NO328694B1 - Fremgangsmate for fremstilling av rekombinant hCG med spesifikk biologisk aktivitet fra en prove - Google Patents

Fremgangsmate for fremstilling av rekombinant hCG med spesifikk biologisk aktivitet fra en prove Download PDF

Info

Publication number: NO328694B1
Authority: NO; Norway
Prior art keywords: hcg; sample; column; eluting; exchange chromatography
Prior art date: 2000-02-22

Application number

NO20023985A

Other languages

English (en)

Norwegian (no)

Other versions

NO20023985L (no

NO20023985D0 (no

Inventor

Gianfranco Paradisi

Mara Rossi

Laura Scaglia

Original Assignee

Serono Lab

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

2000-02-22

Filing date

2002-08-21

Publication date

2010-04-26

2002-08-21 Application filed by Serono Lab filed Critical Serono Lab

2002-08-21 Publication of NO20023985L publication Critical patent/NO20023985L/no

2002-08-21 Publication of NO20023985D0 publication Critical patent/NO20023985D0/no

2010-04-26 Publication of NO328694B1 publication Critical patent/NO328694B1/no

Links

238000000034 method Methods 0.000 title claims abstract description 41
230000004071 biological effect Effects 0.000 title claims description 5
238000002360 preparation method Methods 0.000 title description 18
238000004255 ion exchange chromatography Methods 0.000 claims abstract description 19
238000004007 reversed phase HPLC Methods 0.000 claims abstract description 16
238000001542 size-exclusion chromatography Methods 0.000 claims abstract description 7
210000004978 chinese hamster ovary cell Anatomy 0.000 claims abstract description 3
VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 16
238000004587 chromatography analysis Methods 0.000 claims description 12
238000004519 manufacturing process Methods 0.000 claims description 10
239000012064 sodium phosphate buffer Substances 0.000 claims description 10
238000010828 elution Methods 0.000 claims description 8
239000001963 growth medium Substances 0.000 claims description 8
239000000377 silicon dioxide Substances 0.000 claims description 8
ATRRKUHOCOJYRX-UHFFFAOYSA-N Ammonium bicarbonate Chemical compound [NH4+].OC([O-])=O ATRRKUHOCOJYRX-UHFFFAOYSA-N 0.000 claims description 7
229910000013 Ammonium bicarbonate Inorganic materials 0.000 claims description 7
229920002271 DEAE-Sepharose Polymers 0.000 claims description 7
229920002684 Sepharose Polymers 0.000 claims description 7
235000012538 ammonium bicarbonate Nutrition 0.000 claims description 7
239000001099 ammonium carbonate Substances 0.000 claims description 7
239000012506 Sephacryl® Substances 0.000 claims description 5
239000000872 buffer Substances 0.000 claims description 5
JLEXUIVKURIPFI-UHFFFAOYSA-N tris phosphate Chemical compound OP(O)(O)=O.OCC(N)(CO)CO JLEXUIVKURIPFI-UHFFFAOYSA-N 0.000 claims description 5
239000008363 phosphate buffer Substances 0.000 claims description 3
102000011022 Chorionic Gonadotropin Human genes 0.000 abstract description 39
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239000000356 contaminant Substances 0.000 abstract description 11
239000006228 supernatant Substances 0.000 abstract description 2
KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 description 27
239000000243 solution Substances 0.000 description 15
102000004169 proteins and genes Human genes 0.000 description 14
108090000623 proteins and genes Proteins 0.000 description 14
239000000047 product Substances 0.000 description 12
238000000108 ultra-filtration Methods 0.000 description 12
HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 description 9
210000002700 urine Anatomy 0.000 description 9
238000011210 chromatographic step Methods 0.000 description 8
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235000011008 sodium phosphates Nutrition 0.000 description 8
RYFMWSXOAZQYPI-UHFFFAOYSA-K trisodium phosphate Chemical compound [Na+].[Na+].[Na+].[O-]P([O-])([O-])=O RYFMWSXOAZQYPI-UHFFFAOYSA-K 0.000 description 8
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229910000147 aluminium phosphate Inorganic materials 0.000 description 3
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238000010586 diagram Methods 0.000 description 3
