DE69822142D1 - Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus - Google Patents

Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus

Info

Publication number
DE69822142D1
DE69822142D1 DE69822142T DE69822142T DE69822142D1 DE 69822142 D1 DE69822142 D1 DE 69822142D1 DE 69822142 T DE69822142 T DE 69822142T DE 69822142 T DE69822142 T DE 69822142T DE 69822142 D1 DE69822142 D1 DE 69822142D1
Authority
DE
Germany
Prior art keywords
agrobacterium
transforming
mold
aspergillus
generation
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69822142T
Other languages
English (en)
Other versions
DE69822142T2 (de
Inventor
Godelieve Beijersbergen
Paul-Rijks Universitei Bundock
Johannes Gouka
Groot Marcellus Johannes A De
Jan Hooykaas
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Unilever NV
Original Assignee
Unilever NV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from EP97201022A external-priority patent/EP0870835A1/de
Application filed by Unilever NV filed Critical Unilever NV
Application granted granted Critical
Publication of DE69822142D1 publication Critical patent/DE69822142D1/de
Publication of DE69822142T2 publication Critical patent/DE69822142T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69822142T 1997-04-07 1998-03-24 Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus Expired - Lifetime DE69822142T2 (de)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
EP97201022A EP0870835A1 (de) 1997-04-07 1997-04-07 Transformation von Schimmeln durch Agrobacterium, insbesondere von der Gattung Aspergillus
EP97201022 1997-04-07
EP97204062 1997-12-22
EP97204062 1997-12-22
PCT/EP1998/001914 WO1998045455A1 (en) 1997-04-07 1998-03-24 Agrobacterium mediated transformation of moulds, in particular those belonging to the genus aspergillus

Publications (2)

Publication Number Publication Date
DE69822142D1 true DE69822142D1 (de) 2004-04-08
DE69822142T2 DE69822142T2 (de) 2005-03-03

Family

ID=26146337

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69822142T Expired - Lifetime DE69822142T2 (de) 1997-04-07 1998-03-24 Transformation von schimmeln durch agrobacterium, insbesondere von der gattung aspergillus

Country Status (12)

Country Link
US (1) US6255115B1 (de)
EP (1) EP0973917B1 (de)
JP (1) JP4307563B2 (de)
CN (1) CN1151264C (de)
AU (1) AU7428398A (de)
BR (1) BR9807941A (de)
CA (1) CA2286307A1 (de)
DE (1) DE69822142T2 (de)
ES (1) ES2216284T3 (de)
ID (1) ID22929A (de)
MY (1) MY121328A (de)
WO (1) WO1998045455A1 (de)

