WO2005014633A8 - 蛍光蛋白質 - Google Patents

蛍光蛋白質

Info

Publication number
WO2005014633A8
WO2005014633A8 PCT/JP2004/011870 JP2004011870W WO2005014633A8 WO 2005014633 A8 WO2005014633 A8 WO 2005014633A8 JP 2004011870 W JP2004011870 W JP 2004011870W WO 2005014633 A8 WO2005014633 A8 WO 2005014633A8
Authority
WO
WIPO (PCT)
Prior art keywords
fluorescent protein
calcium
apoprotein
ion
protein
Prior art date
Application number
PCT/JP2004/011870
Other languages
English (en)
French (fr)
Other versions
WO2005014633A1 (ja
Inventor
Satoshi Inouye
Original Assignee
Chisso Corp
Satoshi Inouye
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chisso Corp, Satoshi Inouye filed Critical Chisso Corp
Priority to AT04771831T priority Critical patent/ATE488527T1/de
Priority to US10/567,857 priority patent/US8710195B2/en
Priority to EP04771831A priority patent/EP1666488B1/en
Priority to JP2005513049A priority patent/JP4770462B2/ja
Priority to DE602004030136T priority patent/DE602004030136D1/de
Publication of WO2005014633A1 publication Critical patent/WO2005014633A1/ja
Publication of WO2005014633A8 publication Critical patent/WO2005014633A8/ja

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/43504—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates
    • C07K14/43595—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from invertebrates from coelenteratae, e.g. medusae
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
    • G01N21/76—Chemiluminescence; Bioluminescence
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/84—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving inorganic compounds or pH

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Hematology (AREA)
  • General Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Pathology (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Plasma & Fusion (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Inorganic Chemistry (AREA)
  • Toxicology (AREA)
  • Biotechnology (AREA)
  • Cell Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Genetics & Genomics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Food Science & Technology (AREA)
  • Peptides Or Proteins (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Luminescent Compositions (AREA)
  • Electroluminescent Light Sources (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analyzing Non-Biological Materials By The Use Of Chemical Means (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

化学発光活性を有する蛍光蛋白質（ｂＦＰ）は、カルシウム結合型発光蛋白質のアポ蛋白質、セレンテラミドまたはその類縁化合物、およびカルシウムイオンまたはカルシウムイオンと置換可能な２価もしくは３価のイオンより構成された複合体であり、複合体中のアポ蛋白質とセレンテラミドの分子数の比が１：１であり、アポ蛋白質と２価もしくは３価のイオンの分子数の比が１：１～４である。これは、セレンテラジンの発光を触媒し、かつ蛍光発光能を有するので、マーカーとして利用される。この発光活性を有する蛍光蛋白質（ｂＦＰ）から、カルシウムイオン等を除去すると新規の蛍光蛋白質（ｇＦＰ）となる。これをセレンテラジンと混合するとカルシウム結合型発光蛋白質となり、カルシウムにより瞬間的に発光するので、マーカーとして利用できる。
PCT/JP2004/011870 2003-08-12 2004-08-12 蛍光蛋白質 WO2005014633A1 (ja)

Priority Applications (5)

Application Number Priority Date Filing Date Title
AT04771831T ATE488527T1 (de) 2003-08-12 2004-08-12 Fluoreszenzprotein
US10/567,857 US8710195B2 (en) 2003-08-12 2004-08-12 Fluorescent proteins
EP04771831A EP1666488B1 (en) 2003-08-12 2004-08-12 Fluorescent protein
JP2005513049A JP4770462B2 (ja) 2003-08-12 2004-08-12 蛍光蛋白質
DE602004030136T DE602004030136D1 (de) 2003-08-12 2004-08-12 Fluoreszenzprotein

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP2003-207397 2003-08-12
JP2003207397 2003-08-12
JP2004-059611 2004-03-03
JP2004059611 2004-03-03

Publications (2)

Publication Number Publication Date
WO2005014633A1 WO2005014633A1 (ja) 2005-02-17
WO2005014633A8 true WO2005014633A8 (ja) 2005-03-31

Family

ID=34137895

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/JP2004/011870 WO2005014633A1 (ja) 2003-08-12 2004-08-12 蛍光蛋白質

Country Status (6)

Country Link
US (1) US8710195B2 (ja)
EP (1) EP1666488B1 (ja)
JP (1) JP4770462B2 (ja)
AT (1) ATE488527T1 (ja)
DE (1) DE602004030136D1 (ja)
WO (1) WO2005014633A1 (ja)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7460129B2 (ja) 2020-04-03 2024-04-02 学校法人福岡大学 多色蛍光タンパク質を含む金属検出用試薬および被検液の金属検出方法、ならびに組み換えベクター、金属挙動の評価方法