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238000004128 high performance liquid chromatography Methods 0.000 description 3
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239000005556 hormone Substances 0.000 description 3
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238000000926 separation method Methods 0.000 description 3
238000002415 sodium dodecyl sulfate polyacrylamide gel electrophoresis Methods 0.000 description 3
FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
102000006771 Gonadotropins Human genes 0.000 description 2
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108010073521 Luteinizing Hormone Proteins 0.000 description 2
102000009151 Luteinizing Hormone Human genes 0.000 description 2
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102000007056 Recombinant Fusion Proteins Human genes 0.000 description 2
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238000004166 bioassay Methods 0.000 description 2
125000002057 carboxymethyl group Chemical group [H]OC(=O)C([H])([H])[*] 0.000 description 2
239000006143 cell culture medium Substances 0.000 description 2
238000005352 clarification Methods 0.000 description 2
239000012228 culture supernatant Substances 0.000 description 2
238000000502 dialysis Methods 0.000 description 2
239000002622 gonadotropin Substances 0.000 description 2
239000000833 heterodimer Substances 0.000 description 2
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238000000338 in vitro Methods 0.000 description 2
238000010255 intramuscular injection Methods 0.000 description 2
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238000003860 storage Methods 0.000 description 2
NWUYHJFMYQTDRP-UHFFFAOYSA-N 1,2-bis(ethenyl)benzene;1-ethenyl-2-ethylbenzene;styrene Chemical compound C=CC1=CC=CC=C1.CCC1=CC=CC=C1C=C.C=CC1=CC=CC=C1C=C NWUYHJFMYQTDRP-UHFFFAOYSA-N 0.000 description 1
PYTMYKVIJXPNBD-OQKDUQJOSA-N 2-[4-[(z)-2-chloro-1,2-diphenylethenyl]phenoxy]-n,n-diethylethanamine;hydron;2-hydroxypropane-1,2,3-tricarboxylate Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O.C1=CC(OCCN(CC)CC)=CC=C1C(\C=1C=CC=CC=1)=C(/Cl)C1=CC=CC=C1 PYTMYKVIJXPNBD-OQKDUQJOSA-N 0.000 description 1
NLXLAEXVIDQMFP-UHFFFAOYSA-N Ammonium chloride Substances [NH4+].[Cl-] NLXLAEXVIDQMFP-UHFFFAOYSA-N 0.000 description 1
VHUUQVKOLVNVRT-UHFFFAOYSA-N Ammonium hydroxide Chemical compound [NH4+].[OH-] VHUUQVKOLVNVRT-UHFFFAOYSA-N 0.000 description 1
102000004506 Blood Proteins Human genes 0.000 description 1
241000283690 Bos taurus Species 0.000 description 1
241000167854 Bourreria succulenta Species 0.000 description 1
SGHZXLIDFTYFHQ-UHFFFAOYSA-L Brilliant Blue Chemical compound [Na+].[Na+].C=1C=C(C(=C2C=CC(C=C2)=[N+](CC)CC=2C=C(C=CC=2)S([O-])(=O)=O)C=2C(=CC=CC=2)S([O-])(=O)=O)C=CC=1N(CC)CC1=CC=CC(S([O-])(=O)=O)=C1 SGHZXLIDFTYFHQ-UHFFFAOYSA-L 0.000 description 1
241000282693 Cercopithecidae Species 0.000 description 1
206010011498 Cryptorchism Diseases 0.000 description 1
206010012205 Delayed puberty Diseases 0.000 description 1
241000283086 Equidae Species 0.000 description 1
LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 1
108010079345 Follicle Stimulating Hormone Proteins 0.000 description 1
102000012673 Follicle Stimulating Hormone Human genes 0.000 description 1
241001622557 Hesperia Species 0.000 description 1
208000007466 Male Infertility Diseases 0.000 description 1
241001494479 Pecora Species 0.000 description 1
241000282887 Suidae Species 0.000 description 1
206010042573 Superovulation Diseases 0.000 description 1
ZMANZCXQSJIPKH-UHFFFAOYSA-N Triethylamine Chemical compound CCN(CC)CC ZMANZCXQSJIPKH-UHFFFAOYSA-N 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
239000004480 active ingredient Substances 0.000 description 1
235000011114 ammonium hydroxide Nutrition 0.000 description 1
239000002269 analeptic agent Substances 0.000 description 1
239000003957 anion exchange resin Substances 0.000 description 1
230000002513 anti-ovulatory effect Effects 0.000 description 1
206010003883 azoospermia Diseases 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
239000012620 biological material Substances 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
244000309464 bull Species 0.000 description 1
230000015556 catabolic process Effects 0.000 description 1