Families Citing this family (67)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
US7220542B2 (en) 2000-07-17 2007-05-22 Van Den Brink Johannes Maarten Expression cloning in filamentous fungi
WO2001044482A2 (en) 1999-12-16 2001-06-21 Cropdesign N.V. Optimized t-dna transfer and vectors therefor
ES2302739T3 (es) 2000-06-28 2008-08-01 The Penn State Research Foundation Metodo para la transformacion del agaricus bisporus.
EP2295559B1 (de) 2002-08-16 2015-09-16 Danisco US Inc. Neuartige Hypocrea jecorina CBH1-Cellulasevariante mit erhöhter thermischer Stabilität, die eine Substitution oder Deletion an Position T255 umfasst
CN102604916A (zh) * 2002-09-10 2012-07-25 金克克国际有限公司 使用高浓度糖混合物诱导基因表达
US7208585B2 (en) * 2002-09-18 2007-04-24 Genencor International, Inc. Protein purification
WO2004072225A2 (en) * 2003-02-12 2004-08-26 Ramot At Tel Aviv University Ltd. Transgenic fungi expressing bcl-2 and methods of using bcl-2 or portions thereof for improving biomass production, survival, longevity, stress resistance and pathogenicity of fungi
JP2007534294A (ja) * 2003-03-21 2007-11-29 ジェネンコー・インターナショナル・インク Cbh1相同体及び変異体cbh1セルラーゼ
WO2004097001A2 (en) * 2003-04-29 2004-11-11 Genencor International, Inc. Novel bacillus 029cel cellulase
DK1618182T3 (da) 2003-04-30 2013-10-14 Genencor Int Ny bacillus-cellulase MHKcel
WO2004099369A2 (en) * 2003-04-30 2004-11-18 Genencor International, Inc. Novel bacillus bagcel cellulase
WO2004101759A2 (en) * 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme lip2
WO2004101760A2 (en) 2003-05-12 2004-11-25 Genencor International, Inc. Novel lipolytic enzyme elip
EP1625202A4 (de) 2003-05-12 2010-10-20 Genencor Int Neues lipolytisches enzym lip1
US20050181509A1 (en) * 2004-02-12 2005-08-18 The Penn State Research Foundation Dual selection based, targeted gene disruption method for fungi and fungus-like organisms
GB0405659D0 (en) * 2004-03-12 2004-04-21 Horticulture Res Internat Altered expression in filamentous fungi
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
JP5114194B2 (ja) 2004-03-25 2013-01-09 ジェネンコー・インターナショナル・インク セルラーゼ融合タンパク質及びこれをコードする異種セルラーゼ融合構築体
WO2006071598A1 (en) * 2004-12-23 2006-07-06 Genencor International, Inc. Neutral cellulase catalytic core and method of producing same
CN101094918B (zh) * 2004-12-30 2014-05-14 金克克国际有限公司 酸性真菌蛋白酶
DK2302049T3 (en) 2004-12-30 2017-05-01 Danisco Us Inc CBH2 CELLULASE VARIETIES OF HYPOCREA JECORINA
WO2006122023A1 (en) 2005-05-09 2006-11-16 The Samuel Roberts Noble Foundation, Inc. Agrobacterium transformation of stolons
CN100370016C (zh) * 2006-04-21 2008-02-20 贵州大学 对根癌农杆菌有抑制作用的黑曲霉菌株及其用途
US7968691B2 (en) * 2006-08-23 2011-06-28 Danisco Us Inc. Pullulanase variants with increased productivity
CA2661808A1 (en) 2006-08-29 2008-03-06 Danisco Us, Inc., Genencor Division Compositions and methods for improved protein production
US20100093061A1 (en) * 2006-12-20 2010-04-15 Bodie Elizabeth A Assays for Improved Fungal Strains
EP2147104B1 (de) * 2007-05-21 2015-09-09 Danisco US Inc. Verwendung einer asparaginprotease (nsp24)-signalsequenz zur heterologen proteinexpression
US20090221030A1 (en) * 2007-11-01 2009-09-03 Kai Bao Signal sequences and co-expressed chaperones for improving protein production in a host cell
US20120270302A1 (en) * 2011-04-25 2012-10-25 Eben Bayer Method for Making Dehydrated Mycelium Elements and Product Made Thereby
US7973215B2 (en) 2008-04-11 2011-07-05 Mycomagic Biotechnology Co., Ltd. Method for the introduction of a heterologous polynucleotide into a mushroom
WO2009149202A2 (en) 2008-06-06 2009-12-10 Danisco Us Inc., Genencor Division Compositions and methods comprising cellulase variants with reduced affinity to non-cellulosic materials
US8907165B2 (en) 2009-04-22 2014-12-09 Medicine In Need Corporation Production of provitamin A carotenoids in mushrooms and uses thereof
CN104862293A (zh) 2009-06-03 2015-08-26 丹尼斯科美国公司 具有改善的表达、活性和/或稳定性的纤维素酶变体,及其用途
CN105316303B (zh) 2009-11-20 2019-05-31 丹尼斯科美国公司 具有改善的特性的β-葡萄糖苷酶I变体
JP2013533737A (ja) 2010-06-03 2013-08-29 ダニスコ・ユーエス・インク 糸状菌宿主株及びdna構築物並びにその使用方法
WO2011158623A1 (ja) * 2010-06-17 2011-12-22 キッコーマン株式会社 麹菌染色体における大領域重複の作製方法
JP2014502144A (ja) 2010-10-06 2014-01-30 ビーピー・コーポレーション・ノース・アメリカ・インコーポレーテッド バリアントcbhiポリペプチド
CN102206666B (zh) * 2011-04-15 2013-02-06 山东省农业科学院农业资源与环境研究所 一种质粒载体pLGF及其应用
CN104053780A (zh) 2011-12-09 2014-09-17 丹尼斯科美国公司 用于在微生物中生产蛋白质的来自枯草芽孢杆菌的核糖体启动子
JP6081245B2 (ja) * 2012-04-07 2017-02-15 キッコーマン株式会社 麹菌染色体における任意領域の重複転座の作製方法
CN102839214A (zh) * 2012-09-06 2012-12-26 许汉鹏 筛选真核细胞基因组内特定基因调控相关机制和分子的方法
EP3290515A1 (de) 2012-12-12 2018-03-07 Danisco US Inc. Varianten von cellobiohydrolasen
CN103834681B (zh) * 2013-11-26 2016-01-13 中国计量学院 一种农杆菌介导遗传转化稻曲菌的方法
US9914932B2 (en) 2014-11-13 2018-03-13 Battelle Memorial Institute Agrobacterium-mediated transformation of lipomyces
KR20170116159A (ko) 2015-02-19 2017-10-18 다니스코 유에스 인크. 향상된 단백질 발현
MX2017016625A (es) 2015-07-07 2018-05-15 Danisco Us Inc Induccion de la expresion genica usando una mezcla de azucar de alta concentracion.
KR20180043365A (ko) 2015-09-02 2018-04-27 듀폰 뉴트리션 바이오사이언시즈 에이피에스 글리코시드 하이돌라제 및 동물에서의 병원성 감염의 예방 및/또는 치료에서 이의 용도
CN109071615A (zh) 2016-03-04 2018-12-21 丹尼斯科美国公司 用于在微生物中产生蛋白质的工程化核糖体启动子
CN109642226A (zh) 2016-06-30 2019-04-16 丹尼斯科美国公司 天冬氨酸蛋白酶
AU2017332665B2 (en) 2016-09-23 2021-12-02 International N&H Denmark Aps Use of low pH active alpha-1,4/1,6-glycoside hydrolases as a feed additive for ruminants to enhance starch digestion
KR102593668B1 (ko) 2016-10-04 2023-10-24 다니스코 유에스 인크. 유도 기질 부재 하의 사상 진균 세포에서의 단백질 생산
US20190330577A1 (en) 2016-12-21 2019-10-31 Dupont Nutrition Biosciences Aps Methods of using thermostable serine proteases
US20200071685A1 (en) 2017-03-06 2020-03-05 Dupont Nutrition Biosciences Aps Novel fungal fucosidases and their use in preventing and/or treating a pathogenic infection in an animal
CN110621778A (zh) 2017-03-15 2019-12-27 丹尼斯科美国公司 胰蛋白酶样丝氨酸蛋白酶及其用途
BR112019018983A2 (pt) 2017-03-15 2020-04-14 Dupont Nutrition Biosci Aps métodos de uso de uma serina protease archaeal
MX2019010815A (es) 2017-03-15 2020-02-20 Dupont Nutrition Biosci Aps Serina proteasas de tipo tripsina novedosas y sus usos.
MX2021000149A (es) 2018-07-06 2021-03-25 Dupont Nutrition Biosci Aps Aditivos para piensos que contienen xilanasa para piensos para animales a base de cereales.
JP2022553854A (ja) 2019-11-08 2022-12-26 ダニスコ・ユーエス・インク タンパク質生産性が増強された表現型を含む糸状菌株及びその方法
EP4139332A1 (de) 2020-04-22 2023-03-01 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in filamentösen pilzzellen
CN112680365B (zh) * 2021-02-02 2023-01-31 吉林农业大学 一种白僵菌用液体培养基及白僵菌菌剂制备方法
CN117716039A (zh) 2021-07-19 2024-03-15 丹尼斯科美国公司 用于增强真菌细胞中的蛋白质产生的组合物和方法
CN117881772A (zh) 2021-08-20 2024-04-12 丹尼斯科美国公司 编码新型核酸酶的多核苷酸、其组合物及其从蛋白质制剂中消除dna的方法
WO2023102315A1 (en) 2021-12-01 2023-06-08 Danisco Us Inc. Compositions and methods for enhanced protein production in fungal cells
CN114875046B (zh) * 2022-05-30 2023-07-18 福州大学 一种丝状真菌复制子
WO2024102556A1 (en) 2022-11-11 2024-05-16 Danisco Us Inc. Filamentous fungal strains comprising enhanced protein productivity phenotypes and methods thereof
WO2024137350A2 (en) 2022-12-19 2024-06-27 Danisco Us Inc. Recombinant fungal strains and methods thereof for producing consistent proteins

