BR112015029985A2 - método para produção de l-leucina, l-valina, l-isoleucina, alfa-cetoisovalerato, alfa-ceto-beta-metilvalerato, ou alfa-cetoisocaproato usando corynebactéria recombinante que contém ilvbn operon, o qual pode ser induzido por propionato - Google Patents

método para produção de l-leucina, l-valina, l-isoleucina, alfa-cetoisovalerato, alfa-ceto-beta-metilvalerato, ou alfa-cetoisocaproato usando corynebactéria recombinante que contém ilvbn operon, o qual pode ser induzido por propionato

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Publication number
BR112015029985A2
BR112015029985A2 BR112015029985A BR112015029985A BR112015029985A2 BR 112015029985 A2 BR112015029985 A2 BR 112015029985A2 BR 112015029985 A BR112015029985 A BR 112015029985A BR 112015029985 A BR112015029985 A BR 112015029985A BR 112015029985 A2 BR112015029985 A2 BR 112015029985A2
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BR
Brazil
Prior art keywords
alpha
propionate
ketoisocaproate
ketoisovalerate
methylvalerate
Prior art date
Application number
BR112015029985A
Other languages
English (en)
Inventor
Ramos-Vera Hugo
Marin Kay
Gerstmeir Robert
Original Assignee
Evonik Degussa Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Evonik Degussa Gmbh filed Critical Evonik Degussa Gmbh
Publication of BR112015029985A2 publication Critical patent/BR112015029985A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1022—Transferases (2.) transferring aldehyde or ketonic groups (2.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y202/00—Transferases transferring aldehyde or ketonic groups (2.2)
    • C12Y202/01—Transketolases and transaldolases (2.2.1)
    • C12Y202/01006—Acetolactate synthase (2.2.1.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y401/00—Carbon-carbon lyases (4.1)
    • C12Y401/03—Oxo-acid-lyases (4.1.3)

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

resumo “método para produção de l-leucina, l-valina, l-isoleucina, alfa-cetoisovalerato, alfa-ceto-beta-metilvalerato, ou alfa-cetoisocaproato usando corynebactéria recombinante que contém ilvbn operon, o qual pode ser induzido por propionato” a invenção refere-se a um processo para a produção de aminoácidos e cetoácidos usando microorganismos, em que um promotor indutível por propionato torna possível a expressão regulada de determinados genes.
BR112015029985A 2013-06-03 2014-05-23 método para produção de l-leucina, l-valina, l-isoleucina, alfa-cetoisovalerato, alfa-ceto-beta-metilvalerato, ou alfa-cetoisocaproato usando corynebactéria recombinante que contém ilvbn operon, o qual pode ser induzido por propionato BR112015029985A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP13170248.2A EP2811028B1 (de) 2013-06-03 2013-06-03 Verfahren zur Herstellung von L-Valin unter Verwendung rekombinanter Corynebakterien enthaltend das durch Propionat induzierbare ilvBN-Operon
PCT/EP2014/060680 WO2014195154A1 (de) 2013-06-03 2014-05-23 Verfahren zur herstellung von l-leucin, l-valin, l-isoleucin, alpha-ketoisovalerat, alpha-keto-beta-methylvalerat oder alpha-ketoisocaproat unter verwendung rekombinanter corynebakterien enthaltend das durch propionat induzierbare ilvbn-operon

Publications (1)

Publication Number Publication Date
BR112015029985A2 true BR112015029985A2 (pt) 2017-09-26

Family

ID=48537853

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112015029985A BR112015029985A2 (pt) 2013-06-03 2014-05-23 método para produção de l-leucina, l-valina, l-isoleucina, alfa-cetoisovalerato, alfa-ceto-beta-metilvalerato, ou alfa-cetoisocaproato usando corynebactéria recombinante que contém ilvbn operon, o qual pode ser induzido por propionato

Country Status (12)

Country Link
US (1) US10113190B2 (pt)
EP (1) EP2811028B1 (pt)
JP (1) JP2016519949A (pt)
KR (1) KR20160015298A (pt)
CN (1) CN105492616A (pt)
BR (1) BR112015029985A2 (pt)
DK (1) DK2811028T3 (pt)
ES (1) ES2623161T3 (pt)
HU (1) HUE032752T2 (pt)
PL (1) PL2811028T3 (pt)
RU (1) RU2675506C2 (pt)
WO (1) WO2014195154A1 (pt)

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EP3415622A1 (en) 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
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KR101996129B1 (ko) 2017-07-11 2019-07-04 씨제이제일제당 (주) 아세토하이드록시산 신타아제 변이체, 이를 포함하는 미생물 또는 이를 이용하는 l-분지쇄 아미노산 생산 방법
EP3456833A1 (en) 2017-09-18 2019-03-20 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
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EP3467099A1 (en) 2017-10-05 2019-04-10 Evonik Degussa GmbH Method for the fermentative production of l-amino acids
CN108753810B (zh) * 2018-05-22 2021-06-18 昆明理工大学 一种转录调节蛋白基因orf2的用途
KR102134418B1 (ko) * 2019-06-17 2020-07-16 씨제이제일제당 주식회사 L-타이로신을 생산하는 미생물 및 이를 이용한 l-타이로신 생산 방법
WO2021037190A1 (zh) * 2019-08-29 2021-03-04 天津科技大学 一种2-异丙基苹果酸合成酶及其工程菌与应用
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KR102470602B1 (ko) 2020-12-11 2022-11-25 씨제이제일제당 주식회사 신규한 분지 연쇄 아미노산 아미노트렌스퍼라아제 변이체 및 이를 이용한 이소류신 생산 방법
KR102464883B1 (ko) 2020-12-11 2022-11-09 씨제이제일제당 주식회사 신규한 감마-아미노부티르산 퍼미에이즈 변이체 및 이를 이용한 이소류신 생산 방법
CN113683666A (zh) * 2021-08-23 2021-11-23 黑龙江伊品生物科技有限公司 Yh66-rs07020基因改造得到的工程菌及其在制备缬氨酸中的应用
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KR102673796B1 (ko) 2021-09-29 2024-06-10 씨제이제일제당 주식회사 신규한 아세토하이드록시산 신테아제 변이체 및 이를 이용한 l-이소류신 생산방법

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Also Published As

Publication number Publication date
CN105492616A (zh) 2016-04-13
DK2811028T3 (en) 2017-05-01
WO2014195154A1 (de) 2014-12-11
RU2015156852A (ru) 2017-07-17
JP2016519949A (ja) 2016-07-11
US20160115506A1 (en) 2016-04-28
RU2015156852A3 (pt) 2018-04-02
EP2811028B1 (de) 2017-02-01
US10113190B2 (en) 2018-10-30
HUE032752T2 (en) 2017-10-30
EP2811028A1 (de) 2014-12-10
ES2623161T3 (es) 2017-07-10
RU2675506C2 (ru) 2018-12-20
PL2811028T3 (pl) 2017-07-31
KR20160015298A (ko) 2016-02-12

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