting the lateral buds together with the parts which are 5 cm long above and below the knobs, so as to be the cuttings; trimming the cuttings and carrying out TDZ (Thidiazuron) rooting powder treatment, and cuttaging the cuttings into a culture medium to carry out rooting culture; and transplanting to a greenhouse or a land after the rooting is accomplished. According to the switchgrass cuttage vegetative propagation method disclosed by the invention, the vegetative propagation is tried on perennial switchgrass of the grass family by using the cuttaging method, and unexpected and good breeding effect is achieved; and the method has the characteristics of being simple and feasible, high in survival rate, unlikely to have variation and the like, and is particularly applicable to providing materials for switchgrass biotechnology study.
Description
Technical field
The invention belongs to switchgrass propagation technique field, relate to a kind of switchgrass cuttage asexual reproduction method.
Background technology
Switchgrass (English name Switchgrass, latin name Panicum virgatum Linn) be grass family herbaceos perennial, the former north America region of being born in, there is the features such as cultivation is simple, strong stress resistance, wide adaptability, since early 1990s, China's researcher is progressively introduced a fine variety the areas such as semiarid loess hill-valley area and Ansai, Shaanxi.Switchgrass belongs to C
4class plant, there is very high year biomass yield, mainly be used as at first forage crop and gardens crop, the eighties in 20th century, (the US Department Of Energy of american energy department, DOE) be chosen to be pattern energy-source plant, after this, various countries have all strengthened the research to switchgrass.
At present both at home and abroad the research of switchgrass is mainly concentrated on the fields such as agricultural science, environmental science and bioenergy conversion, and at the research proportion of switchgrass biological technical field seldom.As a kind of pattern energy-source plant, switchgrass has a lot of merits, as tall and big in drought-enduring, impoverishment tolerant, disease and insect resistance, plant, tiller manyly etc., has good biotechnology research prospect.Yet switchgrass is a kind of cross-pollinatd plant, there is very strong certainly colored incompatibility, therefore, the offspring that sexual propagation obtains often has different genotype, and this is unfavorable for switchgrass biotechnology research.
Vegetative propagation is the process of whole plant that directly obtained by the organ of plant, and owing to not relating to Gene Isolation and genetic recombination in this process, so the offspring plant of gained has identical genotype.At present, switchgrass asexual reproduction method has by callus plantlet with by the induced bundle Fast-propagation of sprouting.The tradition cycle is long, and in Induction Process, has certain probability to produce variation, so take callus induction regrowth method and provide material improper as biotechnology research.
Cuttage is a kind of comparatively ripe vegetative propagation technique, but is usually used in dicotyledon, also only has Liliaceae Asparagus and Commelianaceae Activities of Some Plants to utilize cottage propagation mode, and seldom find application in monocotyledon in grass family.
Summary of the invention
The problem that the present invention solves is to provide a kind of switchgrass cuttage asexual reproduction method, and the method is a kind of easy switchgrass propagation method, is conducive to obtain the progeny material that genotype is consistent.
The present invention is achieved through the following technical solutions:
A switchgrass cuttage asexual reproduction method, comprises the following steps:
1) after switchgrass grows 4～6 joints, remove and tiller top and retain 3～4 joints in below, axillalry bud is germinated.The top of tillering was cut after 20～30 days, and axillary bud growth becomes the long lateral bud of 15～20cm;
2) lateral bud is saved and saved each 3～5cm up and down together with its place and cut from switchgrass plant, as cutting; Peel off the blade that wraps up lateral bud on cutting base portion maternal plant, and cutting lower end is whittled into wedge shape;
3) the cutting bottom after pruning is immersed in rooting powder solution more than 12h, then cutting is inserted in the culturing pot that loose permeable culture matrix is housed, culturing pot is placed in to water, make culture matrix from bottom, start immersion, until culture matrix water suction is saturated; Then culturing pot is placed in constant temperature greenhouse and is cultivated, treat that new root grows to 10～20cm long;
4) cutting of having taken root in culturing pot is taken out, transplant in greenhouse.
The described removal top of tillering is in 7～August, treats that switchgrass grows 4～6 internodes that extend completely, and the individual plant of the switchgrass of selected robust growth is removed and tillered top and retain 3～4 joints in below.
