DK2620496T3 - Alfa-amylase - Google Patents
Alfa-amylase Download PDFInfo
- Publication number
- DK2620496T3 DK2620496T3 DK12153083.6T DK12153083T DK2620496T3 DK 2620496 T3 DK2620496 T3 DK 2620496T3 DK 12153083 T DK12153083 T DK 12153083T DK 2620496 T3 DK2620496 T3 DK 2620496T3
- Authority
- DK
- Denmark
- Prior art keywords
- polynucleotide
- polypeptide
- vector
- seq
- enzyme
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2414—Alpha-amylase (3.2.1.1.)
- C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
-
- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D13/00—Finished or partly finished bakery products
- A21D13/06—Products with modified nutritive value, e.g. with modified starch content
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- A—HUMAN NECESSITIES
- A21—BAKING; EDIBLE DOUGHS
- A21D—TREATMENT, e.g. PRESERVATION, OF FLOUR OR DOUGH, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS; PRESERVATION THEREOF
- A21D8/00—Methods for preparing or baking dough
- A21D8/02—Methods for preparing dough; Treating dough prior to baking
- A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
- A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01001—Alpha-amylase (3.2.1.1)
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Nutrition Science (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Bakery Products And Manufacturing Methods Therefor (AREA)
- Peptides Or Proteins (AREA)
Claims (18)
1. Polynukleotid, der koder for et polypeptid med alfa-amylaseaktivitet, der omfatter: (a) en polynukleotidsekvens, der koder for et polypeptid med en aminosyresekvens ifølge aminosyrerne 34 til 719 af SEQ ID NO: 2; eller (b) en polynukleotidsekvens ifølge nukleotiderne 100 til 2157 af SEQ ID NO: 1 eller SEQ ID NO: 3; eller (c) en polynukleotidsekvens ifølge SEQ ID NO: 1 eller SEQ ID NO: 3 .
2. Polynukleotid ifølge krav 1, hvor polynukleotidet er dannet af Alicyclobacillus pohliae NCIMB14276.
3. Vektor, der omfatter polynukleotidsekvensen ifølge krav 1 eller 2.
4. Vektor ifølge krav 3, som er en ekspressionsvektor, hvor polynukleotidsekvensen ifølge krav 1 eller 2 er operabelt koblet med mindst én regulatorsekvens, der åbner mulighed for ekspression af polynukleotidsekvensen i en egnet værtscelle.
5. Vektor ifølge krav 4, hvor den egnede værtscelle er en Aspergillus-, Bacillus-, Chrysosporium-, Escherichia-, Kluyveromyces-, Penicillium-, Pseudomonas-, Saccharomyces-, Streptomyces- eller Talaromyces-art, fortrinsvis en Bacillus subtilis-, Bacillus amyloliquefaciens-, Bacillus licheniformis-, Escherichia coli-, Aspergillus niger- eller Aspergillus oryzae-art.
6. Rekombinant værtscelle, der omfatter polynukleotidet ifølge krav 1 eller 2 eller omfatter vektoren ifølge et hvilket som helst af kravene 3 til 5.
7. Rekombinant værtscelle ifølge krav 6, der er i stand til at udtrykke eller overudtrykke polynukleotidet eller vektoren.
8. Fremgangsmåde til fremstilling af polynukleotidet ifølge krav 1 eller 2 eller vektoren ifølge et hvilket som helst af kravene 3 til 5, der omfatter trinene dyrkning af en værtscelle, der er transformeret med polynukleotidet eller vektoren, og isolering af polynukleotidet eller vektoren fra værtscellen.
9. Alfa-amylasepolypeptid, der omfatter: (a) en aminosyresekvens ifølge aminosyrerne 34 til 719 af SEQ ID NO: 2; eller (b) en aminosyresekvens, der kodes af et polynukleotid ifølge nukleotiderne 100 til 2157 af SEQ ID NO: 1 eller SEQ ID NO: 3.