238000005341 cation exchange Methods 0.000 description 1
239000003729 cation exchange resin Substances 0.000 description 1
239000003153 chemical reaction reagent Substances 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
235000019693 cherries Nutrition 0.000 description 1
238000004140 cleaning Methods 0.000 description 1
229940046989 clomiphene citrate Drugs 0.000 description 1
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239000012468 concentrated sample Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
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ATDGTVJJHBUTRL-UHFFFAOYSA-N cyanogen bromide Chemical compound BrC#N ATDGTVJJHBUTRL-UHFFFAOYSA-N 0.000 description 1
238000006731 degradation reaction Methods 0.000 description 1
229940075894 denatured ethanol Drugs 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
238000002086 displacement chromatography Methods 0.000 description 1
239000003814 drug Substances 0.000 description 1
230000008030 elimination Effects 0.000 description 1
238000003379 elimination reaction Methods 0.000 description 1
239000002158 endotoxin Substances 0.000 description 1
239000006167 equilibration buffer Substances 0.000 description 1
230000004720 fertilization Effects 0.000 description 1
229940028334 follicle stimulating hormone Drugs 0.000 description 1
230000008217 follicular development Effects 0.000 description 1
229940094892 gonadotropins Drugs 0.000 description 1
238000003306 harvesting Methods 0.000 description 1
201000003368 hypogonadotropic hypogonadism Diseases 0.000 description 1
208000000509 infertility Diseases 0.000 description 1
230000036512 infertility Effects 0.000 description 1
231100000535 infertility Toxicity 0.000 description 1
229940040129 luteinizing hormone Drugs 0.000 description 1
238000005374 membrane filtration Methods 0.000 description 1
230000003278 mimic effect Effects 0.000 description 1
229910000403 monosodium phosphate Inorganic materials 0.000 description 1
235000019799 monosodium phosphate Nutrition 0.000 description 1
208000008634 oligospermia Diseases 0.000 description 1
230000036616 oligospermia Effects 0.000 description 1
231100000528 oligospermia Toxicity 0.000 description 1
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210000002826 placenta Anatomy 0.000 description 1
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239000008213 purified water Substances 0.000 description 1
238000004366 reverse phase liquid chromatography Methods 0.000 description 1
230000000630 rising effect Effects 0.000 description 1
239000011780 sodium chloride Substances 0.000 description 1
AJPJDKMHJJGVTQ-UHFFFAOYSA-M sodium dihydrogen phosphate Chemical compound [Na+].OP(O)([O-])=O AJPJDKMHJJGVTQ-UHFFFAOYSA-M 0.000 description 1
239000007790 solid phase Substances 0.000 description 1
241000894007 species Species 0.000 description 1
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238000007920 subcutaneous administration Methods 0.000 description 1
125000000185 sucrose group Chemical group 0.000 description 1
239000013589 supplement Substances 0.000 description 1
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230000001225 therapeutic effect Effects 0.000 description 1
230000014616 translation Effects 0.000 description 1
LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
238000000870 ultraviolet spectroscopy Methods 0.000 description 1
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238000005406 washing Methods 0.000 description 1
238000001262 western blot Methods 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/36—Extraction; Separation; Purification by a combination of two or more processes of different types
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/59—Follicle-stimulating hormone [FSH]; Chorionic gonadotropins, e.g.hCG [human chorionic gonadotropin]; Luteinising hormone [LH]; Thyroid-stimulating hormone [TSH]
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P15/00—Drugs for genital or sexual disorders; Contraceptives
- A61P15/08—Drugs for genital or sexual disorders; Contraceptives for gonadal disorders or for enhancing fertility, e.g. inducers of ovulation or of spermatogenesis
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P5/00—Drugs for disorders of the endocrine system