Described after lateral bud is cut as cutting from switchgrass, the axillalry bud that the below of lateral bud place joint is close on joint germinates, and after this axillary bud growth to 15～20cm is long, carries out second take turns cuttage using it as cutting.
Described root-inducing powder is TDZ root-inducing powder (TDZ, Thidiazuron, the phenyl thiadiazolyl group urea of 500～1000 times of dilutions; Sai Gao bio tech ltd, Shaanxi, No. 9, the Dong Yi of Xi'an new and high-tech development zone road, postcode: 710021), carry out cuttage after rooting powder solution soaks 24h.
Described culture matrix is that 1～2 part of 1～2 part of cultivating soil, 4～6 parts of leaf moulds and river sand is fully mixed and obtained.
It is to cultivate 6～8 days in 20～25 ℃, the constant temperature greenhouse of 14h illumination every day that described culturing pot is placed to temperature.
The kind of described switchgrass is that the U.S. introduces cultivar Alamo.
Compared with prior art, the present invention has following useful technique effect:
Switchgrass cuttage asexual reproduction method provided by the invention, attempts adopting cottage method to carry out vegetative propagation to the perennial switchgrass of grass family, has obtained unexpected reproductive effect; And in reproductive process, do not relate to tissue culture procedures, compare with traditional callus induction differentiation and regeneration plant and inducing clumping bud Fast-propagation have simple, survival rate is high, and the feature such as be difficult for morphing, is very suitable for providing material for switchgrass biotechnology research.
Switchgrass cuttage asexual reproduction method provided by the invention, incubation is simple:
No matter be that callus induction regeneration plant Fast-propagation or inducing clumping bud Fast-propagation all relate to tissue culture procedures, need to explant carry out disinfection and be inoculated on dissimilar medium induce, regenerate, take root, the series of steps such as hardening, transplanting.Tissue is cultivated each process and is all required strictly, and often relate to the FAQs such as pollution, albefaction, somaclonal variation in the process of cultivating, and the present invention adopts cottage propagation, the loaded down with trivial details steps such as the sterilization in tissue culture procedures, induction have been removed from, and each step is compared all very simple with tissue cultivation, the cutting after having taken root can be directly used in transplanting.
Switchgrass cuttage asexual reproduction method provided by the invention, equipment is simple, survival rate is high:
In this switchgrass asexual reproduction method implementation process, do not relate to complicated experimental facilities, under greenhouse experiment, can carry out, required is only cuttage common tool, material and commercially available root-inducing powder etc., and condition is controlled survival rate very high (cuttage root-taking survival rate is 100% in specific experiment) in good situation.
Switchgrass cuttage asexual reproduction method provided by the invention, can repeatedly carry out for 1 year:
7～August, switchgrass grows 4～6 internodes that extend completely, cutting switchgrass removal apical dominance can carry out at twice, gather in for the first time switchgrass is left to 3～4 joints, now the axillalry bud on left top joint germinates, and the axillalry bud of below is still in suppressed state, about 30 days, it is long that uppermost axillalry bud can grow into 15～20cm, can cut as cutting, after cutting is cut, the axillalry bud that below is close on joint germinates, the lateral bud growing can be done second and take turns cuttage, so, can further improve and expands numerous efficiency.
Switchgrass cuttage asexual reproduction method provided by the invention, can expand rapidly a certain genotype plant colony:
Because whole process does not relate to chromosomal separation and restructuring, therefore, resulting offspring's genotype and parental plant genotype are in full accord.Meanwhile, the method can also be for the quick expanding propagation of the transfer-gen plant that obtains by animal nutrition.
Switchgrass cuttage asexual reproduction method provided by the invention, is not used complicated experimental instrument and equipment, and medicine, instrument and material are all very simple, compares with original method, greatly reduces production cost.
Accompanying drawing explanation
Fig. 1 is cottage propagation schematic flow sheet of the present invention;
Fig. 2 is the cutting schematic diagram after pruning;
Fig. 3 is the switchgrass schematic diagram after taking root.
