HK1171790A1 - 用於 蛋白表達的細胞培養基 - Google Patents

用於 蛋白表達的細胞培養基

Info

Publication number
HK1171790A1
HK1171790A1 HK12112610.1A HK12112610A HK1171790A1 HK 1171790 A1 HK1171790 A1 HK 1171790A1 HK 12112610 A HK12112610 A HK 12112610A HK 1171790 A1 HK1171790 A1 HK 1171790A1
Authority
HK
Hong Kong
Prior art keywords
adamts
culture medium
cell culture
protein expression
adamts protein
Prior art date
Application number
HK12112610.1A
Other languages
English (en)
Inventor
Leopold Grillberger
Alexandra Spenger
Meinhard Hasslacher
Rana Grillberger
Manfred Reiter
Original Assignee
Baxalta GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Baxalta GmbH filed Critical Baxalta GmbH
Publication of HK1171790A1 publication Critical patent/HK1171790A1/zh

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/48Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6421Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from mammals
    • C12N9/6489Metalloendopeptidases (3.4.24)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0681Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06Animal cells or tissues; Human cells or tissues
    • C12N5/0602Vertebrate cells
    • C12N5/0684Cells of the urinary tract or kidneys
    • C12N5/0686Kidney cells
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y304/00Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/24Metalloendopeptidases (3.4.24)
    • C12Y304/24087ADAMTS13 endopeptidase (3.4.24.87)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/12Light metals, i.e. alkali, alkaline earth, Be, Al, Mg
    • C12N2500/14Calcium; Ca chelators; Calcitonin
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/05Inorganic components
    • C12N2500/10Metals; Metal chelators
    • C12N2500/20Transition metals
    • C12N2500/22Zinc; Zn chelators
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/30Organic components
    • C12N2500/38Vitamins
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/90Serum-free medium, which may still contain naturally-sourced components
    • C12N2500/95Protein-free medium and culture conditions
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2500/00Specific components of cell culture medium
    • C12N2500/99Serum-free medium
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00Genetically modified cells
    • C12N2510/02Cells for production
HK12112610.1A 2009-07-31 2012-12-06 用於 蛋白表達的細胞培養基 HK1171790A1 (zh)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US23047709P 2009-07-31 2009-07-31
PCT/US2010/044020 WO2011014838A1 (en) 2009-07-31 2010-07-30 Cell culture medium for adamts protein expression

Publications (1)

Publication Number Publication Date
HK1171790A1 true HK1171790A1 (zh) 2013-04-05

Family

ID=42668009

Family Applications (1)

Application Number Title Priority Date Filing Date
HK12112610.1A HK1171790A1 (zh) 2009-07-31 2012-12-06 用於 蛋白表達的細胞培養基

Country Status (22)

Country Link
US (7) US8313926B2 (zh)
EP (1) EP2459702B1 (zh)
JP (5) JP5451884B2 (zh)
KR (5) KR101756354B1 (zh)
CN (2) CN104962539B (zh)
AU (1) AU2010278751B2 (zh)
BR (2) BR122021005965B1 (zh)
CA (1) CA2769361A1 (zh)
CO (1) CO6501191A2 (zh)
EA (1) EA023193B1 (zh)
ES (1) ES2583258T3 (zh)
HK (1) HK1171790A1 (zh)
HR (1) HRP20160783T1 (zh)
HU (1) HUE029110T2 (zh)
IN (1) IN2012DN00801A (zh)
MX (2) MX2012001261A (zh)
NZ (1) NZ597727A (zh)
PL (1) PL2459702T3 (zh)
PT (1) PT2459702T (zh)
SG (1) SG178194A1 (zh)
SI (1) SI2459702T1 (zh)
WO (1) WO2011014838A1 (zh)