Landscapes

Health & Medical Sciences (AREA)
Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Endocrinology (AREA)
Medicinal Chemistry (AREA)
General Health & Medical Sciences (AREA)
Reproductive Health (AREA)
Pharmacology & Pharmacy (AREA)
Public Health (AREA)
Biophysics (AREA)
Proteomics, Peptides & Aminoacids (AREA)
General Chemical & Material Sciences (AREA)
Animal Behavior & Ethology (AREA)
Chemical Kinetics & Catalysis (AREA)
Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
Veterinary Medicine (AREA)
Bioinformatics & Cheminformatics (AREA)
Engineering & Computer Science (AREA)
Molecular Biology (AREA)
Genetics & Genomics (AREA)
Biochemistry (AREA)
Toxicology (AREA)
Gastroenterology & Hepatology (AREA)
Zoology (AREA)
Diabetes (AREA)
Gynecology & Obstetrics (AREA)
Pregnancy & Childbirth (AREA)
Analytical Chemistry (AREA)
Peptides Or Proteins (AREA)
Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Preparation Of Compounds By Using Micro-Organisms (AREA)
Treatment Of Liquids With Adsorbents In General (AREA)
Compounds Of Unknown Constitution (AREA)
Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
Detergent Compositions (AREA)

NO20023985A 2000-02-22 2002-08-21 Fremgangsmate for fremstilling av rekombinant hCG med spesifikk biologisk aktivitet fra en prove NO328694B1 (no)

Applications Claiming Priority (2)

Application Number	Priority Date	Filing Date	Title
EP00103690		2000-02-22
PCT/EP2001/000665 WO2001062773A1 (en)	2000-02-22	2001-01-22	PROCESS OF PURIFICATION OF hCG AND RECOMBINANT hCG PURIFIED BY THAT METHOD

Publications (3)

Publication Number	Publication Date
NO20023985L NO20023985L (no)	2002-08-21
NO20023985D0 NO20023985D0 (no)	2002-08-21
NO328694B1 true NO328694B1 (no)	2010-04-26

Family

ID=8167925

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
NO20023985A NO328694B1 (no)	2000-02-22	2002-08-21	Fremgangsmate for fremstilling av rekombinant hCG med spesifikk biologisk aktivitet fra en prove

Country Status (32)

Country	Link
US (2)	US7297777B2 (ja)
EP (2)	EP1257564B1 (ja)
JP (1)	JP4588960B2 (ja)
KR (1)	KR100806911B1 (ja)
CN (1)	CN100482679C (ja)
AT (1)	ATE406378T1 (ja)
AU (2)	AU783320B2 (ja)
BG (1)	BG65807B1 (ja)
BR (1)	BRPI0108556B8 (ja)
CA (1)	CA2399020A1 (ja)
CY (1)	CY1108460T1 (ja)
CZ (1)	CZ303144B6 (ja)
DE (1)	DE60135541D1 (ja)
DK (1)	DK1257564T3 (ja)
EA (1)	EA006605B1 (ja)
EE (1)	EE05644B1 (ja)
ES (1)	ES2307585T3 (ja)
HK (1)	HK1052016A1 (ja)
HR (1)	HRP20020650B1 (ja)
HU (2)	HU228935B1 (ja)
IL (2)	IL151308A0 (ja)
ME (1)	ME00296B (ja)
MX (1)	MXPA02007871A (ja)
NO (1)	NO328694B1 (ja)
PL (1)	PL205149B1 (ja)
PT (1)	PT1257564E (ja)
RS (1)	RS50741B (ja)
SI (1)	SI1257564T1 (ja)
SK (1)	SK287146B6 (ja)
UA (2)	UA78488C2 (ja)
WO (1)	WO2001062773A1 (ja)
ZA (1)	ZA200206109B (ja)