Embodiment
Switchgrass cuttage asexual reproduction method provided by the invention, pilot production is carried out vegetative propagation with cottage method to the perennial switchgrass of grass family, has obtained good reproductive effect, has simplely, and survival rate is high, and the feature such as be difficult for morphing.Below in conjunction with specific embodiment, the present invention is described in further detail, and the explanation of the invention is not limited.
Referring to Fig. 1, a kind of switchgrass cuttage asexual reproduction method, comprises that axillary bud development induction, cutting are drawn materials, clip cutting and processing, rooting of cuttings cultivation, intermediate house etc., and step is as follows:
1) after switchgrass grows 4～6 joints, remove and tiller top and retain 3～4 joints in below, axillalry bud is germinated.Wait the top of tillering, cut after 20～30 days, axillary bud growth becomes the long lateral bud of 15～20cm;
2) by lateral bud together with its place joint and up and down each 3～5cm from switchgrass plant, cut, as cutting; Peel off the blade that wraps up lateral bud on cutting base portion maternal plant, and cutting lower end is whittled into wedge shape;
Concrete, the cutting after pruning is as shown in Figure 2;
3) the cutting bottom after pruning is immersed in rooting powder solution at least 12h, then cutting is inserted in the culturing pot that loose permeable culture matrix is housed, culturing pot is placed in to water, make culture matrix from bottom, start immersion, until culture matrix water suction is saturated; Then culturing pot is placed in constant temperature greenhouse and is cultivated, treat that new root grows to 10～20cm long;
Switchgrass after concrete taking root as shown in Figure 3;
4) cutting of having taken root in culturing pot is taken out, transplant in greenhouse.Under greenhouse constant temperature, cuttage seeding survival rate can reach 100%, can grow then 5～10 and tiller; Second Year moves to outdoor, can obtain the plant of normal growth, and the Second Year seedling of growing way and seminal propagation is suitable.
Further, concrete operation comprises the following steps:
(1) remove apical dominance, induction axillary bud development:
A. for to guarantee that switchgrass has high vigor and guarantees sufficient energy supply, sampling time of explant is defined as switchgrass and grows 4～6 joints and be advisable.
B. remove the top of tillering, retain 3～4 joints in below, break switchgrass apical dominance, axillalry bud germinates.
(2) culture matrix is prepared:
Culture matrix adopts loose permeable material, is beneficial to the growth of cutting root.The flower culturing soil of matrix used for buying in this method, composition is 2 parts of cultivating soils (garden mould), 6 parts of leaf moulds, 2 parts of river sands.
(3) draw materials:
A. when switchgrass top was cut after 20～30 days, top axillalry bud can grow into the long lateral bud of 15～20cm.
B. by lateral bud its together with its place joint up and down each 3～5cm from plant, cut.
(4) cutting is processed:
A. peel off the blade that is wrapped in lateral bud on cutting base portion maternal plant, and cutting lower end is whittled into wedge shape, so that cuttage.
B. the cutting bottom after pruning is immersed in to mass concentration and is 24h in 0.1% TDZ rooting powder solution.
(5) cuttage is cultivated:
A. the cutting after processing is inserted and is equipped with in the culturing pot of culture matrix, culturing pot is placed in basin, and adds water in basin, makes culture matrix from bottom, start immersion, until it is saturated to absorb water.
B. culturing pot is placed in 20 ℃ of constant temperature greenhouses and cultivates, about one week, it is long that new root can grow to 10～20cm.
(6) transplant:
The cutting of having taken root in culturing pot is taken out, transplant in greenhouse, so far, cutting can normal growth.
This kind of switchgrass cultivar Alamo(of take is below introduced cultivation by the U.S.) individual plant carries out fast-propagation and is described in detail as specific embodiment.
Embodiment 1:
The cuttage asexual reproduction method of switchgrass cultivar Alamo, comprises following operation:
(1) remove apical dominance, induction axillary bud development
A.8 at the beginning of the moon, select individual plant of switchgrass kind Alamo of robust growth.
B. switchgrass is cut apart from ground 50～60cm eminence, left 3～4 joints in below.
(2) culture matrix is prepared
From flower shed, buy flower culturing soil culture matrix, composition is 2 parts of cultivating soils (garden mould), 6 parts of leaf moulds, 2 parts of river sands.