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KR101756354B1 (ko) * 2009-07-31 2017-07-26 백스터 인터내셔널 인코포레이티드 Adamts 단백질 발현을 위한 세포 배양 배지
PL2591094T3 (pl) 2010-07-08 2019-02-28 Baxalta GmbH Sposób otrzymywania rekombinowanego adamts13 w linii komórkowej
KR102108663B1 (ko) 2011-07-01 2020-05-07 암젠 인크 포유동물 세포 배양
EP2906683B1 (en) 2012-10-15 2017-05-31 Bristol-Myers Squibb Company Mammalian cell culture processes for protein production
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CA3031740A1 (en) 2016-08-04 2018-02-08 Baxalta Incorporated Use of adamts13 for treating, ameliorating and/or preventing vaso-occlusive crisis in sickle cell disease, acute lung injury and/or acute respiratory distress syndrome
ES2948849T3 (es) * 2016-11-04 2023-09-20 Takeda Pharmaceuticals Co Métodos de purificación de virus adenoasociado
US11391725B2 (en) * 2018-03-16 2022-07-19 Genzyme Corporation Methods for improving cell viability in a production bioreactor
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BR112021025769A2 (pt) 2019-12-06 2022-04-12 Regeneron Pharma Composições de proteína anti-vegf e métodos para a produção das mesmas
EP4127158A2 (en) 2020-04-02 2023-02-08 Takeda Pharmaceutical Company Limited Adamts13 variant, compositions, and uses thereof
WO2021234458A1 (en) 2020-05-22 2021-11-25 Takeda Pharmaceutical Company Limited Adamts13 compositions and methods for treating and diagnosing complications of coronavirus disease
CN111808822B (zh) * 2020-07-02 2024-01-12 北京百普赛斯生物科技股份有限公司 细胞培养用补料液及提高重组hek293细胞蛋白表达量的方法

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Also Published As

Publication number Publication date
US20110086413A1 (en) 2011-04-14
CN102549145B (zh) 2015-05-20
US8759026B2 (en) 2014-06-24
HUE029110T2 (en) 2017-02-28
JP5936232B2 (ja) 2016-06-22
EP2459702B1 (en) 2016-05-04
MX2012001261A (es) 2012-05-22
KR20150044981A (ko) 2015-04-27
US20190071660A1 (en) 2019-03-07
US20200308566A1 (en) 2020-10-01
US9441216B2 (en) 2016-09-13
BR112012002151B1 (pt) 2021-07-06
US20130071906A1 (en) 2013-03-21
JP7083059B2 (ja) 2022-06-09
SI2459702T1 (sl) 2016-11-30
US10724024B2 (en) 2020-07-28
JP6511007B2 (ja) 2019-05-08
JP2018093881A (ja) 2018-06-21
KR101756354B1 (ko) 2017-07-26
SG178194A1 (en) 2012-03-29
US11254921B2 (en) 2022-02-22
ES2583258T3 (es) 2016-09-20
JP2021112210A (ja) 2021-08-05
CN102549145A (zh) 2012-07-04
EA023193B1 (ru) 2016-05-31
KR20150138425A (ko) 2015-12-09
JP2014061005A (ja) 2014-04-10
EA201270224A1 (ru) 2012-09-28
JP2016144471A (ja) 2016-08-12
US20170051270A1 (en) 2017-02-23
JP5451884B2 (ja) 2014-03-26
CA2769361A1 (en) 2011-02-03
NZ597727A (en) 2013-08-30
US8313926B2 (en) 2012-11-20
US20140329294A1 (en) 2014-11-06
CN104962539B (zh) 2019-09-17
CN104962539A (zh) 2015-10-07
IN2012DN00801A (zh) 2015-06-26
PT2459702T (pt) 2016-08-02
JP2013500724A (ja) 2013-01-10
KR20170081747A (ko) 2017-07-12
AU2010278751A1 (en) 2012-02-09
KR20170140411A (ko) 2017-12-20
US20150329848A1 (en) 2015-11-19
US9127265B2 (en) 2015-09-08
KR20120046301A (ko) 2012-05-09
BR122021005965B1 (pt) 2022-01-25
MX344970B (es) 2017-01-12
HRP20160783T1 (hr) 2016-08-12
WO2011014838A1 (en) 2011-02-03
KR101573972B1 (ko) 2015-12-02
JP2019201661A (ja) 2019-11-28
EP2459702A1 (en) 2012-06-06
KR101912552B1 (ko) 2018-10-26
US10072254B2 (en) 2018-09-11
PL2459702T3 (pl) 2016-12-30
BR112012002151A2 (pt) 2020-03-03
KR101574056B1 (ko) 2015-12-02
JP6883071B2 (ja) 2021-06-09
KR101808496B1 (ko) 2017-12-12
AU2010278751B2 (en) 2014-05-15
JP6567707B2 (ja) 2019-08-28
CO6501191A2 (es) 2012-08-15

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