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2063592C (en) 1989-07-07 2003-01-21 Marco L. F. Giuseppin Process for preparing a protein by a fungus transformed by multicopy integration of an expression vector
DK0672149T3 (da) * 1991-12-09 2001-11-05 Unilever Nv Fremgangsmåde til fremstilling/udskilning af et protein med en transformeret skimmelsvamp ved anvendelse af ekspressions/udskilningsregulerende regioner afledt af et Aspergillus-endoxylanase II-gen

Also Published As

Publication number Publication date
CN1259167A (zh) 2000-07-05
ES2216284T3 (es) 2004-10-16
ID22929A (id) 1999-12-16
JP2001518786A (ja) 2001-10-16
EP0973917A1 (de) 2000-01-26
AU7428398A (en) 1998-10-30
CN1151264C (zh) 2004-05-26
US6255115B1 (en) 2001-07-03
BR9807941A (pt) 2000-02-22
DE69822142T2 (de) 2005-03-03
EP0973917B1 (de) 2004-03-03
MY121328A (en) 2006-01-28
CA2286307A1 (en) 1998-10-15
WO1998045455A1 (en) 1998-10-15
JP4307563B2 (ja) 2009-08-05

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Legal Events

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8332 No legal effect for de
8370 Indication of lapse of patent is to be deleted
8328 Change in the person/name/address of the agent

Representative=s name: MEISSNER, BOLTE & PARTNER GBR, 80538 MUENCHEN