(3) draw materials
A. when switchgrass top was cut after 20～30 days, top axillalry bud can grow into the long lateral bud of 15～20cm.
B. by lateral bud its together with its place joint up and down each 3～5cm from plant, cut.
(4) cutting is processed
A. peel off the blade that is wrapped in lateral bud on cutting base portion maternal plant, and cutting lower end is whittled into wedge shape, so that cuttage.
B. the cutting bottom after pruning is immersed in to mass concentration and is 24h in 0.1% TDZ rooting powder solution.
(5): cuttage is cultivated
A. the cutting after processing is inserted and is equipped with in the culturing pot of culture matrix, culturing pot is placed in basin, and adds water in basin, makes culture matrix from bottom, start immersion, until it is saturated to absorb water.
B. culturing pot is placed in 20 ℃ of constant temperature greenhouses and cultivates, about one week, it is long that new root can grow to 10～20cm.
(6): transplant
The cutting of having taken root in culturing pot is taken out, and in the large flowerpot that again to transplant into off-the-shelf bore in greenhouse be 25cm, so far, cutting can normal growth.
Claims (5)
1. a switchgrass cuttage asexual reproduction method, is characterized in that, comprises the following steps:
1) after switchgrass grows 4～6 joints, remove and tiller top and retain 3～4 joints in below, impel axillalry bud to germinate; The top of tillering was cut after 20～30 days, and axillary bud growth becomes the long lateral bud of 15～20cm;
2) lateral bud is saved and saved each 3～5cm up and down together with its place and cut from switchgrass plant, as cutting; Peel off the blade that wraps up lateral bud on cutting base portion maternal plant, and cutting lower end is whittled into wedge shape;
3) the cutting bottom after pruning is immersed in rooting powder solution more than 12h, then cutting is inserted in the culturing pot that loose permeable culture matrix is housed, culturing pot is placed in to water, make culture matrix from bottom, start immersion, until culture matrix water suction is saturated; Then culturing pot is placed in constant temperature greenhouse and is cultivated, treat that new root grows to 10～20cm long;
In volume parts, described culture matrix is that 1～2 part of 1～2 part of cultivating soil, 4～6 parts of leaf moulds and river sand is fully mixed and obtained;
It is to cultivate 6～8 days in the constant temperature greenhouse of 20～25 ℃ that described culturing pot is placed to temperature;
4) cutting of having taken root in culturing pot is taken out, transplant in greenhouse.
2. switchgrass cuttage asexual reproduction method as claimed in claim 1, it is characterized in that, the described removal top of tillering is in 7～August, treats that switchgrass grows 4～6 internodes that extend completely, the individual plant of the switchgrass of selected robust growth, except 3～4 joints in top reservation below of tillering.
3. switchgrass cuttage asexual reproduction method as claimed in claim 1, it is characterized in that, after lateral bud is cut as cutting from switchgrass, the axillalry bud that the below of lateral bud place joint is close on joint germinates, after this axillary bud growth to 15～20cm is long, using it as cutting, carries out second and take turns cuttage.
4. switchgrass cuttage asexual reproduction method as claimed in claim 1, is characterized in that, described root-inducing powder is the TDZ root-inducing powder of 500～1000 times of dilutions, after rooting powder solution soaks 24h, carries out cuttage.
