BR112015015770A2 - cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas - Google Patents

cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas

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Publication number
BR112015015770A2
BR112015015770A2 BR112015015770A BR112015015770A BR112015015770A2 BR 112015015770 A2 BR112015015770 A2 BR 112015015770A2 BR 112015015770 A BR112015015770 A BR 112015015770A BR 112015015770 A BR112015015770 A BR 112015015770A BR 112015015770 A2 BR112015015770 A2 BR 112015015770A2
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BR
Brazil
Prior art keywords
air
liquid interface
differentiation
cells
human embryonic
Prior art date
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BR112015015770A
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English (en)
Inventor
Rezania Alireza
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Janssen Biotech Inc
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First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51017432&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BR112015015770(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of BR112015015770A2 publication Critical patent/BR112015015770A2/pt

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/507—Pancreatic cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/15—Transforming growth factor beta (TGF-β)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue

Abstract

abstract the present invention provides methods, cell cultures and differentiation media to promote differentiation of pluripotent stem cells to pancreatic endocrine cells expressing pdx1, nkx6.1, and hb9 by culturing in a culture vessel at the air-liquid interface. the invention also provides for in vivo maturation of cells cultured at the air-liquid interface. tradução do resumo resumo patente de invenção: "cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas". a presente invenção fornece métodos, culturas celulares e meios de diferenciação para promover a diferenciação de células-tronco pluripotentes para células pancreáticas endócrinas que expressam pdx1, nkx6.1 e hb9 pelo cultivo em um vaso de cultura na interface ar-líquido. a invenção também fornece a maturação in vivo de células cultivadas na interface ar-líquido. 17/17 94 erro! nome de propriedade do documento desconhecido.
BR112015015770A 2012-12-31 2013-12-18 cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas BR112015015770A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261747662P 2012-12-31 2012-12-31
PCT/US2013/075939 WO2014105543A1 (en) 2012-12-31 2013-12-18 Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells

Publications (1)

Publication Number Publication Date
BR112015015770A2 true BR112015015770A2 (pt) 2017-07-11

Family

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BR112015015770A BR112015015770A2 (pt) 2012-12-31 2013-12-18 cultivo de células-tronco embrionárias humanas na interface ar-líquido para diferenciação em células pancreáticas endócrinas

Country Status (17)

Country Link
US (1) US10344264B2 (pt)
EP (1) EP2938724B1 (pt)
JP (1) JP6557146B2 (pt)
KR (1) KR102084561B1 (pt)
CN (1) CN105008518B (pt)
AR (1) AR094347A1 (pt)
AU (4) AU2013368221B2 (pt)
BR (1) BR112015015770A2 (pt)
CA (1) CA2896655C (pt)
DK (1) DK2938724T3 (pt)
ES (1) ES2837763T3 (pt)
HK (1) HK1217110A1 (pt)
MX (1) MX2015008577A (pt)
PH (1) PH12015501465A1 (pt)
RU (3) RU2658488C2 (pt)
SG (2) SG11201505128SA (pt)
WO (1) WO2014105543A1 (pt)

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