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Publication number	Priority date	Publication date	Assignee	Title
EA006605B1 (ru) *	2000-02-22	2006-02-24	Апплайд Резеч Системз Арс Холдинг Н.В.	СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО чХГ
JP2007513192A (ja) *	2004-10-04	2007-05-24	ノベタイド，リミティド	ペプチド用の対イオン交換法
CN1958603B (zh) *	2005-11-04	2010-05-05	上海天伟生物制药有限公司	一种人绒毛膜促性腺素的纯化方法
WO2008007993A1 (fr) *	2006-07-11	2008-01-17	Koval, Anton Alexandrovich	Utilisation d'une hormone chorionique ou lutéinisante destinée à la prévention ou au traitement de l'hypogonadisme lié à l'âge
CN101397339B (zh) *	2008-09-24	2012-07-25	上海天伟生物制药有限公司	糖蛋白中去除/灭活病毒的方法
TWI604850B (zh) *	2009-10-05	2017-11-11	菲瑞茵國際中心股份有限公司	藥學製劑
CN101792481B (zh) *	2010-03-12	2012-05-30	丽珠医药集团股份有限公司	一种绒毛膜***的纯化方法
WO2012120535A2 (en) *	2011-02-03	2012-09-13	Sanzyme Limited	A composition comprising highly purified chorionic gonadotropin, it's formulation and uses of the same
WO2012131306A1 (en)	2011-03-31	2012-10-04	Ferring B.V.	Pharmaceutical preparation
WO2013102921A2 (en) *	2011-11-17	2013-07-11	Sanzyme Limited	Hcg - newer treatment modality for type 2 diabetes mellitus (t2dm)
CN103288950A (zh) *	2012-12-28	2013-09-11	青岛九龙生物医药有限公司	改进柱分离工序洗涤液和洗脱液配比去除杂质的方法
CN104101656A (zh) *	2013-04-01	2014-10-15	上海天伟生物制药有限公司	一种***的纯度测定方法
US20150065426A1 (en) *	2013-08-27	2015-03-05	Professional Compounding Centers Of America	Testosterone Booster Transdermal Compositions
CN105968185A (zh) *	2016-07-20	2016-09-28	宁波人健药业集团股份有限公司	一种绒促性素纯化方法
CN111233999B (zh) *	2020-03-21	2024-02-23	上海浦东明炎生物技术有限公司	一种从人尿中提取人绒毛膜***的方法
CN113398251B (zh) *	2021-07-30	2022-08-05	宁波人健药业集团股份有限公司	一种重组人绒毛膜***冻干粉针剂及其制备方法

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Publication number	Priority date	Publication date	Assignee	Title
DK0461200T3 (da) *	1989-02-21	1997-03-10	Univ Washington	Modificerede former af reproduktionshormoner
IT1250075B (it) *	1991-12-18	1995-03-30	Serono Cesare Ist Ricerca	Composizioni farmaceutiche contenenti gonadotropine.
IT1254370B (it) *	1992-05-22	1995-09-14	Sorin Biomedica Spa	Procedimento per la purificazione di proteine da sistemi cellulari.
DK0814841T3 (da)	1995-03-21	2002-03-04	Applied Research Systems	Flydende hCG-formuleringer
EA002474B1 (ru) *	1996-02-20	2002-06-27	Апплайд Резеч Системз Арс Холдинг Н.В.	Гибридный белок, образующий гетеродимеры, способ его получения и использования
IT1287138B1 (it) *	1996-11-07	1998-08-04	Ibsa Inst Biochimique Sa	Procedimento per la separazione e purificazione di fsh e lh
WO1998056806A1 (en)	1997-06-12	1998-12-17	The Regents Of The University Of California	A TRANSCRIPTION FACTOR COACTIVATOR PROTEIN, p/CIP
ES2287976T3 (es) *	1997-06-13	2007-12-16	Genentech, Inc.	Recuperacion de proteinas mediante cromatografia seguida de filtracion sobre una capa cargada.
US6583109B1 (en) *	1997-06-24	2003-06-24	Robert C. Gallo	Therapeutic polypeptides from β-hCG and derivatives
EA006605B1 (ru) *	2000-02-22	2006-02-24	Апплайд Резеч Системз Арс Холдинг Н.В.	СПОСОБ ОЧИСТКИ РЕКОМБИНАНТНОГО чХГ