5. switchgrass cuttage asexual reproduction method as claimed in claim 1, is characterized in that, the kind of described switchgrass is Alamo.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310218910.2A CN103299806B (en) | 2013-06-04 | 2013-06-04 | Switchgrass cuttage vegetative propagation method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310218910.2A CN103299806B (en) | 2013-06-04 | 2013-06-04 | Switchgrass cuttage vegetative propagation method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103299806A CN103299806A (en) | 2013-09-18 |
| CN103299806B true CN103299806B (en) | 2014-12-10 |
Family
ID=49125874
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310218910.2A Expired - Fee Related CN103299806B (en) | 2013-06-04 | 2013-06-04 | Switchgrass cuttage vegetative propagation method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103299806B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104041269B (en) * | 2014-05-12 | 2016-08-24 | 宁夏大学 | A kind of be full of cracks alkaline earth switchgrass quick planting and establishing method |
| CN104115651A (en) * | 2014-07-04 | 2014-10-29 | 中国科学院东北地理与农业生态研究所 | Rapid propagation method for cuttage of salix brachypoda |
| CN105052500A (en) * | 2015-08-20 | 2015-11-18 | 中国科学院东北地理与农业生态研究所 | Method for rapid propagation of switchgrass through rhizome transplantation |
| CN107006259A (en) * | 2017-04-28 | 2017-08-04 | 凤冈县领益昕中药材种植有限公司 | The mating system of lucid asparagus |
| CN109005973A (en) * | 2018-08-27 | 2018-12-18 | 山东省林业科学研究院 | A method of improving dryland willow cutting plantation |
| CN115462303A (en) * | 2022-09-06 | 2022-12-13 | 北京恒丰亚业科技发展有限公司 | Seedling raising method for gramineous plants |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
-
2013
- 2013-06-04 CN CN201310218910.2A patent/CN103299806B/en not_active Expired - Fee Related
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101946617A (en) * | 2010-08-23 | 2011-01-19 | 湖南农业大学 | Cottage and rapid propagation method of perennial energy grass |
Non-Patent Citations (4)
| Title |
|---|
| 两种沙性栽培基质下柳枝稷根系生长对施氮水平的响应;朱毅;《中国草地学报》;20120325;全文 * |
| 朱毅.两种沙性栽培基质下柳枝稷根系生长对施氮水平的响应.《中国草地学报》.2012,全文. * |
| 能源作物柳枝稷栽培技术;黄楠;《现代农业科技》;20090910;全文 * |
| 黄楠.能源作物柳枝稷栽培技术.《现代农业科技》.2009,全文. * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103299806A (en) | 2013-09-18 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103299806B (en) | Switchgrass cuttage vegetative propagation method | |
| CN103749302A (en) | Induced acclimation and cultivation method for salt-tolerant bamboo reed seedlings | |
| CN101263787A (en) | Louis iris tissue culture fast seedling establishment and ecological application method | |
| CN103461121A (en) | Ex-vitro rooting method for tissue culture seedlings of pinus massoniana | |
| CN105638220A (en) | Cutting propagation method for magnolia soulangeana | |
| CN105191792B (en) | The rapid propagation method of almond ringdove chrysanthemum | |
| CN104472365B (en) | Rich and honour banyan tissue-culturing rapid propagation method for culturing seedlings | |
| CN102613051A (en) | Method for plant rapid propagation through air culture | |
| CN104686116B (en) | Energy crop reed topping cuttage breeding method | |
| CN103782811A (en) | Watermelon tissue culture seedling test tube micro-grafting method | |
| CN103609453B (en) | A kind of construction method of tea tree vitro Regeneration System | |
| CN102919132B (en) | Tissue culture method for inducing populus yunnanensis dode tetraploid in combination with colchicine | |
| CN103168692B (en) | Salix saposhnikovii tissue culture method | |
| CN107027627A (en) | A kind of micro-tuber propagation method of David's-harp IMMATURE EMBRYOS CULTURE | |
| CN104938335B (en) | The method that regeneration plant is obtained using oil tea hypocotyls | |
| CN101637130B (en) | Cephalotaxus hainanensis embryo culturing and seedling breeding method | |
| CN103907497A (en) | Rapid cutting propagation method of test-tube plum plantlets | |
| CN101766123B (en) | Method for rapid propagation of zephyr lily | |
| CN104686344A (en) | Tissue culture method of liriope muscari | |
| CN105638456B (en) | A kind of few survivors seeds yew seed tissue culture outside sprout-cultivating-bottle radication cultural method in imminent danger | |
| CN104094848A (en) | Induction of tung tree hypocotyls callus and method for efficiently regenerating plants | |
| CN103039370B (en) | Method for establishing golden-edge arc-leaf maguey isolated cultivation and regeneration system | |
| CN104272949A (en) | Crassulaceae plant cultivating and seedling method | |
| CN101449658B (en) | Green cucumber high-frequency regeneration method | |
| CN103416303A (en) | Inducing method for spring dendrobium protoemm-like body |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141210 Termination date: 20150604 |
|
| EXPY | Termination of patent right or utility model |