KR880701246A - 재조합 페포 계면활성제 단백질 - Google Patents
재조합 페포 계면활성제 단백질Info
- Publication number
- KR880701246A KR880701246A KR1019870701256A KR870701256A KR880701246A KR 880701246 A KR880701246 A KR 880701246A KR 1019870701256 A KR1019870701256 A KR 1019870701256A KR 870701256 A KR870701256 A KR 870701256A KR 880701246 A KR880701246 A KR 880701246A
- Authority
- KR
- South Korea
- Prior art keywords
- protein
- dna
- asp
- mixture
- carbohydrate
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P11/00—Drugs for disorders of the respiratory system
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/785—Alveolar surfactant peptides; Pulmonary surfactant peptides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K38/00—Medicinal preparations containing peptides
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- General Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biochemistry (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biophysics (AREA)
- Medicinal Chemistry (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Pulmonology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Toxicology (AREA)
- Physics & Mathematics (AREA)
- Gastroenterology & Hepatology (AREA)
- Public Health (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Pharmacology & Pharmacy (AREA)
- Veterinary Medicine (AREA)
- Animal Behavior & Ethology (AREA)
- Analytical Chemistry (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Peptides Or Proteins (AREA)
- Cosmetics (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Saccharide Compounds (AREA)
Abstract
내용 없음
Description
본 내용은 요부공개 건이므로 전문내용을 수록하지 않았음
Claims (12)
- 제 위치에서 정상적으로 수반되는 단백질 유리 페포계면활성제 단백질(ASP)에 있어서, 사람 SP-18 DNA와 사람 SP-5 DNA및 그의 프로세스된 형태를 포함하는데 의해 코드되는 단백질로 이루어지는 군으로부터 선택되는 페포계면활성제 단백질(ASP)
- 제1항에 있어서, 제2도, 제3도, 제5도 또는 제6도에 도시된 DNA 또는 그와 실질적으로 동등한 DNA에 의해 코드되는 것을 특징으로 하는 단백질.
- 제1항의 단백질을 코드하는 재조합 DNA서열에 있어서, DNA는 제1항의 단백질을 정상적으로 수반하는 단백질을 코드하는 DNA가 유리된 것을 특징으로 하는 재조합 DNA서열.
- 제3항에 있어서, 적당한 재조합 숙주세포에서 상기 서열의 발현에 효과적인 제어 서열에 활발하게 연결되는 것을특징으로 하는 DNA.
- 제3항의 DNA또는 제4항의 발현시스템으로 형질전환되는 제조합숙주세포.
- 제5항의 세포를 배양하는 것을 포함하는 ASP제조방법.
- 제6항의 방법에 의해 제조된 재조합 ASP.
- 32K ASP 단백질의 정제 방법에 있어서, (a) 32K 단백질이 탄수화물 결합 지지에 흡수되어 있는 조건하에서 혼합물과 탄수화물 결합지지의 접촉; 및 (b) 단백질이 결합되지 않은 조건하에서 32K 단백질의 용리에 의해 탄수화물 결합 지지를 사용하여 상기 32K단백질을 함유하는 혼합물에 탄수화물 친화성 크로마토그래피를 수행하는 것으로 이루어지는 32K ASP 단백질의 정제방법.
- 제8항에 있어서, 탄수화물 친화성은 만노스 잔기에 의해 공급되고, 32K단백질의 지지에의 결합은 칼슘이온의 존재하에 수행되며 상기 32K단백질의 용리는 칼슘이온의 존재하에 수행되며 상기 32K 단백질의 용리는 칼슘이온 농도를 감소시키는 조건하에 있는 것을 특징으로 하는 방법.
- 제8항의 방법에 의하여 정제되는 ASP 단백질.
- 인지질 제제와, 가장 적당하게는 약제학적 수용가능한 부형제와의 혼합물 형태로 제1항 또는 제7항 또는 제10항의 단백질을 포함하는 조성물로서 포유류에서 호흡을 위한 (RDS)치료에 효과적인 약제학적 조성물.
- 인지질 제제와, 가장 적당하게는 약제학적 수용가능한 부형제와의 혼합물 형태의 32K ASP 단백질의 효과적인 양과의 혼합물 형태로 제1항 또는 제7항 또는 제10항의 단백질을 포함하는 조성물로서 포유류에서 RDS치료에 효과적인 약제학적 조성물.※ 참고사항 : 최초출원 내용에 의하여 공개하는 것임.