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP4639904B2 (ja) * 2005-03-30 2011-02-23 チッソ株式会社 蛍光活性を有するルシフェラーゼの活性増強方法
JP4609177B2 (ja) * 2005-04-28 2011-01-12 チッソ株式会社 カルシウム結合型発光蛋白質溶液の発光時間延長方法
EP1928237A4 (en) 2005-09-02 2011-03-09 Abbott Lab NEW HETEROCYCLES BASED ON IMIDAZO
JP5130662B2 (ja) * 2006-06-07 2013-01-30 Ｊｎｃ株式会社 タンパク質の可溶性タンパク質としての製造方法
JP4952085B2 (ja) * 2006-06-22 2012-06-13 Ｊｎｃ株式会社 発光活性阻害方法及び微量重金属定量方法
JP5540501B2 (ja) * 2008-12-22 2014-07-02 Ｊｎｃ株式会社 発光蛋白質の製造方法
JP5353279B2 (ja) 2009-02-06 2013-11-27 Ｊｎｃ株式会社 セレンテラミド又はその類縁体の製造方法
US8431525B2 (en) * 2009-02-09 2013-04-30 Jnc Corporation Coelenteramide analogs
JP5605727B2 (ja) * 2010-04-06 2014-10-15 Ｊｎｃ株式会社 セレンテラジン類縁体及びセレンテラミド類縁体
KR101210914B1 (ko) 2010-11-10 2012-12-11 한국과학기술연구원 형광단백질 구조체를 이용한 분리막 오염도 정량분석 방법 및 장치
JP5637180B2 (ja) * 2012-06-26 2014-12-10 Jnc株式会社 タンパク質の可溶性タンパク質としての製造方法
US9624425B2 (en) 2012-09-01 2017-04-18 Prolume, Ltd. Substituted benzo[f]imidazo[1,2-a]quinoxalines and imidazo[1,2-a]pyrazines
JP5772892B2 (ja) * 2013-07-04 2015-09-02 Jnc株式会社 セレンテラミド又はその類縁体の製造方法
KR101640499B1 (ko) * 2015-08-19 2016-07-18 한림대학교 산학협력단 형질 전환 누에고치로부터 추출한 형광 실크 단백질 용액의 제조 방법 및 이를 이용한 지지체 제조 방법
WO2017151728A1 (en) * 2016-03-01 2017-09-08 Board Of Supervisors Of Louisiana State Univ. And Agricultural And Mechanical College Indirect assessment of insulin release in a cell

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS61135586A (ja) 1984-08-24 1986-06-23 Chisso Corp 発光蛋白アクアリン遺伝子を有するプラスミドおよび該発光蛋白の生合成法
JPS6447379A (en) 1987-08-19 1989-02-21 Chisso Corp Preparation of calcium-dependent oxygenation enzyme
JP2592623B2 (ja) 1987-11-18 1997-03-19 チッソ株式会社 アポエクオリンの製造法
US5876995A (en) 1996-02-06 1999-03-02 Bryan; Bruce Bioluminescent novelty items
JP4501211B2 (ja) 2000-03-24 2010-07-14 チッソ株式会社 発光量標準物質
JP4193499B2 (ja) * 2002-03-29 2008-12-10 チッソ株式会社 組換え発光蛋白質およびその複合体
JP4348903B2 (ja) 2002-07-02 2009-10-21 チッソ株式会社 カルシウム結合型蛋白質の濃縮物からなる連続発光可能な発光物質
JP4196607B2 (ja) 2002-07-29 2008-12-17 チッソ株式会社 リガンドと結合したイクオリンおよびその製造法
JP4232562B2 (ja) 2002-09-09 2009-03-04 チッソ株式会社 カルシウム結合型発光蛋白質が分散している吸水性高分子ゲル組成物、およびこれを含有する化粧料、並びにこれらの連続発光方法

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP7460129B2 (ja) 2020-04-03 2024-04-02 学校法人福岡大学 多色蛍光タンパク質を含む金属検出用試薬および被検液の金属検出方法、ならびに組み換えベクター、金属挙動の評価方法

Also Published As

Publication number Publication date
JP4770462B2 (ja) 2011-09-14
EP1666488A1 (en) 2006-06-07
WO2005014633A1 (ja) 2005-02-17
EP1666488B1 (en) 2010-11-17
US8710195B2 (en) 2014-04-29
JPWO2005014633A1 (ja) 2007-11-01
EP1666488A4 (en) 2007-10-03
ATE488527T1 (de) 2010-12-15
DE602004030136D1 (de) 2010-12-30
US20110070656A1 (en) 2011-03-24

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