2001
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2002
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2003
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- 2006-01-06 AU AU2006200059A patent/AU2006200059A1/en not_active Abandoned
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Also Published As

Publication number	Publication date
DE60135541D1 (de)	2008-10-09
EA200200892A1 (ru)	2003-02-27
EA006605B1 (ru)	2006-02-24
BR0108556A (pt)	2003-01-28
US20070299001A1 (en)	2007-12-27
ATE406378T1 (de)	2008-09-15
EE200200469A (et)	2004-04-15
BG65807B1 (bg)	2009-12-31
HRP20020650B1 (en)	2011-02-28
PT1257564E (pt)	2008-09-10
CY1108460T1 (el)	2014-04-09
HUP0204231A2 (hu)	2003-03-28
IL151308A (en)	2009-11-18
CZ20022855A3 (cs)	2002-11-13
JP4588960B2 (ja)	2010-12-01
IL151308A0 (en)	2003-04-10
BRPI0108556B8 (pt)	2021-05-25
NO20023985L (no)	2002-08-21
RS50741B (sr)	2010-08-31
CA2399020A1 (en)	2001-08-30
UA78488C2 (en)	2007-04-10
SK287146B6 (sk)	2010-01-07
CN100482679C (zh)	2009-04-29
HU228935B1 (en)	2013-06-28
US7297777B2 (en)	2007-11-20
EP1257564B1 (en)	2008-08-27
CZ303144B6 (cs)	2012-05-02
UA86938C2 (uk)	2009-06-10
YU60202A (sh)	2005-09-19
AU2006200059A1 (en)	2006-02-02
KR20030001359A (ko)	2003-01-06
ME00296B (me)	2011-05-10
HRP20020650A2 (en)	2004-12-31
SK12062002A3 (sk)	2002-12-03
US20030104553A1 (en)	2003-06-05
WO2001062773A1 (en)	2001-08-30
NO20023985D0 (no)	2002-08-21
PL357508A1 (en)	2004-07-26
HU1300063D0 (hu)	2003-03-28
SI1257564T1 (sl)	2008-12-31
MEP38908A (en)	2011-02-10
DK1257564T3 (da)	2008-10-06
MXPA02007871A (es)	2003-02-10
BRPI0108556B1 (pt)	2015-04-14
ZA200206109B (en)	2003-07-31
KR100806911B1 (ko)	2008-02-22
PL205149B1 (pl)	2010-03-31
EP1752466A3 (en)	2012-10-10
CN1404485A (zh)	2003-03-19
JP2003524015A (ja)	2003-08-12
AU2680401A (en)	2001-09-03
AU783320B2 (en)	2005-10-13
EE05644B1 (et)	2013-04-15
BG107000A (en)	2003-04-30
HK1052016A1 (en)	2003-08-29
EP1257564A1 (en)	2002-11-20
ES2307585T3 (es)	2008-12-01
EP1752466A2 (en)	2007-02-14
HUP0204231A3 (en)	2005-09-28

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Date	Code	Title	Description
2008-01-14	CREP	Change of representative	Representative=s name: ONSAGERS AS, POSTBOKS 6963 ST OLAVS PLASS, 0130 OS
2021-02-08	MK1K	Patent expired

Publication	Publication Date	Title
US20070299001A1 (en)	2007-12-27	PURIFIED hCG
US20060079460A1 (en)	2006-04-13	Purified LH
KR101860830B1 (ko)	2018-05-24	당단백질 정제 방법
IL248293A (en)	2017-04-30	Gonadotropin purification process
CN110563832A (zh)	2019-12-13	一种高纯度重组促***纯化方法