10. Polypeptid ifølge krav 9, der kan opnås ved ekspression af polynukleotidet ifølge krav 1 eller 2 eller vektoren ifølge et hvilket som helst af kravene 3 til 5 i en passende værtscelle.
11. Fremgangsmåde til fremstilling af polypeptidet ifølge krav 9 eller 10, der omfatter dyrkning af en rekombinant værtscelle ifølge krav 6 eller 7 under betingelser, der åbner mulighed for ekspression af polynukleotidet ifølge krav 1 eller 2 eller vektoren ifølge krav 3 til 5, og eventuelt indvinding af det kodede polypeptid fra cellen eller dyrkningsmediet.
12. Anvendelse af polypeptidet ifølge krav 9 eller 10 til fødevarefremstilling.
13. Anvendelse ifølge krav 12 til fremstilling af et bagt produkt, fortrinsvis et brød eller en kage.
14. Enzymsammensætning, der omfatter polypeptidet ifølge krav 9 eller 10 og et eller flere bestanddele, der er udvalgt fra gruppen, der består af mælkepulver, gluten, granuleret fedt, et yderligere enzym, en aminosyre, et salt, en oxidant, såsom ascorbinsyre, bromat og azodicarbonamid, et reduceringsmiddel, såsom L-cystein, et emulgeringsmiddel, såsom monoglycerider, diglycerider, glycerolmonostearat, natriumstearoyllactylat, calciumstearoyllactylat, polyglycerolestere af fedtsyrer og DATEM, gummier, såsom guargummi og xanthangummi, smagsstoffer, syrer, såsom citronsyre og propionsyre, stivelse, modificeret stivelse, gluten, fugtighedsbevarende midler, såsom glycerol, og konserveringsmidler.
15. Enzymsammensætning ifølge krav 14, hvor det yderligere enzym er et lipolytisk enzym, fortrinsvis en phospholipase.
16. Fremgangsmåde til fremstilling af en dej, der omfatter trinet kombinering af polypeptidet ifølge krav 9 eller 10 eller sammensætningen ifølge krav 14 eller 15 og mindst én dejbestanddel.
17. Dej, der omfatter polypeptidet ifølge krav 9 eller 10 eller sammensætningen ifølge krav 14 eller 15.
18. Fremgangsmåde til fremstilling af et bagt produkt, der omfatter trinet bagning af dejen ifølge krav 17.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| EP12153083.6A EP2620496B1 (en) | 2012-01-30 | 2012-01-30 | Alpha-amylase |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2620496T3 true DK2620496T3 (da) | 2015-09-07 |
Family
ID=48094809
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK12153083.6T DK2620496T3 (da) | 2012-01-30 | 2012-01-30 | Alfa-amylase |
Country Status (12)
| Country | Link |
|---|---|
| US (2) | US8426182B1 (da) |
| EP (2) | EP2620496B1 (da) |
| CN (1) | CN104220593B (da) |
| AR (1) | AR089840A1 (da) |
| AU (1) | AU2013214397A1 (da) |
| BR (1) | BR112014018610A2 (da) |
| CA (1) | CA2861148A1 (da) |
| CL (1) | CL2014002030A1 (da) |
| DK (1) | DK2620496T3 (da) |
| EA (1) | EA201400850A1 (da) |
| ES (1) | ES2546630T3 (da) |
| WO (1) | WO2013113665A1 (da) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP6325535B2 (ja) | 2012-06-27 | 2018-05-16 | ノボザイムス アクティーゼルスカブ | 米の調理方法 |
