SG10201709338RA - Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells - Google Patents

Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells

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Publication number
SG10201709338RA
SG10201709338RA SG10201709338RA SG10201709338RA SG10201709338RA SG 10201709338R A SG10201709338R A SG 10201709338RA SG 10201709338R A SG10201709338R A SG 10201709338RA SG 10201709338R A SG10201709338R A SG 10201709338RA SG 10201709338R A SG10201709338R A SG 10201709338RA
Authority
SG
Singapore
Prior art keywords
culturing
differentiation
air
embryonic stem
human embryonic
Prior art date
Application number
SG10201709338RA
Inventor
Alireza Rezania
Original Assignee
Janssen Biotech Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=51017432&utm_source=***_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG10201709338R(A) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Janssen Biotech Inc filed Critical Janssen Biotech Inc
Publication of SG10201709338RA publication Critical patent/SG10201709338RA/en

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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0676—Pancreatic cells
    • C—CHEMISTRY; METALLURGY
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    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0603—Embryonic cells ; Embryoid bodies
    • C12N5/0606—Pluripotent embryonic cells, e.g. embryonic stem cells [ES]
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/5044—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics involving specific cell types
    • G01N33/507—Pancreatic cells
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    • C12N2500/00—Specific components of cell culture medium
    • C12N2500/05—Inorganic components
    • C12N2500/10—Metals; Metal chelators
    • C12N2500/20—Transition metals
    • C12N2500/24—Iron; Fe chelators; Transferrin
    • C12N2500/25—Insulin-transferrin; Insulin-transferrin-selenium
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/115—Basic fibroblast growth factor (bFGF, FGF-2)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
    • C12N2501/117—Keratinocyte growth factors (KGF-1, i.e. FGF-7; KGF-2, i.e. FGF-12)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/10—Growth factors
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/30—Hormones
    • C12N2501/38—Hormones with nuclear receptors
    • C12N2501/385—Hormones with nuclear receptors of the family of the retinoic acid recptor, e.g. RAR, RXR; Peroxisome proliferator-activated receptor [PPAR]
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/70—Enzymes
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    • C12N2501/727—Kinases (EC 2.7.)
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    • C12N2501/00—Active agents used in cell culture processes, e.g. differentation
    • C12N2501/999—Small molecules not provided for elsewhere
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    • C12N2506/00—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells
    • C12N2506/02—Differentiation of animal cells from one lineage to another; Differentiation of pluripotent cells from embryonic cells
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/30—Synthetic polymers
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    • C12N2533/00—Supports or coatings for cell culture, characterised by material
    • C12N2533/90—Substrates of biological origin, e.g. extracellular matrix, decellularised tissue
SG10201709338RA 2012-12-31 2013-12-18 Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells SG10201709338RA (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US201261747662P 2012-12-31 2012-12-31

Publications (1)

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SG10201709338RA true SG10201709338RA (en) 2017-12-28

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SG10201709338RA SG10201709338RA (en) 2012-12-31 2013-12-18 Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells
SG11201505128SA SG11201505128SA (en) 2012-12-31 2013-12-18 Culturing of human embryonic stem cells at the air-liquid interface for differentiation into pancreatic endocrine cells

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Country Status (17)

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US (1) US10344264B2 (en)
EP (1) EP2938724B1 (en)
JP (1) JP6557146B2 (en)
KR (1) KR102084561B1 (en)
CN (1) CN105008518B (en)
AR (1) AR094347A1 (en)
AU (4) AU2013368221B2 (en)
BR (1) BR112015015770A2 (en)
CA (1) CA2896655C (en)
DK (1) DK2938724T3 (en)
ES (1) ES2837763T3 (en)
HK (1) HK1217110A1 (en)
MX (1) MX2015008577A (en)
PH (1) PH12015501465A1 (en)
RU (3) RU2018116647A (en)
SG (2) SG10201709338RA (en)
WO (1) WO2014105543A1 (en)

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