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US06/857,715 US4933280A (en) | 1984-12-11 | 1986-04-30 | Recombinant DNA sequence encoding Alveolar Surfactant Protein |
US857715 | 1986-04-30 | ||
PCT/US1987/000978 WO1987006588A1 (en) | 1986-04-30 | 1987-04-30 | Recombinant alveolar surfactant protein |
Publications (1)
Publication Number | Publication Date |
---|---|
KR880701246A true KR880701246A (ko) | 1988-07-26 |
Family
ID=25326586
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
KR1019870701256A KR880701246A (ko) | 1986-04-30 | 1987-04-30 | 재조합 페포 계면활성제 단백질 |
Country Status (14)
Country | Link |
---|---|
US (1) | US4933280A (ko) |
EP (1) | EP0251449B1 (ko) |
JP (3) | JPH0813839B2 (ko) |
KR (1) | KR880701246A (ko) |
AT (1) | ATE118035T1 (ko) |
AU (1) | AU591566B2 (ko) |
CA (1) | CA1340611C (ko) |
DE (1) | DE3751033T2 (ko) |
DK (1) | DK173173B1 (ko) |
ES (1) | ES2072850T3 (ko) |
GR (1) | GR3015913T3 (ko) |
HK (1) | HK1005887A1 (ko) |
IL (1) | IL82364A (ko) |
WO (1) | WO1987006588A1 (ko) |
Families Citing this family (20)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3761394A (en) * | 1969-12-31 | 1973-09-25 | Texaco Inc | Catalytic cracking of naphtha |
US5840527A (en) * | 1984-12-11 | 1998-11-24 | Byk Gulden Lomberg Chemische Fabrik Gmbh | Recombinant alveolar surfactant protein |
EP0240550A4 (en) * | 1985-09-26 | 1989-09-19 | Genetics Inst | LUNG SURFACE ACTIVE PROTEINS. |
US5013720A (en) * | 1986-05-06 | 1991-05-07 | Abbott Laboratories | SAP-6-Val proteins and methods |
JPH01501282A (ja) * | 1986-12-08 | 1989-05-11 | ホイツトセツト,ジエフリー エイ. | 肺胞疎水性界面活性物質関連タンパク質 |
US5260273A (en) * | 1988-01-06 | 1993-11-09 | The Scripps Research Institute | Pulmonary surfactant protein and related polypeptide |
US6613734B2 (en) | 1988-01-06 | 2003-09-02 | The Scripps Research Institute | Peptides-containing liposomal surfactants |
US5164369A (en) * | 1988-01-06 | 1992-11-17 | The Scripps Research Institute | Pulmonary surfactant protein and related polypeptide |
EP0428615A4 (en) * | 1988-08-11 | 1991-11-13 | California Biotechnology, Inc. | Method for stabilizing heterologous protein expression and vectors for use therein |
SE8803713D0 (sv) * | 1988-10-18 | 1988-10-18 | Kabigen Ab | Biologically active lipoprotein and its use |
DE69125750D1 (de) * | 1990-05-21 | 1997-05-28 | Abbott Lab | Fettsäure-Oberflächenaktives-Lungenprotein Konjate |
GB9120005D0 (en) * | 1991-09-19 | 1991-11-06 | Wellcome Found | Method of administering phospholipid dispersions |
US5272252A (en) * | 1991-11-04 | 1993-12-21 | Merrell Dow Pharmaceuticals Inc. | Synthetic lung surfactant having antioxidant properties |
DE4418936A1 (de) * | 1994-05-31 | 1996-02-08 | Byk Gulden Lomberg Chem Fab | Polypeptid |
US6031075A (en) * | 1996-02-05 | 2000-02-29 | Children's Hospital Medical Center | Mature alveolar SP-B and a process for producing the same |
EP1216052A1 (en) | 1999-09-16 | 2002-06-26 | Byk Gulden Lomberg Chemische Fabrik GmbH | Combination of c1-inh and lung surfactant for the treatment of respiratory disorders |
AU2002338882B2 (en) | 2001-10-11 | 2008-04-03 | Fraunhofer-Gesellschaft Zur Foerderung Der Angewandten Forschung E.V. | Novel use of pulmonary surfactant |
ATE457736T1 (de) | 2004-08-06 | 2010-03-15 | Nycomed Gmbh | Zusammensetzung aus einem pulmonalen surfactant und einem von tnf stammenden peptid |