| US9301533B2 (en) * | 2013-03-01 | 2016-04-05 | Dsm Ip Assets B.V. | Alpha-amylase variants |
| EP3102669B1 (en) | 2014-02-07 | 2019-06-12 | DSM IP Assets B.V. | Improved bacillus host |
| WO2016030448A1 (en) * | 2014-08-28 | 2016-03-03 | Dsm Ip Assets B.V. | Alicyclobacillus pohliae alpha-amylase variants |
| BE1022042B1 (nl) | 2014-09-29 | 2016-02-08 | Puratos Nv | Verbeterde cakebeslagsoorten |
| DE102015113641A1 (de) * | 2015-08-18 | 2017-02-23 | Bode Chemie Gmbh | Desinfektionsmittel mit organischen Säuren |
| CN106479996B (zh) * | 2015-08-24 | 2021-03-02 | 丰益(上海)生物技术研发中心有限公司 | 一种新型的淀粉酶 |
| CN107227284B (zh) * | 2017-05-27 | 2020-06-09 | 江南大学 | 一种发酵小分子透明质酸的重组兽疫链球菌 |
| CN108690841B (zh) * | 2018-02-08 | 2021-10-01 | 河南科技学院 | 一种mPEG-Mal5000修饰β-淀粉酶的方法 |
| CN108517330A (zh) * | 2018-04-12 | 2018-09-11 | 江南大学 | 一种敲除ybbD提高重组枯草芽孢杆菌几丁寡糖的方法 |
| CN109182240B (zh) * | 2018-09-18 | 2021-06-25 | 绿康生化股份有限公司 | 地衣芽孢杆菌DW2ΔphoP在杆菌肽生产中的应用 |
| CN111220604B (zh) * | 2019-12-06 | 2022-03-15 | 东北农业大学 | 一种肉制品中总淀粉含量的测定方法 |
Family Cites Families (25)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS6030488B2 (ja) | 1982-11-10 | 1985-07-17 | 協和醗酵工業株式会社 | 生地の改良剤およびそれを含有してなる生地 |
| DK135983D0 (da) | 1983-03-25 | 1983-03-25 | Novo Industri As | Maltogen amylaseenzymprodukt og fremgangsmade til dets fremstilling og anvendelse |
| JPS6078529A (ja) | 1983-10-07 | 1985-05-04 | 協和醗酵工業株式会社 | 生地 |
| JPS62111629A (ja) | 1985-11-09 | 1987-05-22 | キユーピー株式会社 | ケ−キの製造方法 |
| JPS63258528A (ja) | 1987-04-15 | 1988-10-26 | キユーピー株式会社 | スポンジケ−キの製造方法 |
| DK474589D0 (da) | 1989-09-27 | 1989-09-27 | Novo Nordisk As | Fremgangsmaade til fremstilling af bageriprodukter |
| USRE38507E1 (en) | 1989-09-27 | 2004-04-27 | Novozymes A/S | Antistaling process and agent |
| DK0426211T3 (da) | 1989-09-29 | 1994-01-31 | Unilever Plc | Næringsmiddelprodukt indeholdende tørret lyso-phospholipoprotein |
| ATE151948T1 (de) | 1993-03-31 | 1997-05-15 | Gist Brocades Nv | Hefeformulierung zur herstellung von backwaren |
| DK76893D0 (da) | 1993-06-28 | 1993-06-28 | Novo Nordisk As | |
| PT659344E (pt) | 1993-12-24 | 2001-12-28 | Dsm Nv | Composicoes de levedura seca traducao |
| CN1148442C (zh) | 1996-12-09 | 2004-05-05 | 诺维信公司 | 利用来自具有磷脂酶a和/或b活性的丝状真菌的磷脂酶降低含有大量非水合磷的食用油中的含磷成分 |
| CN1292028B (zh) | 1998-02-27 | 2013-08-14 | 诺维信公司 | 麦芽α淀粉酶变体 |
| NZ511340A (en) | 1998-11-27 | 2003-07-25 | Novozymes As | Lipolytic enzyme variants |
| PT1204326E (pt) | 1999-08-17 | 2005-07-29 | Dsm Ip Assets Bv | Composicao liquida para melhoria de pao |
| US20030190399A1 (en) | 2000-09-28 | 2003-10-09 | Schooneveld-Bergmans Margot Elisabeth Francoise | Liquid bread improving compositions |
| US20040091601A1 (en) | 2000-12-20 | 2004-05-13 | Parnell Marie Diane | Liquid yeast compositions |
