JP2001518786A - 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 - Google Patents
糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換Info
- Publication number
- JP2001518786A JP2001518786A JP54235598A JP54235598A JP2001518786A JP 2001518786 A JP2001518786 A JP 2001518786A JP 54235598 A JP54235598 A JP 54235598A JP 54235598 A JP54235598 A JP 54235598A JP 2001518786 A JP2001518786 A JP 2001518786A
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- JP
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- Prior art keywords
- dna
- transformation
- gene
- transformants
- transformed
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Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biophysics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
Description
Claims (1)
- 【特許請求の範囲】 1. 形質転換された糸状菌の生成方法であって、 (1)糸状菌に導入されるべき少なくとも1つの発現可能な遺伝子DNAフラ グメントを、まずAgrobacterium tumefaciensのベクター中に、そのベ クター中に存在するT−DNAボーダー間にクローニングし; (2)T−DNAボーダー間に上記DNAを含むベクターを、DNA中にvi r領域を含むAgrobacterium tumefaciens株に導入し; (3)vir誘導化合物の添加によって上記Agrobacterium tumefaciensから 上記DNAフラグメントを含むT−DNAを放出させ、形質転換される べき糸状菌とともにこのAgrobacterium tumefaciens株をインキュベー トし;そして (4)形質転換された糸状菌を、導入されたDNA又はその発現生成物の特徴 に依存して非形質転換糸状菌から選択し、そして場合によっては形質転 換された糸状菌を培養すること を特徴とする方法。 2. 糸状菌が、Eumycotaの群に属する、請求項1記載の方法。 3. 糸状菌が、Ascomycotina、Basidiomycotina、Deuteromycotina、Mastigomyc otina、及びZygomycotina菌類亜門からなる群から選択される、請求項1記 載の方法。 4. DNAフラグメントが、所望の遺伝子の複数のコピーを含む、請求項1記載 の方法。 5. DNAフラグメントが、糸状菌ゲノムの選択された遺伝子座に組み込まれる ,請求項1記載の方法。 6. DNAフラグメントが、糸状菌ゲノムのpyrG遺伝子座(A.nigerについ てはpyrA遺伝子座及びNeurospora crassaについてはpyr4遺伝子座 として知られる)に組み込まれる、請求項5記載の方法。 7. 形質転換された糸状菌が、T−DNAボーダーを含め、望ましくない細菌D NA配列を含まない、請求項5記載の方法。 8. T−DNAボーダーを含め、望ましくない細菌DNA配列を含まない、請求 項7記載のアグロバクテリウム媒介性形質転換によって得られる形質転換さ れた糸状菌。 9. 場合によっては所望のタンパクの生産を誘導することを含む、請求項8記載 の形質転換された糸状菌の培養方法。 10.DNAフラグメントが、糸状菌ゲノム中にランダムに組み込まれる、請求項 1記載の方法。 11.T−DNAボーダー配列の1又は2以上の部分を含む、請求項10記載のア グロバクテリウム媒介性形質転換によって得られる形質転換された糸状菌。 12.場合によっては所望のタンパクの生産を誘導することを含む、請求項11記 載の形質転換された糸状菌の培養方法。
Applications Claiming Priority (5)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
EP97201022A EP0870835A1 (en) | 1997-04-07 | 1997-04-07 | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus Aspergillus |
EP97201022.7 | 1997-12-22 | ||
EP97204062.0 | 1997-12-22 | ||
EP97204062 | 1997-12-22 | ||
PCT/EP1998/001914 WO1998045455A1 (en) | 1997-04-07 | 1998-03-24 | Agrobacterium mediated transformation of moulds, in particular those belonging to the genus aspergillus |
Publications (2)
Publication Number | Publication Date |
---|---|
JP2001518786A true JP2001518786A (ja) | 2001-10-16 |
JP4307563B2 JP4307563B2 (ja) | 2009-08-05 |
Family
ID=26146337
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
JP54235598A Expired - Fee Related JP4307563B2 (ja) | 1997-04-07 | 1998-03-24 | 糸状菌、特にアスペルギルス属に属する糸状菌のアグロバクテリウム媒介性形質転換 |
Country Status (12)
Country | Link |
---|---|
US (1) | US6255115B1 (ja) |
EP (1) | EP0973917B1 (ja) |
JP (1) | JP4307563B2 (ja) |
CN (1) | CN1151264C (ja) |
AU (1) | AU7428398A (ja) |
BR (1) | BR9807941A (ja) |
CA (1) | CA2286307A1 (ja) |
DE (1) | DE69822142T2 (ja) |
ES (1) | ES2216284T3 (ja) |
ID (1) | ID22929A (ja) |
MY (1) | MY121328A (ja) |
WO (1) | WO1998045455A1 (ja) |
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-
1998
- 1998-03-24 ID IDW991162A patent/ID22929A/id unknown
- 1998-03-24 JP JP54235598A patent/JP4307563B2/ja not_active Expired - Fee Related
- 1998-03-24 WO PCT/EP1998/001914 patent/WO1998045455A1/en active IP Right Grant
- 1998-03-24 EP EP98921416A patent/EP0973917B1/en not_active Expired - Lifetime
- 1998-03-24 CA CA002286307A patent/CA2286307A1/en not_active Abandoned
- 1998-03-24 ES ES98921416T patent/ES2216284T3/es not_active Expired - Lifetime
- 1998-03-24 AU AU74283/98A patent/AU7428398A/en not_active Abandoned
- 1998-03-24 US US09/402,628 patent/US6255115B1/en not_active Expired - Fee Related
- 1998-03-24 BR BR9807941-7A patent/BR9807941A/pt not_active IP Right Cessation
- 1998-03-24 CN CNB988058960A patent/CN1151264C/zh not_active Expired - Fee Related
- 1998-03-24 DE DE69822142T patent/DE69822142T2/de not_active Expired - Lifetime
- 1998-04-06 MY MYPI98001542A patent/MY121328A/en unknown
Cited By (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
JP2014512824A (ja) * | 2011-04-25 | 2014-05-29 | エコベイティブ デザイン エルエルシー | 脱水菌糸体からなる部材の作製方法及びそれによって作製される製品 |
CN112680365A (zh) * | 2021-02-02 | 2021-04-20 | 吉林农业大学 | 一种白僵菌用液体培养基及白僵菌菌剂制备方法 |
Also Published As
Publication number | Publication date |
---|---|
EP0973917B1 (en) | 2004-03-03 |
ID22929A (id) | 1999-12-16 |
AU7428398A (en) | 1998-10-30 |
BR9807941A (pt) | 2000-02-22 |
CN1259167A (zh) | 2000-07-05 |
EP0973917A1 (en) | 2000-01-26 |
CA2286307A1 (en) | 1998-10-15 |
WO1998045455A1 (en) | 1998-10-15 |
US6255115B1 (en) | 2001-07-03 |
JP4307563B2 (ja) | 2009-08-05 |
CN1151264C (zh) | 2004-05-26 |
DE69822142T2 (de) | 2005-03-03 |
MY121328A (en) | 2006-01-28 |
DE69822142D1 (de) | 2004-04-08 |
ES2216284T3 (es) | 2004-10-16 |
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