US7951781B2 (en) | 2006-11-02 | 2011-05-31 | University Of Iowa Research Foundation | Methods and compositions related to PLUNC surfactant polypeptides |
CN102271666A (zh) | 2008-12-10 | 2011-12-07 | Paka肺部医药公司 | 用于向肺脏递送药物的方法和组合物 |
Family Cites Families (6)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4312860A (en) * | 1980-10-24 | 1982-01-26 | Regents Of The University Of California | Lung surfactant compositions |
US4361509A (en) * | 1981-12-14 | 1982-11-30 | Scripps Clinic And Research Foundation | Ultrapurification of factor VIII using monoclonal antibodies |
US4562003A (en) * | 1984-10-26 | 1985-12-31 | California Biotechnology, Inc. | Monoclonal antibodies against alveolar surfactant protein |
US4659805A (en) * | 1984-12-11 | 1987-04-21 | California Biotechnology, Inc. | Recombinant alveolar surfactant protein |
JPS63503222A (ja) * | 1986-05-06 | 1988-11-24 | サイオス ノバ インコーポレイテッド | 肺の疎水性界面活性物質に結合した分子量6,000ダルトンのタンパク質及びその多量体 |
JPH01501282A (ja) * | 1986-12-08 | 1989-05-11 | ホイツトセツト,ジエフリー エイ. | 肺胞疎水性界面活性物質関連タンパク質 |
-
1986
- 1986-04-30 US US06/857,715 patent/US4933280A/en not_active Expired - Lifetime
-
1987
- 1987-04-28 IL IL82364A patent/IL82364A/xx unknown
- 1987-04-29 CA CA000535980A patent/CA1340611C/en not_active Expired - Lifetime
- 1987-04-30 EP EP87303919A patent/EP0251449B1/en not_active Expired - Lifetime
- 1987-04-30 WO PCT/US1987/000978 patent/WO1987006588A1/en unknown
- 1987-04-30 JP JP62503195A patent/JPH0813839B2/ja not_active Expired - Lifetime
- 1987-04-30 AU AU74802/87A patent/AU591566B2/en not_active Ceased
- 1987-04-30 DE DE3751033T patent/DE3751033T2/de not_active Expired - Fee Related
- 1987-04-30 ES ES87303919T patent/ES2072850T3/es not_active Expired - Lifetime
- 1987-04-30 KR KR1019870701256A patent/KR880701246A/ko not_active Application Discontinuation
- 1987-04-30 AT AT87303919T patent/ATE118035T1/de not_active IP Right Cessation
-
1992
- 1992-12-17 DK DK199201517A patent/DK173173B1/da not_active IP Right Cessation
-
1995
- 1995-04-25 GR GR950401034T patent/GR3015913T3/el unknown
- 1995-09-08 JP JP7231858A patent/JP2584962B2/ja not_active Expired - Fee Related
-
1996
- 1996-08-08 JP JP8210325A patent/JP2766806B2/ja not_active Expired - Lifetime
-
1998
- 1998-06-08 HK HK98105033A patent/HK1005887A1/xx not_active IP Right Cessation
Also Published As
Publication number | Publication date |
---|---|
HK1005887A1 (en) | 1999-01-29 |
DE3751033T2 (de) | 1995-08-10 |
AU591566B2 (en) | 1989-12-07 |
EP0251449A2 (en) | 1988-01-07 |
JP2766806B2 (ja) | 1998-06-18 |
US4933280A (en) | 1990-06-12 |
DK173173B1 (da) | 2000-02-28 |
DE3751033D1 (de) | 1995-03-16 |
GR3015913T3 (en) | 1995-07-31 |
JPH0813839B2 (ja) | 1996-02-14 |
IL82364A (en) | 1993-08-18 |
DK151792A (da) | 1992-12-17 |
JP2584962B2 (ja) | 1997-02-26 |
CA1340611C (en) | 1999-06-29 |
EP0251449B1 (en) | 1995-02-01 |
AU7480287A (en) | 1987-11-24 |
ATE118035T1 (de) | 1995-02-15 |
JPH08188598A (ja) | 1996-07-23 |
JPH09117290A (ja) | 1997-05-06 |
EP0251449A3 (en) | 1989-04-19 |
IL82364A0 (en) | 1987-10-30 |
JPH01500350A (ja) | 1989-02-09 |
ES2072850T3 (es) | 1995-08-01 |
WO1987006588A1 (en) | 1987-11-05 |
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WITN | Application deemed withdrawn, e.g. because no request for examination was filed or no examination fee was paid |