| AU2003203139B2 (en) | 2002-01-16 | 2007-11-08 | Novozymes A/S | Lipolytic enzyme variants and method for their production |
| AU2003303934A1 (en) | 2002-05-30 | 2004-09-09 | Dsm Ip Assets B.V. | Novel pectinases and uses thereof |
| GB0305685D0 (en) * | 2003-03-12 | 2003-04-16 | Danisco | Enzyme |
| NZ542924A (en) | 2003-04-28 | 2008-01-31 | Novozymes As | Method of producing a phospholipase which comprises processing an expressed fungal peptide so as to cleave off a peptide from the C- terminal end and/or a peptide from the N-terminal end to obtain a core peptide |
| US7888489B2 (en) | 2005-01-24 | 2011-02-15 | Dsm Ip Assets B.V. | Method for producing a compound of interest in a filamentous fungal cell |
| EP2035561A1 (en) | 2006-06-29 | 2009-03-18 | DSMIP Assets B.V. | A method for achieving improved polypeptide expression |
| DK2527430T3 (da) * | 2007-06-07 | 2015-04-13 | Novozymes As | Fremgangsmåde til fremstilling af et dejbaseret produkt |
| ES2429492T3 (es) | 2008-02-29 | 2013-11-15 | Dsm Ip Assets B.V. | Lipasas con alta especificidad hacia ácidos grasos de cadena corta y usos de las mismas |
-
2012
- 2012-01-30 EP EP12153083.6A patent/EP2620496B1/en active Active
- 2012-01-30 ES ES12153083.6T patent/ES2546630T3/es active Active
- 2012-01-30 DK DK12153083.6T patent/DK2620496T3/da active
- 2012-07-23 US US13/532,072 patent/US8426182B1/en active Active
-
2013
- 2013-01-29 US US14/374,945 patent/US20140377407A1/en not_active Abandoned
- 2013-01-29 EP EP13701469.2A patent/EP2809781A1/en not_active Withdrawn
- 2013-01-29 WO PCT/EP2013/051608 patent/WO2013113665A1/en active Application Filing
- 2013-01-29 CA CA2861148A patent/CA2861148A1/en not_active Abandoned
- 2013-01-29 AR ARP130100265A patent/AR089840A1/es unknown
- 2013-01-29 BR BR112014018610A patent/BR112014018610A2/pt not_active IP Right Cessation
- 2013-01-29 AU AU2013214397A patent/AU2013214397A1/en not_active Abandoned
- 2013-01-29 EA EA201400850A patent/EA201400850A1/ru unknown
- 2013-01-29 CN CN201380007360.8A patent/CN104220593B/zh not_active Expired - Fee Related
-
2014
- 2014-07-30 CL CL2014002030A patent/CL2014002030A1/es unknown
Also Published As
| Publication number | Publication date |
|---|---|
| AR089840A1 (es) | 2014-09-24 |
| ES2546630T3 (es) | 2015-09-25 |
| US8426182B1 (en) | 2013-04-23 |
| AU2013214397A1 (en) | 2014-07-24 |
| BR112014018610A2 (pt) | 2017-07-04 |
| CL2014002030A1 (es) | 2015-04-10 |
| CA2861148A1 (en) | 2013-08-08 |
| CN104220593B (zh) | 2016-08-17 |
| US20140377407A1 (en) | 2014-12-25 |
| CN104220593A (zh) | 2014-12-17 |
| EA201400850A1 (ru) | 2014-12-30 |
| EP2620496B1 (en) | 2015-06-03 |
| EP2620496A1 (en) | 2013-07-31 |
| WO2013113665A1 (en) | 2013-08-08 |
| EP2809781A1 (en) | 2014-12-10 |
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